Pattern specification and immune response transcriptional signatures of pericardial and subcutaneous adipose tissue. by Frank H Lau, Rahul C Deo, Gregory Mowrer, Joshua Caplin, Tim Ahfeldt, Adam Kaplan, Leon Ptaszek, Jennifer D Walker, Bruce R Rosengard, Chad A Cowan

“…To better characterize the role of PCAT in the pathogenesis of disease, we performed a large-scale unbiased analysis of the transcriptional differences between PCAT and subcutaneous adipose tissue, analysing 53 microarrays across 19 individuals. …”
Get full text

Rapid Whole-Genome Sequencing in Critically Ill Infants and Children with Suspected, Undiagnosed Genetic Diseases: Evolution to a First-Tier Clinical Laboratory Test in the Era of... by Rina Kansal

Get full text

The role of sialyl glycan recognition in host tissue tropism of the avian parasite Eimeria tenella. by Livia Lai, Janene Bumstead, Yan Liu, James Garnett, Maria A Campanero-Rhodes, Damer P Blake, Angelina S Palma, Wengang Chai, David J P Ferguson, Peter Simpson, Ten Feizi, Fiona M Tomley, Stephen Matthews

“…EtMIC3 consists of seven tandem MAR1-type domains, which possess a high specificity for sialylated glycans as shown by cell-based assays and carbohydrate microarray analyses. The restricted tissue staining pattern observed for EtMIC3 in the chicken caecal epithelium indicates that EtMIC3 contributes to guiding the parasite to the site of invasion in the chicken gut. …”
Get full text

Acute Changes in Rat Tissue Gene Expression Following Exposure to Flight Relevant Hypobaria by Apurva Borcar, Flaubert Tchantchou, Amol C. Shetty, Anup Mahurkar, Gary Fiskum

Get full text

Brain-specific rescue of Clock reveals system-driven transcriptional rhythms in peripheral tissue. by Michael E Hughes, Hee-Kyung Hong, Jason L Chong, Alejandra A Indacochea, Samuel S Lee, Michael Han, Joseph S Takahashi, John B Hogenesch

“…The circadian regulatory network is organized in a hierarchical fashion, with a central oscillator in the suprachiasmatic nuclei (SCN) orchestrating circadian oscillations in peripheral tissues. The nature of the relationship between central and peripheral oscillators, however, is poorly understood. …”
Get full text

Identification of potential biomarkers and pathways related to major depressive disorder by integrated bioinformatic analysis and experimental validation by Ying Zeng, Lu-Qi Peng, Mei Zhang, Rong Zhong, Ke-Chao Nie, Wei Huang

“…Objective: To identify promising biomarkers for the pathogenesis of major depressive disorder (MDD). Methods: Microarray chips of MDD patients, including the GSE98793, GSE52790, and GSE39653 datasets, were obtained from the Gene Expression Omnibus database. …”
Get full text

Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis by CONG Shan, SUI Wei-guo, ZOU Gui-mian, XUE Wen, LI Huan, YAN Qiang, CHEN Jie-jing, LUO Ya-dan, CHEN Huai-zhou

“…Objective To study differential expression profile of mRNA and long non-coding RNA(IncRNA) through microarray analysis between non-IgA mesangial proliferative glomerulonephritis(MsPGN) patients and the controls,and then explore the potential role of IncRNA in the pathogenesis of non-IgA MsPGN.Methods Through simple random sampling,4 patients with non-IgA MsPGN and 2 controls were selected as disease group and control group,respectively.Renal cortical tissues from two groups were collected.Total RNA was extracted,quantified and prepared to ds-cDNA through reverse transcription ds-cDNA was labeled with NimbleGen one-color DNA labeling kit and used for array hybridization.All experimental data were processed through GO analysis,Pathway analysis and the gene loci correlation analysis of mRNA and IncRNA.Some IncRNAs that were closely related to non-IgA MsPGN were screened out.Finally,part of the array results was detected by PCR to verify the reliability of array test Results By fold change filtering,4317 differentially expressed mRNAs and 3502 differentially expressed IncRNAs were screened out.Five IncRNAs were found to play potential roles in the pathogenesis of non-IgA MsPGN:AF1180924(close to coding gene FGG),AK092233(close to coding gene COL18A1),AK130579(close to coding gene CREBBP),AK023598(close to coding gene LEPR),and AK055915(close to coding gene CDC42EP3).These results provided an important basis for revealing the pathogenesis of non-IgA MsPGN.Conclusions Some IncRNAs can potentially regulate related genes and plays an important role in the pathogenesis and development of non-IgA MsPGN.…”
Get full text

Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediate... by Muhammad Anas Ramzan, Zhedong Yang, Li Cao, Jinyuan Zhang, Fei Wu, Miaomiao Xin, Bo Guo, Lumin Wang, Lingyu Zhao, Yannan Qin, Chengcheng Liu

“…Abstract Infertility is a global issue, with male factors contributing to approximately 50% of cases. …”
Get full text

A Sister Mary Joseph’s nodule in fallopian tube cancer: exploring the metastatic pathway through gene expression profiling—a case report by Takuto Uyama, Yusuke Matoba, Hikaru Nakahara, Akira Ishikawa, Hiroaki Niitsu, Kosuke Nakamoto, Iemasa Koh, Shinji Mii, Takao Hinoi, Kouji Banno

