New Insights into Behçet’s Syndrome Metabolic Reprogramming: Citrate Pathway Dysregulation by Anna Santarsiero, Pietro Leccese, Paolo Convertini, Angela Padula, Paolo Abriola, Salvatore D’Angelo, Faustino Bisaccia, Vittoria Infantino

“…The aim of our study was to evaluate SLC25A1 and ACLY expression levels in BS patients. Following a power analysis undertaken on few random samples, the number of enrolled patients was set. …”
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Faecalibacterium longum alleviates high-fat diet-induced obesity and protects the intestinal epithelial barrier in mice by Haoyu Wang, Shengnan Yu, Kunyi Zhao, Tongyuan Hu, Zhinan Wu, Hewei Liang, Xiaoqian Lin, Luwen Cui, Jingtong Yao, Xiaomin Liu, Xin Tong, Ningning He, Liang Xiao, Karsten Kristiansen, Shangyong Li, Yuanqiang Zou

“…F. longum CM04–06 protected the intestinal epithelial barrier increasing the expression of tight junction proteins. Metagenomic sequencing indicated that F. longum supplementation did not change the overall composition of the gut microbiota in mice, but selectively increased the relative abundance of Staphylococcus xylosus and Staphylococcus nepalensis. …”
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Possible Involvement of a Mitochondrial Translation Initiation Factor 3 Variant Causing Decreased mRNA Levels in Parkinson's Disease by Anna Anvret, Caroline Ran, Marie Westerlund, Ann-Christin Thelander, Olof Sydow, Charlotta Lind, Anna Håkansson, Hans Nissbrandt, Dagmar Galter, Andrea Carmine Belin

“…We also demonstrated that the TT-genotype causes a significant decrease in MTIF3 mRNA expression compared to the CC-genotype (P=.0163). Our findings support the hypothesis that MTIF3 may be involved in the etiology of PD.…”
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Exendin-4 Protects MIN6 Cells from t-BHP-Induced Apoptosis via IRE1-JNK-Caspase-3 Signaling by Wen-Jia Chen, Lin-Xi Wang, Yan-Ping Wang, Zhou Chen, Xiao-Ying Liu, Xiao-Hong Liu, Li-Bin Liu

“…The activity of caspase-3 was determined using a caspase-3 activity kit. Expression of P-IRE1α, IRE1α, C-Jun N-terminal kinase (JNK), P-JNK, C-JUN, and P-C-JUN was detected by western blotting. …”
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Differences in Transcriptional Activation by the Two Allelic (L162V Polymorphic) Variants of PPARα after Omega-3 Fatty Acids Treatment by Iwona Rudkowska, Mélanie Verreault, Olivier Barbier, Marie-Claude Vohl

“…Omega-3 fatty acids (FAs) have the potential to regulate gene expression via the peroxisome proliferator-activated receptor α (PPARα); therefore, genetic variations in this gene may impact its transcriptional activity on target genes. …”
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Uptake of iron from ferrous fumarate can be mediated by clathrin-dependent endocytosis in Hutu-80 cells by Agata Tarczykowska, Per Malmberg, Nathalie Scheers

“…We used siRNA to silence DMT1 transporter expression, pharmacological inhibition of endocytosis, and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) to show that iron uptake from ferrous fumarate can be mediated by both transport via DMT1 and by clathrin-dependent endocytosis in Hutu-80 cells. …”
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Therapeutic implications and comprehensive insights into cellular senescence and aging in the tumor microenvironment of sarcoma by Pengfei Zan, Yi Zhang, Yidong Zhu, Qingjing Chen, Zhengwei Duan, Yonghao Guan, Kaiyuan Liu, Anquan Shang, Zihua Li

“…Utilizing the weighted gene co-expression network analysis and least absolute shrinkage and selection operator algorithm, we identified senescence-related genes and developed a senescence predictor. …”
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Curcumin Attenuation of Wear Particle-Induced Osteolysis via RANKL Signaling Pathway Suppression in Mouse Calvarial Model by Tao Cheng, Yaochao Zhao, Bin Li, Mengqi Cheng, Jiaxing Wang, Xianlong Zhang

“…Furthermore, CUR downregulated the osteoclast-specific gene expression and reversed the receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin messenger RNA and protein ratio in PMMA particle-stimulated mice. …”
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A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection. by Emily A Fitzmeyer, Taru S Dutt, Silvain Pinaud, Barb Graham, Emily N Gallichotte, Jessica L Hill, Corey L Campbell, Hunter Ogg, Virginia Howick, Mara K N Lawniczak, Erin Osborne Nishimura, Sarah Hélène Merkling, Marcela Henao-Tamayo, Gregory D Ebel

“…Rather, we observed a significant positive correlation between immune gene expression levels and vRNA load in individual cells, suggesting that within midgut cells, high levels of vRNA may trigger antiviral responses. …”
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Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells by Niina Hopper, John Wardale, Daniel Howard, Roger Brooks, Neil Rushton, Frances Henson

“…Cell migration was measured using an xCELLigence electronic migration chamber system in combination with scratch assays. Gene expression was quantified with stem cell PCR arrays and validated using quantitative real-time PCR (rtPCR). …”
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Mice with NOP2/sun RNA methyltransferase 5 deficiency die before reaching puberty due to fatal kidney damage by Hongya Zhang, Xiaohui Li, Jing Bai, Cong Zhang

