Defects of CTLA-4 Are Associated with Regulatory T Cells in Myasthenia Gravis Implicated by Intravenous Immunoglobulin Therapy by Wenhua Xu, Mingshan Ren, Swagata Ghosh, Kai Qian, Zhaofeng Luo, Aimei Zhang, Cuiping Zhang, Jiajun Cui

“…In an effort to examine the roles of various subsets of CD4+ T cells in the periphery blood of MG and uncover the mechanisms that contribute to the therapeutical effects of IVIg, we first demonstrated that a subset of CD4+ T cells, CTLA-4-expressing regulatory T (Treg) cells, were underrepresented and functionally defective in MG patients. …”
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EFFECT OF FLANKING SEQUENCES ON THE ACCURACY OF THE RECOGNITION OF TRANSCRIPTION FACTOR BINDING SITES by T. M. Khlebodarova, D. Yu. Oshchepkov, V. G. Levitsky, O. A. Podkolodnaya, E. V. Ignatieva, E. A. Ananko, I. L. Stepanenko, N. A. Kolchanov

“…The development of in vitro methods produced new experimental information on protein binding to DNA, which is accumulated in databases and used in studies of mechanisms regulating gene expression and in the development of computer-assisted methods of binding site recognition in pro- and eukaryotic genomes. …”
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Early-life gut mycobiome core species modulate metabolic health in mice by Mackenzie W. Gutierrez, Erik van Tilburg Bernardes, Ellen Ren, Kristen N. Kalbfleisch, Madeline Day, Ewandson Luiz Lameu, Thaís Glatthardt, Emily M. Mercer, Sunita Sharma, Hong Zhang, Ali Al-Azawy, Faye Chleilat, Simon A. Hirota, Raylene A. Reimer, Marie-Claire Arrieta

“…Intestinal nutrient transporter expression was unaffected by the presence of fungi in jejunal enteroids, yet the immune landscape in white adipose tissue was distinctly impacted by each fungal species, suggesting that these phenotypes may be a result of fungal immune regulation. …”
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Design and Mechanism Study of 6c, a Novel Artesunate Derivatives, for Anti-Hepatocellular Carcinoma by Xiong SS

“…Western blot was used to observe the expression of apoptosis-related proteins. The ability of 6c to induce apoptosis was detected by Annexin V-FITC/PI double staining and Hoechst 33342 staining experiment. …”
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Fracture Mechanical Behavior of Cracked Cantilever Roof with Large Cutting Height Mining by Zenghui Zhao, Wei Sun, Mingzhong Zhang, Xiaojie Gao, Shaojie Chen

“…The roof was divided into two parts: the crack-affected area and the crack-unaffected area. The analytical expression of the boundary between the two areas was established by fracture mechanics methods. …”
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Modes-of-Action Related to Repeated Dose Toxicity: Tissue-Specific Biological Roles of PPARγ Ligand-Dependent Dysregulation in Nonalcoholic Fatty Liver Disease by Merilin Al Sharif, Petko Alov, Vessela Vitcheva, Ilza Pajeva, Ivanka Tsakovska

“…A detailed analysis was performed regarding proteins which PPARγ-mediated expression changes had been confirmed to be prosteatotic by most experimental evidence. …”
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A A EDUCAÇÃO EM CIÊNCIA, TECNOLOGIA E SOCIEDADE NO PROJETO POLÍTICO PEDAGÓGICO DE UMA ESCOLA DA REDE MUNICIPAL DE CURITIBA by Camila Renata Texeira de Souza da Silva Souza, Leonir Lorenzetti, Tamara Simone van Kaick

“…A verificação dos alinhamentos se deu por meio de uma análise subjetiva que puderam ser expressos qualitativamente e em alguns momentos traduzidos de forma quantitativa. …”
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Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences by Jianfeng Sun, Martin Philpott, Danson Loi, Gabriela Hoffman, Jonathan Robson, Neelam Mehta, Eleanor Calcutt, Vicki Gamble, Tom Brown, Tom Brown, Udo Oppermann, Adam P. Cribbs

“…Our results demonstrate that this anchor-enhanced design substantially improves gene expression profiles in droplet-based single-cell sequencing analyses.…”
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Melanophilin-induced primary cilia promote pancreatic cancer metastasis by Yu-Ying Chao, Ruei-Ci Lin, Ping-Jui Su, Chu-An Wang, Ting-Yuan Tu, Ya-Chin Hou, Yi-Tzui Tsai, I-Chen Peng, Shaw-Jenq Tsai, Yan-Shen Shan, Chia-Yih Wang

“…Clinically, high MLPH expression is positively correlated with metastasis and poor PDAC prognosis. …”
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Life-history adaptation under climate warming magnifies the agricultural footprint of a cosmopolitan insect pest by Estelle Burc, Camille Girard-Tercieux, Moa Metz, Elise Cazaux, Julian Baur, Mareike Koppik, Alexandre Rêgo, Alex F Hart, David Berger

