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  1. 11761
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  3. 11763

    A protocol for loading Calcein-AM into extracellular vesicles from mammalian cells for clear visualization with a fluorescence microscope coupled to a deconvolution system. by María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero

    Published 2025-01-01
    “…In this study, we adapted a protocol originally designed for EVs analysis by flow cytometry using Calcein-AM, and convert it into a useful and effective tool for visualizing EVs by epifluorescence microscopy coupled with a deconvolution system. …”
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  4. 11764
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  8. 11768

    Esketamine at a Clinical Dose Attenuates Cerebral Ischemia/Reperfusion Injury by Inhibiting AKT Signaling Pathway to Facilitate Microglia M2 Polarization and Autophagy by Gao Y, Li L, Zhao F, Cheng Y, Jin M, Xue FS

    Published 2025-01-01
    “…Ying Gao,1 Lu Li,1 Fang Zhao,1 Yi Cheng,1 Mu Jin,2 Fu-Shan Xue1,3 1Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, People’s Republic of China; 3Department of Anesthesiology, Shengli Clinical Medical College of Fujian Medical University, Fuzhou University Affiliated Provincial Hospital, Fujian Provincial Hospital, Fuzhou, People’s Republic of ChinaCorrespondence: Mu Jin, Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, No. 2 An-Zhen Road, Chao-Yang District, Beijing, 100029, People’s Republic of China, Tel +86-13366600987, Email jinmu0119@hotmail.com Fu-Shan Xue, Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, No. 95 Yong-An Road, Xi-Cheng District, Beijing, 100050, People’s Republic of China, Tel +86-13911177655, Fax +86-591-88217841, Email xuefushan@aliyun.com; xuefushan@mail.ccmu.edu.cnPurpose: This study aimed to assess the protective effect of a clinical dose esketamine on cerebral ischemia/reperfusion (I/R) injury and to reveal the potential mechanisms associated with microglial polarization and autophagy.Methods: Experimental cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in adult rats and simulated by oxygen-glucose deprivation (OGD) in BV-2 microglial cells. …”
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  9. 11769
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  13. 11773

    The Influence of the Preservation Method and Gamma Irradiation Sterilization on TGF-β and bFGF Levels in Freeze-Dried Amnion Membrane (FD-AM) and Amnion Sponge by Heri Suroto, Deny M. Aryawan, Camilla A. Prakoeswa

    Published 2021-01-01
    “…There were significant decreases in amnion sponge compared to the FD-AM, both in transforming growth factor beta (TGF-β) and basic fibroblast growth factor (bFGF) levels and in the preirradiated and 25kGy postirradiated preparations (p0.05). …”
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  14. 11774

    Rosiglitazone Restores Endothelial Dysfunction in a Rat Model of Metabolic Syndrome through PPARγ- and PPARδ-Dependent Phosphorylation of Akt and eNOS by Zhigang Zhao, Zhidan Luo, Peijian Wang, Jing Sun, Hao Yu, Tingbing Cao, Yinxing Ni, Jing Chen, Zhencheng Yan, Daoyan Liu, Zhiming Zhu

    Published 2011-01-01
    “…These effects were also abolished by inhibition of PPARγ, PPARδ, or PI3K. In conclusion, rosiglitazone improved endothelial function through both PPARγ- and PPARδ-mediated phosphorylation of Akt and eNOS, which might help to reconsider the complex effects and clinical applications of rosiglitazone.…”
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    Primeras pruebas de adaptación al cultivo extensivo del robalo (Centropomus nigrescens) en estanques en la costa de Michoacán, México by Sergio Escárcega-Rodríguez

    Published 2018-01-01
    “…Considerando los atributos acuícolas de los robalos (Centropomidae) y su más alto precio en el mercado nacional, se analiza la aclimatación en estanques de Centropomus nigrescens, la especie de mayor talla entre los robalos americanos, en agua dulce y agua salobre, como una primera contribución en México para aportar las bases de su cultivo. …”
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  20. 11780