Outer membrane vesicles of Glaesserlla parasuis activate the endosomal cGAS–STING–IRF3 pathway through nucleic acid payload delivery: a biological perspective on host defense proto... by Kunli Zhang, Zeyi Sun, Xintong Kang, Keda Shi, Pinpin Chu, Dongxia Yang, Zhibiao Bian, Yan Li, Hongchao Gou, Zhiyong Jiang, Nanling Yang, Xia Zhou, Sutian Wang, Zhanyong Wei, Shaolun Zhai, Huahua Kang, Chunling Li

“…Abstract Glaesserlla parasuis (G. parasuis), a Gram-negative pathogen responsible for Glässer's disease, employs outer membrane vesicles (OMVs) as sophisticated nanoscale effectors to modulate host‒pathogen interplay. While bacterial OMVs are recognized as critical mediators of virulence dissemination, their functional orchestration in G. parasuis immunopathogenesis remains unclear. …”
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METTL14-mediated m6A modification enhances USP22-ERα axis to drive breast cancer malignancy by Xuefen Zhuang, Shusha Yin, Ji Cheng, Wenshuang Sun, Zesen Fang, Yujie Xiang, E.-ying Peng, Yu Yao, Yuting Li, Xiaoyue He, Li Lu, Yuanfei Deng, Hongbiao Huang, Gengxi Cai, Yuning Liao

“…Mechanistically, METTL14 potently binds to the USP22 and ERα mRNA, and thereby enhancing their stability through m6A modification. YTHDC1 and YTHDF1 function as readers for m6A-modified USP22 and ERα, respectively. …”
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Identification of SepF in Streptococcus suis involving cell division by Ting Gao, Tingting Li, Jiajia Zhu, Linlin Zheng, Mo Chen, Wei Liu, Keli Yang, Tengfei Zhang, Fangyan Yuan, Zewen Liu, Rui Guo, Chang Li, Qiong Wu, Yongxiang Tian, Rui Zhou, Danna Zhou

“…Results here, we reported a SepF homolog from S. suis that modulated the function of FtsZ. sepF disruption was not lethal and its deletion mutant (∆sepF) displayed normal growth rate. …”
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Coordinated regulation of pH alkalinization by two transcription factors promotes fungal commensalism and pathogenicity by Xinhua Huang, Guangsheng Chen, Lei Wu, Yun Zou, Luyao Zhang, Shanshan Li, Kunlin Li, Zaijie Jiang, Yuping Zhang, Xiaoqing Chen, Winnie Shum, Jianbiao Dai, Huichang Huang, Munika Moses, Xianwei Wu, Yuanyuan Wang, Tong Jiang, Zhiyi He, Qing Guo, Wenwen Xue, Hao Li, Changbin Chen

“…Taken together, our findings elucidate the cooperative function of Dal81 with Stp2 in the nucleus to orchestrate the expression of downstream genes required for the survival and propagation of C. albicans in the host.…”
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Research note: Unveiling the impact of ovotransferrin on chicken primordial germ cells biological processes by Xin Liu, Yixiu Peng, Jun Wu, Hongwu Qian, Xiaoqian Lv, Fan Li, Kai Jin, Yingjie Niu, Jiuzhou Song, Wei Han, Guohong Chen, Bichun Li, Qisheng Zuo

“…These findings reveal a regulatory role of ovotransferrin in maintaining PGCs survival and function, providing new insights into optimizing PGC culture systems through targeted modulation of cell fate.…”
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Research progress on the application of Lacticaseibacillus rhamnosus GG in pediatric respiratory diseases by Yang Liu, Yang Liu, Yang Liu, Yang Liu, Yan Chen, Yan Chen, Yan Chen, Huijuan Liao, Huijuan Liao, Huijuan Liao, Shijie Sun, Shijie Sun, Shijie Sun, Xiaohu Zhang, Xiaohu Zhang, Xiaohu Zhang, Xiaohu Zhang, Liang Xie, Liang Xie, Liang Xie, Liang Xie, Hanmin Liu, Hanmin Liu, Hanmin Liu, Hanmin Liu, Hanmin Liu

“…Its mechanisms include modulating immune responses, enhancing gut barrier function, and maintaining a microbial homeostasis via the gut-lung axis. …”
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Mechanism of SLC1A5 Regulation of Glutamine Metabolism to Promote Ferroptosis Sensitivity in Endometriosis by Hui-Yu Ma, Huan-Yu Wu, Yu-Ting Xiang, Yu-Yin Liu, Jin Xie, Peng-Yu Cai, Bin Zhang, Yu-Hang Zhang, Ming-Xiu Wu

