MicroRNA-32-5p promotes the proliferation and metastasis of gastric cancer cells by Chao Sun, Lai-gang Huang, Bing Leng, Yanting Guo, Chen Chen, Ruijie Lv, Yan Dong, Tian-tian Gao, De-qing Sun

“…Micro-RNAs were reported to participate in the progression of GC. Clonal formation, MTT, caspase-3 activity, sperm DNA fragmentation, flow cytometry assay, cell adhesion, transwell assays were performed to detect the functions of miR-32-5p or anti-miR-32-5p on the growth and metastasis of GC cells. …”
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Socialinių paslaugų srities darbuotojų profesinis perdegimas ir jį lemiantys veiksniai individualios priežiūros darbuotojų lygmeniu by Andrius Šmitas, Edita Auškalnytė

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丙烯醛体内外诱导神经元铁死亡的初步研究 by 彭伟嘉, 朱泽宇, 杨扬, 侯加卫, 皮荣标

“…方法体外：给予小鼠海马神经元细胞系HT22细胞丙烯醛进行造模，采用铁死亡抑制剂铁抑素-1（Fer-1）与去铁胺（DFO）进行保护。MTT法检测细胞存活率；自由基探针二氢乙啶（DHE）与亚铁探针FerroOrange检测细胞内自由基与亚铁离子含量；透射电镜观察正常与模型组的线粒体超微形态与结构；Western blot法检测铁死亡相关蛋白表达的情况。…”
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Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways by Liang Zhu, Yanchi Bi, Ting Liang, Po Zhang, Xiao Xiao, Tengbo Yu

“…GK's anti-inflammatory and antioxidative effects were comprehensively assessed via MTT assay, western blot, cell immunofluorescence, ELISA, and transcriptome sequencing. …”
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Investigation on the strength of expanded clay aggregate concrete specimens after thermal exposure by Danguolė Plungytė, Ričardas Plungė

Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…Cell growth estimations were made through MTT assay. Phase contrast microscopy was used for the analysis of the viability of multiple myeloma cells. …”
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氧化苏木素诱导人乳癌MCF-7细胞凋亡及其作用机制

“…【目的】 探讨氧化苏木素对人乳癌MCF-7细胞的凋亡作用及其相关作用机制 【方法】 MTT实验检测氧化苏木素对MCF-7细胞和正常乳腺上皮细胞MCF 10A的细胞毒作用;Annexin V/PI双染,流式细胞仪检测细胞凋亡的发生;荧光染料DiOC6染色,流式细胞仪检测细胞内线粒体膜电位的变化;Western blot实验检测细胞浆细胞色素C及细胞内caspase-9和survivin蛋白的变化 【结果】 氧化苏木素对人乳癌MCF-7细胞具有高效的细胞毒作用,而对正常乳腺上皮MCF 10A细胞毒性较弱,IC50值分别为(7.15 ± 0.43)μmol/L 和(33.56 ± 2.87) μmol/L;07.51530 μmol/L的氧化苏木素处理MCF-7细胞48 h后,细胞的凋亡率从(3.37 ± 0.66)%依次升高到(16.8 ± 1.27)%(31.31 ± 4.22)%(51.23 ± 5.55)%;DiOC6染色流式细胞仪检测结果显示氧化苏木素可以剂量依赖性地降低线粒体膜电位;Western blot结果显示氧化苏木素诱发了线粒体内细胞色素C的释放和细胞内caspase-9蛋白的剪切激活,同时氧化苏木素也可剂量依赖性地降低survivin蛋白的表达 【结论】 氧化苏木素可通过线粒体通路诱导MCF-7细胞凋亡,下调survivin蛋白可能是其诱导细胞凋亡的主要机制…”
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大黄蛰虫丸对大鼠肝星状细胞活化增殖的影响

