Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model by Radka Pecková, Karel Doležal, Bohumil Sak, Dana Květoňová, Martin Kváč, Ivan Petřík, Wisnu Nurcahyo, Ivona Foitová

“…The cytotoxicity of the extracts was determined by the MTT Cell Viability Assay. Results We recorded a statistically-significant decrease in giardia trophozoites in two extracts: the methanolic extract of A. fagifolium (Kruskal-Wallis: p = 0.013) and the aqueous extract of P. betle (Kruskal-Wallis: p = 0.002). …”
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Toxic effects of polystyrene nanoplastics on MDA-MB-231 breast cancer and HFF-2 normal fibroblast cells: viability, cell death, cell cycle and antioxidant enzyme activity by Hanie Sadeghinia, Parichehr Hanachi, Reihaneh Ramezani, Samaneh karbalaei

“…Antibacterial effects were assessed using the agar dilution method, while the MTT assay determined cytotoxicity in MDA-MB-231 and HFF-2 cells. …”
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Cytotoxicity and Exposure Assessment of PM2.5 in a Residential Home during COVID-19 Lockdown by Ummi Sabariah Mahamud, Armania Nurdin, Eliani Ezani

“…PM2.5 samples were then extracted using methanol. MTT assay determined the cytotoxic activity of extracted indoor and outdoor PM2.5 treated at different concentrations (25–200 µg mL−1) on human lung cells (MRC-5) at a 24-hour incubation period. …”
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Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis by Wang Fang, Zhang Yang, Pang Rui, Shi Shaohong, Wang Ran

“…Epithelial to mesenchymal transition (EMT) and stemness-related protein markers were monitored by real-time PCR analysis and immunoblotting. …”
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Aβ 不同片段肽疫苗免疫鼠后血清对 Aβ42诱导细胞毒性作用的影响

“…【方法】①用间接ELISA法测定Aβ42和K7Aβ36-42疫苗接种鼠后的血清中抗体滴度;②将不同浓度的Aβ42和K7Aβ36-42加入培养有PC12细胞的96孔培养板中,检测其对PC12细胞的毒性作用;③用不同浓度的Aβ42及K7Aβ36-42疫苗免疫鼠血清加入含Aβ42的培养基中,与PC12一起孵育培养,7d后用MTT法和酶标仪测定存活细胞的A值。【结果】①Aβ42和K7Aβ36-42疫苗接种后血清的平均抗体滴度分别为1∶6800和1∶4600;②10mg/L和20mg/LAβ42组细胞的存活率与空白组相比差异有显著性(P<0.01);③各浓度K7Aβ36-42组与空白组相比差异均无显著性(P>0.05)。…”
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Comparative Cytotoxic Activity of Wild Harvested Stems and In Vitro-Raised Protocorms of Dendrobium chryseum Rolfe in Human Cervical Carcinoma and Glioblastoma Cell Lines by Bijaya Pant, Pusp Raj Joshi, Sabitri Maharjan, Laxmi Sen Thakuri, Shreeti Pradhan, Sujit Shah, Sven H. Wagner, Basant Pant

“…The comparative cytotoxic activities of the wild and in vitro-raised protocorms were evaluated in human cervical carcinoma (HeLa) and human glioblastoma (U251) cell lines by MTT assay. In in vivo and in vitro, the methanol extracts of D. chryseum showed significant cytotoxic activities. …”
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Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction via mTOR/p70S6K/4E-BP1 Pathway by Chuan Lv, Can Wu, Yue-hong Zhou, Ying Shao, Guan Wang, Qiu-yue Wang

“…The effect of LA on high glucose-induced cell proliferation, fibronectin (FN), and collagen type I (collagen-I) expression and its mechanisms were examined in cultured rat mesangial cells by methylthiazol tetrazolium (MTT) assay, flow cytometry, ELISA assay, and western blot, respectively. …”
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Potential of Co(II) and Cd(II) chelates of N′-(3-fluorobenzoyl)benzo[d]thiazole-2-carbohydrazide: Structural aspects, binding interactions, biological and molecular docking studies... by K. Srilaxmi, B. Sireesha

“…Cytotoxicity of the compounds was performed for Hela and MCF cell lines by MTT assay. Molecular docking studies carried out using Auto Dock 4.2 program revealed HL and Co(II)-MFBBTCH docking at the minor groove, and Cd(II)-MFBBTCH was docked at the major groove with hydrogen bonding.…”
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靶向SMPD1基因的RNA干扰对人成纤维细胞凋亡的保护作用 by 高军, 周灿权, 张仁礼, 马芸

“…【方法】设计合成三对靶向SMPD1基因的小干扰RNA作为实验组,同时设立阴性对照组和脂质体组（lipofectamine 2000）,瞬时转染原代培养的HFF细胞,采用荧光定量RT-PCR法及Western blot测定SMPD1表达抑制情况,并用化疗药物丝裂霉素诱导细胞凋亡,MTT法检测siRNA转染对细胞的保护作用。【结果】小干扰RNA1,2,3对SMPD1基因mRNA的抑制效率分别为96.3%,39%,63.2%,以siRNA1最高,最佳浓度为50nmol/L,最佳作用时间为48h。…”
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Socialinio darbuotojo kompetencijos atliekant įvairius vaidmenis dirbant su psichikos negalią turinčiais asmenimis by Tomas Virbalis, Inga Arbataitienė

مقایسه‌ی روش‌های درمانی رادیوتراپی و پلاسمای سرد در مهار رشد سلول‌های سرطانی by سارا مومنی, احمد شانئی

