Locally Different Endothelial Nitric Oxide Synthase Protein Levels in Ascending Aortic Aneurysms of Bicuspid and Tricuspid Aortic Valve by Salah A. Mohamed, Arlo Radtke, Roza Saraei, Joern Bullerdiek, Hajar Sorani, Rolf Nimzyk, Antje Karluss, Hans H. Sievers, Gazanfer Belge

“…Aims. Dysregulated expression of the endothelial nitric oxide synthase (eNOS) is observed in aortic aneurysms associated with bicuspid aortic valve (BAV). …”
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Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions by Marina G. Gobbo, Nishtman Dizeyi, Per-Anders Abrahamsson, Per-Anders Bertilsson, Viviane Sanches Masitéli, Eloisa Zanin Pytlowanciv, Sebastião R. Taboga, Rejane M. Góes

“…MLT treatment impeded apoptosis p=0.02 and augmented proliferation p=0.0008 and PCNA content in prostate following long-term diabetes due to restoration of testosterone levels and expression of melatonin receptor type 1B. The effect of MLT (500 µM, 5 mM, and 10 mM) on androgen-dependent (22Rv1) and androgen-independent (PC3) cancer cells and human prostate epithelial cells (PNTA1) under normal and hyperglycemic conditions (HG, 450 mg/dL) was analyzed. …”
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Beyond Hormone Receptors: liquid biopsy tools to unveil new clinical meanings and empower therapeutic decision-making in Luminal-like metastatic breast cancer by Brenno Pastò, Riccardo Vida, Arianna Dri, Lorenzo Foffano, Serena Della Rossa, Lorenzo Gerratana, Fabio Puglisi

“…Immunohistochemical (IHC) tissue profiling is a standard practice in the management of metastatic breast cancer (mBC), that enables the identification of distinct biological phenotypes based on hormone receptors' expression. Luminal-like tumors primarily benefit from a first line treatment strategy combining endocrine therapy and cyclin-dependent kinase 4/6 inhibitors. …”
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ROS-regulated SUR1-TRPM4 drives persistent activation of NLRP3 inflammasome in microglia after whole-brain radiation by Yuan Chang, Yihua He, Di Wang, Kunxue Zhang, Yuzhen Zhang, Zhentong Li, Shuxin Zeng, Sheng Xiao, Suyue Pan, Kaibin Huang

“…Moreover, ROS up-regulated ten-eleven translocation 2 (TET2) to enhance TRPM4 expression by mediating the demethylation of the gene promoter, thereby facilitating the assembly of SUR1-TRPM4 in microglia. …”
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Blockade of Wnt/β-Catenin Pathway Aggravated Silica-Induced Lung Inflammation through Tregs Regulation on Th Immune Responses by Wujing Dai, Fangwei Liu, Chao Li, Yiping Lu, Xiaowei Lu, Sitong Du, Ying Chen, Dong Weng, Jie Chen

“…To investigate the role of Wnt/β-catenin pathway, we used lentivirus expressing β-catenin shRNA to block the Wnt/β-catenin pathway by intratracheal instillation to the mice model of silicosis. …”
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Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis by Yewei Jia, Rui Li, Yixuan Li, Katerina Kachler, Xianyi Meng, Andreas Gießl, Yi Qin, Fulin Zhang, Ning Liu, Darja Andreev, Georg Schett, Aline Bozec

“…In addition, HIF1α pathway was shown as an upstream regulator, providing a potential target for modulating HMOX1 expression and influencing autophagy-dependent ferroptosis. …”
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The regulatory effects of (p)ppGpp and indole on cAMP synthesis in <i>Escherichia coli</i> cells by N. M. Kashevarova, E. A. Khaova, A. G. Tkachenko

“…Bacterial stress adaptive response is formed due to changes in the cell gene expression profile in response to alterations in environmental conditions through the functioning of regulatory networks. …”
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From homogeneity to heterogeneity: Refining stochastic simulations of gene regulation by Seok Joo Chae, Seolah Shin, Kangmin Lee, Seunggyu Lee, Jae Kyoung Kim

“…Our findings indicate that when transcription factors diffuse slowly, these simulations fail to accurately capture gene expression, highlighting the necessity to account for spatial heterogeneity. …”
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MDTR: a knowledge-guided interpretable representation for quantifying liver toxicity at transcriptomic level by Inyoung Sung, Sangseon Lee, Dongmin Bang, Dongmin Bang, Jungseob Yi, Sunho Lee, Sun Kim, Sun Kim, Sun Kim, Sun Kim

“…Despite accumulating drug-induced transcriptome data such as LINCS, analyzing such transcriptome data upon drug treatment is a challenging task because the perturbation of expression is dose and time dependent. In addition, the mechanisms of drug toxicity are known only as literature information, not in a computable form.MethodsTo address these challenges, we propose a Multi-Dimensional Transcriptomic Ruler (MDTR) that quantifies the degree of DILI at the transcriptome level. …”
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Insight into the role of antioxidant in microbial lignin degradation: Ascorbic acid as a fortifier of lignin-degrading enzymes by Aipeng Li, Weimin Wang, Shuqi Guo, Changzhi Li, Xinying Wang, Qiang Fei

