The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity by Saba Dadpour, Amin Mehrabian, Mahdieh Arabsalmani, Elaheh Mirhadi, Anis Askarizadeh, Mohammad Mashreghi, Mahmoud Reza Jaafari

“…Then, in vitro cellular uptake and cytotoxicity were studied by flow cytometry and MTT assay, and compared with commercially available PLD Caelyx®. …”
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Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis by Fatemeh Zare Shahneh, Samira Valiyari, Abbas Azadmehr, Reza Hajiaghaee, Saeid Yaripour, Ali Bandehagh, Behzad Baradaran

“…Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis. …”
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Anticancer Potential of Fruit Extracts from Vatica diospyroides Symington Type SS and Their Effect on Program Cell Death of Cervical Cancer Cell Lines by Atchara Chothiphirat, Kesara Nittayaboon, Kanyanatt Kanokwiroon, Theera Srisawat, Raphatphorn Navakanitworakul

“…The purpose of this study was to investigate the potential cytotoxicity and selectivity of the extracts from V. diospyroides type SS fruit on cervical cancer HeLa and SiHa cell lines and to examine its underlying mechanism of action. MTT assay revealed that the extracts showed inhibition of cell survival in a dose-dependent manner and exhibited highly cytotoxic activity against both HeLa and SiHa cells with IC50 value less than 20 μg/mL along with less toxicity against L929 cells. …”
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慢病毒携带shRNA抑制CR-1基因表达对 CNE-2细胞特性的影响

“…【目的】 应用慢病毒介导的RNA干扰(RNAi)技术,有效沉默鼻咽癌细胞CNE-2的CR-1基因表达,研究CR-1对鼻咽癌细胞生物学行为的影响65377; 【方法】 构建针对CR-1的siRNA慢病毒表达载体[含H1启动子和绿色荧光蛋白(GFP)]65377;利用包装细胞293FT生产慢病毒,感染鼻咽癌细胞CNE-2, 流式筛选GFP阳性细胞65377;应用荧光定量PCR65380;Western blot检测病毒感染后CNE-2细胞中CR-1基因的mRNA和蛋白表达,获得干扰CR-1基因的细胞群65377;并用MTT法65380;平板克隆形成实验及Boyden侵袭小室观察CR-1干扰后对CNE-2细胞生物学行为的影响65377; 【结果】 PCR 和测序证实,成功构建了CR-1 shRNA的慢病毒载体pLVTHM-shCR-165377;荧光定量PCR 和Western blot结果表明,所获得的细胞中CR-1 mRNA及蛋白表达水平较阴性对照组65380;空白对照组均显著降低(P < 0.05)65377;CR-1干扰后的细胞生长明显减慢,克隆形成能力减弱,侵袭运动能力下降65377; 【结论】 通过RNAi技术阻断CR-1的表达,可抑制CNE-2细胞的生长65380;增殖65380;迁徙,提示CR-1在鼻咽癌的发生65380;发展过程中起重要作用65377;…”
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Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258 by Prashantkumar Siddappa Chakra, Aishwarya Banakar, Shriram Narayan Puranik, Vishwas Kaveeshwar, C. R. Ravikumar, Devaraja Gayathri

“…Furthermore, the synthesized nanoparticles displayed significant antibacterial activity against selected human pathogens, with the biggest inhibition zone observed against Staphylococcus aureus (22 ± 0.57 mm) and the smallest inhibition zone observed against Salmonella enterica serovar typhi (3 ± 1 mm). MTT assay revealed the promising antiproliferative potential of ZnO NPs, with an average IC50 value of 98.53 µg/mL. …”
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Slopinimų skaičiavimas optinėje magistralėje su skirstomosiomis atšakomis by Rimantas Plėštys, Jurgita Dabulytė-Bagdonavičienė, Renata Gudaitienė

Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines by Mehmet Ay, Hakan Allı, Alper Önder, Gülce Davutlar, Ferah Cömert Önder

“…In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). …”
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In vitro effects of ascorbic acid on viability and metabolism of patients’ osteosarcoma stem cells by Jovičić Marijana Šimić, Pušić Maja, Antunović Maja, Ledinski Maja, Librenjak Lucija, Kolundžić Robert, Ribičić Tomislav, Trkulja Vladimir, Urlić Inga

“…Cytotoxicity of high-dose AA was confirmed by the MTT test and was proven for all the examined cell types as well as HEK293. …”
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系统性红斑狼疮患者骨髓基质细胞因子表达及对免疫功能的影响 by 邹外一, 童秀珍, 蓝惠霞, 冯炼强, 彭辉, 尹培达, 罗绍凯

“…【方法】采用ELISA方法观察SLE患者骨髓基质细胞培养液IL-6、MIP-1、IFN-γ、TGF-β等细胞因子的表达,采用四甲基偶氮唑盐比色法（MTT）,观察基质细胞培养上清液对外周血淋巴细胞增殖反应的影响。【结果】SLE患者骨髓基质细胞培养液IL-6、MIP-1、IFN-γ浓度明显增高,TGF-β则降低,3种细胞因子与SLEDAI评分相关;正常人及SLE患者骨髓基质细胞上清液均对刀豆蛋白A诱导的外周血淋巴细胞增殖有明显的抑制作用,而SLE患者的上清液抑制作用明显低于正常人;在分别加入抗MIP-1、抗IFN-γ单抗后,骨髓基质细胞上清液对外周血淋巴细胞增殖的抑制作用明显增强,而加入抗TGF-β单抗后,抑制作用明显减弱。…”
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SOCIALINIŲ PASLAUGŲ POREIKIO VERTINIMAS IŠ PASIŪLOS PERSPEKTYVOS: PANEVĖŽIO MIESTO SAVIVALDYBĖS ATVEJIS by Diana Micevičienė

