Performance of deep learning models in predicting the nugent score to diagnose bacterial vaginosis by Naoki Watanabe, Tomohisa Watari, Kenji Akamatsu, Isao Miyatsuka, Yoshihito Otsuka

“…However, its accuracy depends on the skills of laboratory technicians. This study aimed to evaluate the performance of deep learning models in predicting the Nugent score to improve diagnostic consistency and accuracy. …”
Get full text

Genetic and biochemical characterization of a radical SAM enzyme required for post-translational glutamine methylation of methyl-coenzyme M reductase by Roy J. Rodriguez Carrero, Cody T. Lloyd, Janhavi Borkar, Shounak Nath, Liviu M. Mirica, Satish Nair, Squire J. Booker, William Metcalf

Get full text

Distinct spatiotemporal patterns between fungal alpha and beta diversity of soil–plant continuum in rubber tree by Yaqing Wei, Zhixiang Wu, Guoyu Lan

Get full text

Cohort profile: the BangladEsh Longitudinal Investigation of Emerging Vascular and nonvascular Events (BELIEVE) cohort study by Anisur Rahman, Stephen Sutton, Mahbubur Rahman, Jason Crawte, Tahmeed Ahmed, Md Saimul Islam, Aliya Naheed, Adam S Butterworth, Emanuele Di Angelantonio, Matthew Walker, John Danesh, Angela Wood, Sarah Hawkes, Sadika Akhter, Catherine Perry, Aytalina Azarova, Ruchira T Naved, Meerjady Sabrina Flora, Nusrat Khan, Rajiv Chowdhury, Sohel Reza Choudhury, Nusrat Jahan, Stephen Kaptoge, Md Zahidul Islam, Kaniz Fatema, Kim Robin van Daalen, Shammi Luhar, Roderic L Jones, Abbas Bhuiya, Md Khalequzzaman, Syed Shariful Islam, Henry Taylor, Shamima Akter, Simon Griffin, Khairul Islam, Salma Akter, Ruchira Tabassum Naved, Abdul Malik, Rubhana Raqib, Animesh Talukder, Richard Houghton, Tahmina Shirin, Lawrence King, Kamrun Nahar, Samantha Farrow, Giulia Loffreda, Samsad Rabbani Khan, Valerie Rhenius, Nahida Akter, Md Mostafa Monower, Sabina Akter, Mantaka Rahman, Lisa Pennells, Maria L C Iurilli, Md Taslim Uddin Miah, Khan Mohammad Thouhidur Rahman, Sharraf Samin, Kazi Nazmus Saqeeb, Ishrat Tasmin, Eleanor Farrow, Ank Michielsen, Sarah Spackman, Charlotte van Coeverden, James Ajioka, Khondker Abdul Abdul Awal, Evangelia Chatzidiakou, Jörg Feldmann, Richard Fenner, Tuhin Haque, Sirajul Islam, Kamrul Hasan Khan, Fazila-Tun-Nesa Malik, Olalekan Popoola, Nicholas Mascie Taylor, Md Alfazal Khan, Israt Akter, Laboni Akter, Shoriful Islam, Mohammad Kamruzzaman, Monalisa Moni, A H M Rezwan, K M Thouhidur Rahman, Monjeline Sultana, Jesmin Akhter, Ayasha Akter, Bakul Akter, Jharna Akter, Jesmin Akter, Juba Akter, Khadiza Akter, Khadija Akter, Lipi Akter, Maksuda Akter, Mousumi Akter, Mst Lovely Akter, Nasima Akter, Samima Akter, Sema Akter, Shahida Akter, Shahnaj Akter, Taslima Akter, A T M Zorjis Alam, Mahmuda Atique, Lutfa Begum, Mst Nazma Begum, Farjana Choudhury, Mitali Paul Chowdhury, Robin Reza Chowdhury, Mukul Rani Debnath, Nahid Ferdash, Naima Ferdous, Md Rakib Al Hasan, Khandaker Hashanuzzaman, Shamima Haq, Md Riazul Haque, Alamgir Hossain, Md Ibrahim, Shahi Israt Jahan, Israt Jahan Jarin, Zohora Pradhan Jonaki, Asik Kabir, Tonema Kader, Md Mostafa Kamal, Sayed Kamruzzaman, Sadik Fatima Kanon, Nazmul Karim, Shamsul Karim, Tanuja Khanom, Shamim Ara Khatun, Badrun Nahar Lorin, Mst Sirajum Manira, Farhana Jahan Mary, Kazi Dilruba Mita, Lipi Mitra, Basudeb Mollik, Sudipta Nargis, Nusrat Alam Nawmee, Esrat Zahan Nesa, Mahmuda Akter Nipa, Mehenaz Parvin, Sanjida Parvin, Shahnaj Parvin, Suraiya Parvin, Nayan Rabidash, Rulia Rahman, Madhabi Rani, Shahjalal Sarker, Smriti Sarker, Razia Sultana Shathi, Fatema Shelly, Ireen Sultana, Rovaiya Sultana, Israt Jahan Sumi, Sharmin Tamanna, Khadija Akter Topy, Umme Habiba Urmee, Suraya Yesmin, Julianne Halley, Charlotte van Coeverden, Mahmuda Akther Akhi, Asia Akter, Labony Akter, Ms Mili Akter, Mst Shamima Akter, Setu Akter, Tahmina Akter, Md Sabdar Ali, Mst Jesmin Ara, Edyta Bujnik, Apu Chandra Das, Many Das, Nurjahan Fatema, Md Riyad Hasan, Soniya Jannat, Mst Amena Khatun, Most Nurnahar Khatun, William Mossman, Robyn Murdoch, Zannaton Naeem, Evrikleia Ntasi, Silvia Alonso Rodriguez, Tripty Roy, Most Abida Sultana, Laryssa Amado, Tanya Braune, Eilidh Cowan, Steve Ellis, Ellie Farrow, Hannah Lombardi, Niko Ovenden, Tamara Sabri, Md Khalid Sultan, Jim Ajioka, Khondker Abdul Awal, Arul Baradi, Emanuele Di Angelantonio, Camilla Faidutti, Joerg Feldmann, Louise Hair, Sharifuddin Hasnat, Shahid Akhter Hossain, Md Mominul Islam, Shafiul Islam, Joe Lavallée, Fazila Tun-Nesa Malik, Nick Mascie-Taylor, Md Sirajul Islam, Laurie Savage, Sara Shazad, Lalitha Sundaram, Aloka Tulukdar, Kim Van Daalen, Tanvir Chowdhury, Nurul Huda, Samia Naz Isha, Riaz Hossain Khan, Aliva Salmeen, Zeeba Zahra Sultana

