Hurdle Approach to Simulate Corn Wet Milling Inactivation of Undesirable Microorganisms: A Pilot Scale Microbial Challenge Study Using Salmonella Surrogate Enterococcus faecium by Erin Kealey, Madeline Shick, Minho Kim, Ruben Chavez, Gordon Shetley, David Stenger, Kirk A. Perreau, Allison Cooke, Cecil Barnett-Neefs, Matthew J. Stasiewicz

“…Finished products are historically safe, with intrinsic properties such as low water activity inhibiting microbial growth. Corn processors could use quantified levels of reduction in this study of Salmonella surrogate Enterococcus faecium (E. faecium) to update their food safety plans. …”
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An inner membrane complex protein IMC1g in Plasmodium berghei is involved in asexual stage schizogony and parasite transmission by Yinjie Liu, Shitong Cheng, Gang He, Dawei He, Duo Wang, Sicong Wang, Lumeng Chen, Liying Zhu, Yonghui Feng, Liwang Cui, Yaming Cao, Xiaotong Zhu

“…Localization of PbIMC1g to the IMC was dependent on its IMCp domain, while its C-terminus and palmitoylation sites were required for the full efficiency of proper IMC targeting. …”
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Histamine N-methyltransferase (HNMT) as a potential auxiliary biomarker for predicting adaptability to anti-HER2 drug treatment in breast cancer patients by Tzu-Chun Cheng, Mien-Chie Hung, Lu-Hai Wang, Shih-Hsin Tu, Chih-Hsiung Wu, Yun Yen, Chi-Long Chen, Jacqueline Whang-Peng, Wen-Jui Lee, You-Cheng Liao, Yu-Ching Lee, Min-Hsiung Pan, Hui-Kuan Lin, Huey-En Tzeng, Peixuan Guo, Cheng-Ying Chu, Li-Ching Chen, Yuan-Soon Ho

“…Furthermore, we evaluated the influence of HNMT activity on the binding of anti-HER2 antibodies to their targets through flow cytometry. …”
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A multicenter analysis to identify the risk factors for stroke recurrence and mortality within 1 year by Eman A. Alraddadi, Eman A. Alraddadi, Hashim F. Alotaibi, Hashim F. Alotaibi, Yasser Alatawi, Ahmed Aljabri, Ahmed A. Alghamdi, Ahmed A. Alghamdi, Fahad Alturki, Fahad Alturki, Faisal F. Alamri, Faisal F. Alamri

“…The multivariable analysis identified risk factors for stroke recurrence including history of previous stroke (HR = 3.65, 95% CI:2.28–5.99, p = 0.0001), tissue plasminogen activator (tPA) treatment (HR = 2.84, 95% CI:1.57–4.86, p = 0.0003), seizure (HR = 1.96, 95% CI:1.14–3.22, p = 0.0105), and depression (HR = 2.26, 95% CI:1.33–3.69, p = 0.0016). …”
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The miR-1269a/PCDHGA9/CXCR4/β-catenin pathway promotes colorectal cancer invasion and metastasis by Haitao Mei, Qingshan Luo, Junyong Weng, Jialing Hao, Jinfeng Cai, Runkai Zhou, Ce Bian, Yingzi Ye, Shengzheng Luo, Yugang Wen

“…The low level of PCDHGA9 activates the Wnt/β-catenin pathway by releasing its interaction with HOXA1, promoting the expression of CXCR4, and causing invasion, migration, and EMT in CRC. …”
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A conserved element in the first intron of Cd4 has a lineage specific, TCR signal-responsive, canonical enhancer function that matches the timing of cell surface CD4 upregulation r... by Gregory A. Swan, Gregory A. Swan, Chika Fujii, Mia E. Guzynski, Sheridan M. Page, Isabelle V. Meyers, Yordan P. Penev, Sejiro Littleton, Sejiro Littleton, Adinda Azzahra, Christine Richardson, Sophia D. Sarafova, Sophia D. Sarafova

“…In vivo, NCE-sufficient transgenic mice exhibited upregulation of Cd4 reporter EGFP mRNA levels at the INT stage and a corresponding upregulation of EGFP fluorescence, whereas NCE-deficient mice showed a significant loss of Cd4 reporter EGFP mRNA and no detectable EGFP production in any post-selection thymocytes.DiscussionThis study demonstrates that the canonical enhancer function of coreNCE/E4m is essential for CD4 upregulation following positive selection. The NCE region, with its developmental-stage-specific activity and its known epigenetic regulatory capabilities, ensures faithful lineage commitment to the CD4 lineage.…”
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Characteristic alterations of gut microbiota and serum metabolites in patients with chronic tinnitus: a multi-omics analysis by Jiang Wang, Jia-Hui Xiang, Xu-Yuan Peng, Min Liu, Le-Jia Sun, Min Zhang, Li-Yuan Zhang, Zhi-Bin Chen, Zheng-Quan Tang, Lei Cheng

