Development of a multiplex loop-mediated isothermal amplification (LAMP) method for differential detection of Mycobacterium bovis and Mycobacterium tuberculosis by dipstick DNA chr... by Mwangala L. Akapelwa, Thoko F. Kapalamula, Lavel C. Moonga, Precious Bwalya, Eddie S. Solo, Joseph Y. Chizimu, Jeewan Thapa, Kyoko Hayashida, Bernard M. Hang'ombe, Musso Munyeme, Aki Tamaru, Takayuki Wada, Shiomi Yoshida, Takuya Kodera, Mistuo Kawase, Stephen V. Gordon, Keiko Yamada, Chie Nakajima, Yasuhiko Suzuki

“…The multiplex LAMP correctly detected MTB and distinguished between M. tuberculosis and M. bovis simultaneously with sensitivities of 500 fg and 1 pg DNA, respectively, within 60 min, and the results were visualized by dipstick chromatography within 10 min. …”
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Meta-analysis of the synergistic effect of traditional Chinese medicine compounds combined with conventional Western medicine in the treatment of osteoporosis by Huan Jin, Huan Jin, Cai Huang, Yan Zhang, Ying Dong, Qi Xiong, Di Wang, Di Wang, Ziyi He, Ziyi He, Lin Shen, Chen Ma, Zixian Wang, Lingfeng Zeng, Bo Shuai

“…This study systematically evaluated the synergistic effect of traditional Chinese medicine (TCM) compounds combined with conventional Western medicine (such as bisphosphonates and calcium) compared to Western medicine alone in the treatment of OP through a meta-analysis.MethodsBased on Preferred Reporting Items for Systematic Reviews and Meta-Analyses and the Measurement Tool for Systematic Review Assessment guidelines, databases such as PubMed and Embase were systematically searched (as of March 2025), and 13 randomized controlled trials (RCTs), involving a total of 2,403 patients, were included.ResultsThe integrated Chinese and Western medicine group showed significantly higher lumbar and femoral neck Bone mineral density (BMD) growth rates than the control group, alongside reduced pain visual analogue scale (VAS) scores. Mechanistically, the combination therapy synergistically modulated bone turnover markers: the bone resorption marker type I collagen C-terminal peptide (CTX-1) decreased (MD = -1.33, P = 0.05), the bone formation marker osteocalcin (OC) increased (MD = 15.56, P < 0.0001), suggesting dual regulation of osteoclast inhibition (e.g., via Receptor Activator of Nuclear Factor-κ B Ligand (RANKL) suppression) and osteoblast activation (e.g., via Wnt/β-catenin signaling). …”
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A Coherent Approach-Based Fine-Tuning of Segment Anything Model Plus Watershed Algorithm for Instance Segmentation of Mitochondria in Electron Microscopy Images by Zahra Faska, Lahbib Khrissi, Imadeddine Mountasser, Khalid Haddouch, Nabil El Akkad, Samah Alshathri, Walid El-Shafai

“…This model would be able to perform precise segmentation for a diverse array of targets across different imaging conditions at the level of performance of specialized U-Net models, or even better. A visual comparison is shown between our fine-tuning SAM model and U-Net, along with an examination of different watershed post-processing strategies to discriminate between adjacent or conjoined instances. …”
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Dietary antioxidants and flavonoids are inversely associated with prostate cancer risk and mortality: evidence from NHANES and machine learning by Yanyang Jin, Dong-shan Lv, Li-po Zhou, Li Xiao, Guang-quan Tong, Abdallah Karim, Shuai Xue, Hongyang Tian, Cheng-cai Wang, Kun Feng, Ding-ming Song, You-liang Guan

“…Shapley Additive Explanations (SHAP) values identified key predictors and visualized their effects.ResultsAmong 2,629 U.S. male participants from NHANES 2007–2010, 144 reported a history of PCa. …”
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Comparación de la riqueza y abundancia de peces arrecifales criptobentónicos en microhábitats coralinos y rocosos en Bahía de los Ángeles, Golfo de California by Luis M Jáquez-Domínguez, Rodrigo D Chiriboga-Ortega, Lydia B Ladah, Luis E Calderón-Aguilera, Omar Valencia-Méndez

“…Este grupo íctico, que mide menos de 5 cm de longitud en su etapa adulta, presenta un camuflaje visual y conductual altamente especializado, lo que a menudo resulta en su subrepresentación en los estudios. …”
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Evaluation of hpv risk groups among women enrolled in the mulher cervical cancer screening study in Mozambique by Cristina Mendes de Oliveira, Ricardina Rangeiro, Nafissa Osman, Ellen Baker, Andrea Neves, Arlete A. N. Mariano, Guilhermina Tivir, Joseph P. Thomas, Jennifer Carns, Viviane Andrade, Carla Carrilho, Eliane C. S. Monteiro, Hannah Hoover, Edson Chivambo, Marcos Chissano, Elizabeth Chiao, Hira Atif, Philip E. Castle, Rebecca Richards-Kortum, Eva Lathrop, Kathleen M. Schmeler, Cesaltina Lorenzoni, Mila P. Salcedo