“…Overall, the mean (SD) body mass index (BMI) was 25 (5) kg/m2, with 10 442 (16%) participants aged 20–79, classified as obese (ie, BMI≥30 kg/m2). Mean BMI was also higher in urban than slum and rural areas.Future plans The collection of information during the baseline visit was completed in 2020. …”
Get full text

A novel multimodality anthropomorphic phantom enhances compliance with quality assurance guidelines for magnetic resonance imaging in radiotherapy by Meshal Alzahrani, David A Broadbent, Irvin Teh, Bashar Al-Qaisieh, Emily Johnstone, Richard Speight

“…Background and purpose: The use of magnetic resonance imaging (MRI) for radiotherapy (RT) simulation has grown, prompting quality assurance (QA) guidelines by the Institute of Physics and Engineering in Medicine (IPEM) and the American Association of Physicists in Medicine (AAPM). This study compares a novel multimodality anthropomorphic phantom to an American College of Radiology (ACR) phantom for a subset of these MRI-specific QA tests in RT. …”
Get full text

Associated Factors of the Need for Mechanical Ventilation Following Traumatic Injuries; a Registry-Based Study on 2,708 Cases in Iran by Zahra Ramezani, Vali Baigi, Mohammadreza Zafarghandi, Vafa Rahimi-Movaghar, Reza Farahmand-Rad, Akram Zolfaghari Sadrabad, Seyed-Mohammad Piri, Mahgol Sadat Hassan Zadeh Tabatabaei, Khatereh Naghdi, Payman Salamati

“… Introduction: Traumatic injuries can affect respiration both directly and indirectly. This study aimed to evaluate the predictive factors of need for mechanical ventilation (MV) following traumatic injuries. …”
Get full text

A temporary cholesterol-rich diet and bacterial extracellular matrix factors favor Salmonella spp. biofilm formation in the cecum by Alonso Cruz-Cruz, Megan E. Schreeg, John S. Gunn

“…It highlights not only a cholesterol-rich diet as an important etiological factor for Salmonella colonization but also identifies the cecum as a crucial tissue promoting fecal shedding. …”
Get full text

Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa by Dorota Kuc-Ciepluch, Karol Ciepluch, Daria Augustyniak, Grzegorz Guła, Barbara Maciejewska, Artur Kowalik, Ewelina Jop, Zuzanna Drulis-Kawa, Michał Arabski

“…However, the pyroptosis stimulation by LPS in the antibacterial assay was not effective enough for bacterial OM permeabilization and enhancement of endolysin activity. We assume that leveraging pyroptosis induction in monocytic cells to augment the bactericidal activity of endolysins may be limited.IMPORTANCERecombinant GSDMDNterm protein was able to efficiently permeabilize P. aeruginosa outer membranes and increase endolysin activity against bacteria, producing either long LPS O-chains or lack them entirely. …”
Get full text

Identification and characterization of the functional tetrameric UDP-glucose pyrophosphorylase from Klebsiella pneumoniae by Isabel Ramón Roth, Pavel Kats, Timm Fiebig, Françoise Routier, Roman Fedorov, Larissa Dirr, Jana I. Führing

Get full text

Spectrum of bacterial keratitis at a tertiary eye center in Indonesia by Rifna Lutfiamida, Rien Widyasari, Kara Citra Kalandra

