Cancer-associated fibroblast-derived fibulin-5 promotes epithelial–mesenchymal transition in diffuse-type gastric cancer via cAMP response element-binding protein pathway, showing... by Jinju Choi, Yoonjin Kwak, Miree Park, Jeong Yeon Jo, Jun Hyuk Kang, Kook Myeong-Cherl, Hang-Rae Kim, Gwanghun Kim, Seong-Ho Kong, Do-Joong Park, Hye Seung Lee, Hyuk-Joon Lee, Jung Mogg Kim, Sang Gyun Kim, Han-Kwang Yang, Ji Kon Ryu, Soo-Jeong Cho

“…For validation, we used tissue microarray blocks of gastric cancers with immunohistochemical staining, single-cell RNA sequencing, primary cultures of cancer-associated fibroblasts (CAFs) and organoids, and a co-culture system involving CAFs and cancer cells. …”
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Repurposing MDM2 inhibitor RG7388 for TP53-mutant NSCLC: a p53-independent pyroptotic mechanism via ROS/p-p38/NOXA/caspase-3/GSDME axis by Gaoyan Tang, Xuelei Cao, Jiaqi Chen, Fu Hui, Na Xu, Yiqing Jiang, Hongmin Lu, Haifeng Xiao, Xiuming Liang, Mingzhe Ma, Yu Qian, Dongli Liu, Zhenlu Wang, Shuzhen Liu, Guohua Yu, Lei Sun

“…Furthermore, immunohistochemical (IHC) analysis of an NSCLC tissue microarray confirmed a strong positive correlation between p-p38 and NOXA expression. …”
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Spatial transcriptomics and multi-omics reveal relapse and resistance mechanisms of EndMT-derived CAFs mediated by TNC and FLNC in glioblastoma by Li Ji, Die Xia, Yu Zhou, Yaling Hu, Zhenkun Yang, Ying Yin, Jingjing Wang, Bo Zhang, Lingli Gong, Koukou Li, Jian Zou, Mei Wang

“…Methods Spatial transcriptome (ST) sequencing was conducted on the tissue microarray encompassing primary and recurrent glioma samples in order to characterize the cellular composition. …”
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USP7 promotes temozolomide resistance by stabilizing MGMT in glioblastoma by Jiabing Li, Xiaorong Feng, Zhaohui Liu, Yunfang Deng, Zhiming Sun, Bei Chen, Lihui Wu, Xiaolong Wang, Lin Miao, Liyuan Zeng, Lei Hu, Yuming He, Ying Sheng, Yue Liu, Yu Zhao

“…Inhibition or knockdown of USP7 reduces MGMT levels, as well as those of XPC, ALKBH2, and ALKBH3, impairs DNA repair capacity, and sensitizes GBM cells to TMZ, enabling effective treatment with reduced TMZ dosages. Clinically, tissue microarray analyses reveal that USP7 and MGMT co-overexpression in GBM correlates with poor patient survival. …”
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NF1 expression profiling in IDH-wildtype glioblastoma: genomic associations and survival outcomes by Michael Chang, Mohamed Sherief, Maria Ioannou, Viveka Chinnasamy, Lucy Chen, Michael Frost, Michelle Mattson-Hoss, Herb Sarnoff, David O. Kamson, Matthias Holdhoff, Debraj Mukherjee, Chetan Bettegowda, Jordina Rincon-Torroella, Victoria Croog, Peng Huang, Fausto J. Rodriguez, Calixto-Hope G. Lucas, Karisa C. Schreck

“…We selected 69 GBMs from which to build a tissue microarray (TMA) of 44 NF1-wildtype and 25 NF1-mutant cases. …”
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MET overexpression contributes to STAT4-PD-L1 signaling activation associated with tumor-associated, macrophages-mediated immunosuppression in primary glioblastomas by Ying Zhang, Zheng Wang, Zheng Zhao, Tao Jiang, Shou-Wei Li, Yong-Jian Zhu, Qiang-Wei Wang, Li-Hua Sun, Zhi-Liang Wang, Kuan-Yu Wang, Guan-Zhang Li, Jian-Bao Xu, Chang-Yuan Ren, Wen-Ping Ma, Hong-Jun Wang, Zhao-Shi Bao

“…Western blot analysis revealed that in GBM cell lines (N33 and LN229), PD-L1 and phosphorylated STAT4 were upregulated by MET activation treatment with hepatocyte growth factor and were downregulated on MET suppression by PLB-1001. Tumor tissue microarray analysis indicated a positive correlation between MET and PD-L1 and macrophage-associated markers. …”
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Differential expression pattern of CC chemokine receptor 7 guides precision treatment of hepatocellular carcinoma by Jie Qin, Qianyi Gong, Cheng Zhou, Jietian Xu, Yifei Cheng, Weiyue Xu, Di Zhu, Yiming Liu, Yuye Zhang, Yanru Wang, Lingling Gao, Lanfang Li, Wulei Hou, Qian Li, Binbin Liu, Yazhen Zhu, Zuoyun Wang, Jieyi Shi, Shuangjian Qiu, Chunmin Liang

