Identification and characterization of the functional tetrameric UDP-glucose pyrophosphorylase from Klebsiella pneumoniae by Isabel Ramón Roth, Pavel Kats, Timm Fiebig, Françoise Routier, Roman Fedorov, Larissa Dirr, Jana I. Führing

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Construction and characterization of bioluminescent Salmonella Reading outbreak and non-outbreak strains by Abubakar Shitu Isah, Reshma Ramachandran, Anuraj Theradiyil Sukumaran, Aaron S. Kiess, Claudia D. Castañeda, Timothy Boltz, Kenneth S. Macklin, Hossam Abdelhamed, Li Zhang

“…The bioluminescent strains demonstrated stable plasmid retention and high bioluminescence levels. They also showed growth comparable to their parent strains, even in the absence of antibiotics. …”
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Magnetic nanoparticles of Nd2Fe14B prepared by ethanol-assisted wet ball milling technique by Younes Mehrifar, Hamed Moqtaderi, Seyedeh Mehri Hamidi, Farideh Golbabaei, Mahdi Hasanzadeh, Somayeh Farhang Dehghan

“…The demand for the production of these nanoparticles is significantly high due to their exceptional properties. The aim of the present study is to synthesize magnetic nanoparticles of Nd2Fe14B using ethanol in the wet ball milling technique (WBMT). …”
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Genome-resolved year-round dynamics reveal a broad range of giant virus microdiversity by Yue Fang, Lingjie Meng, Jun Xia, Yasuhiro Gotoh, Tetsuya Hayashi, Keizo Nagasaki, Hisashi Endo, Yusuke Okazaki, Hiroyuki Ogata

“…We argue that greater microdiversity of viruses likely enhances adaptability and thus survival under the virus–host arms race during prolonged interactions with their hosts.IMPORTANCERecent genome-resolved metagenomic surveys have uncovered the vast genomic diversity of giant viruses, which play significant roles in aquatic ecosystems by acting as bloom terminators and influencing biogeochemical cycles. …”
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Stage-specific function of sphingolipid synthases in African trypanosomes by Norton Heise, Carolina M. Koeller, Mohamed Sharif, James D. Bangs

“…ABSTRACT The protozoan parasite Trypanosoma brucei is the only known eukaryote capable of synthesizing the three main phosphosphingolipids: sphingomyelin (SM), inositol phosphorylceramide (IPC), and ethanolamine phosphorylceramide (EPC). …”
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Development of cohort definitions and algorithms to identify patients with Lennox-Gastaut syndrome or Dravet syndrome from real-world administrative healthcare databases by Drishti Shah, Victoria Divino, Justin Chen, Arturo Benitez, Jeannine Roth, J. Scott Andrews

“…Country-specific research explored the diagnosis codes and antiseizure medications (ASMs) with available indications for LGS or DS, with the findings used to adapt the cohort definitions and algorithms to country-specific settings. …”
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A novel framework unveiling the importance of heterogeneous selection and drift on the community structure of symbiotic microbial indicator taxa across altitudinal gradients in amp... by Jin Zhou, Zhidong Liu, Sishuo Wang, Jing Li, Lin Zhang, Ziyan Liao

“…Notably, the relative contribution of heterogeneous selection was significantly positively correlated with the relative abundance and niche breadth of taxa, and negatively correlated with drift. We also detected that community assembly processes remarkably regulate the structure of symbiotic microbial communities and their correlation with environmental variables. …”
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Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…This conditional knockdown method will be useful for studying the function of genes in Chlamydia. It also has the potential to be applied to other obligate intracellular bacteria, including Rickettsia and Coxiella.…”
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Optimizing in vitro spherulation cues in the fungal pathogen Coccidioides by Christina M. Homer, Elena Ochoa, Mark Voorhies, Anita Sil

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Identification and characterization of Faecalibacterium prophages rich in diversity-generating retroelements by Anastasia Gulyaeva, Lei Liu, Sanzhima Garmaeva, Marloes Kruk, Rinse K. Weersma, Hermie J. M. Harmsen, Alexandra Zhernakova

“…DGRs of two phages were active at the time of the induction experiments, as evidenced by nucleotide variation in sequencing reads. We also predicted that the host range of two active phages may include multiple bacterial species. …”
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Multimedia: multimodal mediation analysis of microbiome data by Hanying Jiang, Xinran Miao, Margaret W. Thairu, Mara Beebe, Dan W. Grupe, Richard J. Davidson, Jo Handelsman, Kris Sankaran

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Perforin-2 is dispensable for host defense against Aspergillus fumigatus and Candida albicans by Mariano A. Aufiero, Li-Yin Hung, De'Broski R. Herbert, Tobias M. Hohl

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Dextran sodium sulfate-induced colitis alters the proportion and composition of replicating gut bacteria by Eve T. Beauchemin, Claire Hunter, Corinne F. Maurice

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Synthesis and Characterization of Copper Ferrite Nanoparticles for Efficient Photocatalytic Degradation of Organic Dyes by Julia Mazurenko, A. K. Sijo, Larysa Kaykan, Volodymyr Kotsyubynsky, Łukasz Gondek, Antoni Zywczak, Mateusz Marzec, Oleksii Vyshnevskyi

“…The effect of hydrogen peroxide concentration on dye degradation efficiency was also evaluated.…”
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An adenoviral vector encoding an inflammation-inducible antagonist, HMGB1 Box A, as a novel therapeutic approach to inflammatory diseases by Kari Ann Shirey, John Joseph, Lynda Coughlan, Haye Nijhuis, Alan W. Varley, Jorge C. G. Blanco, Stefanie N. Vogel

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mtlA, a novel typing gene offering enhanced resolution for pandemic Vibrio species by Lei Zhou, Danlei Liu, Zilong Zhang, Yongqiang Zhu, Huajun Zheng

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Discovery of reproductive tissue-associated bacteria and the modes of microbiota acquisition in male honey bees (drones) by Alexis Burks, Patrick Gallagher, Kasie Raymann

“…ABSTRACT Honey bees are the third most economically important agricultural animal in the world due to their role as pollinators. …”
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Hindguts of Kyphosus sydneyanus harbor phylogenetically and genomically distinct Alistipes capable of degrading algal polysaccharides and diazotrophy by Cesar T. Facimoto, Kendall D. Clements, W. Lindsey White, Kim M. Handley

“…However, Alistipes are also prevalent in the hindgut of the marine herbivorous fish Kyphosus sydneyanus (Silver Drummer), and analysis of their carbohydrate-active enzyme (CAZyme) encoding gene repertoires suggests Alistipes degrade macroalgal biomass to support fish nutrition. …”
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Skin-associated Corynebacterium amycolatum shares cobamides by M. H. Swaney, N. Henriquez, T. Campbell, J. Handelsman, L. R. Kalan

“…This study provides a unique model to study metabolite sharing by skin microorganisms, which will be critical for understanding the fundamental interactions that occur within the skin microbiome and for developing therapeutic approaches aiming to engineer and manipulate the skin microbiota.…”
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Short-term evolution and dispersal patterns of fluconazole-resistance in Candida auris clade III by Irving Cancino-Muñoz, Juan Vicente Mulet-Bayona, Carme Salvador-García, Nuria Tormo-Palop, Remedios Guna, Concepción Gimeno-Cardona, Fernando González-Candelas

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