The potential for gene-editing to increase muscle growth in pigs: experiences with editing myostatin by A. C. Dilger, X. Chen, L. T. Honegger, B. M. Marron, J. E. Beever

Subjects: “…Gene-editing…”
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Community surveillance and response to maternal and child deaths in low- and middle-income countries: A scoping review. by Tariro J Basera, Kathrin Schmitz, Jessica Price, Merlin Willcox, Edna N Bosire, Ademola Ajuwon, Marjorie Mbule, Agnes Ronan, Fiona Burtt, Esca Scheepers, Jude Igumbor

“…<h4>Background</h4>Civil registration and vital statistics (CRVS) systems do not produce comprehensive data on maternal and child deaths in most low- and middle-income countries (LMICs), with most births and deaths which occur outside the formal health system going unreported. …”
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Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 by Xiaofang Xie, Xiaofang Xie, Yaxuan Zhai, Zhichen Zhu, Feinan Qian, Feinan Qian, Jie Zhu, Qizhao Gao, Hong Du, Hong Du

“…The antimicrobial susceptibility testing (AST) was evaluated through the application of the minimum inhibitory concentration (MIC) methodology by the broth microdilution method. Annotation and analysis of the genome enabled the identification of the plasmid structure and the comparative analysis of the genetic background. …”
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Tackling the unyielding: testing two novel approaches against NDM-producing Enterobacterales and Pseudomonas aeruginosa isolates collected in India by Yamuna Devi Bakthavatchalam, Bijayini Behera, Anand Shah, Purva Mathur, Raja Ray, Bashir Ahmed Fomda, Kamini Walia, Balaji Veeraraghavan

“…Minimum inhibitory concentrations of antibiotics against multicentric NDM-producing Escherichia coli (n = 117), Klebsiella pneumoniae (n = 103), and P. aeruginosa (n = 72) were determined by the reference broth microdilution method. Among E. coli, 111 isolates were NDM-alone, and six were NDM + OXA-48-like producers. …”
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Pattern and Predictors of Maternal Healthcare Services Utilization among Women of Reproductive Age in Lagos, Nigeria by Esther Oluwakemi Oluwole, Alero Ann Roberts, Ifeoma Peace Okafor, Victoria Oluwasola Yesufu

“…The majority (97%) attended ANC more than four times, and 77% gave births in healthcare facilities. Notably, 86% attended postnatal care services primarily for child vaccination. …”
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Nisin A-producing Lactococcus cremoris formulations for pre- and post-milking teat disinfection modulate the bovine milk microbiota by Alessandra Gazzola, Camilla Ceccarani, Bianca Castiglioni, Filippo Biscarini, Stefano Morandi, Tiziana Silvetti, Renata Piccinini, Milena Brasca, Paola Cremonesi

“…Results Clear differences in bacterial community composition were observed among groups, with higher species richness in TR, especially of Staphylococcus, Enterococcus, Lactococcus, and Streptococcus genera. …”
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Burden of early neonatal mortality in Sub-Saharan Africa. A systematic review and meta-analysis. by Natnael Moges, Anteneh Mengist Dessie, Denekew Tenaw Anley, Melkamu Aderajew Zemene, Natnael Atnafu Gebeyehu, Getachew Asmare Adella, Gizachew Ambaw Kassie, Misganaw Asmamaw Mengstie, Mohammed Abdu Seid, Endeshaw Chekol Abebe, Molalegn Mesele Gesese, Yenealem Solomon Kebede, Sefineh Fenta Feleke, Tadesse Asmamaw Dejenie, Natnael Amare Tesfa, Wubet Alebachew Bayih, Ermias Sisay Chanie, Berihun Bantie

“…<h4>Background</h4>Globally, with a neonatal mortality rate of 27/1000 live births, Sub-Saharan Africa has the highest rate in the world and is responsible for 43% of all infant fatalities. …”
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Elementary students' mathematics learning activities and outcomes through the application of the problem solving method by Esty Saraswati Nur Hartiningrum, Nining Yuly Agustin, Rohmatul Umami

“…Pada SDN Payaman, pembelajaran di kelas V masih terpusat pada guru, siswa kurang aktif, dan mengalami kesulitan dalam menyelesaikan soal cerita pada materi Satuan Kecepatan dan Debit. Tujuan dari penelitian ini melihat peningkatan aktivitas dan hasil belajar siswa kelas V SDN Payaman dengan diterapkannya media Roda Satuan dengan metode Problem Solving untuk membuat siswa lebih mudah memahami materi dan penyelesaian soal yang diberikan. …”
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Tetracycline and chloramphenicol exposure induce decreased susceptibility to tigecycline and genetic alterations in AcrAB-TolC efflux pump regulators in Escherichia coli and Klebsi... by Nian Anwar Nasralddin, Mehri Haeili, Sasan Karimzadeh, Fatemeh Alsahlani

“…Susceptibility changes to several antimicrobial agents were tested using disk diffusion and broth dilution methods. The genetic alterations of genes coding for major AcrAB regulators including acrR (repressor of acrAB), ramR (repressor of ramA), soxR (repressor of soxS) in K. pneumoniae and lon (proteolytic degradation of MarA), marR (repressor of marA), acrR and soxR in E. coli were investigated. …”
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Long-term neuropsychologic outcome of pre-emptive mTOR inhibitor treatment in children with tuberous sclerosis complex (TSC) under 4 months of age (PROTECT), a two-arm, randomized,... by Jan H. Driedger, Julian Schröter, PROTECT-Study Group, Steffen Syrbe, Afshin Saffari

