Natural Polymer Derivative-based pH responsive Nanoformulations Entrapped Diketo-tautomers of 5-fluorouracil for Enhanced Cancer Therapy by Anbazhagan Thirumalai, Koyeli Girigoswami, Karthick Harini, Venkatakrishnan Kiran, Pazhani Durgadevi, Agnishwar Girigoswami

“…Experimental approach: The investigation encompassed physicochemical characterization, encapsulation efficiency, 5-FU release kinetics at pH 2.2 and 7.4, cell viability assessment via MTT assay in V79 cells, and in vitro anticancer efficacy in the A375 cell line. …”
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生长激素上调性激素抑制下青春期大鼠生长板原代软骨细胞表皮生长因子的表达

“…【目的】 研究生长激素(GH)对性激素抑制下的离体青春期大鼠生长板软骨细胞表皮生长因子(EGF)mRNA表达及其蛋白合成65380;分泌的影响65377;【方法】 3周龄雌性SD大鼠开始注射促性腺激素释放激素拟似物(GnRHa)至7周龄,随后分离培养5 ~ 8只大鼠胫骨生长板原代软骨细胞,分为时效组和量效组65377;采用四氮甲基唑蓝(MTT)以及免疫组化法测定增殖细胞核抗原(PCNA),观察不同剂量65380;不同干预时间的生长激素对性激素抑制下的离体青春期大鼠生长板软骨细胞增殖的影响65377;用RT-PCR法检测软骨细胞EGF mRNA的表达,酶联免疫吸附法(ELISA)测定EGF的表达65377;【结果】 生长激素以时效和量效作用方式对雌激素受抑的离体青春期大鼠生长板软骨细胞增殖呈双相型影响65377;GH作用6 h后软骨细胞的EGF mRNA表达显著增加(与0 h比较P < 0.05),且从6 h到24 h间存在明显的时间依赖性(组间P < 0.05),在18 h达到最高峰65377;GH作用12 h后软骨细胞分泌入培养基中的的EGF表达显著增加(与0 h比较P < 0.05),且从12 h到48 h间存在明显的时间依赖性(组间P < 0.05),在36 h达到最高峰65377;量效关系显示50 ng/mL浓度的GH开始能显著增加培养基中EGF mRNA和蛋白的表达,且从50 ng/mL至1 000 ng/mL间存在明显的浓度依赖性(组间P < 0.05),在100 ng/mL时表达达到最高峰65377;【结论】 生长激素能促进性激素抑制下的离体青春期大鼠生长板软骨细胞的增殖及EGF mRNA和蛋白的表达65377;…”
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Inhibitory effects of limonin on the proliferation of non-small cell lung cancer cells via targeting S-phase kinase-associated protein 2 by Zhu Jiaxian, Zhang Mengting, Li Shengqing, Liu Yongqiang

“…Methods To investigate the inhibitory effects of limonin on NSCLC cell activity, the MTT assay was employed. Then, flow cytometry was used to detect the cell cycle and apoptosis, while colony formation assay assessed the low-density proliferation ability of NSCLC cells. …”
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Effects of the Highly COX-2-Selective Analgesic NSAID Etoricoxib on Human Periodontal Ligament Fibroblasts during Compressive Orthodontic Mechanical Strain by Christian Kirschneck, Erika Calvano Küchler, Michael Wolf, Gerrit Spanier, Peter Proff, Agnes Schröder

“…We then determined gene and/or protein expression of COX-2, IL-6, PG-E2, RANK-L, OPG, ALPL, VEGF-A, P4HA1, COL1A2, and FN1 via RT-qPCR, ELISA, and Western blot analyses as well as apoptosis, necrosis, cell viability, and cytotoxicity via FACS, MTT, and LDH assays. In addition, hPDL fibroblast-mediated osteoclastogenesis was assessed by TRAP staining in coculture with RAW267.4 cells for another 72 h. …”
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Blue-LED assisted Photodegradation kinetics of rhodamine-6G dye, enhanced anticancer activity and cleavage of plasmids using Au-ZnO nanocomposite by Pilavadi Thangamuniyandi, Devan Umapathy, Loganathan Nagarajan, Antony Joseph Velanganni Arockiam

“…Further, the blue-LED assisted anti-cancer studies (MTT assay) using 0.015-Au-ZnO towards human lung cancer cells (A549), breast cancer cells (SKBr3) show high anti-proliferation rate and low cytotoxicity against healthy human embryonic kidney cells (HEK-293) with an IC50 value of 65, 53 and 124 μg/mL respectively. …”
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靶向调控非小细胞肺癌的作用机制 by 谢传华, 陈海龙, 黄萍, 郭守俊, 邱伊连, 王硕, 潘宜云, 胡志苹

“…【方法】培养将非小细胞肺癌A549 细胞系分为 5 组 NC mimic 组（miR-204-3p 过表达阴性对照组）、miR-204-3p mimic 组（miR-204-3p 过表达组）、oe-NC 组（EphB2 过表达阴性对照组）、oe-EphB2 组（EphB2 过表达组）、miR-204-3p mimic+oe-EphB2 组（miR-204-3p 与EphB2 均过表达组）。MTT、流式细胞术、Transwell、划痕实验分别检测细胞增殖、周期、凋亡、侵袭、迁移情况。双荧光素酶报告分析 miR-204-3p 与 EphB2 靶向关系。…”
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Tryptanthrin exerts anti-breast cancer effects both in vitro and in vivo through modulating the inflammatory tumor microenvironment by Zeng Qingfang, Luo Cairong, Cho Junlae, Lai Donna, Shen Xiangchun, Zhang Xiaoyan, Zhou Wei

