Characterization of Various Plant-Produced Asphalt Concrete Mixtures Using Dynamic Modulus Test by Muhammad Irfan, Asad S. Waraich, Sarfraz Ahmed, Yasir Ali

“…This research characterizes the performance of various plant-produced asphalt concrete mixtures by dynamic modulus |E∗| test using asphalt mixture performance tester (AMPT). Marshall designed specimens of seven different mixtures were prepared using the Superpave gyratory compactor and subjected to sinusoidal compressive loading at various temperatures (4.4 to 54.4°C) and loading frequencies (0.1 to 25 Hz). …”
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Comparison of effectiveness of aspirin, clopidogrel and cilostazol monotherapy treatment in ischaemic stroke by Endarwati Tuti, Kurniasih Khamdiyah Indah, Fauziah Fauziah, Sunarti Sunarti, Agus Salim Muh.

“…Outperforming aspirin and cilostazol, clopidogrel (75 mg) were the most widely used medication (38.6%) and produced the best results, considerably enhancing PT (1 s), APTT (0.872 s), and lowering leukocytes (2.956) and platelets (3.035). …”
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Peranan motivasi dan disiplin kerja pada produktivitas karyawan by Mukrodi Mukrodi

“…Produktifitas merupakan aktifitas yang dilakuakn seseorang untuk mendapatkan output dari proses yang sudah dilakukan Produktifitas amat sangat penting untuk dilakukan dengan baik dan maksimal agar hasil kerja yang didapat tercapai harapan perusahaan. …”
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Effect of Liquid Nitrogen Cooling and Heating on Mechanical Properties and Acoustic Emission Characteristics of Coal by Yuhe Cai, Yi Xue, Faning Dang, Linchao Wang, Xue Li, Shanjie Su, Shengcheng Wang, Xinyu Liang, Shaowei Zhang

“…This paper investigates two coal types from the Jiaozuo area, which were subjected to liquid nitrogen cyclic treatment using Brazil’s indirect tensile testing (BITT). The study analyzes the characteristics of acoustic emission (AE) and crack formation during the coal cracking process and evaluates the impact of freeze-thaw treatment with liquid nitrogen cycles on the mechanical properties of coals. …”
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Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms by Irina Tiganova, Yulia Zhizhimova, Eteri Tolordava, Elena Makarova, Natalia Shevlyagina, Alexander Barmashov, Maria Baryshnikova, Vladimir Zhukhovitsky, Yulia Romanova

“…Methods: Photodynamic inactivation of eucaryotic cells was obtained with MTT test, and with plating of planktonic bacteria P. aeruginosa and biofilms after disrupting. …”
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CD4+CD25+调节性T细胞的体外扩增

“…培养第7、14、21天收集细胞计数，第21天收集细胞行流式细胞术三标法检测CD4、CD25、FOXP3的表达。MTT法检测体外扩增的CD4+CD25+Treg对自体和异体的CD4+T细胞增殖的抑制作用。 【结果】 三组细胞均有明显的扩增，加rapamycin培养的CD4+CD25+Treg细胞的扩增率比不加rapamycin扩增倍数低，但细胞的纯度明显增加。…”
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Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition by Min Jung Kim, Ye Jin Yang, Ga-Yul Min, Ji Woong Heo, Jae Dong Son, Young Zoo You, Hun Hwan Kim, Gon Sup Kim, Hu-Jang Lee, Ju-Hye Yang, Kwang Il Park

“…Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyphenol and flavonoid content were determined. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay measured antioxidant activity. …”
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Epidermal Growth Factor Promotes E6 and CCL-81 Vero Cells Proliferation Under Serum-Free Condition by Diena Delaiah, Ahmad Aswin, Helen Susilowati, Andi Yasmin Wijaya, Firdausy Kurnia Maulana, Diyantoro Diyantoro, Watchareewan Rodprasert, Yulianna Puspitasari, Yeni Dhamayanti, Suryo Kuncorojakti

“…The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. …”
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Bis(7)-harmine derivatives as potential multi-target anti-Alzheimer agents by Hongtao Du, Hongtao Du, Hongtao Du, Fang Ma, Fang Ma, Yuanyuan Cao, Miaoyan Bai, Xinyi Gao, Ziyi Yang, Yang Xu, Yan Yan, Yan Yan

