Mesenchymal Stem Cells Pretreated with HGF and FGF4 Can Reduce Liver Fibrosis in Mice by Sulaiman Shams, Sadia Mohsin, Ghazanfar Ali Nasir, Mohsin Khan, Shaheen N. Khan

“…In vitro we gave CCl4 injury to mouse hepatocytes and cocultured it with untreated and cytokines pretreated MSCs. …”
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Understanding Cardiovascular Events With JAK Inhibitors: Tofacitinib Reduces Synovial and Vascular Inflammation but not the Prothrombotic Effects of Inflammatory Cytokines on Endot... by Aliki Zavoriti, Pierre Miossec

“…In cocultures with ECs, tofacitinib reduced further IL‐6 and IL‐8 production (both P < 0.001). …”
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Upregulated Expression of Indoleamine 2, 3-Dioxygenase in Primary Breast Cancer Correlates with Increase of Infiltrated Regulatory T Cells In Situ and Lymph Node Metastasis by Jinpu Yu, Jingyan Sun, Shizhen Emily Wang, Hui Li, Shui Cao, Yizi Cong, Juntian Liu, Xiubao Ren

“…We further demonstrated that CD4+CD25+CD127− Tregs could be amplified by coculturing CD3+ T cells with IDO/CHO cells in vitro which displayed increasing Foxp3 expression both at mRNA and protein levels. …”
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Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids by Bruno Corrêa Bellagamba, Patrícia Bencke Grudzinski, Pedro Bins Ely, Paulo de Jesus Hartmann Nader, Nance Beyer Nardi, Lindolfo da Silva Meirelles

“…Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. …”
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Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis by Haisu Dai, Yan Jiang, Zhipeng Liu, Xingxing Su, Yishi Yang, Zhiyu Chen

“…Moreover, angiogenesis-related signaling in HUVECs was activated when HUVECs were cocultured with PC cells overexpressing PUM1. Nevertheless, PC cells silenced with PUM1 had the opposite effect. …”
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Tumor-associated macrophages promote bladder cancer metastasis through the CCL20-CCR6 axis by Ryunosuke Nakagawa, Kouji Izumi, Kaoru Hiratsuka, Takahiro Inaba, Yoshiki Koketsu, Ren Toriumi, Shuhei Aoyama, Taiki Kamijima, Hiroshi Kano, Tomoyuki Makino, Renato Naito, Suguru Kadomoto, Hiroaki Iwamoto, Hiroshi Yaegashi, Shohei Kawaguchi, Takahiro Nohara, Kazuyoshi Shigehara, Hiroki Nakata, Wen-Jye Lin, Atsushi Mizokami

“…We investigated the mechanisms of interaction between bladder cancer (BC) cells and tumor-associated macrophages (TAMs). Coculturing BC cell lines (UMUC3 and T24) with macrophage-like cells differentiated from THP-1 into M2-like TAMs revealed a decrease in Cluster of Differentiation (CD) 68 expression and an increase in CD206 expression. …”
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Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis by Zehui Yang, Yingying Chen, Qiang Zhang, Xiaodong Chen, Ze Deng

“…The chemotactic activity, toxicity, and phagocytosis of RAW 264.7 cocultured with different concentrations of MOMP were determined by Transwell, CCK-8, and neutral red uptake assays, respectively. …”
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The SIX1/LDHA Axis Promotes Lactate Accumulation and Leads to NK Cell Dysfunction in Pancreatic Cancer by Wanli Ge, Lingdong Meng, Shouji Cao, Chaoqun Hou, Xiaole Zhu, Dongya Huang, Qiang Li, Yunpeng Peng, Kuirong Jiang

“…PC cells and NK cells were cocultured to study the effect of SIX1 overexpression in PC cells on NK cell function. …”
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Blockade of 4-1BB and 4-1BBL Interaction Reduces Obesity-Induced Skeletal Muscle Inflammation by Ngoc Hoan Le, Chu-Sook Kim, Thai Hien Tu, Hye-Sun Choi, Byung-Sam Kim, Teruo Kawada, Tsuyoshi Goto, Taesun Park, Jung Han Yoon Park, Rina Yu

“…Conversely, disruption of the interaction between 4-1BB and its ligand (4-1BBL) with blocking antibody decreased the release of inflammatory cytokines from cocultured muscle cells/macrophages. Moreover, deficiency of 4-1BB markedly reduced macrophage infiltration and inflammatory cytokine production in the skeletal muscle of mice fed a high-fat diet. …”
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Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway by Qi-Ming Pang, Rui Yang, Meng Zhang, Wang-Hui Zou, Nan-Nan Qian, Qi-Jing Xu, Hui Chen, Jia-Chen Peng, Xiao-Ping Luo, Qian Zhang, Tao Zhang