“…Abstract Background Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder affecting multiple organ systems, with a prevalence of 1:6,760–1:13,520 live births in Germany. On the molecular level, TSC is caused by heterozygous loss-of-function variants in either of the genes TSC1 or TSC2, encoding the Tuberin-Hamartin complex, which acts as a critical upstream suppressor of the mammalian target of rapamycin (mTOR), a key signaling pathway controlling cellular growth and metabolism. …”
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Antenatal Care - How to ensure quality! by Lubna Ejaz

“…On the other hand, there is only 1.5-fold reciprocal decline in maternal mortality ratio during this time period i.e. from 431/100,000 live births in 1990-91 to 186/100,000 live births in 2017-2018 10, 11. …”
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How can the buffalo carcass be used rationally? by Ricardo Alexandre Silva Pessoa

“…There is a high volume of waste bones, which could be used in food, such as bone broth, for example. The volume of edible trimmings should be better used for making meat in cubes or as bait. …”
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Green synthesis of titanium dioxide photocatalyst using Lactobacillus bulgaricus for processing palm oil mill effluent by L. Agustina, M. Romli, P. Suryadarma, S. Suprihatin

“…This study aims to develop a green synthesis process of nano- titanium dioxide photocatalysts for the advanced treatment of palm oil mill effluent.METHODS: The green synthesis of titanium dioxide nanoparticles used de Man-Rogosa-Sharpe broth media containing Lactobacillus bulgaricus culture and titanium oxyhydroxide metal oxide. …”
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In vitro activity of macrolides against Streptococcus pneumoniae and Streptococcus pyogenes in the Russian Federation: “Status praesens” by Kozlov R.S., Ivanchik N.V., Mikotina A.V., Dekhnich A.V.

“…Susceptibility to antimicrobials was determined by broth microdilution method in accordance with the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST, v.14.0). …”
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Maternal care utilization and provision during the COVID-19 pandemic: Voices from minoritized pregnant and postpartum women and maternal care providers in Deep South. by Ran Zhang, Tiffany Byrd, Shan Qiao, Myriam E Torres, Xiaoming Li, Jihong Liu

“…Participants were recruited from Obstetrics and Gynecology clinics, pediatric clinics, and community health organizations in South Carolina, and all births took place in 2021. Interview transcripts were analyzed thematically.…”
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Multilevel analysis of prevalence and determinants of diarrhea among under-five children in Somalia: insights from the Somalia demographic and health survey 2020 by Hamse Arab Ali, Abdulkadir Mohamed Nuh, Hamse Adam Abdi, Abdisalam Hassan Muse

“…Maternal age also played a significant role, with mothers aged 45–49 presenting higher odds of their children experiencing diarrhea (AOR = 7.650; CI: 1.762–33.184). Institutional births were associated with lower odds of diarrhea (AOR = 0.693; CI: 0.568–0.847), while children of educated fathers exhibited increased odds (AOR = 1.414; CI: 1.090–1.833). …”
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Diversity in Adaptive Evolution of Methicillin-Resistant Staphylococcus aureus Clinical Isolates Under Exposure to Continuous Linezolid Stress in vitro by Han T, Jia T, Wang J

“…Thus, this study aims to explore the adaptive evolution characteristics underlying linezolid resistance in MRSA clinical isolates exposed to continuous induction stress of linezolid in vitro.Methods: The in vitro susceptibility of 1032 MRSA clinical isolates to linezolid was detected using commercial VITEK-2 equipment via broth microdilution. MRSA isolates with different minimum inhibitory concentration (MIC) values for linezolid were randomly selected to perform the assay of adaptive laboratory evolution with sub-inhibitory concentrations of linezolid. …”
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Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study by Zijuan Jian, Yanjun Liu, Zhiqian Wang, Peilin Liu, Jiahui Wang, Qun Yan, Wenen Liu

“…Methods CRKP isolates from twelve hospitals in Central South China were screened for colistin resistance using broth microdilution. The epidemiological characteristics, virulome, resistome, plasmid replicons and two-component systems associated with colistin resistance of colistin-resistant isolates were explored by whole-genome sequencing. …”
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Networks of care for optimizing Primary Health Care Service Delivery in Ethiopia: Enhancing relational linkages and care coordination. by Gizachew Tadele Tiruneh, Nebreed Fesseha, Temesgen Ayehu, Tamar Chitashvili, Mesele Damte Argaw, Biruhtesfa Bekele Shiferaw, Mikias Teferi, Agumasie Semahegn, Biruk Bogale, Yibeltal Kifle, Hillina Tadesse, Chala Tesfaye, Dessalew Emaway

“…Perinatal mortality decreased by 34%, from 31.3 to 20.1 per 1,000 births [t-test: 2.12; p-value: 0.040)].<h4>Conclusion</h4>The NoCs approach in Ethiopia has proven effective in enhancing the relational elements, care coordination, and quality of primary health care services, leading to better maternal and child health outcomes. …”
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Antibacterial and Antifungal Activities of Linear and Cyclic Peptides Containing Arginine, Tryptophan, and Diphenylalanine by David Salehi, Eman H. M. Mohammed, Naiera M. Helmy, Keykavous Parang

“…The minimum inhibitory concentrations (MICs) of all peptides were determined by the micro-broth dilution method against <i>Methicillin-Resistant Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Enterococcus faecium</i>, <i>Enterococcus faecalis</i>, <i>Streptococcus pneumoniae</i>, and <i>Bacillus subtilis</i>. …”
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