Total Glucosides of Paeony Alleviate Cell Apoptosis and Inflammation by Targeting the Long Noncoding RNA XIST/MicroRNA-124-3p/ITGB1 Axis in Renal Ischemia/Reperfusion Injury by Fang Chen, Yi Hu, Yuetao Xie, Zonghui Zhao, Lin Ma, Zhili Li, Wanlong Tan

“…Then, the viability and apoptosis of renal cells were detected by MTT and flow cytometry assay. Additionally, dual-luciferase reporter assay was used to determine the interactions among XIST, microRNA-124-3p (miR-124-3p), and ITGB1. …”
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慢病毒介导shRNA静默CXCR4对Eca109细胞转移特性的影响

“…【目的】 新近研究显示CXCL12/CXCR4通路在食管癌组织中有较高的表达,本研究探讨应用慢病毒介导的RNA干扰技术,有效静默食管癌细胞株Eca109细胞CXCR4的表达,研究CXCL12/CXCR4通路对食管癌Eca109细胞转移能力的影响65377;【方法】 以人CXCR4 mRNA编码序列作为干扰靶点,构建靶向CXCR4的vshRNA表达质粒,另构建不针对任何已知mRNA的阴性对照vshRNA表达质粒,转染相应组别细胞65377;研究分为控制组65380;阴性对照组及静默组65377;应用实时定量PCR及Western blot检测转染后食管癌细胞CXCR4表达的变化65377;Transwell侵袭小室实验以穿膜细胞的数量评估各组Eca109食管癌细胞的侵袭能力,MTT法观察Eca109各组细胞与Matrigel胶黏附能力的变化,划痕法检测迁移能力改变65377;【结果】 成功构建CXCR4 shRNA慢病毒载体CXCR4-RNAi-LV65377;QPCR及Western blot结果显示静默组Eca109细胞CXCR4 mRNA及蛋白的表达水平较阴性对照组及空白组显著降低(P < 0.05)65377;CXCR4有效静默后,食管癌Eca109细胞黏附65380;侵袭及迁移能力显著下降(P < 0.05)65377;【结论】 慢病毒介导的shRNA能有效地静默食管癌细胞CXCR4基因的表达,阻断CXCL12/CXCR4通路生物学效应,有效抑制食管癌Eca109细胞的转移潜能,提示CXCL12/CXCR4通路在食管鳞癌发展过程中具有重要作用65377;…”
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Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties by Pranay Pramanik, Krishnendu Dakua, Trisha Kar, Ranabir Sahu, Tarun Kumar Dua, Gouranga Nandi, Sangita Dey, Anoop Kumar, Ritu Khanra, Paramita Paul

“…The synthesised CONPs were found to successfully inhibit growth of both Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. Moreover, MTT assay exhibited strong anticancer potential of CONPs against SH-SY5Y neuroblastoma cell-lines with IC50 of 24.14 μg/mL.…”
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Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells by Jing Liu, Liping Yan, Ruilan Niu, Limin Tian, Qi Zhang, Jinxing Quan, Hua Liu, Suhong Wei, Qian Guo

“…Then, HUVEC survival rate was calculated by MTT assay, the levels of NO, endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were detected by colorimetric analysis, and the contents of endothelin-1 (ET-1) were detected by ELISA. …”
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Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4 by Ming Zhang, Chun-Mei Wang, Jing Li, Zhao-Jie Meng, Sheng-Nan Wei, Ji Li, Richard Bucala, Yu-Lin Li, Li Chen

“…The cell viability of HUVECs was determined by MTT assays. Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs. …”
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漆黄素通过调节Apaf-1,ERK和COX-2信号通路诱导人宫颈癌细胞凋亡

“…【方法】 以人宫颈癌Hela细胞为模型,将漆黄素作用于细胞,利用MTT?PI以及Annexin V方法观察细胞的生长和凋亡情况,通过Western blot?RT-PCR以及免疫荧光方法分别考察凋亡相关信号通路的关键蛋白caspase和Apaf-1的表达水平和活性变化,ERK/MAPK信号通路中ERK1/2蛋白的磷酸化水平的变化,以及COX-2蛋白和PGE2的表达水平的变化,并通过使用小分子抑制剂和RNA干扰技术等方法进一步分析上述关键蛋白在漆黄素抗肿瘤细胞生长中的作用?…”
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Palmitic acid-induced cell death: impact of endoplasmic reticulum and oxidative stress, mitigated by L-citrulline by Md. Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura

“…Cell viability was measured with MTT assays. Intracellular reactive oxygen species (ROS) levels in MAC-T cells were assessed using 5-(and-6)-chloromethyl- 2′,7′-dichlorodihydrofluorescein diacetate acetyl ester dye. …”
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Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 by Manjunatha Dadayya, Megha Gowri Thippeswamy, Nagaraju Shivaiah, Thoyajakshi Ramasamudra Siddaraju, Prakash Jayaramaiah, Sowmya Hirakannavar Veeranna, Thippeswamy Basaiah, Shridhar N Mathad, Ravikumar Hemagiri Gowda, Sachin Naik, Abdulaziz Abdullah AI Kheraif, Sajith Vellappally