“…The cytotoxicity was evaluated using the MTT assay. The Surflex-Dock program in Sybyl-X2.0 Software was employed for molecular docking.ResultsIn vitro studies revealed that numerous synthesized compounds exhibited potent inhibitory activity against hAChE, and hMAO-B (IC50 < 1 μM), as well as Aβ1−42 aggregation (IC50 < 20 μM). …”
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Acute Consumption of Bordo Grape Juice and Wine Improves Serum Antioxidant Status in Healthy Individuals and Inhibits Reactive Oxygen Species Production in Human Neuron-Like Cells by Cristiane Copetti, Fernanda Wouters Franco, Eduarda da Rosa Machado, Marcela Bromberger Soquetta, Andréia Quatrin, Vitor de Miranda Ramos, José Cláudio Fonseca Moreira, Tatiana Emanuelli, Cláudia Kaehler Sautter, Neidi Garcia Penna

“…To evaluate differences in cellular response, intracellular reactive species production (DCFH-DA) and metabolic mitochondrial viability (MTT) were assessed after exposure of human neuron-like cells (SH-SY5Y) to juice or wine. …”
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Hybrid peptides inspired by the RWQWRWQWR sequence inhibit cervical cancer cells growth in vitro by Natalia Ardila-Chantré, Claudia Marcela Parra-Giraldo, Yerly Vargas-Casanova, Andrea Carolina Barragán-Cardenas, Ricardo Fierro-Medina, Zuly Jenny Rivera-Monroy, Jhon Erick Rivera-Monroy, Javier Eduardo García-Castañeda

“…In vitro cytotoxicity of hybrid peptides in human cervical cancer cells lines HeLa and Ca Ski was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: All hybrid peptides were obtained with high purity and the experimental mass corresponds with the theoretical mass. …”
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Sodium Butyrate: A Multifaceted Modulator in Colorectal Cancer Therapy by Alexandra Laura Mederle, Alexandra Semenescu, George Andrei Drăghici, Cristina Adriana Dehelean, Nicolae-Valentin Vlăduț, Dragoş Vasile Nica

“…Cell viability, confluence, number, morphology, and nuclear integrity were assessed using MTT and imaging assays. RT-PCR was used to determine changes in the expression of critical pro-apoptotic players (<i>BAX</i>, <i>CASP3</i>, <i>PUMA</i>, <i>TP53</i>), anti-apoptotic facilitators (<i>BCL</i>-2, <i>MCL-1</i>), cell division regulators (<i>PCNA</i>, <i>Ki-67</i>, <i>CDKN</i>1), and inflammation genes (<i>NF-κB</i>). …”
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ABT737通过延迟宫颈癌细胞放射后DNA损伤修复及诱导凋亡而增敏放疗

“…【目的】 探讨类似BH-3的小分子物质ABT737诱导增敏放疗的作用及其分子机制?【方法】噻唑蓝法(MTT)检测不同浓度ABT737对HeLa细胞的生长抑制作用;细胞克隆形成实验检测ABT737联合放疗对放疗的增敏作用;免疫荧光法检测γ-H2AX观察DNA损伤修复情况;流式细胞术检测细胞凋亡;免疫印迹法检测Caspase-3?…”
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异荭草苷体内外抗呼吸道合胞病毒活性研究

“…摘 要: 【目的】 研究清热消炎中草药淡竹叶中异荭草苷的体内外抗病毒活性,并初步探讨其作用机制? 【方法】 采用MTT法测定异荭草苷的细胞毒性,并以异荭草苷的最大无毒浓度为起始浓度,采用空斑减数法测定其体外抗RSV活性,计算其抗病毒的选择性指数(SI值);继而采用空斑减数法,研究异荭草苷对RSV复制周期不同阶段的影响,采用实时定量PCR法,检测异荭草苷对病毒蛋白mRNA合成的影响;选取3周龄BALB/c雄性小鼠研究异荭草苷体内抗RSV活性,小鼠灌胃给药4 d,经鼻腔滴入RSV病毒液造模,继续灌胃给药3 d,取小鼠肺组织称重?…”
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هسپریدین، یک عامل مهارکننده رشد سلول‌های سرطانی از طریق مسیر آپوپتوز by امیر رئوفی, مینا آبرودی, غزاله داداشی زاده, سعیده سادات شبیری, آذر حسینی, امید غلامی, رضا عطائی دیسفانی, داود مهدیان, مریم خسروجردی

