Prognostic value of B7-H3 expression in metastatic renal cell carcinoma and its impact on immunotherapy response by Faruk Recep Özalp, Kutsal Yörükoğlu, Eda Çalışkan Yıldırım, Mehmet Uzun, Hüseyin Salih Semiz

“…Methods Immunohistochemical analysis of B7-H3 expression was performed in 84 metastatic RCC patients. Tissue microarrays and separate sections of formalin-fixed paraffin-embedded tissue were used for immunohistochemical staining. …”
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Impact of MiRNAs on Wnt-related gene activity in breast cancer by Tomasz Sirek, Agata Sirek, Nikola Zmarzły, Marcin Opławski, Katarzyna Król-Jatręga, Dariusz Boroń, Michał Chalcarz, Piotr Ossowski, Konrad Dziobek, Damian Strojny, Joanna Szymańska, Julia Gajdeczka, Przemysław Borawski, Kacper Boroń, Beniamin Oskar Grabarek

“…Expression profile of Wnt-related genes was assessed with mRNA microarrays and reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
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Public Database-based Study to Explore the Expression and Role of DDB1 
in Lung Adenocarcinoma by Xinkai ZOU, Ziyi HE, Yanfei ZHANG, Yi HU, Xiaomin WANG, Zhongjie WU

“…Single cell analysis showed that DDB1 was mainly expressed in T cells, alveolar macrophages and dendritic cells. Tissue microarrays confirmed that overall survival was shorter in the DDB1 high expression group (P<0.001), and Cox multifactorial analysis showed that DDB1 was an independent predictor of LUAD prognosis. …”
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Dual-targeting and steric hindrance resolution in HER2 IHC: a novel approach to improve diagnostic sensitivity by Li Luo, Xi Zhang, Linqiong Chen, Zhuohan Chen, Yuchen Wang, Kaihao Huang, Xiaoyun Lin, Hongxiang Zhu, Wangqi Du

“…We assessed the detection performance of Nby-Aby in BC tissues and compared it with conventional HER2 diagnostic antibodies using tissue microarrays (TMAs). …”
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Quantifying intratumoral biomarker heterogeneity in tubo-ovarian high-grade serous carcinoma to optimize clinical translation by Aline Talhouk, Derek S. Chiu, Liliane Meunier, Kurosh Rahimi, Cécile Le Page, Monique Bernard, Diane Provencher, David G. Huntsman, Anne Marie Mes Masson, Martin Köbel

“…Markers were stained on tissue microarrays and scored using a continuous or categorical interpretation of staining patterns. …”
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Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway by Yang Sheng Wu, Zhe Ming Li, Yi Tao Chen, Shi Jie Dai, Xiao Jie Zhou, Yuan Xiao Yang, Jian Shu Lou, Li Ting Ji, Yu Ting Bao, Ling Xuan, Lu Ning Lin, Chang Yu Li

“…Biochemical measurements and liver histopathology were detected using the biochemical analyzer and HE staining in ZDF rats, respectively. Microarray analysis of liver tissues was performed, and differentially expressed gene (DEG) levels were examined by quantitative real-time PCR (qPCR) and Western blot. …”
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Toll-like receptor 2 and Toll-like receptor 4 predict favorable prognosis in local pancreatic cancer by Mira A Lanki, Hanna E Seppänen, Harri K Mustonen, Camilla Böckelman, Anne T Juuti, Jaana K Hagström, Caj H Haglund

“…Patients who received neoadjuvant therapy were excluded. Tissue microarrays and immunohistochemistry allowed assessment of the expression of Toll-like receptor 2 and Toll-like receptor 4 in pancreatic ductal adenocarcinoma tissue, and we matched staining results against clinicopathological parameters using Fisher’s test. …”
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IL-6 is a prognostic biomarker in patients with advanced esophageal squamous cell carcinoma received with PD-1 inhibitors by Ping Huang, Manyi Zhao, Jianhong Xia, Hongliang Li, Junxia Sun, Xin Li, Chunsheng Yang, Guangyi Gao, Wenhang Zhou, Meifeng Zhong, Hongmei Yong

“…CD8, IL-6, and PD-L1 expression in ESCC tissue microarrays were measured using immunohistochemistry, and then the tumor microenvironment was classified. …”
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Inflammatory markers after supplementation with marine n-3 or plant n-6 PUFAs: A randomized double-blind crossover study by Elise Grytten, Johnny Laupsa-Borge, Kaya Cetin, Pavol Bohov, Jan Erik Nordrehaug, Jon Skorve, Rolf K. Berge, Elin Strand, Bodil Bjørndal, Ottar K. Nygård, Espen Rostrup, Gunnar Mellgren, Simon N. Dankel

“…Cytokines and chemokines (multiplex assay), acute-phase proteins (MALDI-TOF mass spectrometry), endothelial function (vascular reaction index), blood pressure, FA composition (red blood cell membranes/serum/adipose tissue, GC-MS/MS), and adipose gene expression (microarrays, quantitative PCR) were measured. …”
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Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study by Bernard A Fox, Chichung Wang, Kristin Roman, Carla Coltharp, Carmen Ballesteros-Merino, Shawn M Jensen, Janis M Taube, David L Rimm, Cliff Hoyt, Mei Jiang, Edwin R Parra, Ignacio Wistuba, Elizabeth L Engle, John McGuire, Bethany Remeniuk, Darren Locke

