Immune regulatory genes impact the hot/cold tumor microenvironment, affecting cancer treatment and patient outcomes by Mengmeng Sang, Jia Ge, Juan Ge, Juan Ge, Gu Tang, Qiwen Wang, Jiarun Wu, Liming Mao, Liming Mao, Xiaoling Ding, Xiaorong Zhou

“…Additionally, hub genes that regulate the tumor microenvironment were identified, and their expression patterns were analyzed using single-cell RNA sequencing. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Methods We employed microarray and single-cell RNA sequencing (scRNA-seq) to investigate gene expression changes and immune cell dynamics in mice following crush injury at 3 and 7 days post-injury (dpi). …”
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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…To design plasmid vectors for efficient production of the recombinant monoclonal antibody FM1 in a eukaryotic expression system targeting the RSV fusion (F) glycoprotein and to evaluate its activity against RSV subtypes A and B in vitro. …”
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Drosophila ubiquitin-specific peptidase 14 stabilizes the PERIOD protein by regulating a ubiquitin ligase SLIMB by So Who Kang, Jung-Eun Park, Soonhyuck Ok, Minhui Um, Hyeonjeong Son, Seunghee Byun, Nayoung Park, Su Jin Lee, Thị Xuân Thùy Trần, Gyeongmin Kim, Jeonghun Yeom, Kyunggon Kim, Eun Young Kim, Min-Ji Kang

“…Moreover, reducing slimb expression mitigates the effects of USP14 on PER protein stability. …”
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Updated Bayesian network meta-analysis on the efficacy and safety of PD−1 versus PD−L1 inhibitors in first−line treatment with chemotherapy for extensive−stage small-cell lung canc... by Ke Wang, Ke Wang, Chuangjie Zheng, Chuangjie Zheng, Xinrong Chen, Xinrong Chen, Penghui Lin, Mengge Lin, Cuizhen Chen, Linzhu Zhai

“…Patients with PD-L1 expression levels < 1%, PD-1 + Chemo showed a trend of disadvantage (OS: HR 1.3; PFS: HR 1.2), whereas for patients with PD-L1 expression levels ≥ 1%, PD-1 + Chemo showed a trend of advantage (OS: HR 0.85; PFS: HR 0.85).ConclusionsPD-1 + Chemo and PD-L1 + Chemo significantly prolonged OS and PFS in patients with ES-SCLC and did not significantly increase the incidence of grade ≥ 3 TRAES. …”
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Glycine rich proteins of ticks: more than a cement component by Renata Perotto de Souza, Mariana Vieira Dalla Valentina, Bruna Ferreira Leal, Sílvia Dias Oliveira, Carlos Alexandre Sanchez Ferreira, Ala Tabor

“…Conversely, several GRPs are present in all tick developmental stages, and the expression of many GRP genes is modulated by physiological processes and immune challenges, such as feeding and pathogen infection. …”
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Pirfenidone Accelerates Wound Healing in Chronic Diabetic Foot Ulcers: A Randomized, Double-Blind Controlled Trial by Luz E. Gasca-Lozano, Silvia Lucano-Landeros, Héctor Ruiz-Mercado, Adriana Salazar-Montes, Ana Sandoval-Rodríguez, Jesus Garcia-Bañuelos, Arturo Santos-Garcia, Judith R. Davila-Rodriguez, José Navarro-Partida, Hiram Bojórquez-Sepúlveda, Juan Castañeda-Gomez, José Domínguez-Rosales, Myriam A. Ruiz-Arcos, María Guadalupe Sánchez-Parada, Juan Armendariz-Borunda

“…Relative ulcer volume (RUV) was measured every month; biopsies were taken at baseline and months 1 and 2 for histopathology and gene expression analysis for COL-1α, COL-4, KGF, VEGF, ACTA2 (α-SMA), elastin, fibronectin, TGF-β1, TGF-β3, HIF-1α, and HIF-1β. …”
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Protein identification using Cryo-EM and artificial intelligence guides improved sample purification by Kenneth D. Carr, Dane Evan D. Zambrano, Connor Weidle, Alex Goodson, Helen E. Eisenach, Harley Pyles, Alexis Courbet, Neil P. King, Andrew J. Borst

“…This identification was validated through comparisons with DLST structures in the Protein Data Bank, AlphaFold 3 predictions based on the DLST sequence from our E. coli expression vector, and traditional biochemical methods. …”
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Evaluation of the Early Development of 6-Month-Old Babies in the Case of Maternal Postpartum Depression with or Without Bipolar Disorder by Jokthan Guivarch, Mélanie-Lou Persia, Laure Le Treut, Pauline Grandgeorge, Federico Solla, Hugo Pergeline, Michel Dugnat, Florence Askenazy, François Poinso, Arthur Varoquaux, Arnaud Fernandez

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Effects of Lupeol on Intestinal Anastomosis After Experimental Intestinal Ischemia-Reperfusion Injury in Rats by Kaya C, Kapisiz A, Eryilmaz S, Karabulut R, Turkyilmaz Z, Inan MA, Aydin GY, Celik MA, Sonmez K

“…In addition, lupeol decreased miR-34a-5p expression and increased miR-29b-3p and miR-495-3p expression.Conclusion: Lupeol protected the intestines from I/R damage with its antioxidant and anti-inflammatory effects. …”
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Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation by Lais Morandini Rodrigues, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonça, Gustavo Mendonça, Yasmin Rodarte Carvalho, Luana Marotta Reis de Vasconcellos

