Wharton’s Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 23... by Kevin Dzobo, Matjaz Vogelsang, Nicholas E. Thomford, Collet Dandara, Karlien Kallmeyer, Michael S. Pepper, M. Iqbal Parker

“…Vascular endothelial growth factor, Akt, and Nodal pathways were downregulated in cancer cells cocultured with WJ-MSCs. We also demonstrate that WJ-MSCs effects on cancer cells appear to be short-lived whilst the fd-ECM effect is long-lived. …”
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Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response by Yaoqin Hu, Chaojin Qin, Guoping Zheng, Dengming Lai, Huikang Tao, Yan Zhang, Guanguan Qiu, Menghua Ge, Lanfang Huang, Lina Chen, Baoli Cheng, Qiang Shu, Jianguo Xu

“…Mouse peritoneal macrophages were cocultured with ASCs in a Transwell system for 2 days to educate macrophages. …”
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Interesting Cytokine Profile Caused by Clinical Strains of Pseudomonas aeruginosa MDR Carrying the exoU Gene by Nallely S. Badillo-Larios, Edgar Alejandro Turrubiartes-Martínez, Esther Layseca-Espinosa, Roberto González-Amaro, Luis Fernando Pérez-González, Perla Niño-Moreno

“…Finally, the strains were cocultured with PBMC, and cell survival and the cytokines IL-1β, IL-6, IL-10, IL-8, and TNF-α were quantified using flow cytometry. …”
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Human Cardiac Fibroblast Number and Activation State Modulate Electromechanical Function of hiPSC-Cardiomyocytes in Engineered Myocardium by Cassady E. Rupert, Tae Yun Kim, Bum-Rak Choi, Kareen L. K. Coulombe

“…The current study evaluates the functional effects of coculturing adult human cardiac fibroblasts (hCFs) in 3D engineered tissues on excitation and contraction with the goal of recapitulating healthy, nonarrhythmogenic myocardium in vitro. …”
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The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis by Lili Li, Bo Bao, Xingxing Chai, Xiaoyu Chen, Xiaohua Su, Shixiu Feng, Xiaohui Zhu

“…This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. GES-1 cells were cocultured with HPSS1 at MOI = 100 : 1 and treated with different concentrations of CN (100 and 200 μg/ml). …”
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Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/... by Zhaoxia Yu, Ju Liu, Qiqi Fan, Jun Yu, Xiaoting Ren, Xiaobin Wang

“…Then, BM-MSCs and EV were isolated and identified, and, EV were cocultured with HCC cells for further functional assays. …”
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BMSCs Regulate Astrocytes through TSG-6 to Protect the Blood-Brain Barrier after Subarachnoid Hemorrhage by Yilv Wan, Min Song, Xun Xie, Zhen Chen, Ziyun Gao, Xiang Wu, Rui Huang, Min Chen

“…BMSCs were transfected with TSG-6 inhibitor plasmid and cocultured with astrocytes. Intravenous transplantation of BMSCs was utilized to treat SAH rats. …”
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Umbilical Cord Tissue-Derived Mesenchymal Stem Cells Induce T Lymphocyte Apoptosis and Cell Cycle Arrest by Expression of Indoleamine 2, 3-Dioxygenase by Xiuying Li, Zhuo Xu, Jinping Bai, Shuyuan Yang, Shuli Zhao, Yingjie Zhang, Xiaodong Chen, Yimin Wang

“…IDO was previously reported to induce T lymphocyte apoptosis and cell cycle arrest in S phase. When cocultured with hUC-MSCs, T lymphocyte expression of caspase 3 was significantly increased, while Bcl2 and CDK4 mRNA expression decreased dramatically. …”
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Neutrophils Are Dysregulated in Patients with Hereditary Angioedema Types I and II in a Symptom-Free Period by Tereza Grymova, Marcela Vlkova, Premysl Soucek, Roman Hakl, Jana Nechvatalova, Peter Slanina, Julie Stichova, Jiri Litzman, Tomas Freiberger

“…As CD274 mediates inhibitory signals to different immune cells, neutrophils were cocultured with T-cells/PBMC. The decrease in CD25+ and IFN-γ+ T-cell/PBMC ratio in patients indicated the patients’ neutrophil suppressive functions. …”
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The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles by Selin Yildirim, Noushin Zibandeh, Deniz Genc, Elif Merve Ozcan, Kamil Goker, Tunc Akkoc

“…Lymphocytes were isolated from healthy peripheral venous blood. Lymphocytes were cocultured with stem cells in the presence and absence of IFN-γ and stimulated with anti-CD2, anti-CD3, and anti-CD28 for 3 days. …”
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Treatment of Radiation Bone Injury with Transplanted hUCB-MSCs via Wnt/β-Catenin by Yufeng Zhang, Huaxin Deng, Zhiqiang Yang, Zhe Chen, Sheng Zhang, Yufan Zhu, Min Yang, Houcheng Zhong, Fuling Zhou, Yuanlong Xie, Lin Cai

