The effect of thioredoxin and prochymosin coexpression on the refolding of recombinant alpaca chymosin by S. V. Belenkaya, D. N. Shcherbakov, A. I. Chapoval, T. I. Esina, V. V. Elchaninov

“…To achieve this goal, on the basis of the pET32a plasmid, an expression vector was constructed containing the thioredoxin A gene fused to the N-terminal sequence of the marker enzyme zymogen, alpaca prochymosin. …”
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Loss-of-function variant in MAGT1 leading to XMEN disease in a Colombian patient with a common variable immunodeficiency by Sebastián Gutiérrez-Hincapié, Julio César Orrego, José Luis Franco, Claudia M. Trujillo-Vargas

“…The clinical phenotype was extracted from the clinical records. NKG2D expression in natural killer cells was evaluated by flow cytometry. …”
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Engineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET by Andreas Evers, Simon Krah, Deniz Demir, Ramona Gaa, Desislava Elter, Christian Schroeter, Stefan Zielonka, Nicolas Rasche, Julia Dotterweich, Christine Knuehl, Achim Doerner

“…The resulting biparatopic anti-c-MET ADCs were comparably active on c-MET expressing tumor cell lines as REGN5093 exatecan DAR6 ADC. …”
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Associations between Tissue Visfatin/Nicotinamide, Phosphoribosyltransferase (Nampt), Retinol Binding Protein-4, and Vaspin Concentrations and Insulin Resistance in Morbidly Obese... by Zeynep Goktas, Shannon Owens, Mallory Boylan, David Syn, Chwan-Li Shen, Debra B. Reed, Susan San Francisco, Shu Wang

“…Visfatin/Nampt, vaspin, and RBP-4 gene expression levels in tissues were measured using real-time PCR. …”
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Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. Migration and invasion of multiple myeloma cells were determined with the wound-healing and transwell assays, respectively. …”
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Pre-injection of exosomes can significantly suppress ovarian cancer growth by activating the immune system in mice by Yuanyuan Wang, Changyi Zhang, Huimin Zeng, Liangliang Wang, Zanhong Wang, Zhiqiang Han

“…Surprisingly, we also observed high expression of programmed death ligand 1 (PD-L1) in the omentum. …”
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Small molecules and heat treatments reverse vernalization via epigenetic modification in Arabidopsis by Nana Otsuka, Ryoya Yamaguchi, Hikaru Sawa, Naoya Kadofusa, Nanako Kato, Yasuyuki Nomura, Nobutoshi Yamaguchi, Atsushi J. Nagano, Ayato Sato, Makoto Shirakawa, Toshiro Ito

“…Treatment with DVRs reactivated the expression of FLOWERING LOCUS C (FLC), a master repressor of flowering, by reducing the deposition of repressive histone modifications, thereby delaying flowering time. …”
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Glycosylated lysosomal membrane protein promotes tissue repair after spinal cord injury by reducing iron deposition and ferroptosis in microglia by Fangru Ouyang, Meige Zheng, Jianjian Li, Jinxin Huang, Jianan Ye, Jingwen Wang, Yuanzhe Zhao, Fangli Shan, Ziyu Li, Shuishen Yu, Fei Yao, Dasheng Tian, Li Cheng, Juehua Jing

“…Concurrently, microglia exhibited elevated expression of the iron-storage protein ferritin and were found to undergo ferroptosis between 7 and 28 dpi. …”
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La formación de consensos en las asociaciones empresarias bajo contextos de crisis by Marina Virginia Dossi

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A Bibliometric Analysis of Medullary Thyroid Carcinoma From 2000 to 2022 by TAN Meijuan, YANG Shijie, XU Xiequn

“…High frequency keywords form four clusters: RET gene expression, prognostic factors, MTC clinical management, and MTC treatment. …”
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High prevalence of p16 staining in malignant tumors. by Noémi De Wispelaere, Sebastian Dwertmann Rico, Marcus Bauer, Andreas M Luebke, Martina Kluth, Franziska Büscheck, Claudia Hube-Magg, Doris Höflmayer, Natalia Gorbokon, Sören Weidemann, Katharina Möller, Christoph Fraune, Christian Bernreuther, Ronald Simon, Christian Kähler, Anne Menz, Andrea Hinsch, Frank Jacobsen, Patrick Lebok, Till Clauditz, Guido Sauter, Ria Uhlig, Waldemar Wilczak, Stefan Steurer, Eike Burandt, Rainer Krech, David Dum, Till Krech, Andreas Marx, Sarah Minner

“…However, the prevalence of p16 expression in different cancer types is controversial. 15,783 samples from 124 different tumor types and 76 different normal tissue types were analyzed by immunohistochemistry in a tissue microarray format. p16 was detectable in 5,292 (45.0%) of 11,759 interpretable tumors. …”
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Unraveling the Beneficial Role of Resveratrol in Fructose-Induced Non-Alcoholic Steatohepatitis with a Focus on the AMPK/Nrf2 Signaling Axis by Soha S. Zakaria, Safaa M. Hanafy