“…Furthermore, we found that the kidneys of Nsun5-/- mice showed increased expression of the apoptotic signal Caspase-3 and accumulated stronger DNA damage at PD 21.Conclusions In our study, we found that mice lacking NSUN5 died before puberty due to kidney fatal damage caused by DNA damage and cell apoptosis. …”
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Structural and Functional Characterization of Recombinant Interleukin-10 from Indian Major Carp Labeo rohita by Sweta Karan, Pujarini Dash, Himani Kaushik, Pramoda K. Sahoo, Lalit C. Garg, Aparna Dixit

“…In the present study, we report large scale production and purification of biologically active recombinant IL-10 of L. rohita (rLrIL-10) using a heterologous expression system and its biophysical and functional characterization. …”
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Male infertility along with the era of coronavirus infection SARS-CoV-2 by N. G. Kulchenko, N. K. Druzhinina, G. I. Myandina

“…It is known that due to the high expression of ACE2 in spermatogonia, Leydig and Sertoli cells, the testicle, as an organ producing male germ cells, is a potential target for the COVID‑19 virus, which directly affects the reproductive health of men. …”
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Role of sirtuins in epigenetic regulation and aging control by E. M. Samoilova, S. E. Romanov, D.  A. Chudakova, P. P. Laktionov

“…However, the SIRT influence on the epigenetic regulation of gene expression during the aging process and the development of disorders is one of the most important aspects of maintaining the homeostasis of organs and tissues. …”
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ANÁLISE ERGONÔMICA PRELIMINAR DE UMA MICROEMPRESA DE MANUFATURA DE CABOS NÁUTICOS by Bárbara Victória Silva Lopes, Jonas Borges Pantaleão, Italo Rodeghiero Neto, Lucas Gomes Miranda Bispo, Fernando Gonçalves Amaral

“…Para cada rubrica analisada e discutida, as expressões faciais resultantes foram definidas em consenso. …”
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Disomic chromosome 3R(3B) substitution causes a complex of meiotic abnormalities in bread wheat <i>Triticum aestivum</i> L. by А. А. Zhuravleva, О. G. Silkova

“…Triticum aestivum L. lines introgressed with alien chromosomes create a new genetic background that changes the gene expression of both wheat and donor chromosomes. The genes involved in meiosis regulation are localized on wheat chromosome 3B. …”
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Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs by Ming-Xia Duan, Heng Zhou, Qing-Qing Wu, Chen Liu, Yang Xiao, Wei Deng, Qi-Zhu Tang

“…Andr ameliorated the levels of phosphorylated PI3K (p-PI3K), phosphorylated AKT (p-AKT), and phosphorylated eNOS (p-eNOS). The expression of vascular endothelial growth factor (VEGF) protein, a vital factor in angiogenesis, was improved by Andr treatment under HG stimulation. …”
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The ubiquitin-conjugating enzyme UBE2D maintains a youthful proteome and ensures protein quality control during aging by sustaining proteasome activity. by Liam C Hunt, Michelle Curley, Kudzai Nyamkondiwa, Anna Stephan, Jianqin Jiao, Kanisha Kavdia, Vishwajeeth R Pagala, Junmin Peng, Fabio Demontis

“…Mechanistically, UBE2D/eff is necessary to maintain optimal proteasome function: UBE2D/eff knockdown reduces the proteolytic activity of the proteasome, and this is rescued by transgenic expression of human UBE2D2, an eff homolog. Likewise, human UBE2D2 partially rescues the lifespan and proteostasis deficits caused by muscle-specific effRNAi and re-establishes the physiological levels of effRNAi-regulated proteins. …”
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COVID-19-Related Mental Health Burdens: Impact of Educational Level and Relationship Status Among Low-Income Earners of Western Uganda by Lemuel, Ann Monima, Usman, Ibe Michael, Keneth Iceland, Kasozi, Alghamdi, Saad, Aigbogun, Eric Osamudiamwen, Archibong, Victor, Ssebuufu, Robinson, Kabanyoro, Annet, Ifie, Josiah Eseoghene, Swase, Dominic Terkimbi, Ssempijja, Fred, Ayuba, John Tabakwot, Matama, Kevin, Onohuean, Hope, Kembabazi, Stellamaris, Henry, Rachael, Odoma, Said, Yusuf, Helen, Afodun, Adam Moyosore, Assaggaf, Hamza M., Kairania, Emmanuel, Aslam, Akhmed, Okon, Owoisinke, Batiha, Gaber El-Saber, Welburn, Susan Christina

“…Anxiety, anger, and depression were assessed using a modified generalized anxiety disorder (GAD-7), Spielberger’s State-Trait Anger Expression Inventory-2, and Beck Depression Inventory item tools, respectively. …”
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Assessment of Bowel Preparation Using Low-Volume Sulphate-Based Preparations in Comparison with Macrogols: A Multicenter, Randomized, Comparative Clinical Study of the 3rd Phase by E. D. Fedorov, V. V. Veselov, S. V. Kashin, E. V. Tikhomirova, A. V. Veselov, D. V. Zavyalov, A. Kornowski, T. E. Gorskaya, M. Volteau, T. Ponchon

“…The authors also express their appreciation to Olga Kapitonova, an employee of the Almedis company (Moscow, Russia) for her assistance in compiling medical texts, which activity was carried out under the financial support of the Ipsen company (Moscow, Russia) in accordance with the Good Publication Practice (GPP).…”
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