“…Understanding the spectrum of bacterial pathogens causing keratitis is crucial for guiding appropriate treatment strategies and improving patient outcomes. This study aims to investigate the prevalence, antibiotic susceptibility patterns, and risk factors associated with bacterial keratitis cases, providing valuable insights for clinicians to optimize management protocols and enhance the overall ocular health landscape in Indonesia or in other countries that have similar geographical conditions.…”
Get full text

Clinical laboratory evaluation of the Hologic Panther Aptima BV and CV/TV assays for the diagnosis of vaginitis in Dunedin, Aotearoa New Zealand by Juliet Elvy, Katelyn Carter, Jenna Paterson, Megan Smith, Gayleen Parslow, James E. Ussher

Get full text

Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The bioluminescent strains demonstrated stable plasmid retention and high bioluminescence levels. They also showed growth comparable to their parent strains, even in the absence of antibiotics. …”
Get full text

Magnetic nanoparticles of Nd2Fe14B prepared by ethanol-assisted wet ball milling technique by Younes Mehrifar, Hamed Moqtaderi, Seyedeh Mehri Hamidi, Farideh Golbabaei, Mahdi Hasanzadeh, Somayeh Farhang Dehghan

“…The demand for the production of these nanoparticles is significantly high due to their exceptional properties. The aim of the present study is to synthesize magnetic nanoparticles of Nd2Fe14B using ethanol in the wet ball milling technique (WBMT). …”
Get full text

Genome-resolved year-round dynamics reveal a broad range of giant virus microdiversity by Yue Fang, Lingjie Meng, Jun Xia, Yasuhiro Gotoh, Tetsuya Hayashi, Keizo Nagasaki, Hisashi Endo, Yusuke Okazaki, Hiroyuki Ogata

“…We argue that greater microdiversity of viruses likely enhances adaptability and thus survival under the virus–host arms race during prolonged interactions with their hosts.IMPORTANCERecent genome-resolved metagenomic surveys have uncovered the vast genomic diversity of giant viruses, which play significant roles in aquatic ecosystems by acting as bloom terminators and influencing biogeochemical cycles. …”
Get full text

Stage-specific function of sphingolipid synthases in African trypanosomes by Norton Heise, Carolina M. Koeller, Mohamed Sharif, James D. Bangs

“…ABSTRACT The protozoan parasite Trypanosoma brucei is the only known eukaryote capable of synthesizing the three main phosphosphingolipids: sphingomyelin (SM), inositol phosphorylceramide (IPC), and ethanolamine phosphorylceramide (EPC). …”
Get full text

Development of cohort definitions and algorithms to identify patients with Lennox-Gastaut syndrome or Dravet syndrome from real-world administrative healthcare databases by Drishti Shah, Victoria Divino, Justin Chen, Arturo Benitez, Jeannine Roth, J. Scott Andrews

“…Country-specific research explored the diagnosis codes and antiseizure medications (ASMs) with available indications for LGS or DS, with the findings used to adapt the cohort definitions and algorithms to country-specific settings. …”
Get full text

New insights in the low-temperature-dependent formation of amorphous titania-coated magnetic polydopamine nanocomposites for the adsorption of methylene blue by Jiangqun Zhang, Xingyu Long, Meng Hu, Wanli Wei, Jiangning Gong, Chun Yang, Pinhua Xia, Anping Wang

“…To analyze the magnetic properties of the core, the amorphous structure of the shell, and the surface properties (size and morphology, elemental composition, functional groups, crystal structure, magnetic properties, etc.) of FPaT, various analytical techniques including scanning electronic microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM) were utilized to characterize it. Based on the unique physicochemical properties of its surface, the FPaT was applied to highly adsorb methylene blue (MB) from simulated wastewater. …”
Get full text

Efficacy of ceftazidime-avibactam with or without polymyxin for carbapenem-resistant Klebsiella pneumoniae infections after initial treatment with polymyxin by Jingli Lu, Yani Ma, Zhe Cao, Baoling Zhu, Luna Fan, Haiyang Meng

“…Also, 91 (85.8%) patients received CAZ-AVI combination therapy, and 34 (32.1%) patients received CAZ-AVI in combination with polymyxin B. …”
Get full text

A novel framework unveiling the importance of heterogeneous selection and drift on the community structure of symbiotic microbial indicator taxa across altitudinal gradients in amp... by Jin Zhou, Zhidong Liu, Sishuo Wang, Jing Li, Lin Zhang, Ziyan Liao

“…Notably, the relative contribution of heterogeneous selection was significantly positively correlated with the relative abundance and niche breadth of taxa, and negatively correlated with drift. We also detected that community assembly processes remarkably regulate the structure of symbiotic microbial communities and their correlation with environmental variables. …”
Get full text

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…This conditional knockdown method will be useful for studying the function of genes in Chlamydia. It also has the potential to be applied to other obligate intracellular bacteria, including Rickettsia and Coxiella.…”
Get full text