Implementation and validation of a new qPCR assay to detect imported human Plasmodium species by Camille Cordier, Samia Hamane, Théo Ghelfenstein-Ferreira, Sarah Dellière, Élodie Da Silva, Blandine Denis, Sandrine Houzé, Valentin Joste, Alexandre Alanio

“…In this study, we developed a combined molecular tool based on both detection of all human plasmodial species (Plasmodium spp.) and P. falciparum. We have also developed specific qPCRs for each human plasmodial species.…”
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Prevalence and molecular characterization of human bocavirus-1 in children and adults with influenza-like illness from Kunming, Southwest China by Yanhong Sun, Lili Jiang, Yaoyao Chen, Zhaosheng Liu, Meiling Zhang, Xiaonan Zhao, Xiaoyu Han, Lifen Zhang, Xiaoqing Fu, Jienan Zhou

“…Currently, the prevalence of HBoV-1 in the Kunming area is not well characterized, and its molecular features remain inadequately described. This study aims to analyze the prevalence of HBoV-1 among ILI cases in Kunming, encompassing both pediatric and adult patients. …”
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First-year dynamics of the anaerobic microbiome and archaeome in infants’ oral and gastrointestinal systems by Charlotte J. Neumann, Rokhsareh Mohammadzadeh, Pei Yee Woh, Tanja Kobal, Manuela-Raluca Pausan, Tejus Shinde, Victoria Haid, Polona Mertelj, Eva-Christine Weiss, Vassiliki Kolovetsiou-Kreiner, Alexander Mahnert, Christina Kumpitsch, Evelyn Jantscher-Krenn, Christine Moissl-Eichinger

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Sdd3 regulates the biofilm formation of Candida albicans via the Rho1-PKC-MAPK pathway by Li Mei Pang, Guisheng Zeng, Eve Wai Ling Chow, Xiaoli Xu, Ning Li, Yee Jiun Kok, Shu Chen Chong, Xuezhi Bi, Jiaxin Gao, Chaminda Jayampath Seneviratne, Yue Wang

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The two-component system ArlRS is essential for wall teichoic acid glycoswitching in Staphylococcus aureus by Marieke M. Kuijk, Emma Tusveld, Esther Lehmann, Rob van Dalen, Iñigo Lasa, Hanne Ingmer, Yvonne Pannekoek, Nina M. van Sorge

“…Since WTA represents a promising target for future immune-based treatments and vaccines, our findings provide important insight to align strategies targeting S. aureus WTA glycosylation patterns during infection.IMPORTANCEStaphylococcus aureus is a common colonizer but can also cause severe infections in humans. The development of antibiotic resistance complicates the treatment of S. aureus infections, increasing the need for antibiotic alternatives such as vaccines and therapies with bacterial viruses also known as phages. …”
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Characteristic alterations of gut microbiota and serum metabolites in patients with chronic tinnitus: a multi-omics analysis by Jiang Wang, Jia-Hui Xiang, Xu-Yuan Peng, Min Liu, Le-Jia Sun, Min Zhang, Li-Yuan Zhang, Zhi-Bin Chen, Zheng-Quan Tang, Lei Cheng

“…We proposed the innovative concept of the “gut-brain-ear axis,” which underscores the exploration of gut microbiota impact on susceptibility to chronic tinnitus through serum metabolic profile modulation. We also reveal novel biomarkers associated with chronic tinnitus, offering a new conceptual framework for further investigations into the susceptibility of patients, potential treatment targets for tinnitus, and assessing patient prognosis. …”
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Characterization of a novel covS SNP identified in Australian group A Streptococcus isolates derived from the M1UK lineage by Johanna Richter, Amanda J. Cork, Yvette Ong, Nadia Keller, Andrew J. Hayes, Mark A. Schembri, Amy V. Jennison, Mark R. Davies, Kate Schroder, Mark J. Walker, Stephan Brouwer

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The protein composition of human adenovirus replication compartments by Paloma Hidalgo, Amada Torres, Pierre M. Jean Beltran, Gamaliel López-Leal, Luca D. Bertzbach, Thomas Dobner, S. J. Flint, Ileana M. Cristea, Ramón A. González

“…RCs are specialized virus-induced subnuclear microenvironments where not only viral genome replication and expression are orchestrated but also host proteins that restrict viral replication are co-opted and subverted. …”
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Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii group in Taiwan by Tran Lam Tu Quyen, Yu-Chia Hsieh, Shiao-Wen Li, Lii-Tzu Wu, Ya-Zhu Liu, Yi-Jiun Pan

“…All CRAB strains were positive for blaOXA-51-like; 72.6% also carried blaOXA-23-like, 22.8% carried blaOXA-24-like, 3.4% co-carried blaOXA-23-like+blaOXA-24-like, and 1.27% carried blaOXA-51-like alone. …”
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Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling by Walid Mottawea, Basit Yousuf, Salma Sultan, Tamer Ahmed, JuDong Yeo, Nico Hüttmann, Yingxi Li, Nour Elhouda Bouhlel, Hebatoallah Hassan, Xu Zhang, Zoran Minic, Riadh Hammami