“…Metastatic high-grade serous carcinoma was identified in the lymph nodes and umbilical tissue. Tumor tissue samples were collected from the primary lesion, umbilical metastasis, and inguinal lymph node metastasis for RNA microarray analysis. …”
Get full text

Metabolomic and flux‐balance analysis of age‐related decline of hypoxia tolerance in Drosophila muscle tissue by Laurence Coquin, Jacob D Feala, Andrew D McCulloch, Giovanni Paternostro

“…We expand the reactions in the model using tissue‐ and age‐specific microarray data from the literature, and then examine metabolomic profiles of thoraxes after 4 h at 0.5% O2 and after 5 min of recovery in 40‐ versus 3‐day‐old flies. …”
Get full text

The prognostic significance of MMP-8 tissue Immunoexpression in pancreatic ductal adenocarcinoma after neoadjuvant therapy by Ella Kesti, Annika Eurola, Mirjami Kaasinen, Harri Mustonen, Jaana Hagström, Timo Sorsa, Ari Ristimäki, Hanna Seppänen, Caj Haglund, Malin Sund

“…We studied MMP-8 expression using immunohistochemistry in a tissue microarray with samples from 115 NAT and 144 up-front surgery patients. …”
Get full text

Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation by Jonathan Tang, Yurong Huang, David H. Nguyen, Sylvain V. Costes, Antoine M. Snijders, Jian-Hua Mao

“…However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. …”
Get full text

Phosphoribosyl transferase domain containing 1: A prognostic biomarker in testicular germ cell tumors by Peisheng Huang, Yihao Chen, Yongcheng Shi, Chuanfan Zhong, Huawei Lin, Xiaoxue Yu, Kai Chen, Zhuoya Huang, Le Zhang, Shumin Fang, Jianming Lu, Jiahong Chen

“…Immunohistochemistry on tissue microarrays validated PRTFDC1’s predictive value in clinical samples. …”
Get full text

The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells by Haruna Kashio, Rie Takai, Ayuko Takada, Yuya Nakao, Nattakarn Hosiriluck, Masahiro Iijima, Yoshihiro Abiko, Itaru Mizoguchi, Toshiya Arakawa

“…Cells derived from the TMJ discs were cultured, and mRNA extracted from the disc tissues and derived cells was analyzed for gene profiling via microarray hybridization. …”
Get full text

Genomic and functional studies of Drosophila sex hierarchy regulated gene expression in adult head and nervous system tissues. by Thomas D Goldman, Michelle N Arbeitman

“…We also identified 90 and 26 genes regulated in the adult head and central nervous system tissues, respectively, downstream of the sex-specific transcription factors encoded by fru. …”
Get full text

Molecular profiles of HCV cirrhotic tissues derived in a panel of markers with clinical utility for hepatocellular carcinoma surveillance. by Ricardo C Gehrau, Kellie J Archer, Valeria R Mas, Daniel G Maluf

“…L(1)-penalized logistic regression model was fit for woHCC and wHCC microarrays, and tested against iHCC. Prediction model genes were validated in independent set by qPCR. …”
Get full text

Assessing the suitability of formalin-fixed paraffin-embedded (FFPE) tissue for genome-wide association studies (GWAS) by Julian Reingruber, Maximilian J. Krämer, Jessica Bigge, Pouria Dasmeh, Sandeep Grover, Stefanie Heilmann-Heimbach, Markus M. Nöthen, Nicole Kreuser, Ines Gockel, Lothar Veits, Michael Vieth, Paul Jank, Carsten Denkert, Carlo Maj, Timo Hess, Johannes Schumacher

“…This, together with the lower cost of genotyping and computational efficiency, makes microarray technology currently the superior method for GWAS using FFPE tissue. …”
Get full text

LOSS OF HETEROZYGOSITY IN THE BRCA1 AND BRCA2 LOCUS IN BREAST CANCER by M. M. Tsyganov, M. K. Ibragimova, A. M. Pevzner, N. V. Litviakov

“…To assess the status of LOH, microarray analysis was performed on high-density DNA chips from Affymetrix CytoScanTM HD Array. …”
Get full text

In Vitro Investigation of Statin Effects on Genes Associated with Severe COVID-19 in Cancerous and Non-Cancerous Cells by Adriana Kapustová, Patrik Macášek, Bibiána Baďurová, Jana Melegová, Silvie Rimpelová, Jan Kubovčiak, Jana Šáchová, Miluše Hradilová, Michal Kolář, Libor Vítek, Tomáš Ruml, Helena Gbelcová

“…<b>Methods:</b> We extended the interpretation of the whole-genome DNA microarray analyses of pancreatic cancer cells MiaPaCa-2 and whole-transcriptome analyses of adipose tissue-derived mesenchymal stem cells AD-MSC that we had performed in the past. …”
Get full text

A stratified transcriptomics analysis of polygenic fat and lean mouse adipose tissues identifies novel candidate obesity genes. by Nicholas M Morton, Yvonne B Nelson, Zoi Michailidou, Emma M Di Rollo, Lynne Ramage, Patrick W F Hadoke, Jonathan R Seckl, Lutz Bunger, Simon Horvat, Christopher J Kenyon, Donald R Dunbar

“…<h4>Results</h4>To enrich for adipose tissue obesity genes a 'snap-shot' pooled-sample transcriptome comparison of key fat depots and non adipose tissues (muscle, liver, kidney) was performed. …”
Get full text