P67 | EMERGING ROLE OF NUCLEAR PHOSPHOLIPASE C BETA 1 AS POTENTIAL BIOMARKER IN LUNG CANCER

“…PLCβ1 displayed favorable results, therefore its expression was evaluated through immunohistochemistry on a commercial tissue microarray. Notably, the array revealed a consistent nuclear overexpression of the enzyme in tumoral tissue of patients. …”
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Xirp proteins mark injured skeletal muscle in zebrafish. by Cécile Otten, Peter F van der Ven, Ilka Lewrenz, Sandeep Paul, Almut Steinhagen, Elisabeth Busch-Nentwich, Jenny Eichhorst, Burkhard Wiesner, Derek Stemple, Uwe Strähle, Dieter O Fürst, Salim Abdelilah-Seyfried

“…Myocellular regeneration in vertebrates involves the proliferation of activated progenitor or dedifferentiated myogenic cells that have the potential to replenish lost tissue. In comparison little is known about cellular repair mechanisms within myocellular tissue in response to small injuries caused by biomechanical or cellular stress. …”
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Inhibition of dipeptidyl peptidase-4 ameliorates cardiac ischemia and systolic dysfunction by up-regulating the FGF-2/EGR-1 pathway. by Masayoshi Suda, Ippei Shimizu, Yohko Yoshida, Yuka Hayashi, Ryutaro Ikegami, Goro Katsuumi, Takayuki Wakasugi, Yutaka Yoshida, Shujiro Okuda, Tomoyoshi Soga, Tohru Minamino

“…DNA microarray analysis revealed that the cardiac tissue level of early growth response protein 1 (EGR-1), which activates angiogenesis, was significantly reduced in untreated mice with dietary obesity, while this decrease was inhibited by administration of linagliptin. …”
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In vivo RNAi screen and validation reveals Ngp, Hba-a1, and S100a8 as novel inhibitory targets on T lymphocytes in liver cancer by Inga Hochnadel, Lisa Hoenicke, Nataliia Petriv, Huizhen Suo, Lothar Groebe, Chantal Olijnik, Nina Bondarenko, Nina Bondarenko, Juan C. Alfonso, Michael Jarek, Ruibing Shi, Andreas Jeron, Andreas Jeron, Kai Timrott, Tatjana Hirsch, Nils Jedicke, Dunja Bruder, Dunja Bruder, Frank Klawonn, Frank Klawonn, Frank Klawonn, Ralf Lichtinghagen, Robert Geffers, Henrike Lenzen, Henrike Lenzen, Michael P. Manns, Tetyana Yevsa

“…Therefore, in this study, we aimed to define novel ICI molecules arising on T cells during aggressive HCC development.MethodsUsing autochthonous HCC models, we performed microarray analyses followed by in vivo RNA interference screen and identified several new ICI molecules on CD4 and CD8 T lymphocytes in HCC-bearing mice. …”
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CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling by Zihao Wei, Ying Wang, Jiakuan Peng, Honglin Li, Junjie Gu, Ning Ji, Taiwei Li, Xikun Zhou, Xin Zeng, Jing Li, Qianming Chen

“…Here, we demonstrated that the circRFWD3 was significantly upregulated in HNSCC tissues and cell lines by circRNA microarray analysis and qPCR. …”
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Evaluation of Organoid‐Derived Exosomal microRNA as Liquid Biopsy for Colorectal Cancer: A Multicenter Cross‐Sectional Study by Atsuhiro Sasaki, Masatake Kuroha, Masaki Tosa, Seiichi Takahashi, Shinya Oomori, Eiki Nomura, Tatsuya Kikuchi, Motoyuki Onodera, Yuichiro Sato, Teruko Miyazawa, Hirofumi Chiba, Naonobu Yokoyama, Jun Kusaka, Daisuke Okamoto, Tomoyuki Handa, Mikako Sugimura, Masahiro Iwabuchi, Keiichiro Hiramoto, Hidekazu Shirota, Hideya Iwaki, Hiroshi Nagai, Yusuke Shimoyama, Takeo Naito, Rintaro Moroi, Hisashi Shiga, Yoichi Kakuta, Yoshitaka Kinouchi, Atsushi Masamune

“…Organoids were established from normal colon and colorectal cancer tissues. Exosomes were isolated from conditioned media. miRNAs were extracted from exosomes and compared using microarray analysis. …”
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E2F2(E2F transcription factor 2) as a potential therapeutic target in meibomian gland carcinoma: evidence from functional and epigenetic studies by Wei Wang, Hetong Wang, Xun Liu, Fei Xu, Qin Tang, Chuanli Zhang, Jiaqi Lin, Limin Zhu, Tingting Lin

“…Methods E2F2 expression was measured in MGC cells and tissues. Tissue samples from 3 normal meibomian glands (MG) and 36 MGC patients were used to construct a tissue microarray. …”
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Inhibitory Effect of PRMT5/MTA Inhibitor on MTAP‐Deficient Glioma May Be Influenced by Surrounding Normal Cells by Yunjie Wang, Xiaohui Sun, Runchen Ma, Xiaofan Zhang, Shengmin Ji, Zhaofeng Liu, Gangqiang Yang, Hongbo Wang, Peng Zhang, Jianzhao Zhang, Jingwei Tian

