A comprehensive overview of focused ion beam-scanning electron microscopy (FIB-SEM) applications for the evaluation of outer retina by Sanjay Ch, Rayne R. Lim, Shermaine W. Y. Low, Deana G. Grant, Sam Patterson, Aparna Ramasubramanian, Ashish K. Gadicherla, Shyam S. Chaurasia, Shyam S. Chaurasia

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Optimized protocol to preserve RNA integrity for laser capture microdissection of bovine mammary epithelial cells by Ratan Kumar Choudhary, Thomas B. McFadden, Erin M. Shangraw, Feng-Qi Zhao

“…Laser capture microdissection (LCM) is a popular technique for isolating specific cell types from tissues for medical research. However, the LCM procedure for isolating bovine mammary epithelial cells (MEC) for high-throughput transcriptome profiling has been lacking. …”
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Investigating the effects of outer membrane vesicles derived from Campylobacter jejuni on the survival of HT-29 epithelial cells in vitro by Elham Khodamoradi, Dariush Minai-Tehrani, Abbas Yadegar

“…C. jejuni OMVs were isolated using ultracentrifugation and were analyzed by scanning electron microscopy (SEM) and dynamic light scattering (DLS). HT-29 human colon cancer epithelial cells were treated with OMVs for 24, 48, and 72 hours. …”
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Cilomilast Modulates Rhinovirus-Induced Airway Epithelial ICAM-1 Expression and IL-6, CXCL8 and CCL5 Production by Jie Zhu, Michael R. Edwards, Simon D. Message, Luminita A. Stanciu, Sebastian L. Johnston, Peter K. Jeffery

“…<b>Methods:</b> BEAS-2B bronchial epithelial cells were incubated with 0.5–2 MOI (multiplicity of infection–infectious units/cell) of rhinovirus 16 (RV16). …”
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Stem Cells Within Three-Dimensional-Printed Scaffolds Facilitate Airway Mucosa and Bone Regeneration and Reconstruction of Maxillary Defects in Rabbits by Mi Hyun Lim, Jung Ho Jeon, Sun Hwa Park, Byeong Gon Yun, Seok-Won Kim, Dong-Woo Cho, Jeong Hak Lee, Do Hyun Kim, Sung Won Kim

“…<i>Results</i>: In vitro, hNTSCs differentiated into both mucosal epithelial and osteogenic cells. hNTSC differentiation into respiratory epithelial cells was confirmed by Alcian Blue staining, cilia in SEM, and increased expression levels of FOXJ1 and E-cadherin through quantitative RT-PCR. hNTSC differentiation into bone was confirmed by Alizarin Red staining, increased mRNA expression levels of BMP2 (6.1-fold) and RUNX2 (2.3-fold) in the hNTSC group compared to the control. …”
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Protocols to co-culture human primary lung cells in the simple-flow device by Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottier

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Histogenesis of the Uterine Horn in the Domestic Cat (<i>Felis silvestris catus</i>): LM, TEM, and SEM Study by Ewelina Prozorowska-Basińska, Marlena Ratajczak, Hanna Jackowiak

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Tips and tricks for gut microbiota investigation using scanning electron microscopy (SEM): going from sample preparation to imaging and landscape analysis by Meriem Boukili, Omar Zmerli, Florence Fenollar, Sara Bellali, Jacques Bou Khalil

“…CDI group revealed a reduction in microbial diversity, compared to the controls. Epithelial and red blood cells were more prevalent in CDI group. …”
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Bezlotoxumab prevents extraintestinal organ damage induced by Clostridioides difficile infection by Steven J. Mileto, Melanie L. Hutton, Sarah L. Walton, Antariksh Das, Lisa J. Ioannidis, Don Ketagoda, Kylie M. Quinn, Kate M. Denton, Diana S. Hansen, Dena Lyras

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Comprehensive Advanced Physicochemical Characterization and In Vitro Human Cell Culture Assessment of BMS-202: A Novel Inhibitor of Programmed Cell Death Ligand by Hasham Shafi, Andrea J. Lora, Haley M. Donow, Sally E. Dickinson, Georg T. Wondrak, H.-H. Sherry Chow, Clara Curiel-Lewandrowski, Heidi M. Mansour

“…In vitro safety assessments involved testing with skin epithelial cell lines, such as HaCaT and NHEK, and Transepithelial Electrical Resistance (TEER) to evaluate barrier integrity. …”
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Disruption of Vps4 and JNK function in Drosophila causes tumour growth. by Lina M Rodahl, Kaisa Haglund, Catherine Sem-Jacobsen, Franz Wendler, Jean-Paul Vincent, Karine Lindmo, Tor Erik Rusten, Harald Stenmark