“…Proteomics analysis suggested that the enhancement in enzyme activity was more likely to attributed to the reinforcement of AA on oxidative protein folding and transportation, rather than changes in enzyme expression. Conclusions The addition of AA enhanced the performance of enzymes responsible for lignin degradation in terms of enzyme activity, degradation rate, lignin structural change, and product mapping. …”
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Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior by E. Y. Bazhenova, D. V. Fursenko, N. V. Khotskin, I. E. Sorokin, A. V. Kulikov

“…The lethal yellow (AY) mutation causes ectopic expression of agouti protein in the brain. Mice heterozygous for AY mutation (AY/a) are obese compared to their wild-type littermates (a/a). …”
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Possibility of Using NO Modulators for Pharmacocorrection of Endothelial Dysfunction After Prenatal Hypoxia by Igor Belenichev, Olena Popazova, Oleh Yadlovskyi, Nina Bukhtiyarova, Victor Ryzhenko, Sergii Pavlov, Valentyn Oksenych, Oleksandr Kamyshnyi

“…Levels of sEPCR, Tie2 tyrosine kinase, VEGF-B, SOD1/Cu-Zn SOD, GPX4, and GPX1 in the heart’s cytosolic homogenate were assessed using ELISA. The expression of VEGF and VEGF-B mRNA was analyzed via real-time polymerase chain reaction, and the nuclear area of myocardial microvessel endothelial cells was evaluated morphometrically. …”
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Exploring pre-exposure prophylaxis (PrEP) modality preferences among black cisgender women attending family planning clinics in Chicago via a cross-sectional mixed-methods study by Sadia Haider, Amy K Johnson, Emily Ott, Eleanor E Friedman, Agustina Pandiani, Amy Moore, Isa Alvarez, Catherine Desmarais

“…Both injections and combination pills were popular among participants, with 64.5% and 67.3% expressing interest in these forms of PrEP, respectively. …”
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Triiodothyronine treatment in mice improves stroke outcome and reduces blood–brain barrier damage by Daniel Ullrich, Dagmar Führer, Heike Heuer, Steffen Mayerl, Steffen Haupeltshofer, Linda-Isabell Schmitt, Markus Leo, Rebecca D Szepanowski, Tim Hagenacker, Markus Schwaninger, Christoph Kleinschnitz, Friederike Langhauser

“…In-depth investigations further revealed reduced cerebral edema and diminished blood–brain barrier leakage, indicated by reduced extravasation of Evans blue and diminished aquaporin-4 expression. Conclusion: Our findings suggest that T3 may be a promising intervention for ischemic stroke in the acute phase.…”
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Anti-Inflammatory Effect Fraction of Bletilla striata and Its Protective Effect on LPS-Induced Acute Lung Injury by Chunchun Zhang, Dandan Ning, Jieli Pan, Cheng Chen, Chengxian Gao, Zhishan Ding, Fusheng Jiang, Meiya Li

“…Western blot analysis demonstrated the increased expressions of iNOS, COX-2, and NF-κB p65 in the LPS treatment group, all of which were ameliorated by EFBS pretreatment. …”
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Improving dexamethasone drug loading and efficacy in treating rheumatoid arthritis via liposome: Focusing on inflammation and molecular mechanisms by Mohammad Yasin Zamanian, Hamidreza Zafari, Maria K. Osminina, Alla A. Skakodub, Raed Fanoukh Aboqader Al‐Aouadi, Maryam Golmohammadi, Nikta Nikbakht, Iman Fatemi

“…Additionally, liposomal DEX can induce the expression of anti‐inflammatory cytokines like interleukin‐10 (IL‐10), which has significant anti‐inflammatory and immunoregulatory properties. …”
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Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids by Margherita Maioli, Valentina Basoli, Sara Santaniello, Sara Cruciani, Alessandro Palmerio Delitala, Roberto Pinna, Egle Milia, Regina Grillari-Voglauer, Vania Fontani, Salvatore Rinaldi, Roberta Muggironi, Gianfranco Pigliaru, Carlo Ventura

“…Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. …”
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Autophagy-Enhancing Properties of <i>Hedyotis diffusa</i> Extracts in HaCaT Keratinocytes: Potential as an Anti-Photoaging Cosmetic Ingredient by Qiwen Zheng, Xiangji Jin, Trang Thi Minh Nguyen, Eun-Ji Yi, Se-Jig Park, Gyeong-Seon Yi, Su-Jin Yang, Tae-Hoo Yi

“…Cellular respiration was further evaluated with the Seahorse XFp Analyzer, and RT-PCR and Western blotting were used to analyze the impact of HD on mitochondrial autophagy-related gene expression and signaling pathways. Our findings indicate that HD promotes autophagy by modulating the PI3K/AKT/mTOR and PINK/PARK2 pathways, which stabilizes mitochondrial quality, maintains MMP and Ca²⁺ balance, and reduces cytochrome c release. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…Efferocytosis promoted macrophage polarization from the M1 to the M2 phenotype, reduced the expression of proinflammatory cytokines, and decreased the levels of inflammation and oxidative stress. …”
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Genetic Mapping for Seed Aging Tolerance Under Multiple Environments in Sweet Corn by Yanchao Du, Jianting Lin, Haoxuan Jiang, Huating Zhao, Xuebiao Zhang, Ruigang Wang, Faqiang Feng

“…Through Gene Ontology enrichment, gene annotation, and expression profiling clustering analyses, 12 positive candidate genes linked to seed aging tolerance were identified. …”
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