Reclassification of Variants Following Renal Genetics Testing: Uncommon Yet Impactful for Diagnosis and Management by Euyn Lim, Chloe Borden, Seysha Mehta, Mary-Beth Roberts, Sarah Mazzola, Fang Zhao, Xiangling Wang

“…Twenty amendments were issued across 19 pedigrees involving 19 variants in 17 genes. …”
Get full text

Multimodal spatial proteomic profiling in acute myeloid leukemia by Christopher P. Ly, Ivo Veletic, Christopher D. Pacheco, Enes Dasdemir, Fatima Z. Jelloul, Sammy Ferri-Borgogno, Akshay V. Basi, Javier A. Gomez, Jessica L. Root, Patrick K. Reville, Sonali Jindal, Sreyashi Basu, Padmanee Sharma, Andres E. Quesada, Carlos Bueso-Ramos, Taghi Manshouri, Branko Cuglievan, Miriam Garcia, Jared K. Burks, Hussein A. Abbas

“…We optimized a high-throughput tissue-microarray-based pipeline for high-plex spatial immunofluorescence and mass cytometry imaging on a single slide, capturing immune, tumor, and structural components. …”
Get full text

Public Database-based Study to Explore the Expression and Role of DDB1 
in Lung Adenocarcinoma by Xinkai ZOU, Ziyi HE, Yanfei ZHANG, Yi HU, Xiaomin WANG, Zhongjie WU

“…Single cell analysis showed that DDB1 was mainly expressed in T cells, alveolar macrophages and dendritic cells. Tissue microarrays confirmed that overall survival was shorter in the DDB1 high expression group (P<0.001), and Cox multifactorial analysis showed that DDB1 was an independent predictor of LUAD prognosis. …”
Get full text

The significance of Trk receptors in pancreatic cancer by Matthew D Johnson, Brandon Stone, Bryan J Thibodeau, Andrew M Baschnagel, Sandra Galoforo, Laura E Fortier, Billie Ketelsen, Samreen Ahmed, Zakiya Kelley, Alaa Hana, Thomas G Wilson, John M Robertson, Robert P Jury, George D Wilson

“…A specific inhibitor of TrkA, B, and C, AstraZeneca 1332, was investigated in vitro and in vivo in combination with radiation. A tissue microarray was constructed from 77 pancreatic ductal adenocarcinoma patients who had undergone neoadjuvant chemoradiation and the Trk receptor, and neurogenic differentiation 1 expression was assessed and correlated with overall survival. …”
Get full text

Overexpression of KMT9α is associated with poor outcome in cholangiocarcinoma patients by Maximilian N. Kinzler, Eric Metzger, Rebecca Schulz, Katrin Bankov, Anna Ramos-Triguero, Falko Schulze, Steffen Gretser, Nada Abedin, Armin Wiegering, Stefan Zeuzem, Dirk Walter, Henning Reis, Roland Schüle, Peter J. Wild

“…For the first time, we investigated the presence of KMT9α in cholangiocarcinoma, the association with histologic subtypes, and its impact on survival. Methods A tissue microarray cohort of all CCA patients who underwent surgical resection with curative intent between 08/2005 and 12/2021 at the University Hospital Frankfurt was immunohistochemically analyzed with the KMT9α antibody. …”
Get full text

Spatial transcriptomics reveal high T cell and monocyte status as predictive and prognostic markers in pancreatic cancer by Yeon Bi Han, Sejoon Lee, Jin-Ok Lee, Se In Jeong, Ki Rim Lee, Soomin Ahn, Kwangrok Jung, Jong-Chan Lee, Yoo-Seok Yoon, Jin-Hyeok Hwang, Ho-Seong Han, Hee Young Na, Jaihwan Kim

“…Methods Forty-eight PDAC cases that were surgically resected with or without NAT were included. A tissue microarray was constructed for DSP and IHC. …”
Get full text

Clinicopathological significance of microRNA‐21 in extracellular vesicles of pleural lavage fluid of lung adenocarcinoma and its functions inducing the mesothelial to mesenchymal t... by Shiori Watabe, Yoshinao Kikuchi, Shigeki Morita, Daisuke Komura, Satoe Numakura, Arisa Kumagai‐Togashi, Masato Watanabe, Noriyuki Matsutani, Masafumi Kawamura, Masanori Yasuda, Hiroshi Uozaki

“…Methods The Cancer Genome Atlas dataset comprising of 448 cases of lung adenocarcinoma, tissue microarray of 144 cases of lung adenocarcinoma, and pleural lavage fluid of 41 cases was used to examine miR‐21 expression levels. …”
Get full text

KRT6A, KRT6B, PKP1, and PKP3 as key hub genes in esophageal cancer: A combined bioinformatics and experimental study by Shayan Marhamati, Morvarid Hamrahjoo, Zeinab Seyedkhan, Mahdi Bahmani, Nasrin Ziamajidi, Iraj Khodadadi, Mohadese Pouryani, Roghayeh Abbasalipourkabir

“…In this study, we downloaded the microarray datasets GSE157808 and GSE92396 from the Gene Expression Omnibus (GEO). …”
Get full text

Therapeutic role of aripiprazole in cartilage defects explored through a drug repurposing approach by Jun-Ku Lee, Hyunjeong Yeo, Sujin Choi, Kyeong Mi Kim, Hannah Kim, Sung-Sahn Lee, Hyun Il Lee, Younghoon Jeong, Hyun-Ju An, Soonchul Lee

“…This study aimed to identify a drug capable of improving cartilage defects by analyzing chondrogenesis-related microarray data from the Gene Expression Omnibus (GEO) public database. …”
Get full text

