STUDENTŲ POŽIŪRIS Į SOCIALIAI ATSAKINGO VERSLO SIEKIUS LIETUVOJE by Sandra Žukauskienė, Egidijus Žukauskas

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MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. Also, related protein expressions on Wnt/β-catenin signaling pathway were analyzed by Western blot method. …”
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Dantų tiesinimo kapas nešiojančių odontologijos klinikos pacientų dantų priežiūros bei mitybos įpročių sąsajos su burnos sveikata by Brigita Kasmauskytė, Evelina Daugėlienė

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The Synergistic Effects of Celastrol in combination with Tamoxifen on Apoptosis and Autophagy in MCF-7 Cells by Lijun Wang, Luqun Tang, Chengyun Yao, Chunyan Liu, Yongqian Shu

“…Our study is aimed at exploring the interactive antitumour effects of celastrol combined with tamoxifen and the potential underlying anticancer mechanisms in MCF-7 cells. The results from MTT assays, isobolographic analyses, and clonogenic cell survival assays revealed that a combination of celastrol and tamoxifen exerted synergistic cytotoxic effects in MCF-7 cells. …”
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In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions by Fatmagul Yur, Semiha Dede, Sedat Cetin, Mehmet Taspinar, Ayse Usta

“…The control group determined glucose's IC50 value based on the viability tests executed on MTT cells. Cells were seeded in plates as each would have 2x106 cells. …”
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Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis by Hongyan Chen, Qing Miao, Miao Geng, Jing Liu, Yazhuo Hu, Lei Tian, Jingkun Pan, Yi Yang

“…The antiproliferation of rutin in human neuroblastoma cells LAN-5 were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Chemotaxis of LAN-5 cells was assessed using transwell migration chambers and scratch wound migration assay. …”
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SOX7 is involved in polyphyllin D-induced G0/G1 cell cycle arrest through down-regulation of cyclin D1 by Zheng Bin, Wang Gang, Gao Wenbo, Wu Qiquan, Zhu Weizhi, Weng Guobin

“…PC-3 cells were cultured in complete medium containing PD for 12, 24, and 48 h. MTT assay was used to investigate the cytotoxic effects of PD. …”
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MEKANISME KO-KEMOTERAPI EKSTRAK ROSELLA (Hibiscus sabdariffa. L) DENGAN DOXORUBICIN PADA SEL KANKER SERVIKS (HeLa) SECARA IN VITRO by Intan Daud, Laela Hayu Nurani, Moch. Saiful Bachri, Citra Ariani Edityaningrum, Muhammad Ma’ruf

“…Metode uji sitotoksik, antiproliferasi, apoptosis, dan kombinasi dengan metode MTT menggunakan sel HeLa. Hasil dari uji sitotoksik menunjukkan IC50 Ekstrak Etanol Rosella sebesar 32,3 ± 2,15 µg/mL dan IC50 Doxorubicin sebesar 2,68 ± 0,09 µg/mL. …”
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15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells by Carlos Antônio Trindade-da-Silva, Carolina Fernandes Reis, Lara Vecchi, Marcelo Henrique Napimoga, Marcelo Sperandio, Bruna França Matias Colombo, Patrícia Terra Alves, Laura Sterian Ward, Carlos Ueira-Vieira, Luiz Ricardo Goulart

“…In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. …”
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Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochem... by Elumalai Devan, Hemavathi Maduraiveeran, Kavitha Raja, Arulvasu Chinnasamy, Govindan Sivalingam, Sathya Balaji, Vinoth Neelakandan, Sharon Agnita, Murugan Rajaram

“…Upon 24 h of gestation, the frozen cells began to shrink and seem round in shape. Using the MTT assay, the mucus crude extract was evaluated for its anticancer properties against the human colon cancer cell line (HT 29). …”
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SPAG5低表达与早期卵巢高级别浆液性癌患者预后关系 by 袁林静, 黄舒婷, 黄佳明, 邓松清, 姚书忠

“…【方法】体外实验中通过瞬时敲降卵巢腺癌细胞株中SPAG5，紫杉醇处理细胞后，MTT法检测细胞 活力及绘制细胞生长曲线，研究SPAG5对细胞生长增殖及紫醇敏感性的影响；然后通过免疫组织化学染色，研究110例患 者组织中SPAG5表达水平与患者预后的关系。…”
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顺铂耐药卵巢癌细胞化疗敏感性实验

“…【方法】 培养基中不含药物的处理组为对照组,加入不同浓度化疗药物的处理组为实验组。在相差显微镜下观察细胞形态变化;用MTT法测定单药应用。双药联合使用对两种细胞生长的影响;流式细胞仪分析细胞周期和凋亡率的变化。【结果】 SKOV3∕DDP细胞耐药指数为7.10 ± 2.19。…”
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BŪSIMŲJŲ PEDAGOGŲ POŽIŪRIS Į SAVANORIŠKOS VEIKLOS BEI „MINKŠTŲJŲ“ GEBĖJIMŲ UGDYMO(SI) SĄSAJAS: LIETUVOS IR LATVIJOS AUKŠTŲJŲ MOKYKLŲ ATVEJIS by Algimantas Bagdonas, Nijolė Meškelienė, Svetlana Ušča, Jana Grava