“…Cell viability, ROS levels, glutamine metabolism, mitochondrial function, and ferroptosis markers (glutathione peroxidase 4 (GPX4)) were subsequently analyzed by Cell Counting Kit-8 (CCK-8) assay, reverse transcription quantitative polymerase chain reaction (RT-qPCR), Western blot, and fluorescent probes. …”
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Transient Receptor Potential Vanilloid 4 Knockdown Decreases Extracellular Matrix Synthesis via Autophagy Suppression in the Rat Intervertebral Disc by Tomoya Matsuo, Yoshiki Takeoka, Takashi Yurube, Takeru Tsujimoto, Yutaro Kanda, Kunihiko Miyazaki, Hiroki Ohnishi, Masao Ryu, Naotoshi Kumagai, Kohei Kuroshima, Yoshiaki Hiranaka, Ryosuke Kuroda, Kenichiro Kakutani

“…Our objective was to elucidate the role of TRPV4 in extracellular matrix (ECM) metabolism and autophagy in the rat intervertebral disc through a loss‐of‐function study with the RNA interference (RNAi) technique. …”
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Genetic Variants of Interleukin-8 and Interleukin-16 and Their Association with Cervical Cancer Risk by Rafał Watrowski, Eva Schuster, Stefan Polterauer, Toon Van Gorp, Gerda Hofstetter, Michael B. Fischer, Sven Mahner, Robert Zeillinger, Eva Obermayr

“…Conclusions: These findings highlight the role of age stage in immunity and cancer susceptibility, suggest that IL-8 and IL-16 SNPs may function differently in cervical carcinogenesis compared with other cancers, and emphasize the importance of ethnic background in cancer risk, warranting further research.…”
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Characteristic alterations of gut microbiota and serum metabolites in patients with chronic tinnitus: a multi-omics analysis by Jiang Wang, Jia-Hui Xiang, Xu-Yuan Peng, Min Liu, Le-Jia Sun, Min Zhang, Li-Yuan Zhang, Zhi-Bin Chen, Zheng-Quan Tang, Lei Cheng

“…In serum metabolomic analysis, serum metabolic disturbances in tinnitus patients and these differential metabolites were enriched in pathways of neuroinflammation, neurotransmitter activity, and synaptic function. The predictive models exhibited great diagnostic performance, achieving 0.94 (95% CI: 0.85–0.98) and 0.96 (95% CI: 0.86–0.99) in the test set. …”
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Therapeutic potential of Triptolide in inhibiting breast cancer-induced bone destruction – PTHrP as a therapeutic target by Wu Di, Wu Di, Li Minghan, Li Minghan, Huang Zining, Zhou Chunshi, Hu Xiangyu, Wang Zidong, Wu Gang, Wu Gang, Wu Gang

“…Current treatments, such as bisphosphonates, primarily aim to inhibit osteoclast function but are associated with serious side effects, underscoring the need for alternative therapies. …”
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Prognostic implications of the interaction between intratumoral microbiome and immune response in gastric cancer by Sifan Liu, Lingyu Qi, Lu Dong, Wenjing Sun, Siying Liu, Peng Li, Nan Zhang

“…The key genes were correlated with immune cells and suppressed the function of the host immune system. The intratumoral microbiomes were strongly associated with the hosts’ immune infiltration and significantly interacted with host immune genes to influence GC outcomes. …”
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Targeted delivery of CCL3 reprograms macrophage antigen presentation and enhances the efficacy of immune checkpoint blockade therapy in hepatocellular carcinoma by Min Yang, Wei Wang, Wei Li, Yanan Li, Juan Zhang, Pengfei Rong, Di Wu, Qi Liang, Yao Deng, Xiaoqian Ma, Lu Cao, Muqi Liu, Linzhe Li, Xingshi Gu, Cejun Yang, Huaping Liu

“…High-dimensional single-cell RNA sequencing (scRNA-seq), flow cytometry, and multiplex immunofluorescence were used to investigate CCL3’s effects on macrophage function and T cell activation. The biological impact of CCL3 on macrophages was assessed using co-culture systems, confocal imaging, metabolite detection, and inhibition assays. …”
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LRP6 regulates growth in Litopenaeus vannamei via the Wnt/β-catenin signaling pathway by Sheng Wang, Xiaodi Wang, Hao Yang, Xuanzheng Di, Haoyang Li, Bin Yin, Ziwei Liu, Chaozheng Li, Jianguo He

