RBIS regulates ribosome biogenesis to affect progression in lung adenocarcinoma by Hongyu Pan, Li Liao, Siwei Xu, Yujian Xu, Wenjun Chai, Xiaoli Liu, Jing Li, Yue Cao, Lei Sun, Qian Liu, Mingxia Yan

“…Additionally, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was utilized to quantify the mRNA expression levels of genes. …”
Get full text

The Analysis of Driving Factors of Farm Land Equipment to Sprinkler Irrigation Systems (Case Study: the Villages of Ravansar Township) by Jafar Tavakoli, Davoud Jamini

“…Prioritized in order of importance, the recognized factors and their variances are: supportive policies (19.168), strong educational-promotive system (17.308), active participation of farmers (13.459), reliability and efficiency of agricultural land (12.181) and contextual factors (10.893).…”
Get full text

Optimization Function for Determining Optimal Dose Range for Beef and Seed Potato Irradiation by Elena Kozlova, Ulyana Bliznyuk, Alexander Chernyaev, Polina Borshchegovskaya, Arcady Braun, Victoria Ipatova, Sergey Zolotov, Alexander Nikitchenko, Natalya Chulikova, Anna Malyuga, Yana Zubritskaya, Timofey Bolotnik, Anastasia Oprunenko, Aleksandr Kozlov, Mikhail Beklemishev, Roza Yagudina, Igor Rodin

“…The use of proprietary methods involving spectrophotometry and high-performance liquid chromatography–mass spectrometry provides a novel perspective on the quantitative assessment of the myoglobin oxidation level and the potential damage to albumin’s native structure.…”
Get full text

Inhibition of HPV18 E6/E7 Protein-Expressing HeLa Cell Proliferation Using Optimized De Novo CRISPR/Cas9 Constructs Delivered by the LL-37 Peptide by Niloofar Khairkhah1, Azam Bolhassani2, 3*, Reza Najafipour1, 4, Ali Namvar3, Alireza Milani2, Elnaz Agi3, Ali Anvar3, Mohammad Sadeqh Khosravy5

“…LL-37 peptide demonstrated efficient delivery of CRISPR/Cas9 vectors into HeLa tumor cells, with an optimal nitrogen-to-phosphate (N/P) ratio of 5: 1, achieving high transfection efficiency without systemic toxicity. …”
Get full text

Evaluation of the Effect of Floating Treatment Wetlands Planted with <i>Sesuvium portulacastrum</i> on the Dynamics of Dissolved Inorganic Nitrogen, CO₂, and N<sub>2</su... by Shenghua Zheng, Man Wu, Jian Liu, Wangwang Ye, Yongqing Lin, Miaofeng Yang, Huidong Zheng, Fang Yang, Donglian Luo, Liyang Zhan

“…Higher primary production may be the reason that the DIC concentration was lower in the plant group than in the control group, whereas efficient nitrification and uptake by plants reduced the availability of NH₄⁺ in the plant group, thereby reducing the production of N₂O. …”
Get full text

Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinoma by Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, Gehong Dong, Yong Zhang, Yinshan Li, Liang Cheng, Weimin Ci, Wenqiang Yu, Chuanliang Xu

“…Based on the observation that PCDHGB7 was hypermethylated in UC, we developed a bisulfite‐free quantitative polymerase chain reaction (qPCR)‐based PCDHGB7 evaluation to enable urine for UC noninvasive detection. …”
Get full text

Single-cell eQTL mapping in yeast reveals a tradeoff between growth and reproduction by James Boocock, Noah Alexander, Leslie Alamo Tapia, Laura Walter-McNeill, Shivani Prashant Patel, Chetan Munugala, Joshua S Bloom, Leonid Kruglyak

“…Expression quantitative trait loci (eQTLs) provide a key bridge between noncoding DNA sequence variants and organismal traits. …”
Get full text

