Silencing NEDD4L Effectively Inhibits the Malignant Behaviors of Hepatocellular Carcinoma by Dai J, Zhu T, Wan J, Liu R, Song Y, Zhang D, Wang X, Zhang H, Li W, Lin J, Zhu X, Liu Y

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Transcriptome and Proteome Research in Veterinary Science: What Is Possible and What Questions Can Be Asked? by Robert Klopfleisch, Achim D. Gruber

“…This review gives an overview on the methods currently available for transcriptome analysis, that is, microarrays, Ref-Seq, quantitative PCR arrays and discusses their potentials and limitations. …”
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Increased secretory leukocyte protease inhibitor (SLPI) production by highly metastatic mouse breast cancer cells. by Kevin T Sayers, Alan D Brooks, Thomas J Sayers, Oleg Chertov

“…The precise molecular mechanisms enabling cancer cells to metastasize from the primary tumor to different tissue locations are still largely unknown. Secretion of some proteins by metastatic cells could facilitate metastasis formation. …”
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Comprehensive analysis of single-cell and bulk RNA sequencing data unveils antigen-presenting and processing fibroblasts and establishes a predictive model in gastric cancer by Chenggang Zhang, Fangqi Chen, Jie Li, Yixuan He, Juan Sun, Zicheng Zheng, Guanmo Liu, Yihua Wang, Weiming Kang, Xin Ye

“…Immunohistochemistry of GC tissue microarrays was performed to validate the model. …”
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Comprehensive Expression Profiling and Molecular Basis of CDC28 Protein Kinase Regulatory Subunit 2 in Cervical Cancer by Li Qin, Xiaoqiong Luo, Xiao Qin, Hongbao Huang, Lianling Zhang, Shengcai Chen, Xiaoqin Wu, Bingsheng Huang, Jian Pan, Jingxi Wei

“…Herein, taking advantage of massive genetics data from multicenter RNA-seq and microarrays, we were the first group to perform tissue microarrays for CKS2 in cervical cancer. …”
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Low density of intratumoral M1-macrophage infiltration may correlate with worse prognosis in low-grade early-stage uterine endometrioid carcinoma by Michiko Nagamine, Hiroshi Ogi, Maki Hirai, Ikoi Omatsu, Sanzo Moriwaki, Saya Shibata, Satoru Yasukawa, Kaori Yoriki, Motohiro Kojima, Taisuke Mori, Kyoko Itoh, Eiichi Konishi

“…Multiplex immunohistochemistry was performed on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue blocks of primary surgical specimens and, when available, recurrent lesions. …”
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ZNF37A downregulation promotes TNFRSF6B expression and leads to therapeutic resistance to concurrent chemoradiotherapy in rectal cancer patients by Ying Huang, Jing Jin, Ningxin Ren, Hongxia Chen, Yan Qiao, Shuangmei Zou, Xin Wang, Linlin Zheng, Ye-Xiong Li, Wen Tan, Dongxin Lin

“…The genome-wide expression profiling microarray were performed in the tissues of 81 rectal cancer patients before neoadjuvant therapy to analyze and discover DEGs related to chemoradiotherapy resistance, and the results were verified in 45 rectal cancer patients, and finally a 20-gene signature was proposed to be a predictor of chemoradiotherapy response. …”
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Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling by Jiachen Dong, Jiachen Dong, Jiachen Dong, Yue Liao, Yue Liao, Yue Liao, Mengjun Sun, Mengjun Sun, Mengjun Sun, Huiwen Chen, Huiwen Chen, Huiwen Chen, Kecong Zhou, Kecong Zhou, Kecong Zhou, Huanyu Zhang, Huanyu Zhang, Huanyu Zhang, Wei Zhou, Wei Zhou, Wei Zhou, Zhongchen Song, Zhongchen Song, Zhongchen Song

“…The effects of gingipains on bone marrow mesenchymal stem cells (BMSCs) and RAW264.7 macrophages were assessed using cell proliferation assays, qPCR, western blot, microarray analysis, and dual-luciferase reporter assays. …”
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Global gene expression analysis of murine limb development. by Leila Taher, Nicole M Collette, Deepa Murugesh, Evan Maxwell, Ivan Ovcharenko, Gabriela G Loots

“…We used whole-genome microarrays to identify genes with differential expression at 5 stages of limb development (E9.5 to 13.5), during fore- and hind-limb patterning. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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Multiplex imaging reveals novel patterns of MRTFA/B activation in the breast cancer microenvironment by Stephanie M. Wilk, Kihak Lee, Caitlyn C. Castillo, Mohamed Haloul, Alexa M. Gajda, Virgilia Macias, Elizabeth L. Wiley, Zhengjia Chen, Xinyi Liu, Xiaowei Wang, Maria Sverdlov, Kent F. Hoskins, Ekrem Emrah Er

“…Methods We used a multiplexed immunofluorescence approach to label MRTFA, MRTFB, tumor cells by using pan Cytokeratin, endothelial cells by using CD31, and antigen presenting cells (APCs) by using HLA-DRA on two different breast cancer tissue microarrays (TMA): The breast cancer progression TMA provided by the Cooperative Human Tissue Network (CHTN_BrCaProg3) and the University of Illinois Breast Cancer Working Group (TMA BCWG UIC-001-TMA) that included primary tumor and lymph node metastases from patients residing in the West Side and South Side of Chicago. …”
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Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status by Yan Zheng, Baichen Sun, Zhiling Qu

