The impact of PD-L1 polymorphisms on the efficacy of immune checkpoint inhibitors depends on the tumor proportion score: a retrospective study by Keiichiro Suminaga, Takashi Nomizo, Hironori Yoshida, Hiroaki Ozasa

“…Method Patients treated with immune checkpoint inhibitor monotherapy for non-small cell lung cancer at Kyoto University Hospital until January 2023, with TPS data and biological specimens available for SNP measurement, were eligible for this study. …”
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Decoding the general role of tRNA queuosine modification in eukaryotes by Jorge Díaz-Rullo, Luis González-Moreno, Araceli Del Arco, José Eduardo González-Pastor

“…Here, through bioinformatics analysis of representative eukaryotic genomes we have predicted: i) the genes enriched in NAU codons, whose translation would be affected by tRNA Q-modification (Q-genes); and ii) the specific biological processes of each organism enriched in Q-genes, which generally in eukaryotes would be related to ubiquitination, phosphatidylinositol metabolism, splicing, DNA repair or cell cycle. …”
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The Inhibitory Effects of Alpha 1 Antitrypsin on Endosomal TLR Signaling Pathways by Ahmed S. Elshikha, Georges Abboud, Rigena Avdiaj, Laurence Morel, Sihong Song

“…Specifically, hAAT completely blocked the R848 induction of germinal center T cells (GC T), B cells (GC B), and plasma cells (GC PCs), as well as T follicular T helper cells (T_FH), which are all critical in lupus development. …”
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A comprehensive analysis of molecular characteristics of hot and cold tumor of gastric cancer by Chenxi Lv, Tianwei Chen, Jiangtao Li, Yuqiang Shan, Hong Zhou

“…High-risk groups demonstrated strong associations with immune checkpoint regulation, antigen presentation, and inhibitory pathways. Notably, single-cell analysis revealed that HTTR genes were highly active in CD8 + T cells, with the CXCL12-CXCR4 axis playing a critical role in mediating interactions between CD8 + T cells and endothelial cells. …”
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Comprehensive genomic and transcriptomic profiling of pulmonary nodules in synchronous multiple primary lung cancer by Xingsheng Liu, Kun Qian, Lei Su, Xiaoru Tian, Xin Zhao, Tengteng Wang, Li Han, Zhenzhen Li, Peilong Zhang, Ruotian Wang, Baodong Liu, Yuanbo Li, Xiaogang Tan, Yi Zhang

“…The major nodules exhibited heightened activity in tumor cell proliferation pathways and demonstrated malignancy-related biological characteristics, which correlated with pathological assessment results.…”
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Comprehensive epidemiological evaluation of ruminant brucellosis and associated risk factors in some Egyptian Governorates by Nesreen Allam Tantawy Allam, Mahinour Ezzeldin Abdelsalam, Hend I. Elsharkawy, Mai Mohamed Kandil, Amany Mohamed Mohamed Mohamed, Fatma Ali, Mohamed A. Gebely, Safaa Y. Nour, Doaa Sedky, Mona Ebrahim Hussien Abd El-Gawad, Hoda M. Zaki, Nazek Al-Gallas, Amal M. Aboelmaaty, Mona Mohamed Sobhy, Nagwa Sayed Ata, Marwa Salah Abdel-Hamid, Ghada A. Badawy

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Association between non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio and telomere length: the NHANES 1999–2002 by Mingjie Liu, Chendong Wang, Bai Wei

“…TL was measured as the telomere-to-standard reference DNA ratio. Multivariate linear regression and smooth curve fitting were employed to assess the association between NHHR and TL.ResultsThe study population (mean age 45.1 ± 0.4 years, 48.9% male) was stratified into NHHR tertiles. …”
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Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya by Sani Iliya, Jonathan Mwangi, Ronald Maathai, Mary Muriuki, Christopher Wainaina

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Novel Antibacterial 4-Piperazinylquinoline Hybrid Derivatives Against <i>Staphylococcus aureus</i>: Design, Synthesis, and In Vitro and In Silico Insights by Gabriele La Monica, Annamaria Gallo, Alessia Bono, Federica Alamia, Antonino Lauria, Rosa Alduina, Annamaria Martorana

“…Further in silico docking and molecular dynamics studies highlighted strong interactions of <b>5k</b> with bacterial enzymes, such as tyrosyl-tRNA synthetase, pyruvate kinase, and DNA gyrase B, suggesting potential modes of action. …”
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CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis by Jianqiang Wu, Lixia Chen, Peijun Wen

