外源性人β防御素-2调节子宫内膜异位症细胞增殖/凋亡及炎性因子的表达 by 徐丽南, 王子莲, 陈淑琴

“…【方法】 组织块消化法原代培养EMs细胞,Western Blot法检测EMs细胞hβD-2蛋白、TNF-α及IL-1β的蛋白表达;Western Blot法检测加入重组人hβD-2蛋白(40μg/mL)前后EMs细胞内TNF-α,IL-1β蛋白表达变化;按不同梯度浓度(80、40、20、10 μg/mL)加入人β防御素-2蛋白(hβD-2)后,ELISA法检测EMs细胞培养液上清中TNF-α,IL-1β细胞因子的浓度;加入高剂量hβD-2蛋白(120 μg/mL)并培养EMs细胞24H设为实验组,对照组为完全培养基培养的EMs细胞,MTT法检测实验组及对照组EMs细胞增殖情况;PI法检测实验组及对照组EMs细胞凋亡情况.【结果】 原代培养的EMs细胞可表达hβD-2蛋白、TNF-α、IL-1β蛋白;加入外源性重组人hβD-2蛋白后,EMs细胞表达TNF-α、IL-1β蛋白下调;EMs细胞培养液上清中可检测出TNF-α,IL-1β的表达;在加入hβD-2后,EMs细胞培养液上清内TNF-α、IL-1β表达下降,且随着加入hβD-2浓度增高其下降趋势增大,差异有统计学意义(80>40>20 > 10 μg/mL,P < 0.05);在EMs细胞培养基中加入高浓度外源性人β防御素2蛋白孵育后(120 μg/mL),与对照组相比,EMs细胞的增殖受到明显抑制,且凋亡明显增加(P < 0.05).…”
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Design, synthesis, structural characterization, in vitro anticancer activity, and in silico studies of some new hydroxylated and fluorinated-substituted hydrazone derivatives by Reşit Çakmak, Eyüp Başaran, Ömer Erdoğan, Suraj N. Mali, Semiha Köprü, Haya Yasin, Shailesh S. Gurav, Giray Topal

“…In this study, novel hydroxylated and fluorinated-substituted hydrazone derivatives bearing an aromatic nitro moiety (2a-d and 3a-d) were designed as potential anticancer drug candidates, synthesized for the first time, and evaluated for their anticancer activity against chondrosarcoma (SW1353), a common primary malignant cartilage-forming tumor, neuroblastoma (SH-SY5Y), a type of brain cancer, and healthy (L929) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method for 24 h. The chemical structures of the target molecules were confirmed by FT-IR, 1D NMR (1H- and 13C- NMR, and APT), 2D NMR (COSY, HETCOR, and HMBC), and elemental analysis. …”
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Kangtaizhi Granule Alleviated Nonalcoholic Fatty Liver Disease in High-Fat Diet-Fed Rats and HepG2 Cells via AMPK/mTOR Signaling Pathway by Jiaxin Zhang, Haixia Du, Menglan Shen, Zhengqi Zhao, Xinmiao Ye

“…In an in vitro study, HepG2 cells were incubated with 1 mM FFA to induce lipid accumulation with or without KTZG treatment. MTT assay, intracellular TG level, oil red O staining, and glucose uptake in cells were detected. …”
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Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and q... by Asghar Narmani, Saeid Ganji, Maryam Amirishoar, Fatemeh Hajikarimi, Aynaz Mazandarani, Mahya Karimi, Ali Mohammadinejad, Azin Azadpour, Roghayyeh Jahedi, Elham Assadpour, Seid Mahdi Jafari

“…Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC50 = 100 nm). …”
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One-pot synthesis and characterization of a BSA-Cu2S/CuS quantum dots drug delivery system based on biuret’s reaction by Porfirio Estrada-Rojas, Juan Francisco Hernández-Paz, Claudia Alejandra Rodríguez-González, Mónica Elvira Mendoza-Duarte, Santos Adriana Martel-Estrada, Laura Elizabeth Valencia-Gómez, Héctor Camacho-Montes, Imelda Olivas-Armendáriz