Giardiavirus infection alleviates growth restriction and intestinal damage caused by the intestinal parasite Giardia duodenalis by Lu Li, Lili Cao, Qiankun Yang, Zhiteng Zhao, Jianqi Yuan, Shaoxiong Liu, Qinqin Jin, Jianhua Li, Xin Li, Xiaocen Wang, Nan Zhang, Weina Jiang, Pengtao Gong

“…The virulence of Giardiavirus (GLV) may efficiently clear Giardia parasites from infected patients. …”
Get full text

Gammaherpesvirus infection triggers the formation of tRNA fragments from premature tRNAs by Aidan C. Manning, Mahmoud M. Bashir, Kyle Rapchak, Calyssa J. Huff, Ariana R. Jimenez, Sheila Gonzalez, Courtney L. Woodruff, Heather E. Upton, Kathleen Collins, Todd M. Lowe, Jessica M. Tucker

“…Here, we combined two new approaches, sequencing library preparation by ordered two template relay (OTTR) and tRNA bioinformatic analysis by tRAX, to quantitatively profile full-length tRNAs and tRNA fragment (tRF) identities during MHV68 infection. …”
Get full text

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets by Xinjin Liu, Jiangpeng Feng, Qiuhan Zhang, Dong Guo, Lu Zhang, Tao Suo, Wenjia Hu, Ming Guo, Xin Wang, Zhixiang Huang, Yong Xiong, Guozhong Chen, Yu Chen, Ke Lan

“…Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. …”
Get full text

Dynamic recrystallization mechanism of high-strength Mg-Gd-Y-Zn-Mn alloy by hot compression deformation by Yini Lin, Shiming Wu, Yongxin Wang, Hao Chen, Guo Li, Weidong Xie, Guobing Wei, Yan Yang, Xiaodong Peng

“…The results show that at a low strain rate of 0.01 s−1 and 723 K, the grain size significantly decreases compared to 623 K, the grain boundaries protrude outward, and new small grains are generated. Quantitative calculations indicate that the thermal deformation mechanism can be reflected by the dissipation efficiency factor η. …”
Get full text

Genome‐wide association study and BSR‐seq identify nitrate reductase–related genes in rice landraces (Oryza sativa L.) by Shuangshuang Luo, Zuyu Liao, Shilv Huang, Xiuzhong Xia, Zongqiong Zhang, Baoxuan Nong, Tongping Luo, Chenli Zhu, Can Chen, Hui Guo, Rui Feng, Yinghua Pan, Shuhui Liang, Yongcheng Li, Jianhui Liu, Yongfu Qiu, Danting Li, Xinghai Yang

“…However, the lower nitrogen use efficiency (NUE) results in an N fertilizer surplus, which causes many environmental problems. …”
Get full text

An Underground Goaf Locating Framework Based on D-InSAR with Three Different Prior Geological Information Conditions by Kewei Zhang, Yunjia Wang, Feng Zhao, Zhanguo Ma, Guangqian Zou, Teng Wang, Nianbin Zhang, Wenqi Huo, Xinpeng Diao, Dawei Zhou, Zhongwei Shen

“…In order to enhance the operational efficiency and locating accuracy of underground goaf, deformation model selection must be aligned with site-specific geological conditions under varying cases of prior information. …”
Get full text

Threshold Effects of Nitrogen Fertilization Rates on Growth and Essential Oil Yield with Component Regulation in <i>Cinnamomum camphora</i> var. <i>linaloolifera</i> by Zhirong Liu, Xinyi Chen, Jiao Zhao, Luyuan Sun, Jian Guo, Yangyang Shao, Jia Liu, Lei Zhong, Haiyan Zhang, Yanbo Wang, Jie Zhang

“…The findings reveal a threshold response to nitrogen input, wherein rates exceeding 90 kg·hm⁻² did not confer further benefits and may reduce efficiency. Collectively, these results suggest that a nitrogen application rate of 90 kg·hm⁻² optimally enhances vegetative growth, nitrogen assimilation, and both the quantitative and qualitative traits of essential oils in <i>C. camphora</i> var. …”
Get full text