“…Human breast adenocarcinoma MCF-7 cells were used to assess the antitumor effect of tryptanthrin in vitro. MTT assay and colony formation assay were carried out to monitor the antiproliferative effect of tryptanthrin (1.56~50.0 μmol L−1) on inhibiting the proliferation and colony formation of MCF-7 cells, respectively. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…The effects of USP39 depletion on tumor progression were tested using doxycycline-inducible USP39 knockdown and rescue lung adenocarcinoma cells both in vitro and in vivo by performing MTT, colony formation, EdU staining, transwell and tumor xenograft model experiments. …”
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Exploring the anticancer potential of extracts and compounds from the heartwood of Cotinus coggygria Scop. wild growing in Serbia by Ivana Pašić, Miroslav Novaković, Vele Tešević, Slobodan Milosavljević, Nina Petrović, Tatjana Stanojković, Ivana Z. Matić

“…Cytotoxicity was evaluated using MTT assay. The effects on cell cycle and cell death were investigated by flow cytometry. …”
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Runx2-树突状细胞基因疫苗诱导特异性细胞免疫杀伤 三阴乳腺癌

“…PCR和Westernblot检测各组转染效率;将各组转染细胞与T淋巴细胞共培养,ELISA检测IL-12?IFN-γ的分泌量,MTT检测疫苗对三阴乳腺癌的特异性杀伤作用?【结果】 PCR结果示Runx2在MDA-MB-231?…”
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The frequency of mutations in the penA, mtrR, gyrA and parC genes of Neisseria gonorrhoeae, the presence of tetM gene and antibiotic resistance/susceptibility: a systematic review and meta-analyses by Ana Clara Mendes, Renan Pedra de Souza, Diana Bahia

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Pengaruh Terapi Pijat Terhadap Kenaikan Berat Badan Bayi Prematur di RSUP. Dr. M. Djamil Padang by Yori Rahmi, Wedya Wahyu, Eliza Anas

“…According to the American Association of Massage Therapy (AMTA), massage is defined as the manipulation of soft tissue using hands or body to hold, move, or apply stress manually. …”
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Evaluación del Impacto de la Implementación de Techos Verdes en el Dimensionamiento de Sistemas de Alcantarillado Urbano by Belén Shakira Tapia Montero, Daysi Gabriela Barros Tuapante, María Gabriela Soria Pugo

“…Para estimar la escorrentía, se utilizaron modelos como el Número de Curva, Horton y Green AMPT, considerando variables como clima, sustrato y pendiente. …”
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Evaluasi peran pemerintah dalam menentukan angka stunting berdasarkan perpres 72 tahun 2021 by Suci Rahmadani, Syofiati Lubis

“…Masalah stunting menjadi target yang amat serius karena berdampak pada kecerdasan dan produktifitas bangsa indoneisa di masa mendatang. …”
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Effectiveness of late and very late antivenom administration on recovery from snakebite-induced coagulopathy in French Guiana: a population-based studyResearch in context by Jean Marc Pujo, Stephanie Houcke, Guy Roger Lontsi Ngoulla, Vivian Laurent, Boubacar Signaté, Rémi Mutricy, Alexis Frémery, Flaubert Nkontcho, Ibtissem Ben Amara, José María Gutiérrez, Dabor Resiere, Hatem Kallel

“…Meanwhile, the time from AV to normal INR or normal aPTT was similar in patients receiving very late AV compared to patients receiving late AV (Log-Rank = 0.722 and 0.740, respectively). …”
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Direct oral anticoagulants (DOACs): From the laboratory point of view by Margetić Sandra, Goreta Sandra Šupraha, Ćelap Ivana, Razum Marija

“…Although DOACs have an impact on screening haemostasis assays (prothrombin time, PT; activated partial thromboplastin time, aPTT; and thrombin time, TT), these tests are not appropriate for quantifying drug levels. …”
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Hyaluronan Immobilized Polyurethane as a Blood Contacting Material by Feirong Gong, Yue Lu, Hui Guo, Shujun Cheng, Yun Gao

“…Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy (RS), scanning electron microscopy (SEM), and water contact angle measurement were used to confirm the surface changes at each step of treatment, both in morphologies and chemical compositions. APTT and PT results showed that HA immobilization could prolong the blood coagulation time, thus HA-immobilized polyurethane (PU-HA) exhibited improved blood compatibility. …”
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Preparation of Polymeric Nanomaterials Using Emulsion Polymerization by Satyajeet Sahoo, Anitha Gopalan, S. Ramesh, P. Nirmala, G. Ramkumar, S. Agnes Shifani, Ram Subbiah, J. Isaac JoshuaRamesh Lalvani

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Evaluation of the effects of increasing doses of meloxicam on hematological, biochemical and hemostatic blood parameters in sheep by Fulya Altınok Yipel, Hüseyin Güngör

“…In addition, hemogram (RBC, MCV, MCH, MCHC, HCT, WBC, HGB, RDW, MPV, PDW, PCT, PLT) and biochemical parameters (AST, ALT, ALP, GGT, TP, UREA, CREA, CHOL, TRIG, ALB) ) and the levels of coagulation parameters (PT, APTT, INR, FIB) were determined.<p> <b>Results: </b>In groups; Differences in biochemical parameters other than UREA and parameters of RBC, HCT, MCV, MCHC, MPV were statistically significant (p<0,05). …”
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Pronunciation learning attitudes: The case of EFL undergraduate students in Indonesia by Toni Indrayadi, Herri Mulyono, Sri Kusuma Ningsih, Amirul Mukminin

“…An explanatory sequential mixed-method approach, integrating questionnaires of Gardner 1985’s Attitude Motivation of Test Battery (AMTB) and Elliot 1995’s Pronunciation Attitude Inventory (PAI) and interviews, was employed in this study. …”
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