18F-Fluorothymidine-Pet Imaging of Glioblastoma Multiforme: Effects of Radiation Therapy on Radiotracer Uptake and Molecular Biomarker Patterns by Sanjay Chandrasekaran, Andrew Hollander, Xiangsheng Xu, Joseph L. Benci, James J. Davis, Jay F. Dorsey, Gary Kao

“…Conventional 18F-FDG-PET imaging is of limited usefulness for imaging Glioblastoma Multiforme (GBM) due to high levels of glucose uptake by normal brain and the resultant signal-to-noise intensity. 18F-Fluorothymidine (FLT) in contrast has shown promise for imaging GBM, as thymidine is taken up preferentially by proliferating cells. These studies were undertaken to investigate the effectiveness of 18F-FLT-PET in a GBM mouse model, especially after radiation therapy (RT), and its correlation with useful biomarkers, including proliferation and DNA damage. …”
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Resiquimod induces a mixed Th1 and Th2 response via STAT1 and STAT3 signalling in chickens by Deepthi Kappala, Saravanan Ramakrishnan, Patel Nikunjkumar Prakashbhai, Abinaya Kaliappan, Prasad Thomas, Jörg Linde, Mithilesh Singh, Sohini Dey, Madhan Mohan Chellappa

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Lack of activity of HIV-1 integrase strand-transfer inhibitors on recombinase activating gene (RAG) activity at clinically relevant concentrations by Sally Demirdjian, Vincent N. Duong, Jennifer N. Byrum, Arabinda Nayak, Cooper B. McKinney, Jason K. Perry, Christian Callebaut, Karla K. Rodgers, Brie Falkard, Joy Y. Feng

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Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province by Monica Septiani, Vetnizah Juniantito, Bambang Pontjo Priosoeryanto

“…African Swine Fever (ASF) is a disease affecting pigs, caused by a double-stranded DNA virus that is not transmissible to humans or other animals. …”
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Alphaherpesvirus in Pets and Livestock by Shu-Hui Duan, Ze-Min Li, Xue-Jie Yu, Dan Li

“…Herpesviruses are a group of DNA viruses capable of infecting multiple mammalian species, including humans. …”
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Characteristics of the monkeypox virus isolate obtained from the first patient in Russia and its sensitivity to 7-[N-(4-trifluoromethylbenzoyl)-hydrazinocarbonyl]-tricyclo-[3.2.2.0... by Alena S. Ovchinnikova, Dmitrii A. Odnoshevsky, Alexey S. Kabanov, Sergey A. Bodnev, Oleg V. Pyankov, Oksana P. Os'kina, Maria V. Sivay, Andrey V. Bespalov, Tatyana V. Tregubchak, Larisa N. Shishkina, Oleg S. Taranov, Vladimir V. Zolin, Artemiy A. Sergeev, Alexander P. Agafonov

“…The disease was confirmed by PCR followed by determination of the nucleotide sequence of viral DNA by sequencing. Isolation of the new MPXV strain from clinical samples was carried out in Vero E6 cells. …”
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Production and bioactivity test of recombinant protein common carp growth hormone by Deny Sapto Chondro Utomo, . Alimuddin, Agus Oman Sudrajat, Irvan Faizal

“… This study aimed to produce recombinant growth hormone (rGH) protein of common carp (Cyprinus carpio) and evaluate its bioactivity. DNA fragment encoding mature GH protein of common carp (mCcGH) was amplified by polymerase chain reaction (PCR) method and PCR products were then ligated into pCold I to generate pCold I-mCcGH protein expression vector. …”
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A new strategy for the treatment of advanced ovarian cancer: utilizing nanotechnology to regulate the tumor microenvironment by Zixuan Xiong, Yichun Huang, Shulong Cao, Xuqun Huang, Haiyuan Zhang

“…In the immune TME, the immune effector cells such as dendritic cells (DCs), M1-like tumor-associated macrophages (M1-TAMs), and T cells are inhibited. …”
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Erwinia phage Asesino is a nucleus-forming phage that lacks PhuZ by Amy Prichard, Annika Sy, Justin Meyer, Elizabeth Villa, Joe Pogliano