“…Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs.…”
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Design of the Multi-Bioactive Graphene-Oxide/Gelatin/Alginate Scaffolds as Dual ECM-Mimetic and Specific Wound Healing Phase-Target Therapeutic Concept for Advanced Wound Healing by Marko Demenj, Martina Žabčić, Marija Vukomanović, Tatjana Ilić-Tomić, Dušan Milivojević, Simonida Tomić, Dubravka Živanović, Marija M. Babić Radić

“…Biocompatibility of the scaffolds were evaluated in vitro by MTT test on fibroblasts (MRC5 cells) and in vivo using <i>Caenorhabditis elegans</i> assay. …”
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Anti-apoptosis Effect of Gualou Guizhi Granule on Neurons in Ischemic Stroke Rats and Primary Hippocampal Neurons by Yu LIN, Wei XU, Yuqin ZHANG, Huang LI, Wen XU

“…Objective:To investigate the anti-apoptosis effect of Gualou Guizhi granule(GLGZG)on neurons in ischemic stroke rats and primary hippocampal neurons, and then explore the involved protective mechanism of GLGZG, thus providing a theoretical basis for the treatment of stroke.Methods:Glutamate-induced primary hippocampal neurons excitotoxicity injury model and focal cerebral ischemic-reperfusion injury model were established. MTT and LDH assay were used to detect the effect of GLGZG on primary hippocampal neurons and LDH activity. …”
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上调microRNA-205表达对HeLa细胞生物学特性的影响

“…【目的】 探讨上调microRNA-205(miR-205)表达对HeLa细胞生物学特性的影响65377;【方法】 实验分4组:miR-205上调组,阴性对照组,空白转染组和空白对照组65377;每种检测重复3次65377;利用脂质体siPORT NeoFX Transfection Agent,向miR-205上调组细胞转染Pre-miR-hsa-miR-205 miRNA Precursor,阴性对照组细胞转染Cy3 dye labeled PremiR Negative Control65377;荧光显微镜下评估转染情况,实时定量PCR方法检测miR-205表达,四甲基偶氮唑蓝(MTT)法和平板克隆形成实验分别检测细胞生长和增殖能力,流式细胞术检测细胞凋亡率和细胞周期分布,Transwell小室法检测细胞迁移能力65377;【结果】 实时定量PCR结果显示,miR-205上调组miR-205的表达增加3061.5倍65377;与空白对照组相比,72 h miR-205上调组与阴性对照组活细胞数分别为(126 ± 41)%,(126 ± 39)%;两组克隆形成率分别为(79 ± 11)%,(63 ± 10)%;凋亡率分别为(4.6 ± 2.1)%,(4.1 ± 2.4)%;S期细胞比例分别为(19.2 ± 3.6)%,(23.4 ± 3.0)%;200倍镜视野下迁移细胞数分别为63 ± 17和68 ± 1665377;与对照组比,miR-205上调组细胞增殖能力增强但生长曲线不受影响,S期细胞比例降低,而凋亡率不变,细胞迁移能力无明显改变65377; 【结论】 上调miR-205表达后,HeLa细胞增殖能力及细胞周期改变,但生长曲线65380;凋亡率和迁移能力无变化65377;…”
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Physical, chemical and biological properties of MTA Angelus and novel AGM MTA: an in vitro analysis by Sara Nashibi, Parisa Amdjadi, SeyedehSana Ahmadi, Sara Hekmatian, Maryam Torshabi

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大蒜素对神经母细胞瘤生长的抑制作用

“…【目的】 探讨大蒜素对神经母细胞瘤生长的影响及相关机制65377;【方法】 MTT法测定不同浓度大蒜素对神经母细胞瘤细胞生长的影响;建立裸鼠人神经母细胞瘤皮下移植瘤模型24只(n = 8),随机分为对照组65380;大蒜素Ⅰ组(1 mg8226;kg-18226;d-1)和大蒜素Ⅱ组(10 mg8226;kg-18226;d-1),观察其对肿瘤生长的影响,荧光定量PCR法测定大蒜素对裸鼠皮下移植瘤VEGF mRNA表达的影响;ELISA法测定裸鼠血浆VEGF浓度;免疫组化法观察肿瘤微血管密度65377;【结果】 不同浓度的大蒜素均能抑制神经母细胞瘤细胞的生长,高浓度的大蒜素能直接杀死肿瘤细胞;大剂量和小剂量大蒜素对裸鼠神经母细胞瘤皮下移植瘤均有抑制作用65377;对照组65380;大蒜素Ⅰ组和Ⅱ组的肿瘤体积为(2143 ± 127) mm365380;(1041 ± 89) mm3和(904 ± 65) mm3 (P < 0.05);大蒜素能下调神经母细胞瘤VEGF mRNA转录,对照组65380;大蒜素Ⅰ组和Ⅱ组VEGF mRNA表达量分别为(8.4 ± 0.8)65380;(6.1 ± 0.7)和(5.1 ± 0.5);大蒜素能够抑制肿瘤细胞VEGF的表达,对照组65380;大蒜素Ⅰ组和Ⅱ组裸鼠血VEGF浓度分别为(78 ± 10)65380;(45 ± 8)和(35 ± 7) pg/mL;大蒜素可以减少神经母细胞瘤的微血管密度65377;【结论】 大蒜素在体内和体外均可抑制神经母细胞瘤细胞的生长,其机制与大蒜素抑制肿瘤细胞增殖和下调神经母细胞瘤VEGF mRNA的转录和蛋白表达有关65377;…”
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PTEN基因对子宫内膜癌细胞PI3K/AKT通路的影响