“…In this study, the complex tumor microenvironment was identified by using tissue microarray (TMA), spatial transcriptomes and single-cell sequencing. …”
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The independent prognostic impact of the GATA2 pioneering factor is restricted to ERG-negative prostate cancer by Franziska Büscheck, Maciej Zub, Asmus Heumann, Claudia Hube-Magg, Ronald Simon, Dagmar S Lang, Doris Höflmayer, Emily Neubauer, Frank Jacobsen, Andrea Hinsch, Andreas M Luebke, Maria Christina Tsourlakis, Guido Sauter, Hartwig Huland, Markus Graefen, Alexander Haese, Hans Heinzer, Torsten Schlomm, Till S Clauditz, Eike Burandt, Waldemar Wilczak, Stefan Steurer, Sarah Minner

“…To understand the prognostic potential of GATA2 assessment in prostate cancer, we analyzed nuclear GATA2 expression on an annotated tissue microarray with 12,427 prostate cancer samples. …”
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Aldo-keto reductase family 1 member B10 prevents esophageal squamous cell carcinoma from reactive carbonyl species-induced cell death and promotes its progression by Shau-Hsuan Li, Wan-Ting Huang, Yen-Hao Chen, Hung-I Lu, Chien-Ming Lo, Hsin-Ting Tsai, Chang-Han Chen

“…Methods To identify significant gene clusters involved in the metabolism of RCS in ESCC, we used an Agilent SurePrint G3 Human V2 GE 8 × 60 K microarray kit to analyze differentially expressed genes between nine paired ESCC tissues and adjacent normal esophageal tissues taken from areas distant from the tumor site. …”
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Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy by Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du

“…Methods The expression of CD155 was analyzed across various cancer types using data from The Cancer Genome Atlas (TCGA) and validated by tissue microarray analysis. The impact of CD155 on T cell mediated cytotoxicity was analyzed using CD155 over-expression or knockout tumor cells. …”
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Homogeneity in immune features between colorectal liver metastases better identifies patients with good prognosis compared to pathological response to preoperative chemotherapy by David Henault, David Stephen, Pierre-Antoine St-Hilaire, Nouredin Messaoudi, Franck Vandenbroucke-Menu, Eve Simoneau, Zhixia Rong, Marylène Plasse, Richard Létourneau, André Roy, Michel Dagenais, Réal Lapointe, Bich Nguyen, Anne-Marie Mes-Masson, G. Soucy, Simon Turcotte

“…However, the prognostic significance of the heterogeneity of these features in patients with multiple CRLMs remains under investigation. We used a tissue microarray of 220 mismatch repair-gene proficient CRLMs resected in 97 patients followed prospectively to quantify CD3+ T cells and MHC-I by immunohistochemistry. …”
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Reprogramming‐derived gene cocktail increases cardiomyocyte proliferation for heart regeneration by Yuan‐Yuan Cheng, Yu‐Ting Yan, David J Lundy, Annie HA Lo, Yu‐Ping Wang, Shu‐Chian Ruan, Po‐Ju Lin, Patrick CH Hsieh

“…In this study, we identified a distinct stage within the initiation phase of CM reprogramming before the MET process, and microarray analysis revealed the strong up‐regulation of several mitosis‐related genes at this stage of reprogramming. …”
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HspB5 correlates with poor prognosis in colorectal cancer and prompts epithelial-mesenchymal transition through ERK signaling. by Qinghua Li, Yanlan Wang, Yuexing Lai, Ping Xu, Zhiwen Yang

“…Alpha B-crystallin (HspB5) is abnormally expressed in tumor tissues and portends a poor prognosis in cancer patients. …”
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Theory on the coupled stochastic dynamics of transcription and splice-site recognition. by Rajamanickam Murugan, Gabriel Kreiman

“…We evaluate these theoretical predictions by considering human and mouse exon microarray data as well as RNAseq data from multiple different tissues. …”
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Characteristic expression profile of small nucleolar RNAs and their association with tumor immune microenvironment in papillary thyroid carcinoma by Qi-Lan Jiang, Fu-Yi Wang, Yuan Men, Yi-Huan Wu, Qin Xu, Yang Zeng, Jun Jiang

“…In this study, we employed small RNA microarray analysis to profile snoRNA expression in tumor tissues and corresponding normal thyroid tissues obtained from patients undergoing thyroidectomy. …”
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Neutrophil Extracellular Traps Drive Kidney Stone Formation by Zhiming Yang, Xiong Chen, Guannan Qi, Jie Gu, Zheng Liu, Xiaobo Zhang

“…MPO, H3Cit, and NE in kidney tissues were detected via immunofluorescence staining. …”
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Integrated Bioinformatics Analysis of Hub Genes and Pathways in Anaplastic Thyroid Carcinomas by Xueren Gao, Jianguo Wang, Shulong Zhang

“…The differentially expressed genes (DEGs) between ATC tissues and normal thyroid tissues were screened out by the limma package and then enriched by gene ontology (GO) and KEGG pathway analysis. …”
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Serum CXCL13 as a Novel Biomarker in Oral Squamous Cell Carcinoma by Shin Tojo, Koh‐ichi Nakashiro, Nobuyuki Kuribayashi, Daisuke Uchida

“…Methods Initially, we performed microarray analysis to evaluate the gene expression signatures of primary OSCC and normal oral mucosal tissues. …”
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c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma by Jing Feng, Wei He, Jin Fang

“…Differential expression of LAMP3 in TSCC from GEO microarray was analyzed, and pathway enrichment analysis of LAMP3 was performed utilizing GSEA. …”
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Transcriptomic characterization of temperature stress responses in larval zebrafish. by Yong Long, Linchun Li, Qing Li, Xiaozhen He, Zongbin Cui

“…Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. …”
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