Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data by Ting-qing Gan, Zu-cheng Xie, Rui-xue Tang, Tong-tong Zhang, Dong-yao Li, Zu-yun Li, Gang Chen

“…First, a meta-analysis was performed with eligible literature, followed by microRNA microarrays in the Gene Expression Omnibus database, to verify the diagnostic and prognostic values of miR-145-5p. …”
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Survivin Expression in Placentas with Intrauterine Growth Restriction by Pavo Perković, Sanja Štifter-Vretenar, Marina Perković, Marko Štefančić, Ena Holjević, Andrea Dekanić, Tea Štimac

“…Tissue microarrays (TMAs) were constructed, and survivin expression was assessed using immunohistochemistry (IHC). …”
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Identification and Cross-Platform Validation of Sparse Molecular Classifiers for Antibody-Mediated and T-Cell–Mediated Rejection After Kidney Transplantation by Jasper Callemeyn, Josué Manik Nava-Sedeño, Dany Anglicheau, Jack Beadle, Jan Hinrich Bräsen, Marian C. Clahsen-van Groningen, Iacopo Cristoferi, Henriette de Loor, Andreas Deutsch, Marie Essig, Wilfried Gwinner, Philip F. Halloran, Dennis A. Hesselink, Priyanka Koshy, Dirk Kuypers, Evelyne Lerut, Pierre Marquet, Robert C. Minnee, Candice Roufosse, Ben Sprangers, Amaryllis H. Van Craenenbroeck, Haralampos Hatzikirou, Maarten Naesens

“…Herein, we aimed to develop and validate sparse molecular classifiers for ABMR and TCMR using allograft tissue. Methods: In a discovery cohort of 224 kidney transplant biopsies, lasso regression was applied on microarray gene expression data to derive a molecular classifier for ABMR and TCMR, respectively. …”
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Gene Expression Profiling in Wild-Type and PPAR𝛼-Null Mice Exposed to Perfluorooctane Sulfonate Reveals PPAR𝛼-Independent Effects by Mitchell B. Rosen, Judith R. Schmid, J. Christopher Corton, Robert D. Zehr, Kaberi P. Das, Barbara D. Abbott, Christopher Lau

“…Perfluorooctane sulfonate (PFOS) is a perfluoroalkyl acid (PFAA) and a persistent environmental contaminant found in the tissues of humans and wildlife. Although blood levels of PFOS have begun to decline, health concerns remain because of the long half-life of PFOS in humans. …”
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CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer by Xiao Ma, Baojian Zhang, Xuezhe Yin, ShiPeng Yang, Zhenhua Lin, Yang Yang, Xianchun Zhou

“…Finally, the prognostic significance of CPT1A and the vascular marker VEGF in BC tissues was evaluated using tissue microarrays and public databases. …”
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Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma by Qing Yuan, Song‐Quan Wang, Guang‐Tao Zhang, Jie He, Zhi‐Dan Liu, Ming‐Rong Wang, Hong‐Qing Cai, Jing‐Hai Wan

“…Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. …”
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Targeting Mesothelin Enhances Personalized Neoantigen Vaccine Induced Antitumor Immune Response in Orthotopic Pancreatic Cancer Mouse Models by Zhixiong Cai, Zhenli Li, Wenting Zhong, Fangzhou Lin, Xiuqing Dong, Honghao Ye, Yutong Guo, Geng Chen, Xiaoling Yu, Haijun Yu, Ruijing Tang, Xiaolong Liu

“…Here, three datasets and multi‐immunofluorescence staining of tissue microarrays in pancreatic cancer indicate that mesothelin (MSLN) expression negatively correlates with cytotoxic T cells in tumor. …”
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Comprehensive analysis of single-cell and bulk RNA sequencing data unveils antigen-presenting and processing fibroblasts and establishes a predictive model in gastric cancer by Chenggang Zhang, Fangqi Chen, Jie Li, Yixuan He, Juan Sun, Zicheng Zheng, Guanmo Liu, Yihua Wang, Weiming Kang, Xin Ye

“…Immunohistochemistry of GC tissue microarrays was performed to validate the model. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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Tumor-infiltrating B cells produce tumor-specific antibodies and may contribute to suppressing tumor in head and neck squamous cell carcinoma by Junsei Sameshima, Hu Chen, Naoki Kaneko, Lijing Yan, Shiho Yokomizo, Tomoki Sueyoshi, Haruki Nagano, Taiki Sakamoto, Shoichi Tanaka, Yasuyuki Maruse, Taichi Hattori, Ryoji Kitamura, Yoshikazu Hayashi, Takashi Maehara, Shinsuke Fujii, Tamotsu Kiyoshima, Thomas Guy, Zivile Giedraityte, Wataru Kumamaru, Masafumi Moriyama, Shintaro Kawano

“…These ASCs exhibited a low somatic hypermutation frequency, indicating that they are likely activated through extrafollicular response rather than germinal center response. Protein microarrays with recombinant antibodies mimicking TIL-Bs secretory antibodies and serum antibodies revealed several antigens highly expressed in tumor cells. …”
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Co-regulation of the DAF-16 target gene, cyp-35B1/dod-13, by HSF-1 in C. elegans dauer larvae and daf-2 insulin pathway mutants. by Wendy B Iser, Mark A Wilson, William H Wood, Kevin Becker, Catherine A Wolkow

