Genetically Engineered Bacterial Ghosts as Vaccine Candidates Against <i>Klebsiella pneumoniae</i> Infection by Svetlana V. Dentovskaya, Anastasia S. Vagaiskaya, Alexandra S. Trunyakova, Alena S. Kartseva, Tatiana A. Ivashchenko, Vladimir N. Gerasimov, Mikhail E. Platonov, Victoria V. Firstova, Andrey P. Anisimov

“…<b>Methods:</b> To increase the efficiency of <i>Klebsiella pneumoniae</i> BG formation and, accordingly, to ensure maximum killing of bacteria, we exploited previously designed plasmids with the lysis gene <i>E</i> from bacteriophage φX174 or with holin–endolysin systems of λ or L-413C phages. Previously, this kit made it possible to generate bacterial cells of <i>Yersinia pestis</i> with varying degrees of hydrolysis and variable protective activity. …”
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Screening potential diagnostic biomarkers for PLA2R‑associated idiopathic membranous nephropathy by WGCNA analysis and LASSO algorithm by Jinxu Huang, Yaqing Huang, Xiaoling Zeng, Yuhong Zhang, Junneng Zhang, Qingchu Hong, Yongtiao Peng

“…Functional studies, prompted by RASD1 downregulation, revealed enhanced cell activity and reduced apoptosis upon RASD1 overexpression compared to the Serum + Ov-NC control. …”
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Appetite Suppression and Antiobesity Effect of a Botanical Composition Composed of Morus alba, Yerba mate, and Magnolia officinalis by Mesfin Yimam, Ping Jiao, Mei Hong, Lidia Brownell, Young-Chul Lee, Eu-Jin Hyun, Hyun-Jin Kim, Tae-Woo Kim, Jeong-Bum Nam, Mi-Ran Kim, Qi Jia

“…Background. Obesity and its comorbidities continue to challenge the world at an alarming rate. …”
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Association of Early and Late Contrast-Associated Acute Kidney Injury and Long-Term Mortality in Patients Undergoing Coronary Angiography by Zhubin Lun, Jin Liu, Liwei Liu, Jingjing Liang, Guanzhong Chen, Shiqun Chen, Bo Wang, Qiang Li, Haozhang Huang, Zhidong Huang, Danyuan Xu, Yunzhao Hu, Ning Tan, Jiyan Chen, Yong Liu, Jianfeng Ye

“…After adjusting for important clinical variables, early CA-AKI (HR = 1.33, 95% CI: 1.02–1.74, P=0.038) was significantly associated with mortality, while late CA-AKI (HR = 0.92, 95% CI: 0.65–1.31, P=0.633) was not. …”
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Prevalence and factors associated with neonatal sepsis in Mali: a cross-sectional study by Bienvenu Salim Camara, Alexandre Delamou, Haoua Dembele, Hamadoun Sangho, Birama Apho Ly, Sidikiba Sidibé, Alioune Camara, Cheick Sidya Sidibé, Alhassane Diallo, Abdoulaye Touré, Fatoumata Bintou Traoré, Elhadj Marouf Diallo, Fatimata Yaro, Mariam Traoré

“…Neonatal age &lt;7 days (AOR=2.79, 95% CI 1.59 to 4.89, p=0.000), low birth weight &lt;2500 g (AOR=2.88, 95% CI 1.41 to 5.86, p=0.003), Apgar score &lt;7 (AOR=4.03, 95% CI 3.09 to 5.24, p=0.000), mother with no education (AOR=2.24, 95% CI 1.15 to 4.33, p=0.02), maternal fever (AOR=2.31, 95% CI 1.53 to 3.53, p=0.000), prolonged rupture of membranes (AOR=1.87, 95% CI 1.01 to 3.54, p=0.04) and prolonged labour (AOR=2, 95% CI 1.03 to 3.88, p=0.04) were significantly associated with neonatal sepsis.Conclusion The prevalence of sepsis in Mali is still high. …”
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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

“…The N97 extract was most active with a 50% inhibitory concentration (IC50) of 33.35 µg/ml. …”
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Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail by Ewa Tomaszewska, Kamil Drabik, Kornel Kasperek, Piotr Dobrowolski, Monika Hułas-Stasiak, Renata Pyz-Łukasik, Waldemar Paszkiewicz, Monika Ziomek, Janine Donaldson, Katarzyna Dobrowolska, Artur Burmaczuk, Justyna Batkowska, Marcin B. Arciszewski, Siemowit Muszyński