“…Quantitative real-time PCR was used to assess the mRNA expression of nuclear factor E2-related factor 2 (Nrf2) in the liver samples. …”
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Study on Mechanical Properties of Gravelly Sand under Different Stress Paths by Dongjie Zhang, Fei Luo, Zhanyuan Zhu, Jiaming Liu, Jing Li, Bailin Li, Tianlang Xue

“…Subsequently, the relationship expression of stress ratio η (q/p) and void ratio e was established, and the trend of void ratio e with the stress path was studied. …”
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Reversal of Early Diabetic Nephropathy by Islet Transplantation under the Kidney Capsule in a Rat Model by Yunqiang He, Ziqiang Xu, Mingshi Zhou, Minmin Wu, Xuehai Chen, Silu Wang, Kaiyan Qiu, Yong Cai, Hongxing Fu, Bicheng Chen, Mengtao Zhou

“…The urine microalbumin-to-creatinine ratio (ACR), protein-to-creatinine ratio, and mean thickness of the glomerular basement membrane (GBM) were significantly decreased in the IT group. The expression of renal fibrotic factors was also significantly decreased. …”
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Single Nucleotide Polymorphisms in PPARD Associated with Systemic Lupus Erythematosus in Chinese Populations by Yuan-yuan Qi, Ya-ling Zhai, Xin-ran Liu, Xiao-xue Zhang, Ya-fei Zhao, Xiang-hui Ning, Zhan-Zheng Zhao

“…Moreover, individuals with the risk C allele were associated with lower expression of PPARD and DEF6. Our clinical analysis showed that SLE patients with the risk C allele of rs4713853 were more likely to present a higher proportion of anti-Sm antibody presence (CC+CT vs. …”
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Daclatasvir and Sofosbuvir Therapy Enhance Monocyte Phenotypic Changes in Naive Chronic Hepatitis C Patients: A Prospective Cohort Study by Hanan M. Fayed, Ali A. Ghweil, Mona M. AbdelMeguid

“…A longitudinal study involving 100 treatment-naïve patients and 30 healthy controls, tested for liver function, fibrosis scores (AST to platelet ratio index, FIB-4), and blood monocyte subsets based on CD14/CD16 expression by flow cytometer. Results. CHC patients had significantly lower albumin, higher ALT, AST, alkaline phosphatase, and increased fibrosis scores [Fib-4 (1.85±0.98) and AST to platelet ratio index (APRI) (0.6±0.35)], higher monocyte and eosinophil counts and lowered neutrophil to monocyte ratio (NMR), and lymphocyte to monocyte ratio (LMR) compared to week 12 and control. …”
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Neural Plasticity Associated with Hippocampal PKA-CREB and NMDA Signaling Is Involved in the Antidepressant Effect of Repeated Low Dose of Yueju Pill on Chronic Mouse Model of Lear... by Zhilu Zou, Yin Chen, Qinqin Shen, Xiaoyan Guo, Yuxuan Zhang, Gang Chen

“…The chronic LH upregulated the expression of NMDA receptor subunits NR1, NR2A, and NR2B, which were all attenuated by Yueju. …”
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Normal tissue radioprotection by amifostine via Warburg-type effects by Michael I. Koukourakis, Alexandra Giatromanolaki, Christos E. Zois, Dimitra Kalamida, Stamatia Pouliliou, Ilias V. Karagounis, Tzu-Lan Yeh, Martine I. Abboud, Timothy D. W. Claridge, Christopher J. Schofield, Efthimios Sivridis, Costantinos Simopoulos, Savvas P. Tokmakidis, Adrian L. Harris

“…Although amifostine in vitro did not inhibit the activity of the prolyl-hydroxylase PHD2, experiments with mouse liver showed that on a short timescale WR-1065 induced expression of the Hypoxia Inducible Factor HIF1α, lactate dehydrogenase LDH5, glucose transporter GLUT2, phosphorylated pyruvate dehydrogenase pPDH and PDH-kinase. …”
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Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE2 Production by Suppression of the AP-1/p38 Pathway by Woo Seok Yang, Yung Chul Park, Ji Hye Kim, Hye Ri Kim, Tao Yu, Se Eun Byeon, Larry D. Unsworth, Jaehwi Lee, Jae Youl Cho

“…Similarly, there was inhibition of cyclooxygenase- (COX-) 2 mRNA expression assessed by real-time PCR, indicating that the inhibition is at the transcriptional level. …”
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Mechanisms of Cytotoxicity of Chemical Agents to Giant Cell Tumors: An In Vitro Study by Achmad Fauzi Kamal, Akbar Rizki Beni Asdi, Ahmad Jabir Rahyussalim, Rio Wikanjaya, Resda Akhra Syahrani, Tri Kurniawati, Septelia Inawati Wanandi

“…Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, were directly administered, in vitro, with concentrations of 1%, 3%, and 5% of H2O2 and 75%, 85%, and 95% of ethanol for 10 minutes and concentrations of 0.003%, 0.005%, 0.01%, 0.03%, 0.1%, and 0.3% of H2O2 for 5 minutes and were incubated for 24 hours. …”
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