“…【方法】利用慢病毒载体系统, 构建人PTEN基因RNA干扰慢病毒载体和PTEN基因过表达慢病毒载体,分别转染PTEN野生型的HEC-1A细胞和PTEN突变型的Ishikawa细胞, 建立PTEN 基因敲减及过表达的细胞模型，Western Blot方法检测PTEN基因敲减或过表达前后PTEN蛋白的表达和AKT通路的活化情况, MTT法检测慢病毒转染前后细胞增殖的变化,流式细胞术检测慢病毒转染前后细胞周期及细胞凋亡率的变化。…”
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姜酚肟对谷氨酸诱导PC12细胞损伤的保护作用 by 杜瑞, 王辉, 黄雪松, 郭国庆, 沈伟哉

“…【方法】体外培养PC12细胞，建立谷氨酸诱导PC12细胞损伤的模型；采用MTT法检测细胞活力，流式细胞术和Hoechst 33258 DNA 染色法检测细胞凋亡。【结果】经5 mmol/L的谷氨酸处理24 h后，PC12细胞活力比对照组降低，仅为对照组的58.3﹪。…”
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调控microRNA-99b表达对宫颈癌SiHa细胞增殖和凋亡的作用

“…实时定量PCR方法检测miR-99b表达，四甲基偶氮唑蓝（MTT）法检测细胞增殖能力，流式细胞术检测细胞凋亡率，Western blot检测靶基因细胞分裂周期25A（CDC25A）蛋白表达。…”
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Isoforsythiaside confers neuroprotection against Alzheimer’s disease by attenuating ferroptosis and neuroinflammation in vivo and in vitro by Chunyue Wang, Hongbo Jiang, Honghan Liu, Shanshan Chen, Hangyu Guo, Shuoshuo Ma, Weiwei Han, Yu Li, Di Wang

“…Additionally, IFY upregulated the expression levels of GPX4, FTH, FTL, p-GSK-3β, Nrf2, and NQO1, and downregulated the expression of TFR1, DMT1, p-Fyn, GFAP, p-IKKα+β, p-IκBα, p-NF-κB, and pro-inflammatory factors in the brains of APP/PS1 mice and erastin-damaged HT22 cells. …”
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Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses Ethanol/Indomethacin-Induced Chronic Gastric Injury in Rats by Vanessa Mba Matah Marte, Gilbert Ateufack, Marius Mbiantcha, Albert Donatien Atsamo, Carine Flore Adjouzem, Stéphanie Flore Djuichou Nguemnang, Eric Gonzal Tsafack, William Yousseu Nana, Yacine Karelle Madjo Kouam, Elvira Ngoufack Azanze

“…The healing properties of gastric ulcers (chronic ulcer model induced by ethanol and indomethacin) were evaluated in vivo in adult male rats, while the mechanisms of action were evaluated in vitro by anti-inflammatory assay (protein denaturation, cyclooxygenase, and lipoxygenase assays) and immunomodulatory assay (ROS production (using technical chemiluminescence), cytokine (TNF-α, IL-1β, IL-6) production (using ELISA), proliferation of T cells (using liquid scintillation counter), and cytotoxicity (using MTT assay)). The methanolic extract of Distemonanthus benthamianus inhibited protein denaturation (75.63%) and the activities of cyclooxygenase (78.92%) and 5-lipoxygenase (81.54%). …”
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Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers by Marcos Coelho Santiago, Gustavo Henrique de Oliveira Salles, Gustavo Gomes de Lima, Laudimar Alves de Oliveira, Loise Pedrosa Salles

“…Background: The present study aimed to compare the viability, proliferation, and mineralization potential of human dental pulp cells (hDPCs) and osteoblasts cell line (Saos-2) after exposure to AH Plus® Bioceramic (AHP-B), Bio-C® Sealer (BIO-C), NeoMTA Plus® (NEOMTA-P), and MTA-FILLAPEX® endodontic sealers (MTA-F). …”
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TFE3 Regulates the Function of the Autophagy-Lysosome Pathway to Drive the Invasion and Metastasis of Papillary Thyroid Carcinoma by Hongsheng Lu, Chumeng Zhu, Yanyun Ruan, Lilong Fan, Kena Wei, Zhaohui Yang, Qi Chen

“…After inducing TFE3 overexpression by plasmid or TFE3 downregulation by small interfering RNA (siRNA) transfection, MTT, wound healing, and cell migration and invasion assays were used to verify the effects on invasion, migration, and the levels of autophagy-lysosome system-related proteins such as P62/SQSTM1, LC3, CTSL, and CTSB. …”
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