Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials by Ratri Maya Sitalaksmi, Rizkipriyanto Azharpratomo, Shafira Ninditya Irsan, Primanda Nur Rahmania, Sisca Meida Wati, Syafira Salsabila Adam, Kavanila Bilbalqish, Khairul Anuar bin Shariff, Harry Laksono, Agus Dahlan, Devi Rianti

“…Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. …”
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The in vitro anticancer effects of FS48 from salivary glands of Xenopsylla cheopis on NCI-H460 cells via its blockage of voltage-gated K+ channels by Xiong Weichen, Fan Huizhen, Zeng Qingye, Deng Zhenhui, Li Guanhui, Lu Wancheng, Zhang Bei, Lai Shian, Chen Xin, Xu Xueqing

“…The present study aims to reveal the effects of FS48 on the Kv channels and the NCI-H460 human lung cancer cells through patch clamp, MTT, wound healing, transwell, gelatinase zymography, qRT-PCR and WB assays. …”
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Towards targeted cancer therapy: Synthesis, characterization, and biological activity of a new Cu(II)-ibuprofen-2,2′-dipyridylamine metal complex by Luís P.G. Monteiro, A. Gomes, C. Silva, Ricardo F. Mendes, Filipe A. Almeida Paz, Dmitry Chernyshov, J. Rocha, F. Martel, T.M. Santos, B.J.M. Leite Ferreira

“…Finally, the copper compound exhibited promising in vitro antitumoral activities against human breast cancer cell lines (MCF-7 and MDA-MB-231), as evaluated by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay (although a similar cytotoxic effect against a non-tumoral epithelial cancer cell line, MCF-12A, was found), and increased oxidative stress levels as assessed by the TBARS (thiobarbituric acid reactive substances) assay and by evaluating glutathione levels. …”
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Persea americana Glycolic Extract: In Vitro Study of Antimicrobial Activity against Candida albicans Biofilm and Cytotoxicity Evaluation by D. Jesus, J. R. Oliveira, F. E. Oliveira, K. C. Higa, J. C. Junqueira, A. O. C. Jorge, G. N. Back-Brito, L. D. Oliveira

“…The cytotoxicity of the P. americana extract was evaluated on macrophages by MTT assay. The MIC of the extract was 6.25 mg/mL and with 12.5 mg/mL there was elimination of 100% of planktonic cultures. …”
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Impact of Amarogentin on Gastric Carcinoma Cell Multiplication, Apoptosis and Migration via circKIF4A/miR-152-3p by Zhi Tan, Weining Wang, Jin Peng, Zhen Zhou, Jia Pan, Aiming Peng, Hui Cao, Wenling Fan

“…Cell multiplication, clone formation, as well as apoptosis and migration measurements were made by MTT, plate clone formation, flow cytometry and Transwell assays, respectively; Double luciferase reporter assay was performed for targeting relationship identification between circKIF4A and miR-152-3p; Western blots were carried out to measure Bax and Bcl-2 protein levels. …”
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Upregulation of GSTP1 mediated by chimeric TFE3 promotes TFE3-tRCC progression by targeting JNK signaling pathway by Weixu Chen, Mengtong Wu, Lin Du, Changhua Fang, Hao Wang, Wendi Wang, Chengwei Zhang, Hongqian Guo, Gutian Zhang

“…The location of ASPL-TFE3 fusion protein was analyzed by immunofluorescence. MTT and colony formation assays were used to detect cell proliferation. …”
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基因干扰Sirtuin3后对缺氧预处理的影响

“…成功构建空白载体（empty vector）及SIRT3-/-稳定细胞株后，将两种细胞分别分为对照组（control）；缺氧预处理+缺氧组(Hyp+OGD)；单纯缺氧组(OGD)；MTT测定细胞活性。ATP荧光检测试剂盒测定胞内的ATP水平。Western Blot法测定各组细胞内SIRT3、过氧化物酶体增生激活受体-γ的共刺激因子-1α(PGC-1α)，锰超氧化物歧化酶（MnSOD）的蛋白表达水平。…”
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Effect of Qingjie Fuzheng Granules on Proliferation, Apoptosis and Migration of Colorectal Cancer SW620 Cells by Xiaoqin ZHU, Hong YANG, Bin HUANG, Lihui WEI, Jiumao LIN

“…The inhibitory effect of QFG on cell proliferation was detected by MTT assay after 24 h and 48 h. The morphology of SW620 cells was observed with inverted microscope after 24 h. …”
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Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action by Shaokang Wang, Lingmeng Fu, Yi Wu, Hongmei Xiao, Jing Wang, Guiju Sun

“…The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay. Eca109 cells were then treated with luteolin (0, 40, 160, 240 μM) for 24 h. …”
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Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin by Elena V. Proskurnina, Madina M. Sozarukova, Elizaveta S. Ershova, Ekaterina A. Savinova, Larisa V. Kameneva, Natalia N. Veiko, Maria A. Teplonogova, Vladimir P. Saprykin, Vladimir K. Ivanov, Svetlana V. Kostyuk

“…Cell viability, mitochondrial activity, intracellular reactive oxygen species, NOX4, NRF2, and NF-κB expression, oxidative DNA damage/repair, autophagy, and cell proliferation were studied. We used an MTT assay, fluorescence microscopy, real-time reverse transcription polymerase chain reaction, and flow cytometry. …”
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In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study by Emmanuel Mouafo Tekwu, Kwabena Mante Bosompem, William Kofi Anyan, Regina Appiah-Opong, Kofi Baffour-Awuah Owusu, Mabel Deladem Tettey, Felicia Amanfo Kissi, Alfred Ampomah Appiah, Veronique Penlap Beng, Alexander Kwadwo Nyarko

