„Elmélet vs. gyakorlat” a performativitás felől. Adalékok a praxis-vitához by Lóránt Kicsák

“…Azonban nem értem, miért lenne irreleváns, ki, mit gondol a filozófiáról. Persze nem lényegmeghatározását kell adnunk, már csak azért sem, mert a filozófiára szintén érvényes az, ami az emberre: „essentiája az existentiájában rejlik”. …”
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Karinių pratybų poveikis profesionalių karių miego kokybei by Ligita Mažeikė, Arminas Vareika

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Phytochemical, Cytotoxic, and Antimicrobial Evaluation of the Fruits of Miswak Plant, Salvadora persica L. by Mohammed Al Bratty, Hafiz A. Makeen, Hassan A. Alhazmi, Sohier M. Syame, Ashraf N. Abdalla, Husham E. Homeida, Shahnaz Sultana, Waquar Ahsan, Asaad Khalid

“…The cytotoxic properties of the ethanol extract were investigated by MTT assay against the breast MCF7, ovary A2780, and colon HT29 cells. …”
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The Accuracy and Precision of the Continuously Stored Data from Flash Glucose Monitoring System in Type 2 Diabetes Patients during Standard Meal Tolerance Test by Rengna Yan, Huiqin Li, Xiaocen Kong, Xiaofang Zhai, Maoyuan Chen, Yixuan Sun, Lei Ye, Xiaofei Su, Jianhua Ma

“…Weighted least squares regression analysis resulted in high agreement between the FGM sensor glucose and VBG readings. The overall MTT results showed that FGM was lower than actual VBG, with MAD of 22.1 mg/dL (1.2 mmol/L). …”
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CXCR4 promotes tumor stemness maintenance and CDK4/6 inhibitors resistance in ER-positive breast cancer by Qiangfeng Shi, Wang Yang, Yiye Ouyang, Yujie Liu, Zijie Cai

“…The effects of CXCR4 knockdown and overexpression via siRNAs and plasmids transfection were examined by mammosphere formation, RT-qPCR, flow cytometry, MTT and colony formation assays. The regulation mechanisms were analyzed by RT-qPCR, western blotting and immunofluorescence experiments. …”
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二甲双胍通过增强M146L细胞IDE表达促进胞内Aβ降解 by 谢婷, 徐采利, 唐姣玲, 郭开华

“…目的探讨二甲双胍（Met）作用AD细胞模型M146L的淀粉样蛋白-β（Aβ）病理改善作用及其潜在机制。方法MTT比色法检测不同浓度Met对细胞活力的影响；Western Blotting检测Met处理后Aβ₄₂、生成Aβ相关的淀粉样蛋白前体（APP）、β分泌酶（BACE），以及Aβ降解相关的胰岛素降解酶（IDE）、脑啡肽酶（NEP）、轻链蛋白3 Ⅱ/Ⅰ （LC3Ⅱ/Ⅰ）的表达量变化；免疫荧光检测Met处理后Aβ₄₂、IDE的表达；Western Blotting检测IDE的特异性抑制剂杆菌肽（Bac）和Met联合处理后IDE和Aβ₄₂的表达情况。…”
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UPLC-ESI-MS/MS Profile and Antioxidant, Cytotoxic, Antidiabetic, and Antiobesity Activities of the Aqueous Extracts of Three Different Hibiscus Species by Hanan M. Al-Yousef, Wafaa H. B. Hassan, Sahar Abdelaziz, Musarat Amina, Rasha Adel, May A. El-Sayed

“…The results showed that aqueous extract of Hibiscus calyphyllus had the highest activity as an antioxidant agent (SC50 = 111 ± 1.5 μg/mL) compared with ascorbic acid (SC50 = 14.2 ± 0.5 μg/mL). MTT assay was used to evaluate cytotoxic activity compared to cisplatin. …”
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Antiplasmodial and Cytotoxic Activities of Extracts of Selected Medicinal Plants Used to Treat Malaria in Embu County, Kenya by Bibianne Waiganjo, Gervason Moriasi, Jared Onyancha, Nelson Elias, Francis Muregi