“…Transmission electron microscopy (TEM) confirmed crystalline structure of the CuS and Cu _2 S quantum dots, with particle size measurements aligning with expectations. Finally, MTT assays confirmed that the synthesized quantum dots are non-toxic, further supporting their potential for biomedical applications.…”
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半边旗二萜类化合物5F对人翼状胬肉成纤维细胞结缔组织生长因子表达和胶原合成的影响 by 林意玲, 吴平, 廖海兰, 张培华, 何惠娟, 江黎明, 梁念慈

“…[方法]利用手术切除的人翼状胬肉组织进行原代细胞培养,即得到HPF.将不同质量不同浓度（0、8、32、128）μg/mL的5F作用于HPF 24 h,通过噻唑蓝比色法（MTT）检测5F对细胞增殖的作用;用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹（Western blot）检测5F对细胞中CTGFmRNA及蛋白表达水平的影响;用化学比色法检测5F对细胞胶原蛋白含量的影响.…”
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3D-Printed PCL-Based Scaffolds with High Nanosized Synthetic Smectic Clay Content: Fabrication, Mechanical Properties, and Biological Evaluation for Bone Tissue Engineering by Furtado ASA, Cunha MHS, Sousa LMR, Brito GC, Verde TFCL, Filgueiras LA, Sobral-Silva LA, Santana MV, Sousa GF, Santos FEP, Mendes AN, Figueredo-Silva J, Maia Filho ALM, Marciano FR, Vasconcellos LMR, Lobo AO

“…Biological performance was assessed through MTT assays (cell viability), alkaline phosphatase activity, histological analysis, and Raman spectroscopy, highlighting the scaffolds’ biocompatibility and potential applications in regenerative medicine.Results: The developed inks demonstrated excellent injectability, and the 3D-printed PCL/LAP scaffolds exhibited a microporous and rough structure, good structural fidelity, low degradability, thermal stability, and sufficient mechanical strength across all formulations. …”
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RETRACTED ARTICLE: Research on the efficacy of Celastrus Orbiculatus in suppressing TGF-β1-induced epithelial-mesenchymal transition by inhibiting HSP27 and TNF-α-induced NF-κB/Sna... by Yaodong Zhu, Yanqing Liu, Yayun Qian, Xiaojun Dai, Ling Yang, Jue Chen, Shiyu Guo, Tadashi Hisamitsu

“…In vitro, a well-established experimental EMT model involving transforming growth factor β1 (TGF-β1) was applied. …”
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Defisiensi Protein S pada Trombosis Vena Dalam by Rinni Andriani, Irza Wahid

“…Trombosis Vena Dalam ditegakkan berdasarkan keluhan dan data klinis yaitu Wells score lebih dari 3 (risiko tinggi), PT 14 detik, APTT 41,7 detik, nilai D Dimer 3152,5 ng/mL dan hasil echovasculer dengan ditemukannya tanda-tanda TVD pada vena poplitea dekstra. …”
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High-Power MHz to 100s of GHz Circuits and Systems for Nuclear Fusion Reactors With Examples From SST-1 and ITER by Kamal K. Samanta

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A rugalmasítás határán: bérmunkaviszonyok a kertészeti ágazatban by András Vigvári, Krisztina Németh

“…Emellett megvilágítjuk a munkavállalói perspektívát is: bemutatjuk a mezőgazdasági bérmunkások társadalmi helyzetét, sérülékenységét, valamint néhány olyan jellemzőt, amit vonzónak, vagy problémának tekintenek a kertészeti kis- és nagyüzemek munkaszervezetében. …”
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Filozófiai tudás vs. filozofálás kontra filozófiai elmélet vs. gyakorlat. A praxis-vita tanulságai by László Nemes