“…Glucose and creatinine levels varied with Gln supplementation and between the two time points; enzyme activities (alanine transaminase, alkaline phosphatase, and gamma-glutamyl transpeptidase) were affected by both Gln dose and length of supplementation. …”
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A cross-sectional study on the correlation between internal cerebral vein asymmetry and hemorrhagic transformation following endovascular thrombectomy by Kunxin Lin, Kunxin Lin, Wenlong Zhao, Wenlong Zhao, Quanhong Wu, Quanhong Wu, Yiru Zheng, Yiru Zheng, Bo Yang, Ying Fu, Ying Fu, Ning Wang, Ning Wang, Ling Fang, Ling Fang

“…In multivariate logistic regression, ICV asymmetry (OR 3.809, 95% CI 1.582–9.171), baseline Alberta Stroke Program Early CT Score (OR 0.771, 95% CI 0.608–0.978), intravenous recombinant tissue plasminogen activator (OR 2.847, 95% CI 1.098–2.7.385), and poor collateral circulation (OR 3.998, 95% CI 1.572–10.169) were identified as independent risk factors of HT.ConclusionICV asymmetry, likely resulting from impaired autoregulation or tissue micro-perfusion hampering cerebral blood flow (CBF), is a novel radiological sign that independently predicts HT. …”
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Association between exposure to ambient air pollutants and metabolic syndrome in the vicinity of the Taklamakan Desert by Zhe Wang, Weidong Ji, Yushan Wang, Lin Li, Kai Wang, Hongze Liu, Yining Yang, Yi Zhou

“…A 10 μg/m³ increase in concentration was associated with the following respective odds ratios: PM1 (1.341, 95 % CI: 1.331, 1.351), PM2.5 (1.036, 95 % CI: 1.034, 1.037), PM10 (1.006, 95 % CI: 1.005, 1.007), O3 (1.385, 95 % CI: 1.374, 1.396), and CO (1.015,95 %, CI: 1.0147, 1.016). …”
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The Wayqecha Amazon Cloud Curtain Ecosystem Experiment: A new experimental method to manipulate fog water inputs in terrestrial systems by Daniel B. Metcalfe, Darcy F. Galiano Cabrera, Luis Miguel Alvarez Mayorga, Roxana Sacatuma Cruz, Daniela Corrales Alvarez, Blanca Rosa Espinoza Otazu, Walter Huaraca Huasco, Jimmy R. Chambi, Maria E. Ezquerra, Beisit L. Puma Vilca, Mark Mulligan, Matthew A. Vadeboncoeur, Heidi Asbjornsen, Paulo R. L. Bittencourt, Aline B. Horwath, David C. Bartholomew

“…Here, we present the design and performance of a novel experiment to actively manipulate low lying fog abundance in an old‐growth tropical montane cloud forest (TMCF) in Peru—the Wayqecha Amazon Cloud Curtain Ecosystem Experiment (WACCEE). …”
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Prevalence of psoriatic arthritis in Italy: insights from the multicentric MAPSI study by Teodora Serban, Giuseppina Tramontano, Monica Pendolino, Dario Roccatello, Oscar Massimiliano Epis, Florienzo Iannone, Ilenia De Andres, Marta Favero, Nicoletta Romeo, Guido Rovera, Leonardo Santo, Enrico Tirri, Alberto Bergamini, Roberta Foti, Carlotta Schenone, Gerolamo Bianchi

“…Initially perceived as relatively benign, PsA is now recognized for its chronic, progressive, and destructive nature, significantly impacting patients' quality of life, similar to Rheumatoid Arthritis (RA). …”
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C3G improves lipid droplet accumulation in the proximal tubules of high-fat diet-induced ORG mice by Yong-Ping Lu, Xiao-Hua Wang, Bin Xia, Hong-Wei Wu, Yan Lei, Kai-Wen Cai, Zi-Yan Deng, Chun Tang, Wei-Bin Bai, Ting Zhu, Zhi-Hua Zheng

“…Cyanidin-3-glucoside (C3G) has shown potential in regulating lipid metabolism and may offer reno-protective effects; however, its therapeutic efficacy and underlying mechanisms in ORG remain unclear. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Alongside cells, their released factors and extracellular vesicles (EVs), overall termed “secretome”, are actively sifted being envisioned as the main therapeutic actors. …”
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Bulk and single-cell transcriptome revealed the metabolic heterogeneity in human glioma by Yong Xiao, Mengjie Zhao, Ran Wang, Liang Liu, Chong Xiang, Taiping Li, Chunfa Qian, Hong Xiao, Hongyi Liu, Yuanjie Zou, Xianglong Tang, Kun Yang