Diverse regulated cell death patterns and immune traits in kidney allograft with fibrosis: a prediction of renal allograft failure based on machine learning, single-nucleus RNA seq... by Yuqing Li, Jiandong Zhang, Xuemeng Qiu, Yifei Zhang, Jiyue Wu, Qing Bi, Zejia Sun, Wei Wang

“…However, the role of different regulated cell death (RCD) pathways in post-transplant allograft fibrosis remains unclear.Methods and Results: Microarray transcriptome profiling and single-nuclei sequencing data of post-transplant fibrotic and normal grafts were obtained and used to identify RCD-related differentially expressed genes. …”
Get full text

Exogenous TSG-6 treatment alleviates DSS-induced colitis in mice by modulating Pou2f3 and promoting tuft cells differentiation by Shaopeng Yang, Yuqi Li, Rongwei Ruan, Jiangping Yu, Bo Zhu, Haibin Lou, Xiaolan Zhang, Shi Wang

“…Results TSG-6 treatment significantly attenuated DSS-induced colitis symptoms and inflammatory cell infiltration. Microarray analysis revealed that TSG-6 decreased pro-inflammatory cytokine levels in colon tissue. …”
Get full text

Identification and characterization of the anti-viral interferon lambda 3 as direct target of the Epstein-Barr virus microRNA-BART7-3p by Juliane Blümke, Marcus Bauer, Christoforos Vaxevanis, Andreas Wilfer, Ofer Mandelboim, Claudia Wickenhauser, Barbara Seliger, Simon Jasinski-Bergner

“…The effect of ebv-miR-BART7-3p on the IFNL3 expression was validated both in human cell lines in vitro and in human tissue specimen with known EBV status. These results expand the current knowledge of EBV-encoded miRs and their role in immune evasion, pathogenesis and malignant transformation.…”
Get full text

Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma by Yan Lu, Shuang Zhou, Han Wang, Fangming Liu, Weiren Liu, Chunhui Yang, Mengxin Tian, Guangshuai Jia, Bijun Zhu, Mingxiang Feng, Tiankui Qiao, Xinxin Wang, Wei Cao, Xiangdong Wang, Yinghong Shi, Duojiao Wu

“…Immunohistochemistry staining on the tissue microarray of 118 patients with HCC showed intra-tumoral FABP5 high CD8+ T-cell infiltration was linked to overall and recurrence-free survival.Conclusions The tumor microenvironment can impose metabolic restrictions on T-cell function. …”
Get full text

E3 ligase HERC5-catalyzed UGDH isgylation promotes SNAI1-mediated tumor metastasis and cisplatin resistance in oral squamous cell carcinoma by Xu Zhang, Fayu Liu, Qigen Fang, Changfu Sun, Jie Fan

“…Previous studies have revealed an important function of HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5 (HERC5) in cancer. Six GEO gene microarrays identified HERC5 as a significant upregulated gene in OSCC tissues or cells (log2 Fold change > 1 and adj.p < 0.05). …”
Get full text

Unveiling the impact of C15orf48 on non-small cell lung cancer through NF-kappa B signaling by Wei Wang, Lei Zhang, Ansheng Wang, Xiaohua Wang, Weidong Wu

“…Immunohistochemical staining was used to detect C15orf48 expression in tissue microarrays. Cellular assays, including CCK8, colony formation, wound healing, transwell migration, flow cytometry, and cell adhesion, were conducted to assess cell viability, proliferation, invasion, and apoptosis. …”
Get full text

Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G. by Robert A Eagle, Gillian Flack, Anthony Warford, Jesús Martínez-Borra, Insiya Jafferji, James A Traherne, Maki Ohashi, Louise H Boyle, Alexander D Barrow, Sophie Caillat-Zucman, Neil T Young, John Trowsdale

“…Using specific anti-RAET1G1 antiserum to stain tissue microarrays we show that RAET1G1 expression is highly restricted in normal tissues. …”
Get full text

TRIM21 functions as an oncogene in glioblastoma by transactivating FOSL1 and promoting the ubiquitination of p27 by Vanajothi Ramar, Rajveer Singh Sidhu, Osei Pako, Cheyenne R. Cisse, Alyssa A. Guo, Jason Li, Kelcey Stapleton, Shanchun Guo, Guangdi Wang, Mingli Liu

“…Immunohistochemistry on glioma patient tissue microarray (TMA) assessed TRIM21 expression and its association with FOSL1, IDH status, and glioma grade. …”
Get full text

Nanopore-based random genomic sampling for intraoperative molecular diagnosis by Francesco E. Emiliani, Abdol Aziz Ould Ismail, Edward G. Hughes, Gregory J. Tsongalis, George J. Zanazzi, Chun-Chieh Lin

“…This streamlined workflow—from tissue arrival to automatic generation of CNV and methylation reports—can be completed within 105 min. …”
Get full text

Genome-wide analysis in the search for candidate genes associated with meat productivity traits in meat-and-dairy goats by M. I. Selionova, A.-M. M. Aibazov, A. A. Sermyagin, A. A. Belous, N. A. Zinovieva

“…GWAS analysis using Goat 50K BeadChip high-density DNA microarray determined a genome-wide level of significance for six SNPs located on chromosomes 5, 6, 10 and 16 associated with the live weight of young animals (4 and 8 months old). …”
Get full text

Deciphering the methylation landscape of long noncoding RNAs in hepatocellular carcinoma: a focus on LINC00942 by Zhaoqi Shi, Xiaolong Liu, Duguang Li, Jing Yang, Haonan Chen, Hui Lin, Xiaoxiao Fan

“…Methods We conducted a methylation microarray analysis on 11 HCC samples alongside adjacent non-tumor tissue specimens. …”
Get full text