“…C. elegans IIS controls expression of a number of stress response genes, which were differentially regulated by tissue-restricted IIS. In particular, expression of sod-3, a MnSOD enzyme, was not regulated by tissue-restricted IIS on the microarrays, while expression of hsp-16 genes was rescued back to wildtype by tissue restricted IIS. …”
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Comprehensive Expression Profiling and Molecular Basis of CDC28 Protein Kinase Regulatory Subunit 2 in Cervical Cancer by Li Qin, Xiaoqiong Luo, Xiao Qin, Hongbao Huang, Lianling Zhang, Shengcai Chen, Xiaoqin Wu, Bingsheng Huang, Jian Pan, Jingxi Wei

“…Herein, taking advantage of massive genetics data from multicenter RNA-seq and microarrays, we were the first group to perform tissue microarrays for CKS2 in cervical cancer. …”
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RNA profiling and immunohistochemistry analyses of circRNAs in imatinib-resistant gastrointestinal stromal tumors by Hanxing Tong, Ning Jia, Wenyang Li, Jingjing Xu, Qiuyue Li, Xiaomeng He, Huaqin Sun, Christopher Corpe, Jin Wang

“…Methods To identify the factors that are responsible for imatinib resistance, we investigated the differentially expressed mRNAs and circRNAs in imatinib-naïve and imatinib-resistant GISTs via ceRNA microarrays. The expression levels of circ-BRIP1, circ-EPHB4 and their host genes were validated via quantitative real-time PCR analyses and formalin-fixed and paraffin-embedded (FFPE) tissue microarrays (TMAs). …”
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Impact of MiRNAs on Wnt-related gene activity in breast cancer by Tomasz Sirek, Agata Sirek, Nikola Zmarzły, Marcin Opławski, Katarzyna Król-Jatręga, Dariusz Boroń, Michał Chalcarz, Piotr Ossowski, Konrad Dziobek, Damian Strojny, Joanna Szymańska, Julia Gajdeczka, Przemysław Borawski, Kacper Boroń, Beniamin Oskar Grabarek

“…Expression profile of Wnt-related genes was assessed with mRNA microarrays and reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
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Decoding the prognostic landscape of LUAD: the interplay between N6-methyladenosine modification and immune microenvironment by Quan Chen, Quan Chen, Weijun Wan, Qing Zhao, Juan Li, Juan Li, Yanli Xiong, Yanli Xiong, Yuchuan Yuan, Lu Tang, Xiaofeng Wu, Wei Xing, Wei Guo, Di Lu, Luoquan Ao, Xiang Xu, Xiang Xu, Xiang Xu, Xiang Ao, Xiang Ao, Xiang Ao

“…These findings were validated by two independent databases and LUAD tissue microarrays. METTL3 was found to impact the mRNA expression of these genes, with METTL3 deficiency abolishing these interactions. …”
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Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model by Min Li, Ying Yuan, Qingzhong Chen, Rao Me, Qing Gu, Yunjie Yu, Minjie Sheng, Bilian Ke

“…Results. For genome microarrays, the expression of Wnt3 in FDM group was significantly greater as confirmed in retinal and scleral tissue. …”
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Laminin N-terminus α31 is upregulated in invasive ductal breast cancer and changes the mode of tumour invasion. by Lee D Troughton, Danielle A O'Loughlin, Tobias Zech, Kevin J Hamill

“…LaNt α31 expression and distribution were analysed by immunohistochemistry in human breast tissue biopsy sections and tissue microarrays covering 232 breast cancer samples. …”
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Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction by Van Luu-The, Nathalie Paquet, Ezequiel Calvo, Jean Cumps

“…Quantification of mRNA expression levels using real-time reverse transcription PCR (RT-PCR) is increasingly used to validate results of DNA microarrays or GeneChips®. It requires an improved method that is more robust and more suitable for high-throughput measurements. …”
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Non-viral HCC miRNA profiling reveals miR-1972 as a potential positive prognostic marker by Venkata Ramana Mallela, Marie Rajtmajerová, Esraa Ali, Lenka Červenková, Andriy Trailin, Petr Hošek, Richard Pálek, Ondřej Daum, Václav Liška, Kari Hemminki, Filip Ambrozkiewicz

“…Methods: We conducted microRNA profiling by microarrays in 45 paired (tumor and non-tumor adjacent tissue) samples from non-viral HCC patients. …”
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ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma by Haoyuan Xu, Wanpeng Li, Kai Xue, Huankang Zhang, Han Li, Haoran Yu, Li Hu, Yurong Gu, Houyong Li, Xicai Sun, Quan Liu, Dehui Wang

“…Through comprehensive miRNA sequencing, tumor tissue microarrays and tissue samples analysis, we identified miR-142-3p as a significantly upregulated miRNA that is strongly associated with poor prognosis in recurrent NPC patients. …”
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