Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates by Ligiane de Oliveira Leme, Mauricio Machaim Franco, Otávio Augusto de Faria, Alexandre Rodrigues Caetano, Juliana Gonçalves de Souza, Luciano de Rezende Carvalheira, Emivaldo de Siqueira Filho, Margot Alves Nunes Dode

“…First, embryos produced under these three treatments were evaluated for production, quality, lipid content, gene expression, and methylation patterns. The results indicated that all parameters analyzed were similar across all treatments (P > 0.05), suggesting that reducing media components does not affect embryo development and quality. …”
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Generation of two induced pluripotent stem cell lines to model and investigate diseases affecting Pacific Islanders by Angela Zhang, James W.S. Jahng, Julio V. Guevara, Christopher D. Yan, Michael V. McConnell, Joseph C. Wu

“…We demonstrate that the lines exhibit normal morphology and karyotypes, robust expression of pluripotent markers, and the capacity for trilineage differentiation.…”
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Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk by A. Yu. Novoselskaya-Dragovich, A. A. Yankovskaya, E. D. Badaeva

“…The absence of alien genes expression in the lines carrying a long arm deletion in chromosome 6Xc suggested that the gliadin-coding locus moved from the short chromosome arm (its characteristic position in all known wheat species) to the long one. …”
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dotears: Scalable and consistent directed acyclic graph estimation using observational and interventional data by Albert Xue, Jingyou Rao, Sriram Sankararaman, Harold Pimentel

“…In real data, differential expression tests and high-confidence protein-protein interactions validate dotears-inferred edges with higher precision and recall than others.…”
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Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells by Shuang Liu, Minae Takahashi, Takeshi Kiyoi, Kensuke Toyama, Masaki Mogi

“…Upregulation of intracellular Ca2+ signals attenuated the adipogenic differentiation ability and slightly increased the osteogenic differentiation potency of MSCs, whereas downregulation of CRACM1 expression promoted chondrogenic differentiation potency. …”
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Bioinformatic Prediction of Novel microRNAs Encoded in Krüppel-like Factor 4 Gene by Mina Zahiri, Maryam Hassanlou, Amir-Reza Javanmard, Nooshin Bijari

“…MicroRNAs are small non-coding RNAs that can regulate gene expression that affects various cellular processes. …”
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Unravelling a mechanistic link between mitophagy defect, mitochondrial malfunction, and apoptotic neurodegeneration in Mucopolysaccharidosis VII by Nishan Mandal, Apurba Das, Rupak Datta

“…This was evidenced by lower Atg8a-II levels, reduced Atg1 and Ref(2)P expression along with accumulation of lipofuscin-like inclusions and multilamellar bodies. …”
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Multi-Server Two-Way Communication Retrial Queue Subject to Disaster and Synchronous Working Vacation by Tzu-Hsin Liu, He-Yao Hsu, Fu-Min Chang

“…For this model, we derive an explicit expression for the stationary distribution with the help of the quasi-birth-and-death process and the matrix geometric method. …”
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Report of a Novel Mutation in MLH1 Gene in a Hispanic Family from Puerto Rico Fulfilling Classic Amsterdam Criteria for Lynch Syndrome by Juan M. Marqués-Lespier, Yaritza Diaz-Algorri, Maria Gonzalez-Pons, Marcia Cruz-Correa

“…Our proband was identified by applying Amsterdam and Bethesda criteria for Lynch syndrome, analysis of protein expression by immunohistochemistry, and genetic sequencing of the mismatch repair genes. …”
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12,13-diHOME Promotes Inflammatory Macrophages and Epigenetically Modifies Their Capacity to Respond to Microbes and Allergens by Din L. Lin, Kevin M. Magnaye, Cara E. Porsche, Sophia R. Levan, Elze Rackaityte, Mustafa Özçam, Susan V. Lynch

“…Exposure to 12,13-diHOME also induces macrophage chromatin remodeling, specifically diminishing access to interferon-stimulated response elements resulting in reduced interferon-regulated gene expression upon bacterial lipopolysaccharide stimulation. …”
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Flavonoids as Strong Inhibitors of MAPK3: A Computational Drug Discovery Approach by Amir Taherkhani, Parita Khodadadi, Lida Samie, Zahra Azadian, Zeynab Bayat

