Exposure of Mycobacteriodes abscessus clones to mucin affects bacterial phenotype by Amy Leestemaker-Palmer, Tiffany Ong, Luiz E Bermudez

“…To begin understanding how the bacterium adapts to the lung environment, pure colonies of M. abscessus strain 19,977 were grown in 7H9 broth with or without mucin over 30-day intervals for 6 months and analyzed for colony morphology, 7 day-growth curves, the ability to form biofilms after 14 days, and the susceptibility to antibiotics determined using previous published methods. …”
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Genotypic and phenotypic characterization of antimicrobial resistance in Salmonella strains isolated from both No-Antibiotics-Ever (NAE) and conventional broiler complexes by Yagya Adhikari, Dianna V. Bourassa, Sabin Poudel, Matthew A. Bailey, Richard J. Buhr, Kenneth S. Macklin

“…ResFinder was used to identify AMR genes from whole genome sequences while the phenotypic resistance of 14 different antibiotics was determined using broth microdilution method. Odds ratio and 95 % confidence limits were calculated using multivariate logistic regression for different complexes and stages of broiler production (α = 0.05). …”
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Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase by Amrit Koirala, Nabilah Ali Alshibli, Bikram K. Das, Volker S. Brözel

“…As bacterial nitrogenases are extremely sensitive to oxygen, most primary isolation methods rely on the use of semisolid agar or broth to limit oxygen exposure. Without physical separation, only the most competitive strains are obtained. …”
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Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus by Leila Asadpour, Maryam Alsadat Mehrbakhsh Bandari, Roozbeh Sojoudi Masouleh

“…Antibacterial properties of obtained nanoparticles against S. aureus were determined using agar disc diffusion, broth micro dilution methods and also time-kill assay. …”
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Phytochemical Profiling, GC-MS Analysis and Antibacterial Activity of Sida acuta leave Extracts Against Helicobacter pylori by Kamoldeen Ajijolakewu, Ayobami Elias Ajadi, S A Ayoola, Sheriffdeen Issa Bale, Taiwo Ayodeji Sorunke, Saheed Abayomi Onifade, Tomisin Oyawoye

“…The antibacterial activity of the leaf extracts was tested against H. pylori via agar well diffusion and broth dilution using various extract concentrations.  …”
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Establishment and evaluation of a UPLC-MS/MS method for simultaneous determination of bongkrekic acid and dehydroacetic acid in rice noodles by Lingguo Zhao, Ronggui Lv, Ronggui Lv, Jiang Song, Xi Chen, Lei Lei, Weixian Zeng, Miaowen She, Miaowen She, Dabing Li, Xiaxia Yu, Zhanguo Liu, Xiao Wang, Yong Liu

“…The results showed that the established method could also be used to qualitatively screen BA and its isomer isobongkrekic acid (IBA) in GVC enrichment broth, potato glucose agar and rice sample, which facilitates a rapid and accurate judgement of whether the isolated bacterium strain produces BA. …”
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A Prospective, Randomized Comparison of Duodenoscope Reprocessing Surveillance Methods by Travis J. De Wolfe, Nasia Safdar, Megan Meller, John Marx, Patrick R. Pfau, Eric M. Nelsen, Mark E. Benson, Anurag Soni, Mark Reichelderfer, Megan Duster, Deepak V. Gopal

“…The UWHC protocol (n = 119 sampling events) required culturing samples collected from each duodenoscope after swabbing its elevator, immersing its terminal end into broth and flushing its lumen with saline. With the CDC method, 8.53% (n = 11) of the duodenoscopes sampled were positive for bacterial growth with 15 isolates recovered. …”
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The UK Breast Cancer in Pregnancy (UKBCiP) Study. Incidence, diagnosis, management and short-term outcomes of breast cancer first diagnosed during pregnancy in the United Kingdom:... by Marian Knight, Andrew Brand, Claudia Hardy, Philip Banfield, Julie Jones