“…در این تحقیق، تأثیرات ضدسرطانی هسپریدین بر رده‌های سلولی سرطانی مختلف ازجمله تومور مدولاری آدرنال (فئوکروموسیتوم) (PC12)، آدنوکارسینوم کولون (SW48)، سرطان پروستات (DU145) و کارسینوم سلول سنگفرشی مری (KYSE30) بررسی شد.مواد و روش­ها: سلول‌ها با غلظت‌های مختلف هسپریدین (100-0.75 میکرومولار) به ‌مدت 24، 48 یا 72 ساعت تیمار شدند و بقای سلول‌ها با روش MTT ارزیابی شد. بیان ژن‌های Bax ،Bcl-2  و کاسپاز3 نیز با استفاده از RT-PCR اندازه‌گیری شد. …”
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Enhancing elemental release and antibacterial properties of resin-based dental sealants with calcium phosphate, bioactive glass, and polylysine by Phatpicha Lertwisitphon, Yotsavee Worapasphaiboon, Nichapa Champakanan, Arnit Toneluck, Parichart Naruphontjirakul, Anne M. Young, Rattapha Chinli, Phoom Chairatana, Supanan Sucharit, Piyaphong Panpisut

“…The cell viability of mouse fibrosarcoma cells exposed to the extract was assessed via an MTT assay (n = 3). Additionally, the inhibition of S. mutans biofilm was tested (n = 3). …”
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Fermented sugarcane juice-derived probiotic Levilactobacillus brevis RAMULAB54 enhances lipid metabolism and glucose homeostasis through PPAR-γ activation by V. B. Chandana Kumari, Ramith Ramu, Sujay S. Huligere, Shashank M. Patil, Shivasharanappa Nayakvadi, Sharath Bijoor, Uma Venkateswaran Manjappara, Mohammad Z. Ahmed, Ling Shing Wong

“…Organic acid interactions with PPAR-γ were evaluated via molecular docking, while cytotoxicity and adipocyte differentiation in 3T3-L1 cells were tested using MTT assays, Oil Red O staining, triglyceride quantification, and qRT-PCR. …”
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Lactate Dehydrogenase-A-Forming LDH5 Promotes Breast Cancer Progression by Fang T, Hu L, Chen T, Li F, Yang L, Liang B, Wang W, Zeng F

“…The in vitro proliferation, migration and invasion potentials of BC cells were evaluated using MTT assays, colony formation, wound-healing assay, matrix metalloproteinase assays and transwell assays, respectively. …”
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Synthesis, characterization and biological profile of some new dihydropyrimidinone derivaties by Madiha Kanwal, Humaira Nadeem, Sumra Malik, Saima Maqsood

“…The cytotoxicity profile of the synthesized derivatives was done via in-vitro MTT assay. Results: All newly synthesized derivatives were confirmed via the multiple spectroscopic analysis techniques. …”
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Agathisflavone Inhibits Viability and Modulates the Expression of miR-125b, miR-155, IL-6, and Arginase in Glioblastoma Cells and Microglia/Macrophage Activation by Karina Costa da Silva, Irlã Santos Lima, Cleonice Creusa dos Santos, Carolina Kymie Vasques Nonaka, Bruno Solano de Freitas Souza, Jorge Mauricio David, Henning Ulrich, Ravena Pereira do Nascimento, Maria de Fátima Dias Costa, Balbino Lino dos Santos, Silvia Lima Costa

“…Agathisflavone (5–30 μM) induced a dose- and time-dependent reduction in the viability of both human GL-15 and rat C6 cells, as determined by the MTT test, and reduced cell migration, as determined by cell scratch assay. …”
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