New insights into the DNA extraction and PCR amplification of minute ascomycetes in the genus Laboulbenia (Pezizomycotina, Laboulbeniales) by Warre Van Caenegem, Danny Haelewaters

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Epigenetic «probes» for lung cancer monitoring: LINE-1 methylation pattern in blood- circulating DNA by A. A. Ponomaryova, Е. Y. Rykova, N. V. Cherdyntseva, A. A. Bondar, A. Y. Dobrodeev, A. A. Zavyalov, S. A. Tuzikov, L. O. Bryzgalov, T. I. Merkulova, V. V. Vlassov, P. P. Laktionov

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Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects by M. O. Lebedev, L. A. Yarinich, A. V. Ivankin, A. V. Pindyurin

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Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana by Sayo Kashiwagi, Isao Kuraoka, Yoshie Fujiwara, Kenichi Hitomi, Quen J. Cheng, Jill O. Fuss, David S. Shin, Chikahide Masutani, John A. Tainer, Fumio Hanaoka, Shigenori Iwai

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DNA Ploidy and S-Phase Fraction in Carcinoma of the Gallbladder Related to Histopathology, Number of Gallstones and Survival 1 by Ulf Gustafsson, Curt Einarsson, Lennart C. Eriksson, Virgil Gadaleanu, Staffan Sahlin, Bernhard Tribukait

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Genomic 8-oxoguanine modulates gene transcription independent of its repair by DNA glycosylases OGG1 and MUTYH by Tobias Obermann, Teri Sakshaug, Vishnu Vignesh Kanagaraj, Andreas Abentung, Mirta Mittelstedt Leal de Sousa, Lars Hagen, Antonio Sarno, Magnar Bjørås, Katja Scheffler

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Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL) by Marcus Wäneskog, Emma Elise Hoch-Schneider, Shilpa Garg, Christian Kronborg Cantalapiedra, Elena Schäfer, Michael Krogh Jensen, Emil Damgaard Jensen

“…However, transformation efficiencies have a direct correlation with the probability of introducing multiple DNA molecules into each cell, although reliable library screenings require cells that contain a single unique genotype. …”
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Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer by Christian Arstad, Kristin Taskén, Paulo Refinetti, Ulrika Axcrona, Karl-Erik Giercksky, Per Olaf Ekstrøm

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Changes in DNA methylation profile in liver tissue during progression of HCV-induced fibrosis to hepatocellular carcinoma by I. A. Goncharova, A. A. Zarubin, N. P. Babushkina, I. A. Koroleva, M. S. Nazarenko

“…Among the genes hypermethylated in tumor tissue in the setting of liver cirrhosis, the most represented biological pathways are developmental processes, cell-cell signaling, transcription regulation, Wnt-protein binding. …”
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Comprehensive Screening of Gene Function and Networks by DNA Microarray Analysis in Japanese Patients with Idiopathic Portal Hypertension by Kohei Kotani, Joji Kawabe, Hiroyasu Morikawa, Tomohiko Akahoshi, Makoto Hashizume, Susumu Shiomi

“…Analysis using network-based pathways revealed decreased expression of adenosine deaminase, ectonucleoside triphosphate diphosphohydrolase 4, ATP-binding cassette, subfamily C, member 1, transforming growth factor-β, and prostaglandin E receptor 2; increased expression of cytochrome P450, family 4, subfamily F, polypeptide 3, and glutathione peroxidase 3; and abnormalities in the immune system, nucleic acid metabolism, arachidonic acid/leukotriene pathways, and biological processes. These results suggested that IPH involved compromised function of immunocompetent cells and that such dysfunction may be associated with abnormalities in nucleic acid metabolism and arachidonic acid/leukotriene-related synthesis/metabolism.…”
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Investigating DNA Methylation of Solute Carrier Genes in Colorectal Cancer: A Comprehensive Analysis Using Microarray and Bioinformatics Tools by Nur Alia Natasya Zabidi, Rashidah Baharudin, Nurul Qistina Rus Bakarurraini, Nur Alyaa Afifah Md Shahri, Francis Tieng Yew Fu, Imilia Ismail, Muhammad Redha Abdullah Zawawi, Jodi Woan-Fei Law, Learn-Han Lee, Nurul-Syakima Ab Mutalib

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Biological Characteristics and Whole-Genome Analysis of a Porcine <i>E. coli</i> Phage by Shenghui Wan, Nana Li, Sajid Habib, Pei Zheng, Yanfang Li, Yan Liang, Yonggang Qu

“…The nucleic acid type of phage Psq-1 was dsDNA, with a total genome length of 44,183 bp and a GC content of 52.16%. …”
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The dynamics and longevity of circulating CD4+ memory T cells depend on cell age and not the chronological age of the host. by M Elise Bullock, Thea Hogan, Cayman Williams, Sinead Morris, Maria Nowicka, Minahil Sharjeel, Christiaan van Dorp, Andrew J Yates, Benedict Seddon

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Mms22-Rtt107 axis attenuates the DNA damage checkpoint and the stability of the Rad9 checkpoint mediator by Bingbing Wan, Danying Guan, Shibai Li, Tzippora Chwat-Edelstein, Xiaolan Zhao

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Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression. by Ankita Chavan, Lena Skrutl, Federico Uliana, Melanie Pfister, Franziska Brändle, Laszlo Tirian, Delora Baptista, Dominik Handler, David Burke, Anna Sintsova, Pedro Beltrao, Julius Brennecke, Madhav Jagannathan

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Editorial: Activation of the cGAS-STING pathway by extra-nuclear DNA and its pharmacognostic modulation in human disease by Preston M. McCourt, Charles A. Day, Souren Paul, Souren Paul

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Insulin signaling regulates R2 retrotransposon expression to orchestrate transgenerational rDNA copy number maintenance by Jonathan O. Nelson, Alyssa Slicko, Amelie A. Raz, Yukiko M. Yamashita

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DNA Ligase IV Prevents Replication Fork Stalling and Promotes Cellular Proliferation in Triple Negative Breast Cancer by Rashmi R. Joshi, Sk Imran Ali, Amanda K. Ashley

“…We previously established that DNA-PKcs, a NHEJ protein, promotes genomic stability and cell viability following cellular exposure to replication stress; we wanted to discern whether another NHEJ protein, DNA ligase IV (Lig4), shares this phenotype. …”
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Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends by Alison B. Hickman, Laurie Lannes, Christopher M. Furman, Christina Hong, Lidiya Franklin, Rodolfo Ghirlando, Arpita Ghosh, Wentian Luo, Parthena Konstantinidou, Hernán A. Lorenzi, Anne Grove, Astrid D. Haase, Matthew H. Wilson, Fred Dyda

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Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification by Dar Gulam Jeelani, Nazir Ruqeya, Wani Shakil A., Farooq Saleem, Aziz Tariq, Albekairi Thamer H.

“…Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the target gene and minimize deoxyribonucleic acid (DNA) degradation. …”
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