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The influence of femtosecond laser intrastromal lenticules on the characteristics and maturity in tissue-engineered stem cell-derived retinal pigment epithelium sheets by Gu Jianing, Su Zhanyu, Wang Yini, Chen Yuexi, Cui Zekai, Li Shengguo, Ding Chengcheng, Sheng Wang, Li Kangjun, Tang Shibo, Chen Jiansu

“…Conclusions We determine that engineered RPE sheets using dfLEN scaffolds enhance RPE characteristics and functions, and suggest that dfLEN scaffolds promote cilium process maturation and polarization of iPSC-derived epithelial cells. Such a strategy for constructing iRPE sheets holds significant potential for advancing RPE cell therapy, disease models, and drug screening platforms.…”
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Therapeutic potential of placenta-derived stem cells cultivated on noggin-loaded nanochitosan/polypyrrole-alginate conductive scaffold to restore spinal cord injury by Asma Manzari-Tavakoli, Amirhesam Babajani, Nasim Vousooghi, Ali Moghimi, Roghayeh Tarasi, Fahimeh Safaeinejad, Samira Norouzi, Soheyl Bahrami, Hassan Niknejad

“…This study aims to investigate the potential of a nanochitosan/polypyrrole-alginate conductive scaffold, loaded with the Noggin growth factor, an inhibitor of BMP-4 signaling, and human amniotic epithelial cells (hAECs), in promoting the regeneration of SCI in animal models. …”
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Physicochemical and Toxicological Properties of Particles Emitted from Scalmalloy During the LPBF Process by Nikoletta Sargioti, Leonidas Karavias, Leonidas Gargalis, Anna Karatza, Elias P. Koumoulos, Evangelia K. Karaxi

“…In vitro cytotoxicity evaluations using A549 lung epithelial cells showed significant reductions in cell viability (~60 to 69%) and increased oxidative stress (<i>p</i> < 0.05) upon exposure to virgin sieved (<20 µm) and exhaust powder samples. …”
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A novel PNIPAM-Modified polyurethane/carboxymethyl cellulose photo-thermoresponsive hydrogel loaded with gemcitabine to suppress esophageal cancer cells via VEGF-mediated angiogeni... by Shirong Wu, Huachuan Zhang, Yu Zhang, Ya Zhao, Mengqi Xiang, Liqiong Hao, Jing Chen

“…Biocompatibility assessment using the normal human esophageal epithelial cell line Het-1 A demonstrated increased cell viability (85%) without showing any morphological changes when treated with fabricated hydrogel formulations, confirming the non-toxic nature of the carrier system. …”
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Comparing the Effects of Various Implant Abutment Materials on Gingival Fibroblast Attachment In Vitro by Mallikarjun H. Doddamani, Manish Varma, Sumit Bhatt, Veena Raghunath, A. J. Ashmi, Nirupama Vemali

“…Gingival fibroblasts were cultured on these specimens in a controlled environment, and cell attachment was assessed using a fluorescence microscopy assay and scanning electron microscopy (SEM). …”
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Hypoxic BMSC-derived exosomal miR-210-3p promotes progression of triple-negative breast cancer cells via NFIX-Wnt/β-catenin signaling axis by Meng Wang, Yi Zheng, Qian Hao, Guochao Mao, Zhijun Dai, Zhen Zhai, Shuai Lin, Baobao Liang, Huafeng Kang, Xiaobin Ma

“…Exosomes play a vital role in cell-to-cell communication within the TME. Hypoxic TME have a great impact on the release, uptake and biofunctions of exosomes. …”
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Optimization of In-Situ Exosome Enrichment Methodology On-a-Chip to Mimic Tumor Microenvironment Induces Cancer Stemness in Glioblastoma Tumor Model by Saleheh Saffar, Ali Ghiaseddin, Shiva Irani, Amir Ali Hamidieh

“…Characterization techniques (SEM, AFM, DLS, RT-qPCR, and ELISA) confirmed significant alterations in exosome profiles, cancer stemness, and epithelial-mesenchymal transition (EMT)-related markers. …”
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Three-Dimensional Printing of Hydrogel as Skin Substitute and Comparative Evaluation of Melanin Production by Mohammad Zafaryab, Komal Vig

“…SEM analysis was performed for the cells present in gel and the topology of the cells. …”
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Three-dimensional ultrastructural analysis of human skin with the arrector pili muscle interacting with the hair follicle epithelium by Tomonobu Ezure, Kyoichi Matsuzaki, Hidetoshi Urakubo, Nobuhiko Ohno

“…Additionally, the dense nerve network running through and along arrector pili muscle fibers rarely penetrates the connective tissues between smooth muscle fibers and epithelial cells. Furthermore, in the observation area, no individual smooth muscle fibers formed adhesion structures with the epithelial cells of the hair follicle, ending in the dermal extracellular matrix near the epithelial cells. …”
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