“…This study investigates the expression and prognostic value of ASPM, focusing on its role in chemotherapy outcomes.Methods We analyzed the prognostic relevance of ASPM using bioinformatics, immunohistochemical staining of LUAD tissue microarrays, and proteomics data. Further, in vitro experiments were conducted to evaluate the effects of ASPM overexpression on cell proliferation and sensitivity to cisplatin.Results Bioinformatics analysis revealed that ASPM’s prognostic significance differed between transcriptomic and proteomic datasets. …”
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Comprehensive analysis of the clinical and biological significances of polyamine metabolism in glioma by Fei Bai, Kexin Zhai, Tiantian Yao, Yuxia Zhou, Yongxiang Zhao, Guanghui Yang

“…What’s more, IHC staining was conducted on glioma tissue microarrays to confirm the expression patterns of genes related to polyamine metabolism in glioma tissues. …”
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Development and In Vitro Characterization of [³H]GMC-058 as Radioligand for Imaging Parkinsonian-Related Proteinopathies by Andrea Varrone, Vasco C. Sousa, Manolo Mugnaini, Sandra Biesinger, Gunnar Nordvall, Lee Kingston, Ileana Guzzetti, Charles S. Elmore, Dan Sunnemark, Dinahlee Saturnino Guarino, Sjoerd J. Finnema, Magnus Schou

“…[³H]GMC-058 was the only compound with negligible binding in controls, and was further evaluated using tissue microarrays, autoradiography on fresh-frozen brain slices, and in vitro saturation binding assay on brain homogenates. …”
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Decoding the prognostic landscape of LUAD: the interplay between N6-methyladenosine modification and immune microenvironment by Quan Chen, Quan Chen, Weijun Wan, Qing Zhao, Juan Li, Juan Li, Yanli Xiong, Yanli Xiong, Yuchuan Yuan, Lu Tang, Xiaofeng Wu, Wei Xing, Wei Guo, Di Lu, Luoquan Ao, Xiang Xu, Xiang Xu, Xiang Xu, Xiang Ao, Xiang Ao, Xiang Ao

“…These findings were validated by two independent databases and LUAD tissue microarrays. METTL3 was found to impact the mRNA expression of these genes, with METTL3 deficiency abolishing these interactions. …”
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Association of PD-1, LAG-3 and TIM-3 expression on intratumoral CD8 T-cells with response to atezolizumab in a Real-World-Evidence biomarker study for advanced urothelial carcinoma... by Carlos E. de Andrea, Marta Abengozar-Muela, José Ángel Arranz, Miguel Angel Climent, Javier Puente, Ángel Vizcay, Ligia Montero de la Fuente, José Miguel Jurado, Teresa Bonfill, Carmen Santander, José Carlos Villa, Eduardo Pujol, Adriana Carolina Rosero, Jenifer Gomez, Eva María Fernández, Carlos Álvarez Fernández, Ignacio Ramirez, Pablo Arnáiz, Álvaro López-Janeiro, Ignacio Melero, Miguel F. Sanmamed, José Luis Pérez-Gracia

“…We assessed with Multiplexed Quantitative Immunofluorescence the expression of CD8, PD-1, TIM-3 and LAG-3 on T-cells in the different compartments of the TME (tumor, stroma and whole tissue) in pre-treatment tissue microarrays. We studied associations between the expression of the markers and clinical efficacy. …”
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Tumor-infiltrating B cells produce tumor-specific antibodies and may contribute to suppressing tumor in head and neck squamous cell carcinoma by Junsei Sameshima, Hu Chen, Naoki Kaneko, Lijing Yan, Shiho Yokomizo, Tomoki Sueyoshi, Haruki Nagano, Taiki Sakamoto, Shoichi Tanaka, Yasuyuki Maruse, Taichi Hattori, Ryoji Kitamura, Yoshikazu Hayashi, Takashi Maehara, Shinsuke Fujii, Tamotsu Kiyoshima, Thomas Guy, Zivile Giedraityte, Wataru Kumamaru, Masafumi Moriyama, Shintaro Kawano

“…These ASCs exhibited a low somatic hypermutation frequency, indicating that they are likely activated through extrafollicular response rather than germinal center response. Protein microarrays with recombinant antibodies mimicking TIL-Bs secretory antibodies and serum antibodies revealed several antigens highly expressed in tumor cells. …”
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Gene Expression Analysis and Validation of a Novel Biomarker Signature for Early-Stage Lung Adenocarcinoma by Sanjan S. Sarang, Catherine M. Cahill, Jack T. Rogers

“…This study aimed to identify a novel biomarker signature of early-stage LUAD utilizing gene expression analysis of human lung tissue samples. Using 22 pairs of LUAD and matched normal lung microarrays, 229 differentially expressed genes were identified. …”
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The expression and prognosis for Aurora kinases in human non-small cell lung cancer by Yue Wang, Juan Liu, Jiaxue Xu, Zhaodong Ji

“…Immunohistochemistry (IHC) was performed on tissue microarrays (TMAs) from 29 ‌lung adenocarcinoma (LUAD) patients. …”
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Expression profiling the temperature-dependent amphibian response to infection by Batrachochytrium dendrobatidis. by Laia Ribas, Ming-Shi Li, Benjamin J Doddington, Jacques Robert, Judith A Seidel, J Simon Kroll, Lyle B Zimmerman, Nicholas C Grassly, Trenton W J Garner, Matthew C Fisher

“…Using temporal expression-profiling by microarrays and qRT-PCR, we characterise this response in the main amphibian lymphoid tissue, the spleen. …”
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