“…Women with a positive HR-HPV test underwent visual assessment using dilute acetic acid applied to the cervix for treatment decisions. …”
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Eigenvector biomarker for prediction of epileptogenic zones and surgical success from interictal data by Sayantika Roy, Armelle Varillas, Armelle Varillas, Emily A. Pereira, Patrick Myers, Patrick Myers, Golnoosh Kamali, Kristin M. Gunnarsdottir, Kristin M. Gunnarsdottir, Nathan E. Crone, Adam G. Rouse, Jennifer J. Cheng, Michael J. Kinsman, Patrick Landazuri, Patrick Landazuri, Utku Uysal, Carol M. Ulloa, Nathaniel Cameron, Sara Inati, Kareem A. Zaghloul, Varina L. Boerwinkle, Sarah Wyckoff, Niravkumar Barot, Jorge González-Martínez, Joon Y. Kang, Sridevi V. Sarma, Sridevi V. Sarma

“…This process is time-consuming as the clinical team must wait for seizures and visually interpret the iEEG during these events. Hence, an iEEG biomarker that does not rely on seizure observations is desirable to improve EZ localization and surgical success rates. …”
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Identifying key components of a global conceptual framework for adolescent nutrition: Review, nominal group technique and youth co-design by Marion L Roche, Deepika Sharma, Sarah Kehoe, Sara Estecha-Querol, Catharine A K Fleming, Amir A Samnani, Milca J Cameseria, Dipty Chowdhury, Webster Isheanopa Makombe, Amanda Murungi Eunice, Muhammad Sarim Raza, Miriam Shindler, Kesso G van Zutphen-Küffer, Stephanie V Wrottesley

“…It will be designed to be adaptable to different contexts, ensuring its visual design, language and content are accessible and engaging for a broad range of stakeholders.Conclusion Through a novel youth codesign process, we identified key components to inform the development of a new conceptual framework to improve adolescent nutrition. …”
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Early menopause is associated with higher disease activity independent of inflammation in postmenopausal-onset rheumatoid arthritis by Zhi-Ming Ouyang, Yao-Wei Zou, Ye Lu, Jie Pan, Tao Wu, Pei-Wen Jia, Hu-Wei Zheng, Yun Su, Le-Feng Chen, Jian-Zi Lin, Kui-Min Yang, Pei-Yu Lin, Jin-Yuan Han, Jian-Da Ma, Lie Dai

“…Patients-reported outcomes (PROs, included patient global assessment of disease activity [PtGA], pain visual analogue scale [VAS] and Stanford health assessment questionnaire disability index [HAQ-DI]), and PROs-associated indicators (included 28-joint tender joint count [TJC28] and provider global assessment of disease activity [PrGA]) were assessed. …”
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Explainable clinical diagnosis through unexploited yet optimized fine-tuned ConvNeXt Models for accurate monkeypox disease classification by Muhammad Waqar, Zeshan Aslam Khan, Shanzey Tariq Khawaja, Naveed Ishtiaq Chaudhary, Saadia Khan, Khalid Mehmood Cheema, Muhammad Farhan Khan, Syed Sohail Ahmed, Muhammad Asif Zahoor Raja

“…Fortunately, due to the developments in artificial intelligence approaches, it can be implemented to promptly and accurately identify monkeypox disease using visual data information. Many DL driven techniques have already been exploited in the literature for skin related issues, which have provided accurate results to some extent. …”
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Clinical outcomes of limited open reduction and intramedullary nailing with steel cable cerclage in Seinsheimer III femoral subtrochanteric fractures by Hongru Cai, Hongru Cai, Hongru Cai, Hongru Cai, Hongru Cai, XueYi He, XueYi He, XueYi He, XueYi He, XueYi He, Zhengchao Zhang, Zhengchao Zhang, Zhengchao Zhang, Zhengchao Zhang, Zhengchao Zhang, Pinhua Chen, Pinhua Chen, Pinhua Chen, Pinhua Chen, Pinhua Chen, Ruoli Wang, Ruoli Wang, Ruoli Wang, Ruoli Wang, Ruoli Wang, Qi Fang, Qi Fang, Qi Fang, Qi Fang, Qi Fang, Zhixian Xu, Zhixian Xu, Zhixian Xu, Zhixian Xu, Zhixian Xu, Wubing He, Wubing He, Wubing He, Wubing He, Wubing He

“…Surgical parameters (operation time, intraoperative blood loss, and hospital stay), bone healing indicators (callus formation, healing time, and swelling regression), postoperative pain (visual analog scale, VAS), hip function (Harris hip score at 1, 3, and 6 months), and complication rates (coxa vara, implant failure, infection, deep vein thrombosis) were compared. …”
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Critical role of Oas1g and STAT1 pathways in neuroinflammation: insights for Alzheimer’s disease therapeutics by Zhixin Xie, Linxi Li, Weizhong Hou, Zhongxi Fan, Lifan Zeng, Limin He, Yunxiang Ji, Jingbai Zhang, Fangran Wang, Zhou Xing, Yezhong Wang, Yongyi Ye