“…Methods We first analyzed whether there were MTAP‐expressing normal intracerebral cells around MTAP‐deficient glioma tissues by paraffin‐embedded tissue microarray of human glioma specimens. …”
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miR-361-5p contributes to the pathogenesis of Alzheimer’s disease by Abbas Jalaiei, Jalal Gharesouran, Shahram Arsang-Jang, Mahnaz Talebi, Maryam Rezazadeh, Soudeh Ghafouri-Fard

“…This study investigated the roles of HOMER1, ATAD1, and miR-361 in AD pathogenesis using microarray (GSE106241, GSE157239; n = 60) and RT-PCR (n = 100; 50 AD patients, 50 controls from Northwest Iran) analyses. …”
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Prognostic value of immune biomarkers in melanoma loco-regional metastases. by Emilia Hugdahl, Sura Aziz, Tor A Klingen, Lars A Akslen

“…In a patient series of loco-regional metastases from nodular melanomas (n = 78; n = 26 skin metastases, n = 52 lymph node metastases), expression of PD-L1 in tumour cells and the number of CD3, CD8, FOXP3 and PD-L1 positive TILs were determined by immunohistochemistry on tissue microarray (TMA) slides. Due to limited tumour tissue in the paraffin blocks, 67 of the 78 cases were included for tissue microarrays. …”
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Gene Expression Changes Induced by PPAR Gamma Agonists in Animal and Human Liver by Alexandra Rogue, Catherine Spire, Manuel Brun, Nancy Claude, André Guillouzo

“…Several studies have been conducted using PCR analysis and microarray technology to identify possible target genes and here we review the data obtained from various in vivo and in vitro experimental models. …”
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Endothelial angiogenesis is directed by RUNX1T1-regulated VEGFA, BMP4 and TGF-β2 expression. by Ko-Hsun Liao, Shing-Jyh Chang, Hsin-Chuan Chang, Chen-Li Chien, Tse-Shun Huang, Te-Chia Feng, Wen-Wei Lin, Chuan-Chi Shih, Muh-Hwa Yang, Shung-Haur Yang, Chi-Hung Lin, Wei-Lun Hwang, Oscar K Lee

“…Tissue angiogenesis is intimately regulated during embryogenesis and postnatal development. …”
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Brain gliomas new transcriptomic discoveries from differentially expressed genes to therapeutic targets by Shiwen Pei, Zhiquan Jiang, Hongwei Cheng

“…This study aimed to identify new genetic targets linked to glioma by analyzing microarray datasets to uncover genetic factors involved in its onset and progression. …”
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Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients. by Meredith G Banigan, Patricia F Kao, James A Kozubek, Ashley R Winslow, Juan Medina, Joan Costa, Andrea Schmitt, Anja Schneider, Howard Cabral, Ozge Cagsal-Getkin, Charles R Vanderburg, Ivana Delalle

“…Multiple statistical analyses of microarray data suggested that certain exosomal miRNAs were differentially expressed in SZ and BD subjects in comparison to controls. …”
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Inferring pathway activity toward precise disease classification. by Eunjung Lee, Han-Yu Chuang, Jong-Won Kim, Trey Ideker, Doheon Lee

“…The advent of microarray technology has made it possible to classify disease states based on gene expression profiles of patients. …”
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Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation. by Adrián Sanz-Moreno, David Fuhrmann, Elmar Wolf, Björn von Eyss, Martin Eilers, Hans-Peter Elsässer

“…Conditional knockout of the Miz1 POZ domain in luminal cells during pregnancy caused a lactation defect with a transient reduction of glandular tissue, reduced proliferation and attenuated differentiation. …”
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MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis by Yi Xie, Congyu Xue, Shuai Guo, Lei Yang

“…Differentially expressed miRNAs between tumor tissues and normal tissues were screened using microarrays, and miR-520a was screened to be significantly poorly expressed in tumor samples. …”
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Caveolin-1 in Tumour Cells is Predominately Expressed in the Basal Subtype of Triple-Negative Breast Carcinoma by Savelina Popovska, Ivan Ivanov, Akishi Ooi

“…Triple-negative breast cancer (TNBC) is a subtype of breast cancer which is clinically negative for expression of steroid hormones and HER2 protein. According to DNA microarray profiling, breast cancer was divided into several molecular subgroups, including Luminal A and Luminal B; HER2 overexpressing; normal breast- like and basal-like subtype. …”
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Immunohistochemistry guided segmentation of benign epithelial cells, in situ lesions, and invasive epithelial cells in breast cancer slides. by Maren Høibø, André Pedersen, Vibeke Grotnes Dale, Sissel Marie Berget, Borgny Ytterhus, Cecilia Lindskog, Elisabeth Wik, Lars A Akslen, Ingerid Reinertsen, Erik Smistad, Marit Valla

“…The sections were derived from four breast cancer cohorts. Tissue microarray cores from a fifth cohort of 21 patients was used as a second test set. …”
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Stabilization of Transcription Factor, HIF-1α by Prolylhydroxylase 1 Knockout Reduces Cardiac Injury After Myocardial Infarction in Mice by Mahesh Thirunavukkarasu, Seetur R. Pradeep, Babatunde Oriowo, Sue Ting Lim, Monica Maloney, Shayan Ahmed, Nicole Taylor, David M. Russell, Pavayee Socrates, Ethan Batko, Matan Berkovsky, John Alexander Palesty, Nilanjana Maulik

“…For microarray analysis, WTMI and PHD1^−/−MI group mices’ heart tissue was used for RNA isolation, then hybridization to a GeneChip™ Mouse Gene 1.0 ST Array as per the manufacturer’s instructions. …”
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