“…Finally, cytotoxic effects and safety of the studied plant extracts were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) rapid calorimetric assay technique. The water and methanolic extracts of T. brownii and S. africana and dichloromethane extracts of E. abyssinica, S. pinnata, and T. diversifolia leaves revealed high in vitro antiplasmodial activities (IC50≤10 μg/ml). …”
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Corneal protective effects of a new ophthalmic formulation based on vitamin B12 and sodium hyaluronate by Francesca Lazzara, Federica Conti, Grazia Maugeri, Velia D’Agata, Velia D’Agata, Ludovica Sotera, Claudio Bucolo, Claudio Bucolo

“…Cell viability, cytotoxicity, ROS production, and mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β) were assessed by MTT, LDH, 2′,7′–dichlorofluorescein diacetate (DCFDA) assays and Real-time PCR, respectively. …”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…To investigate the therapeutic potential of BET/EP300 inhibition, we evaluated the antitumor activity of three compounds in vitro via MTT, apoptosis and cell cycle assays. The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. …”
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3D nanocomposites of β-TCP-H3BO3-Cu with improved mechanical and biological performances for bone regeneration applications by Sarvesh Kumar Avinashi, Rajat Kumar Mishra, Shweta, Saurabh Kumar, Amreen Shamsad, Shama Parveen, Surajita Sahu, Savita Kumari, Zaireen Fatima, Sachin Kumar Yadav, Monisha Banerjee, Monalisa Mishra, Neeraj Mehta, Chandki Ram Gautam

“…Moreover, the mechanical study showed excellent compressive strength (188 MPa), a high Young’s modulus (2.84 GPa), and elevated fracture toughness (9.11 MPa.m1/2). An in vitro study by MTT assay on osteoblast (MG-63) cells demonstrated no or minimal cytotoxicity at the higher concentration, 100 µg/ml after 24 h and it was found a more pronounced result at 20 µg/ml on increasing the concentration of Cu nanoparticles after incubating 72 h. …”
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荜茇酰胺通过诱导口腔鳞癌细胞自噬抑制增殖 by 许小鸿, 郅程, 袁忠民

“…方法体外培养口腔鳞状细胞癌Cal27及UM1细胞株，经不同浓度的荜茇酰胺处理后，MTT比色法检测荜茇酰胺对增殖的影响，蛋白质印迹法检测胞内微管相关蛋白轻链 3-I、3-Ⅱ （LC3-I、 LC3-Ⅱ）、Beclin1、p62、雷帕霉素靶蛋白（mTOR）及ribosomal S6 （S6） 的表达或活性变化；用自噬抑制剂3-Methyladenine（3-MA）或者Bafilomycin A1（BAFA1）和荜茇酰胺共处理细胞后，检测LC3-Ⅱ、p62表达和细胞活力。…”
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Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells by Peihua Zhang, Jin Li, Yawei Qi, Xudong Tang, Jianfeng Duan, Li Liu, Zeyong Wu, Jie Liang, Jiangfeng Li, Xian Wang, Guofang Zeng, Hongwei Liu

“…Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively. Cell cycle progression was determined by flow cytometry. …”
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Ultrasound super-resolution imaging for non-invasive assessment of microvessel in prostate lesion by Xin Huang, Huarong Ye, Yugang Hu, Yumeng Lei, Yi Tian, Xingyue Huang, Jun Zhang, Yao Zhang, Bin Gui, Qianhui Liu, Ge Zhang, Qing Deng