“…Negative regulation of MAPK3 expression using miRNAs has led to therapeutic effects in cancer. …”
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A quality control method based on physical constraints and data-driven collaborative artificial intelligence for wind observations along high-speed railway lines by X. Xiong, J. Chen, Y. Zhang, X. Chen, Y. Zhang, X. Ye

“…On the other hand, to solve the constrained error mapping expression under different wind conditions, a data-driven model of hyperparameter optimization (BO-XGBoost) is introduced to perform error compensation on physical relationships. …”
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Potential Role of Peptidylarginine Deiminase Enzymes and Protein Citrullination in Cancer Pathogenesis by Sunish Mohanan, Brian D. Cherrington, Sachi Horibata, John L. McElwee, Paul R. Thompson, Scott A. Coonrod

“…At the molecular level, transcription factors, coregulators, and histones are functional targets for citrullination by PADs, and citrullination of these targets can affect gene expression in multiple tumor cell lines. Next generation isozyme-specific PAD inhibitors may have therapeutic potential to regulate both the inflammatory tumor microenvironment and tumor cell growth.…”
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Interleukin-22 Might Act as a Double-Edged Sword in Type 2 Diabetes and Coronary Artery Disease by Fangchen Gong, Jin Wu, Ping Zhou, Mengyao Zhang, Jingning Liu, Ying Liu, Xiang Lu, Zhengxia Liu

“…Conversely, IL-22 was found to protect endothelial cells from glucose- and lysophosphatidylcholine- (LPC-) induced injury, and IL-22R1 expression on endothelial cells was increased upon treatment with high glucose and LPC. …”
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Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease by Alfredo Smiriglia, Nicla Lorito, Marina Bacci, Angela Subbiani, Francesca Bonechi, Giuseppina Comito, Marta Anna Kowalik, Andrea Perra, Andrea Morandi

“…These exposures led to lipid droplet (LD) accumulation, increased reactive oxygen species (ROS) levels, and mitochondrial dysfunction, along with decreased expression of markers associated with hepatocyte functionality and differentiation. …”
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Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts by Corey Heffernan, Huseyin Sumer, Luis F. Malaver-Ortega, Paul J. Verma

“…Exogenous expression of Oct4, Sox2, Klf4, and cMyc forces mammalian somatic cells to adopt molecular and phenotypic characteristics of embryonic stem cells, commencing with the required suppression of lineage-associated genes (e.g., Thy1 in mouse). …”
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Morphofunctional variations of the Echinometra lucunter (Echinoidea) on Bahia Coast, Brazil by Walter Ramos Pinto Cerqueira, Yara Aparecida Garcia Tavares, Natalie Petrovna Semanovschi, Daniele Nascimento de Jesus

“…The environments with distinct characteristics and wide food availability associated with a broad spectrum of the trophic niche occupied by the populations point out modulations and specializations of morphofunctional strategies and the expression of phenotypes.…”
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Renal Protection by Genetic Deletion of the Atypical Chemokine Receptor ACKR2 in Diabetic OVE Mice by Shirong Zheng, Susan Coventry, Lu Cai, David W. Powell, Venkatakrishna R. Jala, Bodduluri Haribabu, Paul N. Epstein

“…Activation of pathways that increase inflammatory gene expression was attenuated. Human biopsies stained with ACKR2 antibody revealed increased staining in diabetic kidney, especially in some tubule and interstitial cells. …”
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Non-Canonical, Extralysosomal Activities of Lysosomal Peptidases in Physiological and Pathological Conditions: New Clinical Opportunities for Cancer Therapy by Ryan Conesa-Bakkali, Macarena Morillo-Huesca, Jonathan Martínez-Fábregas

“…Under physiological conditions, lysosomal proteases, through non-canonical, extralysosomal activities, have been linked to cell differentiation, regulation of gene expression, and cell division. Under pathological conditions, these proteases have been linked to cancer, mostly through their extralysosomal activities in the cytosol and nuclei of cells. …”
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Interactions between Neutrophils, Th17 Cells, and Chemokines during the Initiation of Experimental Model of Multiple Sclerosis by Dagmara Weronika Wojkowska, Piotr Szpakowski, Dominika Ksiazek-Winiarek, Marcin Leszczynski, Andrzej Glabinski

“…Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) in which activated T cell and neutrophil interactions lead to neuroinflammation. In this study the expression of CCR6, CXCR2, and CXCR6 in Th17 cells and neutrophils migrating to the brain during EAE was measured, alongside an evaluation of the production of IL-17, IL-23, CCL-20, and CXCL16 in the brain. …”
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