“…The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC50 > 20 μg/mL appear to be safe. …”
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抗原致敏IL-12基因修饰的树突状细胞诱导抗肾癌免疫的体外研究 by 罗道升, 戴宇平, 张珑涓, 赵继宗, 郑伏甫, 罗彬

“…【方法】采用重组逆转录病毒介导IL-12基因修饰人外周血单个核细胞（PBMC）来源的DC；超声细胞破碎法提取肾癌细胞粗提抗原（Ag）致敏经IL-12转染的DC；ELISA法检测各组DCs和各组CTLs上清中IL-12、IFN-γ因子的分泌水平；流式细胞仪（FACS）分析各组DCs表面CD83、CD86、HLA-DR的表达；MTT法检测DC刺激同源T淋巴细胞增殖的能力及CTL免疫杀伤肾癌细胞的效能；统计学分析比较各组间的差异。…”
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Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration by Andrea Barragán-Cárdenas, Daniel Castellar-Almonacid, Yerly Vargas-Casanova, Claudia Parra-Giraldo, Adriana Umaña-Pérez, Joel López-Meza, Zuly Rivera-Monroy, Javier García-Castañeda

“…Methods: Peptides were obtained by SPPS, characterized by RP-HPLC and ESI-QTOF and its biological activity was evaluated using MTT assays. Evaluation of mechanism associated to the cytotoxic effect were carried out by flow cytometry, RT-qPCR, wound healing, transwell and zymography. …”
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Green synthesis and characterization of CuO/ZnO nanocomposite using Musa acuminata leaf extract for cytotoxic studies on colorectal cancer cells (HCC2998) by Wong Yee Jin, Subramaniam Hemaroopini, Shing Wong Ling, Dhanapal Anto Cordelia Tanislaus Antony, Chan Yu Bin, Aminuzzaman Mohammod, Tey Lai-Hock, Janakiraman Ashok Kumar, Kayarohanam Saminathan, Djearamane Sinouvassane

“…The cytotoxic effect of CuO/ZnO NC toward the colorectal cancer cells (HCC2998) was evaluated by the MTT assay and found to be dose-dependent. The results showed a significant cell mortality at 100 µg·mL−1 CuO/ZnO NC with 45.79% cell death at 24 h. …”
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The Roles of PI3K/AKT/mTOR and MAPK/ERK Signaling Pathways in Human Pheochromocytomas by Juan Du, Anli Tong, Fen Wang, Yunying Cui, Chunyan Li, Yushi Zhang, Zhaoli Yan

“…Cell proliferation was detected by MTT assay. Protein phosphorylation was detected by Western blotting. …”
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Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells by Sukannika Tubtimsri, Tiraniti Chuenbarn, Suwisit Manmuan

“…Methods KON cells were treated with quercetin in order to test the anticancer activity of this compound. The MTT colorimetric assay was used to examine the cell viability of the treated cells in comparison to MRC-5 fibroblast cells. …”
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The Cytotoxicity Assessment of Grapefruit Peel Essential Oil and Its Effect on Frying Stability of Sunflower Oil by Ghania Kaanin-Boudraa, Fatiha Brahmi, Linda Grimaud, Mohamed Lamine Freidja, Ourdia-Nouara Kernou, Daniel Henrion, Samir Hadjal, Khodir Madani, Lila Boulekbache-Makhlouf

“…Methods: Cell viability was evaluated using the MTT-based cytotoxicity assay. Various EO concentrations (0.01-0.5 mg/ml) were added to cultured cells Human Umbilical Vein Endothelial Cells (HUVECs) and Human Stellar Hepatic Cell lines LX-2 (SCC064) and incubated for 24 and 48 h. …”
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Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents by Rozina Ghulam Mustafa, Andleeb Dr Saiqa, Jorge Domínguez, Madiha Jamil, Saira Manzoor, Samna Wazir, Bushra Shaheen, Asma Parveen, Rida Khan, Shaukat Ali, Nazish Mazhar Ali, Fatima Jalal, Sadaf Azad Raja

“…., Escherichia coli, Serratia marcescens, Streptococcus pyogenes, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa (1), Salmonella typhimurium, Shigella flexneri, Enterobacter amnigenus, Serratia odorifera, Pseudomonas aeruginosa (2), Staphylococcus warneri, and Lactobacillus curvatus, via agar well diffusion, crystal violet, MTT, agar disc diffusion, and direct bioautography assays. …”
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CFTR对前脂肪细胞增殖和分化的影响 by 晋晨晨, 杜洁仪, 黄雪莲, 王冠蕾

“…采用地塞米松-异丁基甲基黄嘌呤-胰岛素（DMI）诱导3T3-L1细胞建立前脂肪细胞分化模型，采用免疫印迹（Western blot）技术检测CFTR及分化因子蛋白表达变化；并进一步转染CFTR沉默及过表达病毒，采用免疫印迹，实时荧光定量PCR技术，MTT检测和油红O染色，观察基因干预CFTR后对DMI诱导3T3-L1细胞增殖及分化的影响。采用高脂饮食肥胖小鼠模型，荧光定量PCR技术检测内脏前脂肪细胞增殖分化转录因子和CFTR基因水平表达。…”
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Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALB/c Mice by Thi Thao Do, Thi Phuong Do, Thi Nga Nguyen, Thi Cuc Nguyen, Thi Thu Phuong Vu, Thi Giang An Nguyen

“…The in vitro cytotoxic activity of liposomal L-ASP was evaluated with the MTT assay using cancerous cell lines, and its antitumor effects were examined in Lewis lung carcinoma (LLC) tumorized mice. …”
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