乙肝病毒X蛋白对肝癌细胞组蛋白H3K4甲基化修饰的影响及机制

“…【方法】构建稳定表达HBx基因的HepG2-hbx及载体对照细胞系HepG2-vc,同时培养用于阳性对照的肝癌细胞系HepG2.2.15及阴性对照细胞系HepG2;用酶标法检测各组细胞组蛋白H3K4位点甲基转移酶活性,比较HBx对H3K4位点甲基转移酶活性的影响;qRT-PCR检测各组细胞中H3K4位点特异性甲基转移酶SMYD3 ( SET and MYND domain containing3 )基因转录水平变化;Western-blot 检测SMYD3蛋白表达水平变化;免疫组化方法检测84例肝癌组织(包含:HbsAg(+)58例,HbsAg(-)26例)中SMYD3与组蛋白H3K4me3表达情况并对结果进行统计分析?…”
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Beyond 0-1: The 1-N Principle and Fast Validation of N-Sorter Sorting Networks by Robert B. Kent, Marios S. Pattichis

“…The well-known 0&#x2013;1 principle for traditional data-oblivious sorting networks states that a network with L inputs can be fully validated with an input vector set consisting of all <inline-formula> <tex-math notation="LaTeX">$2^{L}$ </tex-math></inline-formula> unique vectors containing only the values 0 and 1 in each vector&#x2019;s L inputs. …”
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Validation of a paediatric sepsis screening tool to identify children with sepsis in the emergency department: a statewide prospective cohort study in Queensland, Australia by Paula Lister, Kristen Gibbons, Luregn J Schlapbach, Adam Irwin, Michael Rice, Sainath Raman, Amanda Harley, Patricia Gilholm

“…We assessed the performance of the paediatric sepsis screening tool using stepwise multiple logistic regression analyses with 10-fold cross-validation and evaluated the final model at defined risk thresholds.Setting Twelve emergency departments (EDs) in Queensland, Australia.Participants 3473 children screened for sepsis, of which 523 (15.1%) were diagnosed with sepsis.Interventions A 32-item paediatric sepsis screening tool including rapidly available information from triage, risk factors and targeted physical examination.Primary outcome measure Senior medical officer-diagnosed sepsis combined with the administration of intravenous antibiotics in the ED.Results The 32-item paediatric sepsis screening tool had good predictive performance (area under the receiver operating characteristic curve (AUC) 0.80, 95% CI 0.78 to 0.82). A simplified tool containing 16 of 32 criteria had comparable performance and retained an AUC of 0.80 (95% CI 0.78 to 0.82). …”
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Beneficial Effects of <i>In Vitro</i> Reconstructed Human Gut Microbiota by Ginseng Extract Fermentation on Intestinal Cell Lines by Margherita Finazzi, Federica Bovio, Matilde Forcella, Marina Lasagni, Paola Fusi, Patrizia Di Gennaro

“…Among the natural compounds that we can introduce by the diet, <i>Panax ginseng</i> extract is one of the most utilized since it contains a vast number of bioactive molecules such as phenolic acids, flavonoids, and polysaccharides that have been shown to possess antioxidant, anti-ageing, anti-cancer, and immunomodulatory activity. …”
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Entropy generation and heat convection analysis of second-grade viscoelastic nanofluid flow in a tilted lid-driven square enclosure: A finite difference approach by Anil Ahlawat, Mukesh Kumar Sharma, K. Loganathan

“…Therefore, present work numerically analyzes the convection of heat and entropy production for a lid driven flow in a tilted square enclosure containing viscoelastic nanofluid exposed to a static magnetic field with a magnitude denoted as ‘B0’. …”
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Sneak Peek: Food, Waste, and Packaging Characteristics of South Australian School Children’s Lunchboxes by Neha Kishan Lalchandani, Clare Hume, Lynne Giles, Shona Crabb, Jo Hendrikx, Caroline Miller

“…Lunchboxes of preschool children contained more fruits (92% vs 65%; χ2(1)=73.3, P<0.01), vegetables (36% vs 16%; χ2(1)=34.0, P<0.01), and dairy items (45% vs 19%; χ2(1)=53.6, P<0.01), compared to lunchboxes of primary school children. …”
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Assessing Nonlinear Dynamics and Trends in Precipitation by Ensemble Empirical Mode Decomposition (EEMD) and Fractal Approach in Benin Republic (West Africa) by Médard Noukpo Agbazo, Gabin Koto N’Gobi, Eric Alamou, Basile Kounouhewa, Abel Afouda

“…Climate dynamics and trends have significant environmental and socioeconomic impacts; however, in the Benin Republic, they are generally studied with diverse statistical methods ignoring the nonstationarity, nonlinearity, and self-similarity characteristics contained in precipitation time series. This can lead to erroneous conclusions and an unclear understanding of climatic dynamics. …”
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Identification of tacrolimus-related genes in familial combined hyperlipidemia and development of a diagnostic model using bioinformatics analysis by Yuan Xu, Hongfei He, Haiyang Li

“…Methods: Dataset GSE1010 containing information on patients diagnosed with FCHL was obtained from the Gene Expression Omnibus (GEO), and an ensemble of tacrolimus-related genes (TRGs) was retrieved from the GeneCards, STITCH, and Molecular Signatures Database databases. …”
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Development of a marker panel for genotyping of domestic soybean cultivars for genes controlling the duration of vegetation and response to photoperiod by R. N. Perfil’ev, A. B. Shcherban, E. A. Salina

“…The studied cultivars contained the most common alleles of the GmFT2a and GmFT5a genes, with the exception of the ‘Cassidi’ cultivar having a rare dominant allele GmFT5a-H4. …”
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Engineered testbeds for investigating syringe injection performance in human factors studies by Andrew Pack, Sabrina Su, Frank A. Drews, Amrish Chourasia, Kevin Cluff, Joy Guerrieri, Jungkyu Kim