“…Pedagogo vaidmuo, prisidedant prie ateities kartos ugdymo yra be galo svarbus, todėl ir pats pedagogas turi turėti ne tik plačių profesinių žinių bet ir stiprinti asmeninius ir tarpasmeninius gebėjimus, dar kitaip vadinamus „minkštaisiais“ gebėjimais, kurie padėtų tapti brandžia asmenybe, gebančia spręsti savo problemas, aktyviai veikti darbinėje aplinkoje, gebančia dirbti tiek savarankiškai, tiek grupėje, imtis iniciatyvos ir pan. Straipsnyje keliamas probleminis klausimas – kokios savanoriškos veiklos ir studentų „minkštųjų“ gebėjimų ugdymo(si) sąsajos? …”
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Comparison of the Migration Potential through Microperforated Membranes of CD146+ GMSC Population versus Heterogeneous GMSC Population by Mohamed Al Bahrawy

“…A homogenous CD146 population was compared to heterogeneous GMSCs of origin; the population doubling time and MTT test of the two populations were compared. Migration dynamics were examined in a transwell migration chamber through 8 μm perforated polycarbonic acid membrane, and 0.4 μm and 3 μm perforated collagen-coated polytetrafluoroethylene membrane (PTFE) and 10% fetal bovine serum (FBS) were the chemoattractants used in the lower compartment to induce cell migration, were incubated in a humidified environment for 24 hours, then migrated the cell in the lower compartment examined by a light and electron microscope. …”
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Aqueous Extract of Leaves and Flowers of Acmella caulirhiza Reduces the Proliferation of Cancer Cells by Underexpressing Some Genes and Activating Caspase-3 by Huiny Miriane Tienoue Fotso, Mary-Ann Mbong Angie, Françoise Raïssa Ntentie, Inelle Makamwe, Ferdinand Lanvin Edoun Ebouel, Emmerencia Kenjing Ndansack, Enyong Julius Oben

“…Subsequently, the cytotoxic activity was evaluated on the viability of breast (MCF-7), brain (CT2A, SB-28, and GL-261), colon (MC-38), and skin (YUMM 1.7 and B16-F1) cancer lines using the MTT method. Then, the line where the extract was the most active was selected to evaluate the expression of certain genes involved in cancerogenesis by RT-PCR and the expression of cleaved caspase-3 involved in cell death mechanism by western blot. …”
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IKIMOKYKLINIO UGDYMO SPECIALISTŲ POREIKIS DARBO RINKOJE: PEDAGOGŲ PAIEŠKOS YPATUMAI KAUNO MIESTO UGDYMO ĮSTAIGŲ VADOVŲ POŽIŪRIU by Algimantas Bagdonas, Asta Jakimavičienė, Lina Majauskienė

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Lietuvos valstybės biudžeto skaidrumo ir atskaitingumo principų vertinimas by Giedrė Lapinskienė, Olga Buckiūnienė, Romualda Urniežienė, Rugilė Tamulytė

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Salubrinal Regulates the Apoptosis of Adrenocortical Carcinoma Cells via the PERK/eIF2α/ATF4 Signaling Pathway by Lili Wu, Chunfeng Liang, Xuemei Huang, Xiujun Deng, Jiming Jiang, Zuojie Luo

“…The ACC cell lines, including SW-13 and NCI–H295 R, were used. 3-(4,5)-Dimethylthiazol(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, cell scratch experiments, flow cytometry, and JC-1 staining assays were performed to detect the cell viability, cell migration, and cell apoptosis. …”
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ＤＤＲ１抑制剂与ＩＧＦ－１Ｒ抑制剂联合应用及机制

“…摘 要： 【目的】 研究盘状结构域受体（ＤＤＲ１）的抑制剂３－（２－（吡唑并［１，５－ａ］嘧啶－６－基）乙炔基）苯甲酰胺７ＲＨ对鼻咽癌细胞的体外抗肿瘤作用及相关机制，为该药用于鼻咽癌的临床治疗提供实验依据。【方法】 采用ＭＴＴ法绘制细胞生长曲线，计算７ＲＨ对鼻咽癌细胞的半数抑制浓度（ＩＣ５０值）；Ｗｅｓｔｅｒｎ Ｂｌｏｔ检测７ＲＨ引起鼻咽癌细胞中信号通路的改变，并检测ＳｉＲＮＡ瞬时转染方法干扰ＤＤＲ１后ｐｈｏｓｐｈｏ－ＩＧＦ－１Ｒ的改变。…”
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Formation and stability of green and low-cost magnetoliposomes of the soy lecithin, stigmasterol, and β-sitosterol for hyperthermia treatments by Rosangela Maria Ferreira da Costa e Silva, Ângela Leão Andrade, Erico Tadeu Fraga Freitas, Thalita Marcolan Valverde, Luciano Roni Silva Lara, Darliane Aparecida Martins, Jorge Luis Lopez, Humberto Osório Stumpf, Clésia Cristina Nascentes, Alfredo Miranda de Goes, Rosana Zacarias Domingues

“…Lip-Fe3O4@lecithin with concentrations of 0.58 mg mL−1 of liposome showed high cell viability and are potential candidates for drug delivery and hyperthermia treatments in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.…”
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