“…Microglia cells were fixed, permeabilized, blocked, and stained with antibodies for STAT1, then visualized and photographed. Results Bioinformatics and machine learning algorithms revealed that Oas1g was identified as a hub gene, with an AUC of 0.812. …”
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Standardization of Beef, Pork, Chicken, and Soy Protein Extracts for Patch Testing and Their Accuracy in Diagnosing Adverse Food Reactions in Dogs with Chronic Pruritus by Raniere Gaertner, Vanessa Cunningham Gmyterco, Júlia Só Severo, Camilla Alcalá, Maicon Roberto Paulo, Ruan Daros, Marconi Rodrigues de Farias

“…They underwent an elimination diet (ED) and were evaluated using the pruritus visual analog scale (pVAS) and lesion scores (CADESI-4) before and after the ED (days 0 and 45). …”
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Deciphering the anticoccidial mechanism of Qinghao Changshan formula through network pharmacology and molecular docking by Dong Tian, Hui Fu, Hongxia Tao, Miaolan Li, Qinghua Zhang, Weidong Deng

“…Overlapping targets were visualized using Venn diagrams, and protein–protein interaction (PPI) networks were constructed using STRING and Cytoscape. …”
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Clinical effect analysis of unilateral percutaneous vertebral cement distribution in the repair of osteoporotic thoracolumbar vertebral compression fractures by Liu Yuntao, Abuduwupuer Haibier, Aiben Kayierhan, Ma Liang, Yimuran Abudukelimu, Alimujiang Aximu, Tuerhongjiang Abudurexiti, Meng Xiangyu

“…The basic information of patients, surgery-related indicators including operation time, total hospitalization costs, postoperative hospitalization time, cement injection volume, visual analog scale (VAS) for back pain, Oswestry Disability Index (ODI) for back pain, vertebral height restoration rate, local kyphotic angle of the vertebra, and incidence of re-fracture of injured and adjacent vertebrae were compared between the two groups, and the follow-up results of all patients were recorded. …”
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Children’s Headache Through Drawings: A Narrative Review and a Portrait Gallery by Floriana Ferro, Caterina Gaspari, Giulia Manfrè, Federica Cernigliaro, Daniela D’Agnano, Ruben Panzica, Edvige Correnti, Maria Rosita Ruta, Francesca Marchese, Renata Pitino, Mariarita Capizzi, Giuseppe Santangelo, Antonella Versace, Vittorio Sciruicchio, Vincenzo Raieli

“…Our goal is to create a visual reference that can aid clinicians in the accurate interpretation of children’s drawings. …”
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Association between ABCB1 Gene Polymorphisms and Labor Analgesia in Primiparas by Wei Li, Tianke Xiao, Xuehui Wu, Xinyu Wu, Rui Xiang, Haifeng Liu, Jingyi Wang

“…Methods: The cohort comprised 239 primiparas who received epidural analgesia (0.5 μg/L sufentanil + 0.1% ropivacaine). Visual analog scale (VAS) scores were recorded at 0, 1, and 2 h, respectively, after epidural analgesia. …”
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Efecto de un programa de rehabilitación en pacientes con osteoartritis de rodilla by Carlos Eduardo Rangel Galvis, Hilda Marina Wilches, Sandra Zuleta, Paola Díaz, Inés Elvira Gómez Hernández

“…Se realizó una evaluación al inicio del tratamiento y otra a los tres meses para analizar la efectividad del programa, para lo cual se utilizó el índice WOMAC (Western Ontario Mccaster Universities osteoarhritis), el test Timed Up and Go y la escala visual análoga del dolor (EVA). Resultados. La mediana de edad de los participantes fue 69 años y 73,4% eran mujeres. …”
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Associations between serum lipid profiles and the incidence of chronic obstructive pulmonary disease in the UK Biobank by Mengxia Li, Bingqing Xu, Hao Yu, Yanan Wan, Zheng Zhu, Pengfei Luo, Jian Su, Jinyi Zhou, Xikang Fan

“…Restricted cubic spline (RCS) analysis was applied to visualize the dose-response relationship between lipids and COPD risk and Cox proportional hazard models to calculate hazard ratios (HRs) and 95% confidence intervals (CIs). …”
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Ophthalmological manifestations and plasma markers of inflammation in Ebola survivors in post-treatment era by Jean-Claude Mwanza, Alexis K. Kahindo, Justin Mbusa-Kombi, Martial V. Mumbere, Richard O. Kitenge, David R. McIlwain, Jean Christophe S. Mulangu, Placide K. Mbala, Daniel Okitundu, Bruno J. Giordani, Michael J. Boivin, Alla Sikorskii, Nelly N. Kabedi, Jessica G. Shantha, Steven Yeh, Dieudonne N. Mumba, Desire Tshala-Katumbay

“…Survivors with optic neuropathy had significantly worse visual acuity in both eyes than those without optic neuropathy (all p < 0.001). …”
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