“…Background The incidence of breast cancer first arising during pregnancy has been estimated in several countries to be 2.4-7.8/100,000 births, but has not been established in the United Kingdom (UK). …”
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Determinants of Facility-Based Childbirth in Indonesia by Ferry Efendi, Ani Rihlatun Ni’mah, Setho Hadisuyatmana, Heri Kuswanto, Linlin Lindayani, Sarni Maniar Berliana

“…One of the challenges is increasing delivery at healthcare facilities to ensure safe and healthy births. In Indonesia, research on factors affecting women’s use of facility-based childbirth services is scarce. …”
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Bubbling insights: unveiling the true sophorolipid biosynthetic pathway by Starmerella bombicola by Sophie L. K. W. Roelants, Stijn Bovijn, Elvira Bytyqi, Nicolas de Fooz, Goedele Luyten, Martijn Castelein, Thibo Van de Craen, Zhoujian Diao, Karolien Maes, Tom Delmulle, Maarten De Mol, Sofie L. De Maeseneire, Bart Devreese, Wim K. Soetaert

“…Bolaform sophorolipids are found in very small amounts in extracellular S. bombicola wild type broths as they are very efficiently converted into lactonic sophorolipids, while acidic sophorolipids build up as they cannot be converted. …”
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The Effect of Maternal Body Mass Index on in Vitro Fertilization-Intracytoplasmic Sperm Injection Treatment: A Prospective Comparative Study by Zeynep Ozturk Inal, Hasan Ali Inal

“…In addition, the numbers of oocytes retrieved (groups 1 vs 3, p=0.011), metaphase II (groups 1 vs 3, p=0.017) and 2 pronucleus (groups 1 vs 3, p=0.010 and groups 2 vs 3, p=0.010), and the rates of clinical pregnancy (40.1% vs 33.2% vs 23.8%, respectively), live births (33.6% vs 23.7% vs 13.9%, respectively), and miscarriages (17.7% vs 28.6% vs 44.7%, respectively) were also different between the groups (p<0.05). …”
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Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala Internati... by Taufik Kakande, Yonah Batunge, Emmanuel Eilu, Ambrose Shabohurira, Justus Abimana, Saheed Adekunle Akinola, Reagan Muhwezi, Abubakar Sunusi Adam, Sarah Kemuma Onkoba, Adamu Almustapha Aliero, Collins Atuheire, Charles Drago Kato, Ibrahim Ntulume

“…Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the ethanolic leaves crude extract were also determined using broth tube dilution and culture, respectively. Out of 100 samples collected, 49 (49%, 95%CI: 0.3930-0.5876) were found positive for microscopy. …”
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Immune-metabolic shifts in acute liver failure caused by HEV infection during pregnancy and their association with obstetric outcomes by Anoushka Saxena, Minal, Prabhjyoti Pahwa, Jaswinder Singh Maras, Hamda Siddiqui, Jayesh Kumar Sevak, Yedla Manikya Mala, Shakun Tyagi, Shiv K. Sarin, Nirupama Trehanpati

“…ALF-E showed increased naive T/B cells, decreased CD4, CD8 Tcm, Tem, and plasmablasts, compared to AVH-E contributing to higher failed inductions, preterm births, maternal complications like eclampsia, disseminated intravascular coagulation, preterm premature rupture of membranes, small-for-gestational-age infants, higher rates of intrauterine death, abortion, and mortality. …”
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Prediction model study focusing on eHealth in the management of urinary incontinence: the Personalised Advantage Index as a decision-making aid by Huibert Burger, Henk van der Worp, Marco H Blanker, Marjolein Y Berger, Anne Martina Maria Loohuis, Nienke Wessels, Janny Dekker, Alec GGA Malmberg