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THE EFFECT OF QUERCETIN ON MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN RAT LIVER UNDER 270TH DAY CENTRAL DEPRIVATION OF LUTEINIZING HORMONE SYNTHESIS by M.V. Rud, V.I. Shepitko, Ye.V. Stetsuk, O.Ye. Akimov

“…Peroxynitrite and superoxide anion radical are powerful oxidants that can damage biological polymers (DNA, proteins and biological membranes) and lead to the development of oxidative-nitrosative stress.…”
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Molecular techniques for cancer diagnostics by Vimal Kishor Singh, Ramesh Chandra

“… Cancer cells differ from healthy tissues in their biological makeup. …”
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Ticam2 ablation facilitates monocyte exhaustion recovery after sepsis by Blake A. Caldwell, Susanti Ie, Amy Lucas, Liwu Li

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Fast and sensitive multivalent spatial pattern-recognition for circular RNA detection by Zhixin Zhou, Bing Han, Yu Wang, Nina Lin, Zhongqiu Zhou, Yuan Zhang, Ying Bai, Ling Shen, Yanfei Shen, Yuanjian Zhang, Honghong Yao

“…Here, we engineer an intelligent tetrahedral DNA framework (TDF) possessing precise spatial pattern-recognition properties with exceptional sensing speed and sensitivity for circRNAs. …”
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Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential by Sivasamy Gomathi, Ponnusamy Sasikumar, Kolandaswamy Anbazhagan, Sundaresan Sasikumar, Murugan Kavitha, M. S. Selvi, Govindan Sadasivam Selvam

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Experimental and computational studies of Schiff bases derived from 4-aminoantipyrine as potential antibacterial and anticancer agents by Johana Zúñiga-Miranda, Rebeca González-Pastor, Saskya E. Carrera-Pacheco, Cristina Rodríguez-Pólit, Carlos Barba-Ostria, Antonio Machado, Linda P. Guamán, Christian D. Alcivar-León, Jorge Heredia-Moya

“…Abstract Schiff bases are organic compounds recognized for their biological activities, including antiviral, antibacterial, antifungal, and anticancer properties, making them promising candidates in medicinal chemistry. …”
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Glucocorticoid receptor gene (NR3C1) methylation, childhood maltreatment, multilevel reward responsiveness and depressive and anxiety symptoms: A neuroimaging epigenetic study by Yajing Xu, Shan Yang, Cong Cao

“…Cytosine methylation in the promoter exon 1F of NR3C1 (NR3C1-1F) was sequenced via buccal cells. Childhood maltreatment, self-reported RR and depressive and anxiety symptoms were assessed via questionnaires. …”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Identification of core biomarkers for tuberculosis progression through bioinformatics analysis and in vitro research by Zhanpeng Chen, Qiong Wang, Quan Ma, Jinyun Chen, Xingxing Kong, Yuqin Zeng, Lanlan Liu, Shuihua Lu, Xiaomin Wang

“…Gene Ontology (GO) enrichment analysis revealed that the biological functions of these genes mainly involve DNA translation, RNA processing, and transcriptional regulation. …”
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Therapeutic potential of the neutralizing monoclonal antibody 45G3 against encephalomyocarditis virus by Yanfang Zhang, Zhiying Wang, Yaohui Fang, Qiong Zhu, Jie Fu, Sijing Hu, Jiayin Jin, Min Zhou, Xijia Liu, Danna Zhang, Shouwei Huang, Yali Deng, Lingling Xie, Shu Shen, Jing Ye, Fei Deng, Shengbo Cao

“…In this study, we evaluated the neutralizing effects of a monoclonal antibody derived from the spleens of mice immunized with EMCV virus-like particles (VLPs), both in vitro and in vivo. Using recombinant DNA technology, we engineered a baculovirus system to express EMCVs P12A and 3C, facilitating the production of VLPs in Sf9 cells. …”
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