“…In serum metabolomic analysis, serum metabolic disturbances in tinnitus patients and these differential metabolites were enriched in pathways of neuroinflammation, neurotransmitter activity, and synaptic function. The predictive models exhibited great diagnostic performance, achieving 0.94 (95% CI: 0.85–0.98) and 0.96 (95% CI: 0.86–0.99) in the test set. …”
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Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement by Liyuan Chen, Danqing He, Zixin Li, Shengjie Cui, Min Yu, Zimo Zhao, Yuetong Chen, Jiayi Song, Nan Jiang, Huajie Yu, Yan Liu

“…Endo180, an urokinase plasminogen activator receptor-associated protein, is a transmembrane receptor regulated collagen remodeling. …”
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The impact of war on people with type 2 diabetes in Ukraine: a survey studyResearch in context by Oksana Sulaieva, Viktoriia Yerokhovych, Sergii Zemskov, Iuliia Komisarenko, Vitalii Gurianov, Volodymyr Pankiv, Oleksandr Tovkai, Tetyana Yuzvenko, Violetta Yuzvenko, Andrii Tovkai, Zlatoslava Shaienko, Tetyana Falalyeyeva, Nadiya Skrypnyk, Taras Romaniv, Nadiya Pasyechko, Taras Krytskyy, Solomiia Danyliuk, Andrii Klantsa, Dmytro Krasnienkov, Oleksandr Gurbych, Nazarii Kobyliak

“…HbA1c levels demonstrated a time-dependent pattern of growth, reflecting the cumulative effect of war-related factors on T2D patients’ health. Witnesses of armed attacks and occupation aggravated the T2D course. …”
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A qualitative study exploring the acceptability of the TEXTPAD telehealth and virtual supervised exercise intervention and trial for patients with peripheral arterial disease livin... by Maddey Patterson, MPH, Letitia Sermin-Reed, MSc, James Prentis, MBBS, Arathi Radhakrishnan, MBBS, Eileen Kaner, PhD, Sandip Nandhra, MBBS, Maisie Rowland, BSc, Gabriel Cucato, PhD, Mackenzie Fong, PhD

“…Both TEXTPAD and control group participants were encouraged to engage in an unsupervised walking program and were provided with an activity tracker. This qualitative study aimed to explore participants' acceptance of the TEXTPAD and unsupervised walking programs and trial procedures. …”
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Supported biopsychosocial self-management for back-related leg pain: a randomized feasibility study integrating a whole person perspective by Brent Leininger, Roni Evans, Carol M. Greco, Linda Hanson, Craig Schulz, Michael Schneider, John Connett, Francis Keefe, Ronald M. Glick, Gert Bronfort

“…Self-management intervention providers delivered all required activities at 72% of visits. Providers also noted some challenges navigating the shared decision-making process and deciding what self-management tools to prioritize. …”
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Oral pre-exposure prophylaxis implementation in South Africa: a case study of USAID-supported programs by Jerome Wendoh Milimu, Lauren Parmley, Mahlodi Matjeng, Mathata Madibane, Mandisi Mabika, Jacques Livingston, Joseph Lawrence, Orapeleng Motlhaoleng, Hasina Subedar, Rethabile Tsekoa, Zandile Mthembu

Subjects: “…HIV prevention…”
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Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei by Maznah Alhesain, Ayman Alzu’bi, Ayman Alzu’bi, Ayman Alzu’bi, Niveditha Sankar, Niveditha Sankar, Charles Smith, Charles Smith, Janet Kerwin, Ross Laws, Susan Lindsay, Gavin J. Clowry

“…Disruption of CNTNAP2 activity and function could be hypothezised to have a variety of effects upon thalamic development.…”
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A qualitative study of stigma in patients with low anterior resection syndrome of rectal cancer (直肠癌低位前切除综合征患者病耻感的质性研究) by WEN Ruizhen (温瑞珍), CHEN Piao (陈飘), LI Yalan (李亚兰), CAO Aifang (曹爱芳), WANG Miaolan (王淼兰), ZHENG XiaoXuan (郑筱暄), PENG Hui (彭慧)

“…Theme 1: Stigma experiences, including feelings of shame, discrimination, and loneliness; Theme 2: Determinants of stigma, including disease factors and lack of public knowledge; Theme 3: Stigma sensing approaches, including avoiding social contact, concealing the condition, and actively coping. Conclusion Patients with LARS of rectal cancer have strong stigma. …”
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An exceptionally rare case of a diffuse midline glioma with concomitant H3.1 K27M and G34R mutations in the HIST1H3C (H3C3) gene by Zita Reisz, Rita Pereira, Smitha Nevis, Alan Mackay, Leena Bhaw, Yura Grabovska, Ross Laxton, Valeria Molinari, Anna Burford, Barnaby Clark, Cristina Bleil, Bassel Zebian, Erika Pace, Annette Weiser, Fernando Carceller, Lynley Marshall, Andrew King, Istvan Bodi, Safa Al-Sarraj, Chris Jones, Matthew Clarke