“…Results: Results from data collection using a syringe pump with solutions of 5, 10, 15, and 20 centipoise (cP) revealed a polynomial relationship between viscosity and force, contrary to the expected linear relationship. This unexpected pattern likely resulted from complex fluid dynamics in the 27-gauge needle. …”
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A New Strain of Preponderant Amphitriploid Carassius Clone Juvenile With Integrated Genomes Partly From White Crucian Carp (C. auratus cuvieri) Requires Low Dietary Protein by Xing Wang, Dong Han, Junyan Jin, Xiaoming Zhu, Haokun Liu, Zhimin Zhang, Yunxia Yang, Shouqi Xie

“…Seven groups of fish (body weight: 9.73 ± 0.03 g) were fed with seven isolipidic and isocarbohydrate diets containing 21.38%, 25.82%, 27.94%, 31.36%, 34.23%, 37.87%, and 40.70% crude protein (P21, P24, P27, P30, P33, P36, and P39), respectively. …”
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Preliminary Exploration of Natural Polyphenols and/or Herbal Mix Replacing Sodium Nitrate to Improve the Storage Quality of Pork Sausage by Angeliki-Niki Kaloudi, Agori Karageorgou, Michael Goliomytis, Panagiotis Simitzis

“…Five batches of sausages were manufactured using the same recipe and raw materials; the control, without sodium nitrate and natural preservatives (CON), and four groups supplemented with sodium nitrate at 0.015% (SN), olive polyphenols at 0.2% (POL), an herbal mix containing rosemary, savory, camelia, thyme, lemon balm and turmeric at 0.2% (NM), or the concomitant addition of olive polyphenols (0.1%) and the herbal mix (0.1%) (POL + NM). …”
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The Influence of Rice Types and Boiling Time on Glycemic Index: An In Vivo Evaluation Using the ISO 2010 Method by Anna Vîrlan, Lidia Coșciug, Dinu Țurcanu, Rodica Siminiuc

“…Ten healthy participants (mean age 25 years, body mass index (BMI) 23.0 ± 1.6 kg/m²) consumed rice samples containing 50 g of available carbohydrates, prepared under controlled boiling conditions. …”
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Analysis of the lncRNA-Associated Competing Endogenous RNA (ceRNA) Network for Tendinopathy by Bing-Zhe Huang, Yang Jing-Jing, Xiao-Ming Dong, null Zhuan Zhong, null Xiao-Ning Liu

“…Genes in MCODE 2 (such as TPM4) were significantly involved in cardiomyopathy, and genes in MCODE 4 (such as COL4A3 and COL4A4) were involved in focal adhesion, ECM-receptor interaction, and PI3K-Akt signaling pathway. The ceRNA network contained 7 lncRNAs (MIR133A1HG, LINC01405, PRKCQ-AS1, C10orf71-AS1, MBNL1-AS1, HOTAIRM1, and DNM3OS), 63 mRNAs, and 41 miRNAs. …”
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The relationship between college students’ Suicidal ideation and rejection sensitivity: a network analysis by Yang He, Tianqi Yang, Chunyan He, Yuanbei Zhang, Huilin Yang, Shen Huang, Jianjun Liu, Xihong Li, Tao Xu, Shengjun Wu

“…The rejection sensitivity and suicide ideation network contained 23 cross-community edges, with the strongest one being the connection between S3 “Despair” and R17 “No confidence”. …”
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POTENTIAL OF THE KANTIAN NOTION OF SOCIAL JUSTICE by Z. Kieliszek

“…This paper aims to show how the views of Kant persist in the modern debate on social justice and to outline the practical and political potential contained in his understanding of a just state system and international justice. …”
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Efficacy of Novel Noncontrast Cardiac Magnetic Resonance Methods in Indicating Fibrosis in Hypertrophic Cardiomyopathy by Maedeh Sharifian, Nahid Rezaeian, Sanaz Asadian, Ali Mohammadzadeh, Ali Nahardani, Kianosh Kasani, Yaser Toloueitabar, Ali Mohammad Farahmand, Leila Hosseini

“…FT-CMR, especially regional circumferential strain, can reliably show fibrosis-containing segments in HCM. Further, DWI can function as an efficient qualitative method for the estimation of the fibrosis extent in HCM.…”
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shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells by Qun Cheng, Shu Zhen Jiang, Li Bo Huang, Wei Ren Yang

“…Objective This study aims to verify the protective effect of the Kelch-like ECH-associated protein1 (Keap1)–nuclear factor erythroid 2–related factor 2 (Nrf2) signaling pathways by studying the effect of plasmids containing Nrf2–small hairpin RNA (shRNA) interference down-regulation of Nrf2 on zearalenone (ZEA)–induced intestinal porcine epithelial cells (IPEC-J2) oxidative stress. …”
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Analisis Sentimen Maskapai Penerbangan Menggunakan Metode Naive Bayes dan Seleksi Fitur Information Gain by Arif Bijaksana Putra Negara, Hafiz Muhardi, Indira Melinda Putri

“…Other visitors who read the reviews can get a more objective picture of airlines. The user reviews contained on the pegipegi.com website are currently very large so this makes it difficult and time consuming to read in its entirety. …”
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The influence of combinations of alien translocations on <i>in vitro</i> androgenesis in spring common wheat (<i>Triticum aestivum</i> L.) by E. M. Timonova, I. G. Adonina, E. A. Salina

“…The aim of this study was to develop a set of DH wheat lines containing a wheat-alien translocation in the genome and study the effect of alien translocations on androgenesis of anther culture in such lines.Materials and methods. …”
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