“…【目的】 观察大黄蛰虫丸对经旁分泌和自分泌途径激活大鼠肝星状细胞活化增殖情况的影响65377;【方法】 采用Nycodenz 分离液非连续密度梯度离心法同步分离急性CCl4 损伤模型大鼠和正常大鼠肝的枯否细胞(KC)和星状细胞(HSC)65377;在制备正常鼠血清和DHZCW药物血清的基础上,将它们分别加入KC和HSC培养板内作用后制备成正常鼠KC条件培养液(NKCCM)65380;损伤鼠KC条件培养液(KCCM)以及正常鼠HSC条件培养液(HSCCM)65377;将上述条件培养液分别干预HSC 24 h65380;48 h和72 h,然后采用MTT法和3H-TdR掺入法分别检测比较各组HSC细胞活化增殖情况65377; 【结果】 损伤鼠不含药组和正常KC对照组比较,HSC活化增殖情况显著性增高(P < 0.01),损伤鼠含药组HSC活化增殖则显著降低(P < 0.01,P < 0.05),且其抑制效应随着含药血清浓度的升高逐渐增强;而正常鼠HSC不含药组和正常鼠HSC含药组各组间比较均无显著性差异(均P > 0.05)65377; 【结论】 DHZCW对经旁分泌途径激活大鼠HSC活化增殖有明显的抑制作用,且作用效应与血清药物浓度间存在剂量依赖关系,但对经自分泌途径激活大鼠HSC活化增殖未见明显抑制作用65377;…”
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Tissue-friendly dentin treatments as a potential element in revascularization protocol (ex-vivo study) by Hala Fayek Khalil, Nadia Ezz El Din Metwalli, Sara Magdy, Mohamed Shamel

“…Cytotoxicity was assessed using the MTT assay, with optical density measured at the specified times. …”
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Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis by Zhi-Bo Zhang, Zheng Shi, Lan-Fang Yang, Hong-Bin Gao

“…SMMC7721 cell proliferation, cycle, apoptosis, and invasiveness were detected by MTT, flow cytometry, Annexin V-FITC/PI, and invasion assay, respectively. …”
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Design and synthesis of antiproliferative 2-oxoindolin-3-ylidenes incorporating urea function with potential VEGFR-2 inhibitory properties by Dalia R. Aboshouk, M. Adel Youssef, Siva S. Panda, Benson M. Kariuki, Mohamed S. Bekheit, Ahmed R. Hamed, Walid Fayad, Ahmed A. F. Soliman, Adel S. Girgis

“…Promising antiproliferation properties (MTT assay) were observed for most of the synthesized agents against HCT116 (colon), MCF7 (breast) and PaCa2 (pancreatic) cancer cell lines relative to sunitinib. …”
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Essential oils from Amorpha fruticosa against hepatocellular carcinoma based on network pharmacology by Yixian Liu, Xiaojun Zhang, Jiacong Hao, Ying Zhao, Min Zou, Huiping Chen, Jintao Zhang

“…The inhibitory effect of the EOs on HCC was evaluated by MTT assay. The detected components of AFLEO and AFFEO were performed ADME screening to examine their drug-likeness. …”
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Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 by Shida Chen, Defen Shen, Nicholas A. Popp, Alexander J. Ogilvy, Jingsheng Tuo, Mones Abu-Asab, Ting Xie, Chi-Chao Chan

“…Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of Il-6 and Il-17a was detected by quantitative RT-PCR. …”
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槲皮素诱导耐甲氨蝶呤骨肉瘤细胞系U2-OS/MTX300凋亡