“…We find support for these predictions by studying thermal adaptation in life-history traits and gene expression in the wide-spread insect pest, Callosobruchus maculatus; with 5 years of evolution under experimental warming causing an almost two-fold increase in its predicted agricultural footprint. …”
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Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose by Can Wu, Ningning Qin, Huiwen Ren, Min Yang, Shuang Liu, Qiuyue Wang

“…To clarify the function of Egr1 on the inflammation and fibrosis in high glucose-cultured MCs, as well as to explore the effects of metformin on miR-34a pathway and Egr1 expression. Methods. We transfected MCs with miR-34a inhibitor. …”
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Autoimmunity against cytokines: Double strike in autoimmune disease, a historical perspective by Iván Insignares, Luis E. Rodríguez, Óscar Correa-Jiménez, Alberto Alfaro-Murillo, Laura Rincón-Arenas, Andrés Sánchez, Marlon Múnera

“…This leads to an effector immune response against tissues expressing antigens, which are later recognized by the host immune system. …”
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Transcranial Direct Current Stimulation Modulates Neurogenesis and Microglia Activation in the Mouse Brain by Anton Pikhovych, Nina Paloma Stolberg, Lea Jessica Flitsch, Helene Luise Walter, Rudolf Graf, Gereon Rudolf Fink, Michael Schroeter, Maria Adele Rueger

“…Multisession tDCS at a sublesional charge density led to a polarity-dependent downregulation of the constitutive expression of Iba1 by microglia in the mouse cortex. …”
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Metformin delays the decline in thermogenic function of brown adipose tissue in a mouse model of Hutchinson-Gilford progeria syndrome by Xin Xiang, Yuyue Feng, Hongcheng Li, Wenbo Li, Jia Li, Zhu Xia, Hua Pang, Zhengjie Wang

“…Additionally, metformin slowed the age-related decline in UCP1 expression levels in brown adipose tissue, thereby maintaining the thermogenic capacity of the progeroid mice. …”
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Imaging lipid rafts reveals the principle of ApoE4-induced Aβ upregulation in human neurons by Se-In Lee, Heejin Lim, Na Yeon Kim, Jichang Yu, Joonho Cho, Hyein Lee, Dae Won Moon, Jinsoo Seo

“…Summary: Lipid rafts in plasma membranes are thought to provide a platform for regulating signaling pathways by increasing the expression or proximity of proteins in the same pathway. …”
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Insights into the toxic impact of long-term exposure to diethyl phthalate on commercially important species Catla (Labeo catla) by Satabdi Ganguly, Basanta Kumar Das, Anupam Adhikari, Subir Kumar Nag

“…Results DEP exposure to 1/10th (1.62 mg/L) and 1/50th (0.32 mg/L) LC50 dose for 30 days to Catla revealed that it stimulated the expression of kisspeptin (Kiss 1 and Kiss 2) genes in the hypothalamus, leading to an increased GnRH concentration (36.75%) in the higher dose. …”
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Korelasi Kadar Adiponektin dengan Kadar Kolesterol High Density Lipoprotein pada Penyandang Obes by Fiona Septi Mulya, Efrida Efrida, Zelly Dia Rofinda

“…This causes ATP-binding cassette transporter A1 expression and apolipoprotein A-I synthesis in hepatocytes to decrease, resulting in impaired HDL cholesterol synthesis. …”
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Comprehensive landscape and oncogenic role of extrachromosomal circular DNA in malignant biliary strictures by Zhuo Cheng, Xuanmei Luo, Wenzheng Liu, Xiaofang Lu, Hong Chang, Yingchun Wang, Wei Zheng, Xiue Yan, Yonghui Huang

“…EccDNA carrying miR-106a/363 cluster or miR-374b/421 cluster were proven to regulate cancer gene expression, accelerate tumor proliferation, and inhibit tumor apoptosis. …”
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Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway by Chenlong Wang, Chuangjiang Chen, MengYa Wang, Sajid Ur Rahman, Bin Wei, Hongyan Ding, Wanyue Huang, Xichun Wang

“…Additionally Rutin lowered the expression of p53, ALOX12, and ACSL4 while significantly improving cytokinesis, antioxidant enzyme activity, and SLC7A11, GPX4, Nrf2, FTH1, thereby inhibiting cellular ferroptosis. …”
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Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines by Elizabeth Curtiss, Youde Jiang, Li Liu, Claire Hawthorne, Jessica Zhang, Jena J. Steinle

“…Primary human REC were cultured in normal and high glucose followed by Epac1 agonist treatment or transfection with IL-1β or TNFα siRNA for protein analyses of insulin signaling proteins. Decreased expression of the insulin receptor was observed in the Epac1 knockout mouse retinal vasculature compared to floxed littermates. …”
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