“…Furthermore, western blotting and immunofluorescence assays demonstrated that JAK1/STAT3 signaling was significantly upregulated in macrophages cocultured with PBMSCs or P-CM, accompanied by an increase in the M2 biomarker CD206 and a decrease in the M1 biomarker CD86. …”
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Mesenchymal Stem Cells May Alleviate the Intervertebral Disc Degeneration by Reducing the Oxidative Stress in Nucleus Pulposus Cells by Yongzhao Zhao, Qian Xiang, Yunzhong Cheng, Jialiang Lin, Shuai Jiang, Weishi Li

“…The aim of this study was to determine the vital role of oxidative stress-related differentially expressed genes (OSRDEGs) in degenerative NPCs cocultured with mesenchymal stem cells (MSCs). Methods. …”
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Notch1 Signaling Regulates the Th17/Treg Immune Imbalance in Patients with Psoriasis Vulgaris by Lei Ma, HaiBo Xue, Tianqin Gao, MeiLan Gao, YuJie Zhang

“…DAPT treatment resulted in the obvious downregulation of Th17 cell percentage in cocultured CD4+ T cells, RORγt and IL-17 mRNA levels, and IL-17 concentration in cell-free supernatant from cocultured CD4+ T cells of PV patients in a dose-dependent manner, while there was no significant influence on Treg cell percentage, Foxp3, and IL-10 expression, therefore leading to the recovery of Th17/Treg immune imbalance. …”
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Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2 by Lihua Wang, Lei Yang, Tingting Zhuang, Xiuqing Shi

“…Furthermore, the downregulation of ROBO1 and SRGAP2 in cocultured HUVECs was also reduced after additional treatment with GW4869. …”
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Depletion and Reversal of Hepatocellular Carcinoma Inducing CTL through ER Stress-Dependent PERK-CHOP Signaling Pathway by Mengnan Guo, Wei Wang, Wen Bai, Zekun Bai, Weixi Chen, Yali Su, Jinghua Wu

“…The expression of GRP78, PD-1, and TIM-3 was detected by western blotting in CTLL-2 cocultured with Hepa1-6 cells. Moreover, perforin (PRF1) and granzyme B (GzmB) protein levels and ER morphology were examined by ELISA and TEM, respectively. …”
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Novel treatment strategy targeting interleukin-6 induced by cancer associated fibroblasts for peritoneal metastasis of gastric cancer by Ema Mitsui, Satoru Kikuchi, Tomohiro Okura, Hiroshi Tazawa, Yuta Une, Noriyuki Nishiwaki, Shinji Kuroda, Kazuhiro Noma, Shunsuke Kagawa, Toshiaki Ohara, Junko Ohtsuka, Rieko Ohki, Toshiyoshi Fujiwara

“…In vitro, differentiation of fibroblasts into CAFs and chemoresistance were observed in GC cells cocultured with fibroblasts. Anti-IL-6R Ab inhibited the progression of PM in GC cells cocultured with fibroblasts in the orthotopic mouse model but could not inhibit the progression of PM consisting of GC cells alone. …”
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SDF-1α Facilitates Mesenchymal Stem Cells to Induce Regulatory B Cell Differentiation from Patients with Immune Thrombocytopenia by Zhe Chen, Shufen Zhou, Jianyun Li, Hui Li, Can Huang, Qin Guo, Tiantian Zhang, Bingya Yang, Chuanqing Tu, Chengshan Guo

“…The proportion of Bregs and the IL-10 secretion could be further promoted by the UC-MSCs treated with 0.1 μg/mL SDF-1α, which could also promote the miRNA-133 expression of UC-MSCs in an exosome-dependent manner; moreover, while the UC-MSCs were transfected with the miR-133 inhibitor, the proportion of induced Bregs decreased obviously when cocultured with peripheral blood mononuclear cells (PBMCs) of ITP. …”
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lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis by Shan-zheng Wang, Jun Jia, Chang-hong Chen

“…However, miR-141-5p silencing or cocultured with ADSCs-Exos attenuated these effects. …”
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Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell–Cell Fusion and Immune Activation by Yao Wang, Xunlei Pang, Ruirui Li, Jiuzhou Chen, Chen Wen, Huihuang Zhu, Tingyu Long, Jianjie Li, Lijun Zheng, Youcai Deng, Junnian Zheng, Bo Xu

“…Methods: We constructed a fusogenic protein, fusion-associated small transmembrane (FAST) p14 of reptilian reovirus, into cancer cells and mesenchymal stem cells (MSCs), which cocultured with various colon cancer cells and melenoma cells to validate its ability to induce cell fusion and syncytia formation. …”
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M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin by Daniel Ngabire, Irvine Niyonizigiye, Maheshkumar Prakash Patil, Yeong-Ae Seong, Yong Bae Seo, Gun-Do Kim

“…THP-1 cells were polarized using recombinant human IL-4, then were cocultured with AGS cells treated with fluorouracil. …”
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Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs by Lifang Zhang, Xinping Li, Congying Shi, Gaoying Ran, Yuting Peng, Shuguang Zeng, Yan He

“…Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. …”
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