“…【目的】 研究槲皮素对耐甲氨蝶呤骨肉瘤细胞株U2-OS/MTX300的增殖抑制和诱导凋亡作用及相关机制【方法】 以不同浓度槲皮素及甲氨蝶呤处理U2-OS及U2-OS/MTX300采用四甲基偶氮唑蓝(MTT)法及平板克隆检测细胞增殖,流式细胞仪检测细胞周期,Hochest 33258 染色及流式细胞仪检测凋亡,Western blot法检测凋亡相关蛋白cleaved-PARP,Bax,Bak,Bcl-2及Bcl-XL表达【结果】 槲皮素可明显抑制耐甲氨蝶呤骨肉瘤细胞U2-OS/MTX300的增殖,72 h IC50值为(22 ± 4)μmol/L102550μmol/L可显著抑制U2-OS/MTX300克隆形成数量,且随剂量增加抑制作用明显增强槲皮素与甲氨蝶呤之间不存在交叉耐药性槲皮素能诱导U2-OS/MTX300细胞S期阻滞,并可诱导胞内产生典型凋亡小体,流式细胞仪可检测到明显凋亡其作用机制是通过上调Bax,Bak及下调Bcl-2表达,促进cytochrome C释放及诱发PARP等重要胞内蛋白断裂来实现【结论】 槲皮素可显著抑制耐甲氨蝶呤骨肉瘤细胞系U2-OS/MTX300细胞增殖并诱导其凋亡,线粒体途径可能是其诱导凋亡的重要机制…”
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Synthesizing hybrid copper phosphate (Cu3(PO4)2) nanoflowers using Cu+2 and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation... by Cagri Caglar Sinmez, Fatih Doğan Koca

“…The cytotoxicity of Cu@SSS hNFs on the lung carcinoma (A549) cell line was shown to be concentration-dependent by the MTT test assay (IC50: 56.4 µg/ml). Conclusion As a result, Cu-based hNFs synthesized by using an organic waste (SSS) might be improved for environmental and biomedical applications.…”
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Gene Therapy with HSV1-sr39TK/GCV Exhibits a Stronger Therapeutic Efficacy Than HSV1-TK/GCV in Rat C6 Glioma Cells by Lei-qing Li, Fang Shen, Xiao-yan Xu, Hong Zhang, Xiao-feng Yang, Wei-guo Liu

“…Then the in vivo sensitivity of these transfected C6-TK and C6-sr39TK cells to GCV was determined by 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric assay and Hoechst-propidium iodide (PI) staining. …”
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Encapsulated LyeTx III Peptide: Cytotoxic Agent Isolated from <i>Lycosa erythrognatha</i> Spider Venom by Daniel Moreira dos Santos, Livia Ramos Santiago, Nayara Araújo dos Santos, Wanderson Romão, Jarbas Magalhães Resende, Maria Elena de Lima, Márcia Helena Borges, Rosy Iara Maciel de Azambuja Ribeiro

“…Significantly, when liposome-encapsulated, LyeTx III demonstrates selective activity against tumor cells in culture. Our MTT results showed that the cytotoxicity of the peptide increased against HN13 cells when administered as liposomes, with their viability in HN13 cells alone being 98%, compared to 38% in liposome-encapsulated form. …”
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Increased Anti-Proliferation Performance of NanoChitosan-Moringa Seeds Extract and Co-Treatment with Doxorubicin in Liver Cancer Cells by Hendra Susanto, Moch Sholeh, Agustina Tri Endharti, Nik Ahmad Nizam Nik Malek, Sa’diyatul Rizqie Amaliyah Firdaus, Adeodatus Yuda Handaya, Ahmad Taufiq

“…The IC50 dose was investigated using the MTT assay. Then, the IC50 dose was confirmed in more detail through immunofluorescence, betatrophin gene, several genes in the Wnt-βcatenin-CyclinD1 proliferation pathway, and the addition of the apoptotic effector Caspase-3 using RT-qPCR analysis. …”
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Atsakingo vartojimo raiška Lietuvos akademinio jaunimo tarpe maisto produktų vartojimo atveju by Rasa Bartkutė, Vitalija Skėruvienė, Aušra Arminienė, Valdemaras Makutėnas

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Lipid-Rich Extract from Mexican Avocado Seed (Persea americana var. drymifolia) Reduces Staphylococcus aureus Internalization and Regulates Innate Immune Response in Bovine Mammary... by Marisol Báez-Magaña, Alejandra Ochoa-Zarzosa, Nayeli Alva-Murillo, Rafael Salgado-Garciglia, Joel Edmundo López-Meza

“…The effects of LEAS (1-500 ng/ml) on the S. aureus growth and bMEC viability were assessed by turbidimetry and MTT assays, respectively. LEAS did not show neither antimicrobial nor cytotoxic effects. …”
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