“…Functionally, CRIP1 inhibits the proliferation, migration, and invasion of melanoma cells in vitro. Mechanistic studies revealed that CRIP1 inhibited mitochondrial biogenesis in melanoma cells, which included suppression of relative mitochondrial content, mitochondrial DNA copy number, ATP production, respiratory capacity, and expression levels of oxidative phosphorylation-related proteins. …”
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Natural repeated backcrosses lead to triploidy and tetraploidy in parthenogenetic butterfly lizards (Leiolepis: Agamidae) by Eduard Galoyan, Roman Nazarov, Marie Altmanová, Sergey Matveevsky, Ivan Kropachev, Dmitrij Dedukh, Eugene Iryshkov, Mark Pankin, Natalia Sopilko, Oleg Nikolaev, Nikolai Orlov, Marine Arakelyan, Jiří Klíma, Evgeniya Solovyeva, Tao Nguyen, Lukáš Kratochvíl

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Integrated and streamlined microfluidic device for molecular diagnosis of pathogen through direct PCR amplification by Dohwan Lee, Tae Seok Seo

“…Direct PCR, which enables direct DNA amplification from biological samples without the need for DNA extraction and purification, streamlines the device architecture and transforms it into a more user-friendly form. …”
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Microsatellite instability and somatic gene variant profile in solid organ tumors by Ibrahim Halil Erdogdu, Seda Orenay-Boyacioglu, Olcay Boyacioglu, Nesibe Kahraman-Cetin, Habibe Guler, Merve Turan, Ibrahim Meteoglu

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Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury by Vladislav N. Karkischenko, Veronika I. Skvortsova, Melik T. Gasanov, Yuriy V. Fokin, Maxim S. Nesterov, Nataliya V. Petrova, Oxana V. Alimkina, Igor A. Pomytkin

“…HMGB1 is a ubiquitous nuclear DNA-binding protein whose biological function depends on posttranslational modifications, its redox state, and its cellular localization. …”
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Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage by Ying Zheng, Xi Luo, Zailong Qin, Zhiguang Zhou

“…A series of inflammatory cytokines are produced and released to eliminate pathogens. CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. …”
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The <i>Meq</i> Genes of Nigerian Marek’s Disease Virus (MDV) Field Isolates Contain Mutations Common to Both European and US High Virulence Strains by Joseph N. Patria, Luka Jwander, Ifeoma Mbachu, Levi Parcells, Brian Ladman, Jakob Trimpert, Benedikt B. Kaufer, Phaedra Tavlarides-Hontz, Mark S. Parcells

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Comprehensive computational analysis of differentially expressed miRNAs and their influence on transcriptomic signatures in prostate cancer by Fahad M. Aldakheel, Hadeel Alnajran, Ayesha Mateen, Shatha A. Alduraywish, Mohammed S. Alqahtani, Rabbani Syed

“…This analysis uncovered their substantial impact on critical biological processes and molecular functions, such as cyclin-dependent protein kinase activity, mitotic DNA damage checkpoint signalling, stress-activated MAPK cascade, regulation of extrinsic apoptotic signalling pathway, and positive regulation of cell adhesion. …”
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c-FLIP and microRNA 708-5p gene expression in newly diagnosed and chemotherapy in acute myeloid leukemia of Iraqi patient by Jaafar Sadiq Jaafar, Hiba Muneer Abdual Hassan Al-Kafagi, Israa Hussein Hamzah

“…The methods employed in the analysis include total RNA extraction, complementary cDNA synthesis, and quantitative real-time polymerase chain reaction (PCR) were used to evaluate the gene expression of cFLIP and miRNA-708-5p. …”
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An integrated method for taxonomic identif ication of microorganisms by Yu. E. Uvarova, A. V. Bryanskaya, A. S. Rozanov, V. N. Shlyakhtun, E. A. Demidov, K. V. Starostin, T. N. Goryachkovskaya, S. V. Shekhovtsov, N. M. Slynko, S. E. Peltek

“…For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. …”
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Comprehensive analysis of full genome sequence and Bd-milRNA/target mRNAs to discover the mechanism of hypovirulence in Botryosphaeria dothidea strains on pear infection with BdCV1... by Wangcheng Hu, Hui Luo, Yuekun Yang, Qiong Wang, Ni Hong, Guoping Wang, Aiming Wang, Liping Wang

“…It has high repeat sequence with 9.3% and DNA methylation existence in the genome. The 46.34 Mb genomes contained 14,091 predicted genes, which of 13,135 were annotated. …”
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