“…Histology showed diffusely infiltrating pleomorphic astrocytes, multinucleated cells, and conspicuous mitotic activity; the diagnosis was DMG, H3 K27-altered (immunohistochemistry: H3K27me3 loss, H3K27M positivity). …”
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Construction and Pathogenicity Analysis of a GFPuv-labeled Peanut Ralstonia solanacearum Strain by Jinling HUANG, Yushuang WU, Huiquan TANG, Minyi HUANG, Ruixue YANG, Qiang WANG, Yong YANG, Xiaodan TAN

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Prevalence and factors associated with severe fatigue 2 years into the COVID-19 pandemic: a cross-sectional population-based study in Geneva, Switzerland by Silvia Stringhini, Idris Guessous, C Martinez, A Flahault, S Hurst, N Vuilleumier, L Kaiser, K M Posfay-Barbe, O Zimmermann, S Stringhini, A Uppal, P Schaller, J Berthelot, D Pittet, H Baysson, Hélène Baysson, S Yerly, E Lorthe, Mayssam Nehme, Nick Pullen, María-Eugenia Zaballa, A Bal, Clément Romain Hugo Graindorge, Stephanie Schrempft, I Arm-Vernez, A S Azman, D Bachmann, J-F Balavoine, R P Barbe, A R Bouhet, G Bryand-Rumley, F Chappuis, P Collombet, S Coudurier-Boeuf, D S Courvoisier, C de Mestral, P D’ippolito, R Dubos, R Dumont, N El Merjani, N Francioli, C Graindorge, I Guessous, S Harnal, G Kathari, O Kherad, J Lamour, P Lescuyer, A G L’Huillier, A J Loizeau, S Mechoullam, L Metral-Boffod, M Nehme, N Noël, F Pennacchio, J Perez-Saez, G Poulain, C Pugin, N Pullen, V Richard, D Rochat, S Rouzinov, K Samir, H S Ramirez, E Satin, S Schrempft, C Semaani, S Testini, D Urrutia-Rivas, C Verolet, P Vetter, J Villers, G Violot, A Wisniak, M-E Zaballa

“…Objectives This study aims (1) to assess the prevalence of severe fatigue among the general population of Geneva, 2 years into the COVID-19 pandemic and (2) to identify pandemic and non-pandemic factors associated with severe fatigue.Design Cross-sectional population-based survey conducted in Spring 2022.Setting General adult population of Geneva, Switzerland.Participants 6870 adult participants, randomly selected from the general population, included in the Specchio-COVID-19 cohort study, were invited to answer an online health survey.Outcome and cofactor measure Prevalence of severe fatigue was measured by the Chalder Fatigue Questionnaire with a cut-off score≥4 out of 11. We assessed prevalence ratios of severe fatigue considering sociodemographic factors, health and behavioural characteristics (body mass index, depression, recent diagnosis of chronic disease or allergy, acute health event, smoking status, physical activity and sleep quality) and recent self-reported COVID-19 infections.Results A total of 4040 individuals participated (participation rate 59%, 58% were women, mean age 53.2 (SD=14.1 years)). …”
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Rat models of postintracerebral hemorrhage pneumonia induced by nasal inoculation with Klebsiella pneumoniae or intratracheal inoculation with LPS by Ruihua Wang, Changlian Gan, Rui Mao, Yang Chen, Ru Yan, Geng Li, Tianqin Xiong, Jianwen Guo

“…Bronchoalveolar lavage fluid was collected for leukocyte counting, and supernatant was prepared to measure MPO activity. Ileum was isolated for immunofluorescence staining for tight junction proteins ZO-1 and γδ TCRs/IL-17A and for Alcian blue–nuclear fast red staining of acidic mucins. …”
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Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling by Walid Mottawea, Basit Yousuf, Salma Sultan, Tamer Ahmed, JuDong Yeo, Nico Hüttmann, Yingxi Li, Nour Elhouda Bouhlel, Hebatoallah Hassan, Xu Zhang, Zoran Minic, Riadh Hammami

“…In this study, a multi-level analysis revealed presence of a diverse array of biologically active molecules encapsulated within MEVs, including neuroactive metabolites, such as arachidonyl-dopamine, gabapentin, glutamate, and N-acylethanolamines, and gamma-aminobutyric acid (GABA). …”
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Toxic effects and mechanisms of nanoplastics and sulfonamide antibiotics on Scenedesmus obliquus by Jianyuan Yang, Zhen Yang, Jing Wang, Yanpeng Liang, Honghu Zeng, Litang Qin, Xiaohong Song, Lingyun Mo

“…The exposure of two NPs and four SAs and their binary mixtures differently promoted or inhibited superoxide dismutase and catalase activities in algal cells to different degrees and resulted in elevated levels of malondialdehyde content, suggesting that oxidative stress led to significant inhibition of chlorophyll content, total protein content, and growth of algal cells. …”
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