Molecular-genetic pathways of hepatitis C virus regulation of the expression of cellular factors PREB and PLA2G4C, which play an important role in virus replication by E. L. Mishchenko, A. A. Makarova, E. A. Antropova, A. S. Venzel, T. V. Ivanisenko, P. S. Demenkov, V. A. Ivanisenko

“…Analysis of the gene networks and data on differential gene expression in HCV-infected cells allowed us to hypothesize further how HCV could regulate the expression of TFs, the binding sites of which are localized within PREB and PLA2G4C gene regions. …”
Get full text

Cloning, expression, and purification of fusion antigens MPT83 and ESAT6 from the local strain of Mycobacterium tuberculosis in Escherichia coli as a seed vaccine candidate against tuberculosis by Rusdina Bte Ladju, Ahyar Ahmad, Abdul Karim, Rugaiyah Arfah, Rosana Agus, Najdah Hidayah, Muhammad Nasrum Massi, Astutiati Nurhasanah, Harningsih Karim, Irda Handayani

“…The fusion genes Rv2873+Rv3875 were cloned into the expression vector pTrcHisA. This resulted in the pTrcHisA Rv2873+Rv3875 recombinant fusion plasmid, which was subsequently introduced into the E. coli BL21 strain through transformation. …”
Get full text

The potential contributions of matrix metalloproteinase 8,9 and 13 (MMP-8,9,13) to cerebral vasospasm and the role of doxycycline inhibitors on gene expression after experimental subarachnoid haemorrhage by Yasin Göktürk, Sule Gokturk, Suat E. Çelik

Get full text

HER2-related biomarkers predict clinical outcomes with trastuzumab deruxtecan treatment in patients with HER2-expressing metastatic colorectal cancer: biomarker analyses of DESTINY-CRC01 by Salvatore Siena, Kanwal Raghav, Toshiki Masuishi, Kensei Yamaguchi, Tomohiro Nishina, Elena Elez, Javier Rodriguez, Ian Chau, Maria Di Bartolomeo, Hisato Kawakami, Fumitaka Suto, Makito Koga, Koichiro Inaki, Yusuke Kuwahara, Issey Takehara, Daniel Barrios, Kojiro Kobayashi, Axel Grothey, Takayuki Yoshino

“…Abstract DESTINY-CRC01 (NCT03384940) was a multicentre, open-label, phase 2 study that investigated the safety and efficacy of trastuzumab deruxtecan (T-DXd) in patients with human epidermal growth factor receptor 2 (HER2)-expressing metastatic colorectal cancer (CRC). The present exploratory biomarker analysis aims to investigate relationships between biomarkers and clinical outcomes in patients with HER2-positive (immunohistochemistry [IHC] 3+ or IHC 2+ and in situ hybridization [ISH] positive) Cohort A (N = 53) of DESTINY-CRC01. …”
Get full text

Association of Self-DNA Mediated TLR9-Related Gene, DNA Methyltransferase, and Cytokeratin Protein Expression Alterations in HT29-Cells to DNA Fragment Length and Methylation Status by István Fűri, Ferenc Sipos, Sándor Spisák, Gergő Kiszner, Barnabás Wichmann, Andrea Schöller, Zsolt Tulassay, Györgyi Műzes, Béla Molnár

“…To understand the biologic role of self-DNA bound to Toll-like Receptor 9 (TLR9), we assayed its effect on gene and methyltransferase expressions and cell differentiation in HT29 cells. …”
Get full text

Effects of central administration of the human Tau proteinon the <i>Bdnf, Trkb, p75, Mapt, Bax</i> and <i>Bcl-2</i> genes expression in the mouse brain by A. S. Oreshko, A. Ya. Rodnyy, D. V. Bazovkina, V. S. Naumenko

“…Concurrently, a significant decrease in the expression of the Mapt gene encoding endogenous mouse Tau protein was found in the cerebellum. …”
Get full text

PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression by Caroline N. Rivera, Jason S. Hinkle, Rachel M. Watne, Trent C. Macgowan, Andrew J. Wommack, Roger A. Vaughan

“…PGC-1α regulates the expression of branched-chain amino acid (BCAA) metabolism, and thus, increased circulating BCAA in diabetics may be partially explained by reduced PGC-1α expression. …”
Get full text

Increased Co-Expression of PD-L1 and CTLA-4 Predicts Poor Overall Survival in Patients with Acute Myeloid Leukemia Following Allogeneic Hematopoietic Stem Cell Transplantation by Chen C, Qiu K, Chen J, Wang S, Zhang Y, Wang C, Li Y

“…This study aims to investigate the significance of the alteration of IC co-expression for evaluating the prognosis of AML patients following allogeneic hematopoietic stem cell transplantation (allo-HSCT).Patients and Methods: Quantitative real-time PCR (qRT-PCR) data of bone marrow (BM) samples from 62 de novo AML patients, including 37 patients who received allo-HSCT and 25 patients who received chemotherapy only, were used for prognostic analysis.Results: High expression of PD-1, PD-L1, PD-L2, CTLA-4, and LAG-3 was associated with poor overall survival (OS) in AML patients receiving allo-HSCT, while the expression levels of PD-1, PD-L2, CTLA-4, and LAG-3, other than PD-L1, were not significantly correlated with OS in AML patients receiving chemotherapy. …”
Get full text

Early peripheral blood gene expression (Cyp4A11 and Cyp2E1) in cases of brain ischemia in addict cases admitted to Benha university hospital by Nashwa E. Ahmed, Amina M. Nagah, Omima R. Mohamed, Ghada Mohamed Mahmoud, Sania K. Elwia