“…We hypothesize that MEVs cross host cellular barriers and deliver their cargoes of bioactive compounds to the brain. In this study, we aimed to investigate the cargo capacity of MEVs for bioactive metabolites and their interactions with the host cellular barriers. …”
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Comparative analysis of the human microbiome from four different regions of China and machine learning-based geographical inference by Yinlei Lei, Min Li, Han Zhang, Yu Deng, Xinyu Dong, Pengyu Chen, Ye Li, Suhua Zhang, Chengtao Li, Shouyu Wang, Ruiyang Tao

“…These findings not only contribute to a deeper understanding of the diversity and geographical distribution of human bacteria but also enrich the microbiome data of the Asian population for further studies.…”
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Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections by Yurong Qin, Yiwen Liao, Jingfang Zhou, Weijiang Liu, Huimin Chen, Xiaoli Chen, Weisha Wang, Ni Zhang, Yunhu Zhao, Liang Wang, Bing Gu, Suling Liu

“…In various application scenarios, the VIRTUO system demonstrates a higher positive detection rate, whether in the intensive care unit (ICU) (8.5% vs 6.4%, P = 0.028) or in general wards. Additionally, for different types of bacteria, the TVIRTUO system exhibits superior detection rates for anaerobic bacteria (5.9% vs 3.2%, P < 0.001) and aerobic bacteria (9.1% vs 7.2%, P = 0.043). …”
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Resistome phylodynamics of multidrug-resistant Shigella isolated from diarrheal patients by Asaduzzaman Asad, Md. Abu Jaher Nayeem, Md. Golam Mostafa, Ruma Begum, Shah Nayeem Faruque, Suraia Nusrin, Israt Jahan, Shoma Hayat, Zhahirul Islam

“…The MDR plasmids and resistant GIs were phylogenetically relevant to Europe, USA, or China-derived isolates, indicating carry-over of the emerging ARGs from heavily industrialized countries and MSM-burdened (men who have sex with men) populations. …”
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Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance by Qiwen Tian, Hailv Ye, Xuan Zhou, Junyi Wang, Lifang Zhang, Wenxuan Sun, Chenxin Duan, Minyu Fan, Wei Zhou, Chuyun Bi, Qiong Ye, Aloysius Wong

“…ARGs responsible for erythromycin and streptomycin resistance, which were initially absent in the recipient bacteria, were also detected in the transconjugants. Our data build the foundation for future studies that will be conducted on animal models and in humans and leveraging advanced metagenomics approaches to clarify the long-term health risk of probiotic consumption.…”
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Antibiotic resistance genotype, phenotype, and clinical outcomes in patients with Gram-negative infections at Rabin Medical Center in Israel by Rachelle E. Koch, Jackson Barth, Andrew E. Clark, Dhara Desai, Jiwoong Kim, Christine A. Pybus, Xiaowei Zhan, Leonard Leibovici, Dafna Yahav, David E. Greenberg

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Sharing of cmeRABC alleles between C. coli and C. jejuni associated with extensive drug resistance in Campylobacter isolates from infants and poultry in the Peruvian Amazon by Kerry K. Cooper, Evangelos Mourkas, Francesca Schiaffino, Craig T. Parker, Tackeshy N. Pinedo Vasquez, Paul F. Garcia Bardales, Pablo Peñataro Yori, Maribel Paredes Olortegui, Katia Manzanares Villanueva, Lucero Romaina Cachique, Hermann Silva Delgado, Matthew D. Hitchings, Steven Huynh, Samuel K. Sheppard, Ben Pascoe, Margaret N. Kosek

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Performance of a hybrid capture-based target enrichment next-generation sequencing for the identification of respiratory pathogens and resistance-associated genes in patients with... by Wei-Yu Hsu, Ting-Wei Kao, Hsin-Ching Cho, Sheng-Yuan Ruan, Tai-Fen Lee, Yu-Tsung Huang, Jung-Yien Chien

“…Unlike conventional phenotypic approach, novel measures using molecular interrogation appear promising. This study aimed to elucidate the efficacy of a hybrid capture-based target enrichment next-generation sequencing technique (Respiratory Pathogen ID/AMR Enrichment Panel, RPIP) as exemplified in a cohort with severe pneumonia. …”
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Modification and analysis of context-specific genome-scale metabolic models: methane-utilizing microbial chassis as a case study by M. A. Kulyashov, R. Hamilton, Y. Afshin, S. K. Kolmykov, T. S. Sokolova, T. M. Khlebodarova, M. G. Kalyuzhnaya, I. R. Akberdin

“…ABSTRACT Context-specific genome-scale model (CS-GSM) reconstruction is becoming an efficient strategy for integrating and cross-comparing experimental multi-scale data to explore the relationship between cellular genotypes, facilitating fundamental or applied research discoveries. …”
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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…This finding defines some of the pivotal mechanisms that B. burgdorferi evolved to survive oxidative conditions experienced with tick and mammal immune responses. These observations also provide an opportunity for innovative vaccine development strategies employing ionizing radiation to disrupt the B. burgdorferi genome, while maintaining antigenic potency. …”
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Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulence by Dana S. Franklin, Yi-Wei Chen, Yimin Chen, Manuel Wittchen, Angela Agnew, Alexis Luu, Julian P. Whitelegge, Z. Hong Zhou, Andreas Tauch, Asis Das, Hung Ton-That

“…BMCs are also assembled in fusobacteria cultured with placental cells or the culture media, a process that is dependent on the BMC shell proteins. …”
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