“…Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) were cocultured with irradiated osteoblasts to examine their therapeutic effects and mechanisms on osteoblast injury. …”
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ANKRD22 participates in the proinflammatory activities of macrophages in the colon cancer tumor microenvironment by Xiaoying Wang, Keqing Yang, Bin Yang, Rui Wang, Yongliang Zhu, Tianhui Pan

“…A significant decrease in ANKRD22 expression in macrophages cocultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues was observed. …”
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The Therapeutic Effect of Cytokine-Induced Killer Cells on Pancreatic Cancer Enhanced by Dendritic Cells Pulsed with K-Ras Mutant Peptide by Guang Tan, Xin Zhang, Hongbo Feng, Haifeng Luo, Zhongyu Wang

“…This study is to investigate the role of the CIKs cocultured with K-ras-DCs in killing of pancreatic cancer cell lines, PANC-1 (K-ras+) and SW1990 (K-ras−). …”
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MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke by Xiaoqing Ma, Ho Jun Yun, Kenneth Elkin, Yunliang Guo, Yuchuan Ding, Guangwen Li

“…Additionally, hCMEC/D3 cells were subjected to oxygen–glucose deprivation (OGD) and cocultured with HL-60 cells. Results. miR-29b levels in neutrophils were found to be significantly lower in stroke patients compared with healthy controls, which may indicate its high diagnostic sensitivity and specificity for stroke. …”
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Photobiomodulation Can Enhance Stem Cell Viability in Cochlea with Auditory Neuropathy but Does Not Restore Hearing by So-Young Chang, Eunjeong Kim, Nathaniel T. Carpena, Jae-Hun Lee, Doo Hee Kim, Min Young Lee

“…To confirm the engraftment of stem cells in vitro, cochlear explants were treated with kanamycin (KM) to mimic nerve damage and then cocultured with GFP-mESC. GFP-mESCs were observed to have attached and integrated into the explanted samples. …”
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Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis by Rongxiang Wu, Zhan Su, Le Zhao, Ruifeng Pei, Yiren Ding, Deqiang Li, Shuo Zhu, Lu Xu, Wei Zhao, Wuyuan Zhou

“…NEAT1 competitively bound to miR-491-5p to elevate Snail expression and diminish SOCS3 expression. PCa cells were cocultured with EVs extracted from ADSCs, followed by assessment of malignant phenotypes, tumorigenesis, and gemcitabine resistance of PCa cells using gain- or loss-of-function experiments. …”
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IL-33/ST2 Activation Is involved in Ro60-Regulated Photosensitivity in Cutaneous Lupus Erythematosus by Yitian Song, Fangzhi Wei, Ying Liu, Feng Han, Lihui Ma, Yanping Zhuang, Chengdan Pan, Zhandong Jia, Aimin Gong

“…Keratinocytes were cocultured with J774.2 macrophages and stimulated with IL-33 for analysis of chemostasis. …”
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miR-3960 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Inactivates SDC1/Wnt/β-Catenin Axis to Relieve Chondrocyte Injury in Osteoarthritis by Targeting PHLDA2 by Peng Ye, Zhanhu Mi, Daihao Wei, Pengcheng Gao, Mei Ma, Haibo Yang

“…EVs were isolated from MSCs and cocultured with IL-1β-induced OA chondrocytes. Afterwards, cellular biological behaviors and levels of extracellular matrix- (ECM-) related protein anabolic markers (collagen II and aggrecan), catabolic markers (MMP13 and ADAMTS5), and inflammatory factors (IL-6 and TNF-α) in chondrocytes were assayed upon miR-3960 and/or PHLDA2 gain- or loss-of-function. …”
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Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway by Min Liu, Rui Chen, Yunxuan Xu, Jiawen Zheng, Min Wang, Ping Wang

“…Then, cellular senescence indicators were evaluated after high glucose (25 mM) treatment of cultured PDLSCs. PDLSC-Exos were cocultured with senescent PDLSCs to further explore the role of PDLSC-Exos in cellular senescence and determine the differences in cellular oxidative stress levels after PDLSC-Exo treatment. …”
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Acute Leukemia Induces Senescence and Impaired Osteogenic Differentiation in Mesenchymal Stem Cells Endowing Leukemic Cells with Functional Advantages by Ximena Bonilla, Natalia-Del Pilar Vanegas, Jean Paul Vernot

“…To deepen on this topic, we have used an in vitro model of the leukemic niche (LN) by coculturing MSC with an acute lymphocytic leukemia cell line (REH) and proceeded to evaluate MSC characteristics and functions. …”
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