“…Multiple sequence alignments and phylogenetic analyses indicated that LvLRP6 belongs to the invertebrate LRP6 family and has conserved phosphorylation sites with those of LRP6 in other species. Functional investigations via RNAi technology revealed that silencing LvLRP6 via dsRNA injection resulted in a significant reduction in shrimp growth, as indicated by lower weight gain than that in the control group. …”
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Hypoxia preconditioned MSC exosomes attenuate high-altitude cerebral edema via the miR-125a-5p/RTEF-1 axis to protect vascular endothelial cells by Jia-Chen Zuo, Jun Liang, Nan Hu, Bin Yao, Qi-Jian Zhang, Xiao-Li Zeng, Ling-Jie Zhang, Xu Zhang, Zhe-Han Chang, Chong Chen, Xin-Jian Yan, Wen-Wei Shao, Ping Zhu, Xiao-Hong Li

“…The therapeutic effects manifested through three principal aspects: 1) downregulation of RTEF-1/VEGF hyperexpression, 2) modulation of VE-cadherin, SMA, and PDGFRα + β expression to preserve BBB integrity, and 3) concurrent protection of neurovascular functions against HACE-induced damage. …”
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E3 ubiquitin ligase RNF128 attenuates colitis and colorectal tumorigenesis by triggering the degradation of IL-6 receptors by Tian-Sheng He, Kuntai Cai, Weiling Lai, Jingge Yu, Furong Qing, Ao Shen, Lina Sui, Wenji He, Weihua Wang, Qiuxiang Xiao, Xiong Lei, Tianfu Guo, Zhiping Liu

“…Objectives: To elucidate the function and mechanism of RNF128 in colitis and CRC. …”
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An ALPK3 truncation variant causing autosomal dominant hypertrophic cardiomyopathy is partially rescued by mavacamten by Lisa Leinhos, Paul Robinson, Giulia Poloni, Sophie Broadway-Stringer, Julia Beglov, Adam B. Lokman, Gillian Douglas, Sajjad Nuthay, Oveena Fonseka, Manuel Schmid, Evie Singer, Charlotte Hooper, Kate Thomson, Richard D. Bagnall, Jodie Ingles, Christopher Semsarian, Elizabeth Ormondroyd, Christopher N. Toepfer, Benjamin Davies, Charles Redwood, Hugh Watkins, Katja Gehmlich

“…Abstract The ALPK3 gene encodes alpha-protein kinase 3, a cardiac pseudo-kinase of unknown function. Heterozygous truncating variants (ALPK3tv) can cause dominant adult-onset hypertrophic cardiomyopathy (HCM). …”
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ARID1A is a coactivator of STAT5 that contributes to CD8+ T cell dysfunction and anti-PD-1 resistance in gastric cancer by Fangqi Ma, Mingming Ren, Zhongqiu Li, Yujing Tang, Xiaoyu Sun, Yi Wang, Nida Cao, Xiaohong Zhu, Yan Xu, Rui Wang, Yumiao Shen, Ruohan Zhao, Zhaoyan Li, Milad Ashrafizadeh, Gautam Sethi, Furong Wang, Aiguang Zhao

“…However, its role in modulating of tumor immune microenvironment (TIME) of gastric cancer (GC) remains unclear. …”
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Glycolytic flux controls retinal progenitor cell differentiation via regulating Wnt signaling by Joseph Hanna, Yacine Touahri, Alissa Pak, Lauren Belfiore, Edwin van Oosten, Luke Ajay David, Sisu Han, Yaroslav Ilnytskyy, Igor Kovalchuk, Deborah Kurrasch, Satoshi Okawa, Antonio del Sol, Robert A Screaton, Isabelle Aubert, Carol Schuurmans

“…In RPC-specific Phosphatase and tensin homolog conditional knockout (Pten-cKO) and RPC-specific conditional gain-of-function of dominant active PFKFB3 (cytoPFKFB3) mice, glycolytic gene expression and activity are elevated, correlating with precocious rod photoreceptor differentiation and outer segment (OS) maturation. …”
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Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system by Shijie Hu, Shijie Hu, Mailin Gan, Mailin Gan, Ziang Wei, Ziang Wei, Pan Shang, Pan Shang, Lei Song, Lei Song, Jinkang Feng, Jinkang Feng, Lei Chen, Lei Chen, Lili Niu, Lili Niu, Yan Wang, Yan Wang, Yan Wang, Shunhua Zhang, Shunhua Zhang, Linyuan Shen, Linyuan Shen, Linyuan Shen, Li Zhu, Li Zhu, Li Zhu, Ye Zhao, Ye Zhao, Ye Zhao

“…Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. …”
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