“…The osteogenic gene Runx2 presented high expression levels for TiNbZrTa at day 7, compared to the control group (TiAlV day 3). …”
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Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model by Ming-Hsien Tsai, How-Ran Chao, Jheng-Jie Jiang, Yu-Hsieh Su, Mariene-syne P. Cortez, Lemmuel L. Tayo, I-Cheng Lu, Hao Hsieh, Chih-Chung Lin, Sheng-Lun Lin, Wan Nurdiyana Wan Mansor, Ching-Kai Su, Sen-Ting Huang, Wen-Li Hsu

“…In the examination of the longevity of nematodes, it was found that the lifespan of all GO NP-exposed worms was significantly shortened as compared to the untreated worms. Gene expression of sod-1, sod-3, and ctl-2 presented significantly higher induction folds in the exposed worms compared with the controls. …”
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CD14loCD301b+ macrophages gathering as a proangiogenic marker in adipose tissues by Yibing Lv, Yidan Zheng, Shanshan Su, Junyi Xiao, Jie Yang, Lingyun Xiong, Yanyan Guo, Xiaoqi Zhou, Nengqiang Guo, Ping Lei

“…Subsequent assays confirmed the enhanced angiogenesis and more accumulation of CD301b+ macrophages in Cd14−/− epididymal adipose tissues. Because Igf1 expression has been suggested to be associated with Cd301b expression through pseudotime analysis, we found it was insulin-like growth factor 1 secreted from Cd14−/− macrophages that mediated the angiogenesis enhancement. …”
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A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice by Hui Wang, Jing Fan, Nan-lin Li, Jun-tang Li, Shi-fang Yuan, Jun Yi, Ling Wang, Jiang-hao Chen, Yong-gang Lv, Qing Yao, Ting Wang, Yu-cai Wang, Rui Ling

“…ISO treatment inhibited iNOS expression and activity, as well as subsequent nitric oxide generation. …”
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Combination Fractional Carbon Dioxide Laser Treatment and Bone Marrow Mesenchymal Stem Cell Therapy Enhances the Treatment of Skin Photoaging in a Murine Model System by Li L, He Z, Yu C, Zhang C, Yu Y, Li Y, Xu X

“…The improvement of effect may be due to the decrease of MMP-3 and MMP-9 expression in combination therapy.Keywords: bone marrow mesenchymal stem cell, fractional carbon dioxide laser, photoaging, matrix metalloproteinase, mouse model…”
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Germinal Center B Cells are Uniquely Targeted by Antibody-Suppressor CXCR5+CD8+ T Cells by Jason M. Zimmerer, PhD, Sachi Chaudhari, BS, Kavya Koneru, BS, Jing L. Han, MD, PhD, Mahmoud Abdel-Rasoul, MA, Hope Uwase, MA, Tai Yi, MD, Christopher K. Breuer, MD, Ginny L. Bumgardner, MD, PhD

“…Alloprimed immune-cell subsets were evaluated for susceptibility to CD8+ TAb-supp cell–mediated in vitro cytotoxicity and/or suppression of intracellular cytokine expression. In vivo CD8-mediated cytotoxicity to wild-type germinal center (GC) B cells or wild-type CD4+ T follicular helper cells (TFH cells) was assessed in RAG1 knockout mice. …”
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Corneal protective effects of a new ophthalmic formulation based on vitamin B12 and sodium hyaluronate by Francesca Lazzara, Federica Conti, Grazia Maugeri, Velia D’Agata, Velia D’Agata, Ludovica Sotera, Claudio Bucolo, Claudio Bucolo

“…Cell viability, cytotoxicity, ROS production, and mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β) were assessed by MTT, LDH, 2′,7′–dichlorofluorescein diacetate (DCFDA) assays and Real-time PCR, respectively. …”
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Cross-Talk between NOK and EGFR: Juxtamembrane and Kinase domain interactions enhancing STAT3/5 signaling in breast cancer tumorigenesis by Yinyin Wang, Bingdong Zhang, Chunhua He, Bo Tian, Sihan Liu, Jianghua Li, Jiayu Wang, Shigao Yang, Bingtao Zhu, Xiaoguang Wang, Zhijie Chang, Chenxi Cao

“…Depletion of NOK impaired, but over-expression of NOK increased, the phosphorylation of EGFR. …”
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The discovery and characterization of K‐563, a novel inhibitor of the Keap1/Nrf2 pathway produced by Streptomyces sp by Ran Hori, Kozo Yamaguchi, Hidetaka Sato, Miwa Watanabe, Kyoko Tsutsumi, Susumu Iwamoto, Masayuki Abe, Hideyuki Onodera, Satoshi Nakamura, Ryuichiro Nakai

“…K‐563 also inhibited the expression of downstream target genes in other Keap1‐ or Nrf2‐mutated cancer cells. …”
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Class III β-Tubulin Overexpression Induces Chemoresistance to Eribulin in a Leiomyosarcoma Cell Line by Kenichiro Yahiro, Yoshihiro Matsumoto, Jun-ichi Fukushi, Ken-ichi Kawaguchi, Makoto Endo, Nokitaka Setsu, Keiichiro IIda, Suguru Fukushima, Makoto Nakagawa, Atsushi Kimura, Yoshinao Oda, Yasuharu Nakashima

“…The resistant cell line showed increased expression of MDR1 transcript, but protein levels and functional analysis results were similar to the parental cell line. …”
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