“…Of the 350 screened women, 262 were eligible and randomised to app-based treatment or care as usual; 195 (74%) attended follow-up.Predictors Literature review and expert opinion identified 13 candidate predictors, categorised into two groups: Prognostic factors (independent of treatment type), such as UI severity, postmenopausal state, vaginal births, general physical health status, pelvic floor muscle function and body mass index; and modifiers (dependent on treatment type), such as age, UI type and duration, impact on quality of life, previous physical therapy, recruitment method and educational level.Main outcome measure Primary outcome was symptom severity after a 4-month follow-up period, measured by the International Consultation on Incontinence Questionnaire the Urinary Incontinence Short Form. …”
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Exploring the antibacterial and anti-biofilm activity of two Iranian medical-grade kinds of honey on multidrug-resistant Pseudomonas aeruginosa by Mustafa Riyadh Alshaybawee, Shahrzad Asgari, Paria Ghadersoltani, Ahmadreza Mehrabian, Parastoo Saniee

“…Abstract Introduction Pseudomonas aeruginosa is a prominent multidrug-resistant and biofilm-forming bacteria. Mono-floral honey, enriched with a variety of biological compounds, can be categorized as medical-grade honey due to its notable pharmacological benefits. …”
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Non-utilisation of postpartum services in northwestern Nigeria: analyses of trend and social determinants from 2003 to 2018 by Dyah Anantalia Widyastari, Abubakar Abbani Yakubu, Yothin Sawangdee, Pojjana Hunchangsith

“…The odds of not using postpartum services were higher for women with no education (adjusted OR (AOR): 1.27; CI: 1.03 to 1.58), those with no knowledge of contraceptives (AOR: 1.72; CI: 1.35 to 2.19), those who never used contraceptives (AOR: 1.71; CI: 1.39 to 2.09), those with parity of four or more births (AOR: 1.58; CI: 1.34 to 1.86), those in polygynous marriage (AOR: 1.16; CI: 1.03 to 1.30) and those from the poorest (AOR: 2.34; CI: 1.67 to 3.28) and poorer (AOR: 2.05; CI: 1.50 to 2.78) households. …”
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A randomised trial comparing preoperative administration of single-dose kefazolin to kefazolin plus metronidazole as prophylactic antibiotics at caesarean section by R Lamfel, L Snyman, L Seopela, G Jahn, P Becker

“…It has been estimated that globally, 29.7 million births occur by caesarean section annually. The risk of postpartum infection is estimated to be five to ten times higher compared with normal vaginal delivery. …”
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Genomic Characterization of Carbapenemase-Producing Enterobacteriaceae from Clinical and Epidemiological Human Samples by Alexander Tristancho-Baró, Laura Eva Franco-Fobe, Monica Pilar Ariza, Ana Milagro, Ana Isabel López-Calleja, Blanca Fortuño, Concepción López, Miriam Latorre-Millán, Laura Clusa, Rosa Martínez, Carmen Torres, Antonio Rezusta

“…Antibiotic susceptibility testing was performed by broth microdilution. Whole-genome sequencing (WGS) was performed using Illumina short reads. …”
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Incidence of methicillin, inducible clindamycin, and vancomycin resistance in Staphylococcus aureus clinical isolates in Gombe, Northeastern Nigeria by Haruna Adamu, Celestina T. Otegwu, Fatima B. Abdullahi, Babajide A. Tytler, Busayo O. Olayinka

“…Antibiotic susceptibility testing was conducted via modify Kirby Bauer disc diffusion and the macro broth dilution method. The species-specific 16S rRNA gene was investigated in the presumptive identified S. aureus isolates whereas the mecA and vanA genes were investigated in cefoxitin and vancomycin resistant S. aureus isolates via PCR. …”
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A dextrorotatory residues-incorporated bioactive dodecapeptide against enterohemorrhagic Escherichia coli by Ping Zeng, Xuemei Yang, Kwok-Yin Wong, Sheng Chen, Kin-Fai Chan, Sharon Shui Yee Leung, Lanhua Yi

“…A double dilution method was applied to investigate its efficacy in normal broth medium, serum, and highly saline conditions. …”
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