“…Serial sections from tonsil and breast carcinoma and non-small cell lung cancer (NSCLC) tissue microarrays (TMAs), TSA-Opal fluorescent detection reagents, and antibodies were distributed to the six sites equipped with a Leica Bond Rx autostainer and a Vectra Polaris multispectral imaging platform. …”
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SFB flagellin mediates cell adhesion, endocytosis and immune regulation in germ-free mice by Huahai Chen, Huahai Chen, Huahai Chen, Huahai Chen, Huahai Chen, Liu Wu, Liu Wu, Xiongyu Cao, Xiongyu Cao, Zongyan Li, Zongyan Li, Renjun Zhu, Renjun Zhu, Xiaojing Wang, Jun Li, Jun Li, Jun Li, Zuzhang Wei, Zuzhang Wei, Dengfeng Yang, Dengfeng Yang, Dengfeng Yang, Yeshi Yin, Yeshi Yin

“…Immunohistochemical analysis of ileal tissues showed co-localization of recombinant bacteria with the lysosomal-associated membrane protein 2 (Lamp2), confirming cellular internalization. …”
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Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC by Yulin Zhang, Yixu Wang, Jilin Peng, Kun Zhao, Ling Li, Yuan Zhang, Ziyu Zhai, Sijie Yuan, Shichao Li, Shichao Li, Shichao Li, Fanglei Ye, Le Wang

“…Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. …”
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α5-nAChR/NETO2 contributed to chronic stress-promoted lung adenocarcinoma progression by Jingting Wang, Jiaying Cai, Zengping Wang, Shuran Yang, Jing Wang, Yanfei Jia, Haiji Sun, Xiaoli Ma

“…Results α5-nAChR expression was related to NETO2 expression, low survival rate, staging as well as smoking status in LUAD dataset as well as tissue microarrays. The correlation between α5-nAChR and NETO2 was validated in nude mice xenograft tissues. α5-nAChR as well as NETO2 expression correlated in CRS and CUMS mice tissues. …”
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In vivo RNAi screen and validation reveals Ngp, Hba-a1, and S100a8 as novel inhibitory targets on T lymphocytes in liver cancer by Inga Hochnadel, Lisa Hoenicke, Nataliia Petriv, Huizhen Suo, Lothar Groebe, Chantal Olijnik, Nina Bondarenko, Nina Bondarenko, Juan C. Alfonso, Michael Jarek, Ruibing Shi, Andreas Jeron, Andreas Jeron, Kai Timrott, Tatjana Hirsch, Nils Jedicke, Dunja Bruder, Dunja Bruder, Frank Klawonn, Frank Klawonn, Frank Klawonn, Ralf Lichtinghagen, Robert Geffers, Henrike Lenzen, Henrike Lenzen, Michael P. Manns, Tetyana Yevsa

“…Therefore, in this study, we aimed to define novel ICI molecules arising on T cells during aggressive HCC development.MethodsUsing autochthonous HCC models, we performed microarray analyses followed by in vivo RNA interference screen and identified several new ICI molecules on CD4 and CD8 T lymphocytes in HCC-bearing mice. …”
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CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling by Zihao Wei, Ying Wang, Jiakuan Peng, Honglin Li, Junjie Gu, Ning Ji, Taiwei Li, Xikun Zhou, Xin Zeng, Jing Li, Qianming Chen

“…Here, we demonstrated that the circRFWD3 was significantly upregulated in HNSCC tissues and cell lines by circRNA microarray analysis and qPCR. …”
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Evaluation of Organoid‐Derived Exosomal microRNA as Liquid Biopsy for Colorectal Cancer: A Multicenter Cross‐Sectional Study by Atsuhiro Sasaki, Masatake Kuroha, Masaki Tosa, Seiichi Takahashi, Shinya Oomori, Eiki Nomura, Tatsuya Kikuchi, Motoyuki Onodera, Yuichiro Sato, Teruko Miyazawa, Hirofumi Chiba, Naonobu Yokoyama, Jun Kusaka, Daisuke Okamoto, Tomoyuki Handa, Mikako Sugimura, Masahiro Iwabuchi, Keiichiro Hiramoto, Hidekazu Shirota, Hideya Iwaki, Hiroshi Nagai, Yusuke Shimoyama, Takeo Naito, Rintaro Moroi, Hisashi Shiga, Yoichi Kakuta, Yoshitaka Kinouchi, Atsushi Masamune

“…Organoids were established from normal colon and colorectal cancer tissues. Exosomes were isolated from conditioned media. miRNAs were extracted from exosomes and compared using microarray analysis. …”
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MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
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Frequent PD-L1 expression in oral squamous cell carcinoma of non-smokers and non-drinkers, and association of tumor infiltrating lymphocytes with favorable prognosis. by FJ Mulder, EJ de Ruiter, TFB Gielgens, F Farshadpour, R de Bree, MFCM van den Hout, B Kremer, SM Willems, EJM Speel

“…Materials and methods: Clinical characteristics and tumor tissue of 86 NSND with OSCC were retrospectively collected and analyzed for protein expression on tissue microarrays. …”
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Prognostic value of Osteopontin (SPP1) in colorectal carcinoma requires a personalized molecular approach by Mourad Assidi, Wafaey Gomaa, Mohammad Jafri, Mehenaz Hanbazazh, Mahmoud Al-Ahwal, Peter Pushparaj, Asia Al-Harbi, Mohammed Al-Qahtani, Abdelbaset Buhmeida, Jaudah Al-Maghrabi

“…Hundred thirty-four (134) archival paraffin blocks of colorectal cancer were collected from King Abdulaziz University Hospital, Saudi Arabia. Tissue microarrays were constructed, and automated immunohistochemistry was performed to evaluate SPP1 protein expression patterns in colorectal cancer. …”
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CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer by Xiao Ma, Baojian Zhang, Xuezhe Yin, ShiPeng Yang, Zhenhua Lin, Yang Yang, Xianchun Zhou

“…Finally, the prognostic significance of CPT1A and the vascular marker VEGF in BC tissues was evaluated using tissue microarrays and public databases. …”
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