Circulating Baseline CXCR3+Th2 and Th17 Cell Proportions Correlate With Trabecular Bone Loss After 48 Weeks of Biological Treatment in Early Rheumatoid Arthritis by Julia M. Scheffler, Christina Drevinge, Catharina Lindholm, Inger Gjertsson, Kristina Lend, Merete Lund Hetland, Mikkel Østergaard, Till Uhlig, Marte Schrumpf Heiberg, Espen A. Haavardsholm, Michael T. Nurmohamed, Jon Lampa, Tuulikki Sokka‐Isler, Dan Nordström, Kim Hørslev‐Petersen, Bjorn Gudbjornsson, Gerdur Gröndal, Ronald vanVollenhoven, Hans Carlsten, Mattias Lorentzon, Anna‐Karin Hultgård Ekwall, Anna Rudin, Ulrika Islander

“…Conclusion MTX together with bDMARDs efficiently reduce disease activity but only prevent bone loss in a subset of patients with RA after 48 weeks of treatment. …”
Get full text

Clinical results of using a silver-containing preparation as part of an antimicrobial spacer in the treatment of periprosthetic hip joint infection by Svetlana A. Bozhkova, Magomed Sh. Gadzhimagomedov, Rashid M. Tikhilov, Vasily A. Artyukh, Alexander P. Antipov, Alexander V. Afanasyev, Sergey S. Toropov, Yulia V. Muravyeva

“…The reliability of differences in quantitative parameters between the groups was analyzed using nonparametric Mann – Whitney test, whereas Fisher test was used to analyze relative indicators. …”

Gr-1 blockade remodels the immunosuppressive microenvironment induced by incomplete microwave ablation of hepatocellular carcinoma by Tian Huang, Hensong Cao, Shipeng Dai, Yonghua Zhu, Hanyuan Liu, Shuxian Zhu, Zhengqing Lu, Chuan Liu, Chengyu Lv, Zhouxiao Li, Jinhua Song, Han Zhuo

“…RNA sequencing analysis showed that iMWA can boost chemokine CXCL5, which was further confirmed by quantitative real time polymerase chain reaction (qRT-PCR). …”
Get full text

Optimizing bone transport strategies: a pixel value ratio-based evaluation of regeneration rates in bifocal and trifocal techniques by Xin Yang, Xin Yang, Yimurang Hamiti, Yimurang Hamiti, Kai Liu, Kai Liu, Sulong Wang, Sulong Wang, Xiriaili Kadier, Xiriaili Kadier, Debin Xiong, Debin Xiong, Aihemaitijiang Yusufu, Aihemaitijiang Yusufu

“…This study aimed to compare bone regeneration rates between short bifocal bone transport (SBBT), long bifocal bone transport (LBBT), and trifocal bone transport (TBT) using pixel value ratio (PVR) as an objective quantitative measure.MethodsThis retrospective study included 60 patients undergoing lower limb bone transport, divided into SBBT (n = 22, defects &lt;6 cm), LBBT (n = 20, defects ≥6 cm), and TBT (n = 18, defects ≥6 cm) groups. …”
Get full text

Spectrum of mutations and their phenotypic manifestations in children and adults with long QT syndrome by N. N. Chakova, S. M. Komissarova, E. A. Zasim, T. V. Dolmatovich, E. S. Rebeko, S. S. Niyazova, E. V. Zaklyazminskaya, L. I. Plashchinskaya, M. V. Dudko

“…The average diagnostic efficiency of mutation identification using NGS in patients with clinically manifest LQTS was 66%. …”
Get full text

The protective effects of FeOx/NC nanozymes on chondrocytes by scavenging reactive oxygen species by Jianfeng GUO, Rongwei ZHANG, Zhangrui HUANG, Zhiquan PANG, Shixing LUO

“…Gene and protein expression levels of MMP-13 and IL-6 were measured via reverse transcription-quantitative polymerase chain reaction (RTqPCR), immunofluorescence, and western blotting. …”
Get full text