Get full text

Expression of Proinflammatory and Regulatory Cytokines via NF-κB and MAPK-Dependent and IFN Regulatory Factor-3-Independent Mechanisms in Human Primary Monocytes Infected by Mycobacterium tuberculosis by Elena Giacomini, Maria Elena Remoli, Marta Scandurra, Valérie Gafa, Manuela Pardini, Lanfranco Fattorini, Eliana M. Coccia

“…We found that Mtb-infected monocytes preferentially express a proinflammatory cytokine profile, including IL-6, TNF-α, and IL-1β. …”
Get full text

Comparative mRNA Expression of eEF1A Isoforms and a PI3K/Akt/mTOR Pathway in a Cellular Model of Parkinson’s Disease by Kawinthra Khwanraj, Suriyat Madlah, Khwanthana Grataitong, Permphan Dharmasaroja

“…In this paper, the expression of all gene members of the PI3K/Akt/mTOR pathway in relation to those of the eEF1A isoforms in a cellular model of PD was investigated at the mRNA level. …”
Get full text

DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression by Rui Hua, Qingjun Chu, Feiyan Guo, Qinjie Chen, Maocai Li, Xuan Zhou, Yongtong Zhu

“…E2F2 expression was upregulated after DNM3OS knockdown in ADR-treated GC-1 spg cells. …”
Get full text

Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation by Chao Wang, Xiao-xia Hou, Hong-liang Rui, Li-jing Li, Jing Zhao, Min Yang, Li-jun Sun, Hong-rui Dong, Hong Cheng, Yi-Pu Chen

“…The experiments in vivo and in vitro both displayed that the mRNA and protein expression of P2X7R, NLRP3, ASC, caspase1 (procaspase-1 mRNA in the gene level and active caspase-1 subunit P10 in the protein level), IL-1β, and IL-18 was significantly upregulated and the mRNA and protein expression of podocyte-associated molecules was significantly changed (downregulation of nephrin, podocin, and WT-1 expression and upregulation of desmin expression) indicating podocyte injury in the kidney tissue of DN rats and in the HG-stressed mouse podocytes, respectively. …”
Get full text

Effect of Interleukin-15 on CD11b, CD54, and CD62L Expression on Natural Killer Cell and Natural Killer T-Like Cells in Systemic Lupus Erythematosus by Syh-Jae Lin, Ji-Yih Chen, Ming-Ling Kuo, Hsiu-Shan Hsiao, Pei-Tzu Lee, Jing-Long Huang

“…NK cells from SLE patients showed higher CD11b and CD62L expression compared to controls. IL-15 enhanced CD11b and CD54 but downregulated CD62L expression on NK cells from SLE patients. …”
Get full text

Cicer arietinum abscisic acid receptor PYL1 and glycine-rich RNA-binding, abscisic acid-inducible protein-like differential expression under induced draught conditions by Slishchuk Heorhii, Volkova Nataliia, Dilafruz Kholmurodova

“…Results showed a significant interaction between genotype and condition for abscisic acid receptor PYL1, where expression increased in the drought-tolerant genotype under stress and decreased in the non-tolerant genotype. …”
Get full text

Neospora caninum infection specifically suppresses the expression of a host lncRNA XR_001919077.1 to facilitate parasite propagation by modulating host cell mitochondrial function and autophagy by Shan-Shan Zhao, De-Liang Tao, Jin-Ming Chen, Ming-Yi Zhang, Xin Yang, Jun-Ke Song, Qun Liu, Guang-Hui Zhao

“…Our previous study found that N. caninum infection induced significantly aberrant expression of lncRNA profiles in caprine endometrial epithelial cells (EECs). …”
Get full text

Impact of 17-alpha ethinyl estradiol (EE2) and diethyl phthalate (DEP) exposure on microRNAs expression and their target genes in differentiated SH-SY5Y cells by Agnese Graziosi, Camilla Corrieri, Giulia Sita, Luca Ghelli, Sabrina Angelini, Roberta d’Emmanuele di Villa Bianca, Emma Mitidieri, Raffaella Sorrentino, Patrizia Hrelia, Fabiana Morroni

“…Using differentiated SH-SY5Y human neuronal cells, we evaluated cell viability, microRNA (miRNA) regulation, and RNA expression following exposure to subtoxic concentrations of EE2 and DEP. …”
Get full text

The expression of apoptosis-regulating proteins Bcl-2 and Bad in liver cells of C57Bl/6 mice under light-induced functional pinealectomy and after correction with melatonin by S. V. Michurina, I. Yu. Ishchenko, S. A. Arkhipov, A. Yu. Letyagin, M. A. Korolev, E. L. Zavjalov

“…The Bad expression area in the liver of LIFP mice increased 4 times against a background of the unchanged Bcl-2 expression area. …”
Get full text

Long non-coding RNA Malat1 modulates CXCR4 expression to regulate the interaction between induced neural stem cells and microglia following closed head injury by Qin Dong, Pengyu Chen, Wenqiao Qiu, Zhijun Yang, Yanteng Li, Yuhui Zhou, Lili Guo, Dan Zou, Ruxiang Xu, Mou Gao

“…Methods We performed bioinformatic analysis to detect the differentially expressed genes in iNSCs after co-cultured with LPS-activated microglia. …”
Get full text

Network and systems biology approaches help investigate gene regulatory interactions between Salmonella disease and host in chickens: Model‐based in silico evidence combined with gene expression assays by Reza Tohidi, Hoda Javaheri Bargourooshi, Arash Javanmard

“…This method normalizes the expression of the target gene to that of actin, an internal control and estimates the change in expression relative to the untreated control. …”
Get full text