“…این مطالعه با هدف بررسی مقایسه‌ی روش‌های درمانی رادیوتراپی و پلاسمای سرد بر رده‌های سلولی سرطانی ملانوما و سالم فیبروبلاست انجام شد.روش‌ها: سلول‌های سرطانی ملانوما (DFW) و سالم فیبروبلاست (HFF) تحت رادیوتراپی با دزهای 1، 2 و 4 گری و تابش پلاسمای سرد در زمان‌های مختلف 15، 30، 60 و 90 ثانیه قرار گرفتند. سپس نتایج آزمون MTT  با یکدیگر مقایسه شد. علاوه بر این، میزان آپپتوز نیز در هر گروه با استفاده از آزمون فلوسایتومتری مورد بررسی قرار گرفت.یافته‌ها: در حالیکه درصد بقا در سلول‌های ملانوما درمان شده با رادیوتراپی حتی در دزهای بالا، تغییرات چشمگیری نسبت به گروه شاهد نداشت، در سلول‌های درمان شده با پلاسمای سرد به 36 درصد رسید. …”
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Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer by Asmaa M. Elfiky, May M. Eid, May El-Manawaty, Zeinab A. Elshahid, Elham Mohamed Youssef, Khaled Mahmoud

“…UV/visible and FTIR spectroscopy, Zeta potential and MTT were used to confirm the allocation of the miR-497 on SPION@Ag@Cs and its cytotoxicity against CRC cell lines. …”
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Influence of Mesalazine on Ferroptosis-Related Gene Expression in In Vitro Colorectal Cancer Culture by Joanna Słoka, Barbara Strzałka-Mrozik, Sebastian Kubica, Ilona Nowak, Celina Kruszniewska-Rajs

“…Cytotoxicity was assessed using the MTT assay, while ferroptosis-related gene expression analysis was performed using oligonucleotide microarrays, with RT-qPCR used for validation. …”
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Antitumor Effect of Demethylzeylasteral (T-96) on Triple-Negative Breast Cancer via LSD1-Mediate Epigenetic Mechanisms by Zhengjie Shen, Yongjuan Gu, Ruiyang Jiang, Heya Qian, Siyuan Li, Lixian Xu, Wenzhe Gu, Yun Zuo

“…Experimental approach. MTT assay, Live and Dead cell assay, and TUNEL were used to observe the antitumor effect of breast cancer cells treated with T-96. siRNA of LSD1, Co-IP, and molecular docking were used to explore the direct target and mechanism of T-96. …”
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Oleuropein in olive leaf, branch, and stem extracts: stability and biological activity in human cervical carcinoma and melanoma cells by Benčić Đani, Barbarić Monika, Mornar Ana, Klarić Daniela Amidžić, Brozovic Anamaria, Dabelić Sanja, Fadljević Mihaela, Marković Ana Karković

“…The biological activity of extracts was determined on cervical cancer (HeLa), melanoma (A375), metastatic melanoma (A375M) tumor cell lines, and on spontaneously immortalized cell line of keratinocytes (HaCaT), using the MTT assay. The data show that all extracts had a similar dose-dependent effect on cell viability in HeLa cells, while the effect of LE on melanoma A375 and A375M, and HaCaT cells was cell-line dependent.…”
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Agro-morphological characterization and assessment of metabolic profiling and anticancer activities in various tribulus (Tribulus terrestris L.) populations by Yasamin Dabaghkar, Ghasem Eghlima, Hossein Behboudi, Mohammadtaghi Ebrahimi, Mansour Ghorbanpour

“…The minimum (0.65 mg/g DW) and maximum (7.49 mg/g DW) diosgenin content was observed in KHA and PAN populations, respectively. The results of the MTT assay demonstrated the significant anti-proliferative activity of the T. terrestris extracts against the PC3 cancer cell line. …”
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London Rocket (<i>Sisymbrium irio</i> L.) as Healthy Green: Bioactive Compounds and Bioactivity of Plants Grown in Wild and Controlled Environments by Tarik Chileh-Chelh, Tatiana Pagan Loeiro da Cunha-Chiamolera, Miguel Urrestarazu, Mohamed Ezzaitouni, Rosalía López-Ruiz, Cinthia Nájera, Miguel Ángel Rincón-Cervera, José Luis Guil-Guerrero

“…Phenolic content was higher in wild plants than in the GCE ones, with apigetrin as the primary phenolic compound. The MTT assay showed that ethanol extracts from wild plants weakly inhibited HT-29 cell growth, with a GI₅₀ of 210–380 µg mL⁻¹ after 72 h of cells exposure to plant extracts. …”
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Pan-cancer analysis of the immunological and oncogenic roles of ATAD2 with verification in papillary thyroid carcinoma by Zhecheng Li, Yaxin Zhao, Peng Huang, Zhaoyi Wu, Dengjie Ouyang, Adolphus Osei Nyarko, Lei Ai, Zhejia Zhang, Shi Chang

“…Additionally, the expression and oncogenic functions of ATAD2 were verified in papillary thyroid carcinoma (PTC) through MTT, wound-healing, transwell, and flow cytometry assays. …”
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Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells by Xinfu LIN, Ling SHI, Hengzhao ZHU, Sujuan CHEN, Xuehua ZHENG, Yiman WU

“…The cell proliferation was observed by MTT assay, the expression of TLR4, MyD88 and NF-κB mRNA were detected by RT-PCR, and their protein expressions were detected by Western Blot.Results:Compared with the blank control group, the proliferation of PC12 cells in the model group was decreased (<italic>P</italic>&lt;0.01), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were increased significantly (<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05). …”
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Polifenolinių junginių kiekio ir antioksidacinio aktyvumo palyginimas pipirmėtės (Mentha piperita L.) ir katžolės (Nepeta faassenii L.) ekstraktuose by Jolanta Jurkevičiūtė, Ingrida Radveikienė, Nijolė Ružienė