“… Bizonyos értelemben csapdát állítottam a hozzászólóknak, hiszen azáltal, hogy a szerzők válaszoltak a felvetett pontokra, maguk is részévé váltak annak a közös filozofálási folyamatnak, gyakorlatnak, amit bemutatni igyekeztem. A filozófia inkább egy gondolati aktus és közösségi eszmecsere, semmint birtokolt tudás, ennek performatív megnyilvánulásaként értelmezhető e cikkek sorozata is. …”
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Predictive nomogram for postoperative lower-limb deep vein thrombosis in patients undergoing endoscopic endonasal surgery during hospitalization: a retrospective cohort study by Hai Peng, Ruofei Yuan, Zhe Zhang, Ying Wang, Xingchao Wang, Bo Wang, Peng Li

“…Significant predictors included older age, intraoperative bleeding, female gender, prolonged surgery duration, elevated postoperative APTT and D-dimer levels, and disturbance of consciousness. …”
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SAFETY OF BRONCHOALVEOLAR LAVAGE IN HEMATOLOGICAL PATIENTS WITH THROMBOCYTOPENIA – A RETROSPECTIVE COHORT STUDY by Ivan GUR, Roei Tounek, Yaniv Dotan, Elite Vainer Evgrafov, Stav Rakedzon, Eyal Fuchs

“…No association was found between any of the complications recorded and preprocedural platelets, age, aPTT, PT, hematological status, or platelet transfusion.  …”
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Gambaran Perdarahan Intrakranial pada Perdarahan akibat Defisiensi Vitamin K (PDVK) di RSUP Dr. M. Djamil by Rizka Hanifa, Iskandar Syarif, Yusri Dianne Jurnalis

“…Hasil pemeriksaan laboratorium terbanyak dengan nilai hemoglobin 5-10 g/dl (62,5%), nilai trombosit >200.000 (84,37%), nilai PT memanjang (65,62%), dan APTT memanjang (75%). Perdarahan multipel merupakan jenis perdarahan terbanyak. …”
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Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats by Sudha Govindan, Angu Jayabal, Jayasakthi Shanmugam, Prasanna Ramani

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Anticoagulation Management During ECMO: Narrative Review by Jaromir Vajter, Oksana Volod

“…Unfractionated heparin (UFH) remains the most commonly used anticoagulant, monitored via activated partial thromboplastin time (aPTT) or activated clotting time (ACT). Increasing attention is given to alternative tools like anti-Xa and viscoelastic assays (VEA), which offer potentially more reliable results. …”
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Pemetaan Spasial Kekeringan Menggunakan Metode Standardized Precipitation Index (SPI) di DAS Blega, Kabupaten Bangkalan by Donny Harisuseno, Muhammad Amar Sajali, Winnie Meilinda Dewi

“…Pola hubungan antara indeks kekeringan bulanan dengan tingkat kekeringan rentang 1992 - 2021 menunjukkan tingkat kekeringan "Amat Sangat Kering" terjadi secara berkala setiap empat tahun. …”
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Comparative (Computational) Analysis of the DNA Methylation Status of Trinucleotide Repeat Expansion Diseases by Mohammadmersad Ghorbani, Simon J. E. Taylor, Mark A. Pook, Annette Payne

“…Using the J48 algorithm of WEKA analysis, we identified that two patterns are all that is necessary to classify our three regions CCGG* which is found in VM and not in AM regions and AATT* which distinguished between NM and VM + AM using proportional frequency. …”
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Primary Adrenal Failure due to Antiphospholipid Syndrome by Murat Sahin, Ayten Oguz, Dilek Tuzun, Serife Nur Boysan, Bülent Mese, Hatice Sahin, Kamile Gul

“…The patient was diagnosed with antiphospholipid syndrome after the detection of venous thrombosis, thrombocytopenia, elevated aPTT, and anticardiolipin antibody levels. Anticoagulation treatment was started for antiphospholipid syndrome. …”
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