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Chemical Modification on Reactive Dye Adsorption Capacity of Castor Seeds
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Use of Thromboelastogram in Venovenous Extracorporeal Membrane Oxygenation for a Patient with Pulmonary Hemorrhage due to Microscopic Polyangiitis
Published 2019-01-01“…Activated clotting time (ACT) or activated partial thromboplastin time (aPTT) has been the mainstay of monitoring of heparin dose. …”
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体外培养的大鼠皮层神经元氧糖剥夺后Sema3A;Nrp-1 mRNA表达上调与轴突网络损伤的关系
Published 2011-01-01“…【方法】 体外培养新生SD大鼠皮层神经元,随机分为正常对照组和OGD处理组:噻唑蓝(MTT)比色法测定神经元活性;βⅢ-tubulin免疫荧光染色观察轴突网络形态学变化,Real-time PCR 检测Sema3A及其受体Nrp-1 mRNA的表达;βⅢ-tubulin免疫荧光染色观察外源性Sema3A蛋白对神经元轴突生长的影响。…”
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Pirfenidone Inhibits Hypoxic Pulmonary Hypertension through the NADPH/ROS/p38 Pathway in Adventitial Fibroblasts in the Pulmonary Artery
Published 2020-01-01“…Proliferation of HPAAFs was quantified by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays to assess cellular metabolic activity, cell counts, and ethynyldeoxyuridine (EdU) assays to detect DNA synthesis. …”
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In Vitro Antibacterial, Antioxidant, Cytotoxicity Activity, and In Silico Molecular Modelling of Compounds Isolated from Roots of Hydnora johannis
Published 2024-01-01“…The cytotoxicity of extracts from roots of H. johannis was conducted against MCF-7 cell lines (human breast cancer) by MTT assay. According to the cytotoxicity study, n-hexane extract exhibited a high level of toxicity with 28.9 ± 5.6% cell viability. …”
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Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism
Published 2025-01-01“…Biofilm biomass and metabolism were assessed using crystal violet and MTT assays and cordycepin cytotoxicity was evaluated in human oral keratinocytes (HOK) using CCK-8 assays. …”
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Biocompatible Heterogeneous Packaging and Laser-Assisted Fluid Interface Control for In Situ Sensor in Organ-on-a-Chip
Published 2024-12-01“…Morphological observations and MTT assays confirmed that the material and the processing methods do not induce cytotoxicity. …”
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Socialinio darbuotojo patiriami sunkumai dirbant su asmenimis, turinčiais priklausomybę
Published 2023-12-01Article -
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Phytochemical Screening and Biological Activity of Mentha × piperita L. and Lavandula angustifolia Mill. Extracts
Published 2018-01-01“…Also, the in vitro antimicrobial effect against Staphylococcus aureus and the antiproliferative activity against two cancerous cell lines (A375 and MDA-MB-231) using the MTT assay were tested. The extracts were prepared using aromatic water which resulted from the extraction of oils from plants as extraction medium, with/without acid. …”
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Nrf2通过上调自噬促进C17.2神经干细胞的增殖
Published 2016-01-01“…我们发现Nrf2能够调控C17.2神经干细胞的增殖,并试图阐明其可能的作用机制?【方法】 采用MTT法检测细胞活力,BrdU掺入法检测细胞增殖,western blot法检测细胞内蛋白水平的变化,流式细胞术检测细胞内ROS水平的变化?…”
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Synthesis of Zinc Oxide (ZnO)-Titanium Dioxide (TiO2)-Chitosan-Farnesol Nanocomposites and Assessment of Their Anticancer Potential in Human Leukemic MOLT-4 Cell Line
Published 2022-01-01“…The cell viability upon treatment was examined by MTT assay. The morphological and nuclear modifications were observed by dual staining. …”
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Cytotoxicity and genotoxicity of orthodontic bands after aging: an in-vitro study
Published 2025-01-01“…Cytotoxicity was assessed using the MTT assay. Comet assay was used to evaluate genotoxicity. …”
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Humanin Rescues Cultured Rat Cortical Neurons from NMDA-Induced Toxicity Not by NMDA Receptor
Published 2014-01-01“…In the present study, excitatory toxicity was induced by N-methyl-D-aspartate (NMDA) in primarily cultured rat cortical neurons. MTT assessment, lactate dehydrogenase (LDH) release, and calcein staining were employed to evaluate the protective activity of humanin on NMDA induced toxicity. …”
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人纤溶酶原K5突变体在大肠杆菌中的优化表达
Published 2008-01-01“…通过电泳分析,获得mK5在原核系统pET22b-BL21的最优表达条件;组氨酸结合柱亲和层析、纯化获得mK5蛋白,SDS-PAGE和Western-blot方法鉴定mK5蛋白;MTT法分析mK5对人视网膜毛细血管内皮细胞(HRCEC)增殖的影响。【结果】 优化后的表达条件为:最适细菌培养温度为37℃,最佳抗生素浓度为50μg/mL羧苄青霉素,最适pH值为7.0,在A600为0.9~1.0时加入终浓度为1.0 mmol/L的IPTG为最佳诱导条件,37℃诱导10 h为最佳诱导时间;优化条件后mK5纯化蛋白得率为21 mg/L,产量较优化前(15 mg/L)提高近30%;mK5特异性地抑制HRCEC的增殖,IC50=40 nmol/L。…”
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Fabrication of Thymoquinone and Ascorbic Acid-Loaded Spanlastics Gel for Hyperpigmentation: In Vitro Release, Cytotoxicity, and Skin Permeation Studies
Published 2025-01-01“…Following that, the optimal spanlastics fomulation loaded with TQ and AA were incorporated into gel and explored for appearance, pH, spreadability, stability, rheology, in vitro release, ex vivo permeation study, and MTT cytotoxicity. The formulated spanlastics gel (R-1) has a pH of 5.53. …”
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