“…Background: Emerging perspectives on tumor metabolism reveal its heterogeneity, a characteristic yet to be fully explored in gliomas. …”
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High concentrations of polyethylene microplastics restrain the growth of Cinnamomum camphora seedling by reducing soil water holding capacity by Run Yang, Jinping Wang, Huanying Fang, Jinwen Xia, Guomin Huang, Rongzhen Huang, Hong Zhang, Liqin Zhu, Lichao Zhang, Jihong Yuan

“…The accumulation of microplastics (MPs) in soils due to anthropogenic activities affects the growth and development of plants and thereby endangering the diversity and function of ecosystems. …”
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Identification of standing dead trees in Robinia pseudoacacia plantations across China’s Loess Plateau using multiple deep learning models by Li Zhang, Xiaodong Gao, Shuyi Zhou, Zhibo Zhang, Tianjie Zhao, Yaohui Cai, Xining Zhao

“…Drought-induced tree mortality has increasingly expanded worldwide under the influence of climate warming, with China’s Loess Plateau (CLP) emerging as a critical hotspot for such impacts. As one of the most active tree-planting regions globally, the CLP primarily aims to achieve soil and water conservation despite facing challenges such as limited rainfall and frequent extreme drought events. …”
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Identification of common and specific fibrosis-related genes in three common chronic kidney diseases by Zhangning Fu, Xiaodong Geng, Chao Liu, Wanjun Shen, Zheyi Dong, Guannan Sun, Guangyan Cai, Xiangmei Chen, Quan Hong

“…Comparative gene expression analysis among these types of CKD may shed light on its pathogenesis. Therefore, we conducted this study aiming at exploring the common and specific fibrosis-related genes involved in different types of CKD.Methods Kidney biopsy specimens from patients with different types of CKD and normal control subjects were analyzed using the NanoString nCounter® Human Fibrosis V2 Panel. …”
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Peri‐ictal yawning: A potential lateralizing sign in temporal lobe epilepsy by Xiaoping Du, Yi Yao, Jiani Chen, Xiaoping Yang, Lei Zhang, Yingying Tang, Xiaoting Hao, Dong Zhou, Shizhong Lian, Fengpeng Wang, Xiaobin Zhang, Meizhen Sun, Junhong Guo

“…However, it can occasionally indicate seizure activity, particularly in TLE. PY happens more often observed in non‐dominant TLE and usually occurs in the later stages of a seizure or just after it. …”
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Qualitative evaluation of the Rehabilitation Exercise and psycholoGical support After COVID-19 InfectioN (REGAIN) randomised controlled trial (RCT): ‘you are not alone’ by Gordon McGregor, Susanne Finnegan, Martin Underwood, Joyce Yeung, James Mason, Peter Heine, Nigel Edwards, Julie Bruce, Kate Seers, Harbinder Kaur Sandhu, Helen Atherton, Sharisse Alleyne, Katie Booth, Ranjit Lall, Vivien P Nichols, Stuart Ennis, Scott McGuire, Rachel Potter, Jessica Smith, Chen Ji, Shilpa Patel, Henry Nwankwo, Kate Evans, Susie Hennings, Alastair Canaway, Grace Lobley, David Mcwilliams, Christina Jones, Emma Padfield, David Montgomery, Beatriz Lara, Becky Haley, Francesca Denton, Bartholomew Sheehan, Mariam Ratna, Elaine Fairbrother, Zoe Noakes, Bogdan Zutic, Lee Austin, Tom Forsyth, Anne Bush, Alan Bush, Patrick Sawdon, Jonathon Prosser, Lee Tompkins, Jonathon Guck, Danielle Hale, Juwairia Said, Georgie Ray, Rowena Williams, Gail Evans, Thillini Hettiarachchi, Abeesh Panicker

“…The themes from group exercise were: (1) monitoring and modification of the online exercise; (2) catering for differing abilities; (3) feeling safe and confident to exercise; (4) progression of fitness; (5) optimal timing in the recovery trajectory; (6) group effect; (7) initial apprehension about exercise group; (8) gauging exercise capabilities; (9) translating exercises into life; and (10) on-demand supplementary videos. …”
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Nutritional and antioxidative characterization, antimicrobial and sensorial stability of flaxseed powder supplemented mutton patties by Raheel Suleman, Hira Choudhary, Muhammad Waseem, Jaza Maqbl Alshammari, Muhammad Muzamil, Huan Liu, Tariq Ismail, Muhammad Ammar Khan, Muhammad Qamar, Crossby Osei Tutu

“…Among extracts, the aqueous flaxseed extracts exhibited the highest total phenolic contents (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) activities i.e., 275 mg GAE/g, 75 % and 57 mg/g, respectively. …”
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