“…CEUS parameters of time-intensity curve (TIC): arrival time (AT), rising time (RT), time to peak (TTP), peak intensity (PKI), falling time (FT), mean transit time (MTT), ascending slope (AS), descending slope (DS), D/A slope ratio (SR), and area under the TIC (AUC). …”
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FAK抑制剂对肝癌HCC-LM3细胞骨架重排和侵袭的作用机制 by 姚红兵, 肖芳, 郭威, 吴嘉兴, 文雪霖, 蒋建晖, 华赟鹏

“…方法HCC-LM3细胞分为Control组、CT-707低剂量（1.5 μmol/L）组、CT-707中剂量（3 μmol/L）组、CT-707高剂量（6 μmol/L）组，采用Transwell小室实验、细胞划痕、MTT实验分别测定细胞侵袭、迁移能力及细胞活力；采用RT-qPCR和Western blot分别检测Palladin、Vimentin、MMP2和MMP9 mRNA及蛋白的表达；Western blot检测p-FAK、FAK、p-PI3K、PI3K、p-Akt及Akt蛋白的表达。…”
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Mild heat stress promotes the differentiation of odontoblast-like MDPC-23 cells via yes-associated protein by Peiqi Liu, Zhen Li, Hui Zhang, Yijie Wang, Yuxin Liao, Yi Guo, Chenxu Wang, Yuanwu Zou, Rui Zou, Lin Niu

“…Cell viability was assessed using the MTT assay. The expression and nucleoplasmic ratio of the yes-associated protein (YAP) were examined by western blotting and immunofluorescence. …”
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Exosomes and their distinct integrins transfer the characteristics of oxaliplatin- and 5-FU-resistant behaviors in colorectal cancer cells by Zeynab Vakili-Ghartavol, Hoda Deli, Amir Shadboorestan, Roxana Sahebnasagh, Elahe Motevaseli, Mohammad Hossein Ghahremani

“…The effects of exosomes on parental cells were examined using various methods, including MTT assay for proliferation, wound healing assay for migration, flow cytometry for cell cycle and apoptosis analysis, Matrigel-coated transwell inserts for invasion, and western blot for integrin expression evaluation. …”
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Structural Properties, Cytotoxicity, and Anti-Inflammatory Activity of Silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2-Mercaptobenzothiazole by L. Kyros, N. Kourkoumelis, M. Kubicki, L. Male, M. B. Hursthouse, I. I. Verginadis, E. Gouma, S. Karkabounas, K. Charalabopoulos, S. K. Hadjikakou

“…The in vitro testing of cytotoxic activity of 1–5 against leiomyosarcoma cancer cells (LMS), were evaluated with Trypan Blue and Thiazolyl Blue Tetrazolium Bromide or 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assays. The flow cytometry assay for complex 1 and showed that at 15 μM of 1, 62.38% of LMS cells undergo apoptosis, while 7% of LMS cells undergo cell necrosis. …”
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Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4 by Rongyu Shi, Ning Zhang, Han Li, Hu Zhong, Chengcheng Zhong, Wei Du, Xi Yang

“…Functional analyses were conducted using MTT, 5-ethynyl-2ʹ-deoxyuridine, transwell, and sphere formation assays, respectively. …”
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不同病毒载量的乙型肝炎病毒在不同ALT状态下对肝星状细胞增殖、细胞周期及细胞分泌的影响 by 高鹏, 刘才周, 朱陇东, 李俊峰, 于海涛, 姚立琼

“…方法分层随机选取不同ALT状态下不同病毒载量的慢性乙型肝炎的患者132例，作为干扰血清。经干扰血清干预HSC后，分别用MTT法检测HSC增殖， 流式细胞仪检测HSC细胞周期， RT-PCR 和免疫印迹分别检测<italic>TGF-β1、Smad3、 Smad7、α-SMA、collagen Ⅰ、collagen Ⅲ</italic> mRNA 及蛋白的表达，同时对ALT ≥2UNL的慢性乙肝患者抗病毒治疗随访24周，对比分析治疗前后患者干扰血清对肝星状细胞的影响。…”
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