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Exploring Medicinal Plants for Antimicrobial Activity and Synergistic Effects With Doxycycline Against Bacterial Species by Joel Frank Kenmeni, Ibrahim Sifi, Borel Ndezo Bisso, Prudence Ngalula Kayoka-Kabongo, Ulrich Joël Tsopmene, Jean Paul Dzoyem

“…Antibiofilm activity was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. Combination studies were conducted using the checkerboard microdilution method. …”
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Determination of the area index of lettuce leaves with a monocular camera by Laimonas Kairiūkštis, Başak Yalçıner, Emre Özkul

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MicroRNA-1 Inhibits the Growth of Breast Cancer Cells MDA-MB-231 and MCF-7 Treated with Hydatid Cyst Fluid by Hadis Jafari, Mahmoud Mahami-Oskouei, Adel Spotin, Behzad Baradaran, Dariush Shanehbandi, Amir Baghbanzadeh, Zahra Alizadeh

“…The cytotoxicity and cell viability of various HCF concentrations on breast cancer cells were evaluated using the MTT assay. In addition, the expression level of miR-1 in HCF-treated and untreated breast cancer cells was analyzed using qRT-PCR. …”
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鬼臼乙叉苷对人鼻咽癌细胞CNE2的细胞毒作用和对DNA的断裂作用

“…作者用噻唑蓝还原（MTT）法测定了鬼臼乙叉苷（鬼臼乙叉甙,VP-16）对人体鼻咽癌细胞CNE2的毒性作用,在药物浓度为2.5,5.0,10.0,20.0,40.0,80.0和160.0 ug/ml的条件下,连续4次实验,VP-16对CNE2细胞的生长抑制率分别为3.3±3.7％,15.2±3％,36.l±l0％,54±17％,68.4％士13％,76.7士9％和84.2±8％,其半数抑制浓度(IC50)为17.0 ug/ml。…”
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Informacijos sklaida studentams apie psichologinės pagalbos paslaugą Kauno kolegijos atveju by Rasa Gaižiūnaitė, Daiva Stankevičiūtė-Volkauskienė

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Antiproliferative effect of hydroalcoholic brown propolis extract on tumor and non-tumor cells by I. Vendruscolo, G. H. Berton, M. T. Biffi, P. A. Bressiani, A. K. G. Oliveira, A. P. Berti, V. M. Concato-Lopes, W. R. Pavanelli, A. P. Simon, T. L. C. Oldoni, F. A. R. Santos, I. A. L. Dalmolin, M. H. G. Canteri, E. Düsman

“…We aimed to extract compounds of brown propolis with hydroalcoholic solvents and evaluate their cytotoxic activity on tumor and non-tumor cells by MTT test. We tested the solute:solvent ratio (ethanol:water) and extraction time in a Shaker incubator (710 rpm) before conducting a central composite rotational design (CCRD) to optimize time and solvent mixture. …”
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Fragile Histidine Triad (FHIT) Suppresses Proliferation and Promotes Apoptosis in Cholangiocarcinoma Cells by Blocking PI3K-Akt Pathway by Qiang Huang, Zhen Liu, Fang Xie, Chenhai Liu, Feng Shao, Cheng-lin Zhu, Sanyuan Hu

“…The viability and apoptosis of QBC939 cells were then assessed using MTT assays and flow cytometry. Our results revealed that the expression of survivin and Bcl-2 was downregulated, and caspase 3 was upregulated, in cells overexpressing FHIT. …”
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Targeted delivery of curcumin and CM11 peptide against hepatocellular carcinoma cells based on binding affinity of PreS1-coated chitosan nanoparticles to SB3 protein by Danial Rahmani, Ramezan Ali Taheri, Mehrdad Moosazadeh Moghaddam

“…The loading of Cur and CM11 was equal to 87% and 65%, respectively, which had a sustained and better release in the acidic environment than in the physiological environment. The MTT assay showed that PreS1-Cur-CM11-CS NPs act in a targeted and specific manner with the highest toxicity on the HepG2 cells compared to the control by a decrease in viability of about 26% after 48 h based on cell apoptosis. …”
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High Expression of UPK3A Promotes the Progression of Gastric Cancer Cells by Inactivating p53 Pathway by Deliang Xu, Jing Guo, Hongwei Xu

“…In addition, the expression of UPK3A, p53, KLF4, ZMAT3, MDM2, and SP1 was detected by qRT-PCR and Western blot assay. …”
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Design and In Vitro Biological Evaluation of a Novel Organotin(IV) Complex with 1-(4-Carboxyphenyl)-3-ethyl-3-methylpyrrolidine-2,5-dione by Nebojša Đ. Pantelić, Bojana B. Zmejkovski, Željko Žižak, Nebojša R. Banjac, Bojan Đ. Božić, Tatjana P. Stanojković, Goran N. Kaluđerović

“…In vitro anticancer activity of ligand precursor and synthesized organotin(IV) compound was determined against tumor cell lines: human adenocarcinoma (HeLa), human myelogenous leukemia (K562), and human breast cancer (MDA-MB-453), using microculture tetrazolium test (MTT) assay. The results indicate that complex exhibited very high antiproliferative activity against all tested cell lines with IC50 values in the range of 0.22 to 0.53 µM. …”
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