Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis in Bupleurum chinense DC by Han Wang, Shanqun Hu, Tong Li, Xuejie Qu, Jiaqi Zhang, Baoshun Wang, Yixuan Sun, Rui Cao, Yutong Yan, Ze Song, Xia’nan Zhang, Rong Luo, Yuru Tong, Changli Liu

“…Furthermore, precision engineering of the critical functional domain in BcbHLH could enhance promoter-binding activity to target genes and improve SS biosynthesis efficiency. …”
Get full text

Effects of in vitro combination therapy with polymyxin B on delaying resistance in Klebsiella pneumoniae and insights into the mechanisms of polymyxin-induced resistance by Huanhuan Zhang, Wei Feng, Fengjun Sun, Te Xu, Yan Qian

“…Then, gene knockout experiments were performed to validate the functional roles of relevant genes. Results: These findings indicated that PMB alone could induce resistance within 1–2 d, whereas the combination with amikacin (AMK) or tigecycline delayed the onset of resistance by 6 d. …”
Get full text

Benefits of Probiotics—Biodetoxification by Barbara Sionek, Aleksandra Szydłowska, Danuta Jaworska, Danuta Kołożyn-Krajewska

“…In addition, probiotics in food are associated with well-known human health benefits; therefore, as a functional food, they have gained common consumer acceptance. …”
Get full text

Theoretical study on the alkyl chain length impact of azobenzene-based photoresponsive ionic liquids by Liyao Ying, Zhaoyang Ju, Chunjin Lin, Pin Wang, Haixiang Cheng, Liyong Ding, Tianshu Chu, Chunxiao Gong, Bing Zhou, Mengting Yu, Chengsheng Ge

“…Abstract The light-modulated isomerization and aggregation behavior of ionic liquids (ILs) in aqueous solutions holds fundamental and technological significance. …”
Get full text

Bio-inspired natural fibers-derived e-skin equipped with intelligent drug-release system for advanced robustly-integrated melanoma therapy by Xinhua Liu, Yifan Fei, Boqiang Cui, Xing Chen, Jiamin Zhang, Ouyang Yue, Zhongxue Bai, Ling Wen, Huie Jiang

“…Notably, pH-responsive Cu2⁺ liberation from CuS-NPs facilitates intelligent functional switching: bactericidal activity at tumor microenvironment pH (5.0–6.0) and pro-regenerative effects under physiological pH (7.4) for wound healing. …”
Get full text

Targeting inflammation in cancer therapy: from mechanistic insights to emerging therapeutic approaches by Saad Bakrim, Meriem El Fessikh, Hamza Elhrech, Nasreddine El Omari, Mohammed Amanullah, Long Chiau Ming, Said Moshawih, Abdelhakim Bouyahya

“…However, inflammatory cells in the TME exhibit remarkable plasticity, with shifting phenotypic and functional roles that facilitate cancer cell survival, proliferation, and migration, especially under chronic inflammatory conditions. …”
Get full text

Metabolic profiling and anti-neuroinflammatory effects of Liriope platyphylla extract in LPS-stimulated BV2 microglia by Kwan-Woo Kim, Woo-Cheol Shin, Jin-Kyu Jang, Myoung-Jin Kim, Dahye Yoon, Young-Seob Lee, Dae Young Lee

“…Collectively, these findings highlight the promise of LPE as a source for developing novel functional ingredients or herbal therapeutics targeting neuroinflammatory conditions.…”
Get full text

Decoding oral cancer: insights from miRNA expression profiles and their regulatory targets by Xin Wang, Xin Wang, Shuang Zhang, Shuang Zhang, Shuyi Wang, Tao Cao, Tao Cao, Hong Fan, Hong Fan

“…A total of 23 differentially expressed miRNAs (DEmiRs) and 1,233 differentially expressed genes (DEGs) were identified. Functional annotation and pathway enrichment analyses highlighted significant involvement of DEmiRs and their target genes in cell cycle-related processes, including enrichment in the nucleus, transcription factor activity, regulation of nucleosides, nucleotide and nucleic acids, cell growth and/or maintenance, mitotic cell cycle, mitotic M-M/G1 phases an DNA replication. …”
Get full text

CCDC138 overexpression predicts poor prognosis and highlights ciliopathy-linked mechanisms in uterine corpus endometrial carcinoma by Aiping Wang, Fang Yang, Chunhua Zhang, Shi Li, Han Fu

“…Bioinformatics approaches, including weighted gene co-expression network analysis (WGCNA), singlesample gene set enrichment analysis (ssGSEA), machine learning, and survival analysis, were employed to assess CCDC138 expression and its functional relevance in UCEC. In vitro experiments involved CCDC138 knockdown, followed by CCK8 and EdU assays and qPCR for mTOR, S6K1, and p21 expression.ResultsCCDC138 was significantly overexpressed at mRNA and protein levels in UCEC and correlated with poor overall survival. ssGSEA revealed associations with oncogenic pathways, including mTOR, p53/Rb, and MYC/MYCN. …”
Get full text

Conserved LIR-specific interaction of Sigma-1 receptor and GABARAP by Marius Wilhelm Baeken, Maximilian Christ, Daniel Schmitt, Wencke Trein, Heike Nagel, Albrecht Martin Clement, Hagen Körschgen, Christian Behl

“…Summary: Among its various functions, the sigma-1 receptor (σ1R) has been reported to modulate macroautophagy. …”
Get full text

Knockdown of RGMa reduces vascular calcification by inhibiting the AKT signaling pathway by Hongmei Xiao, Guanru Shen, Zijie Wang, Xiaofan Yuan, Yue Ma, Ruiqi Cheng, Xinyue Qin

“…This study indicates that RGMa may promote VC by modulating AKT activation, highlighting RGMa as a promising new molecular target for the treatment of VC.…”
Get full text

Comprehensive landscape of small non‐coding RNAs in large and small extracellular vesicles from human serum and plasma by Gui‐Yan Xie, Yi‐Han Deng, Chun‐Jie Liu, An‐Yuan Guo, Qian Lei

“…Abstract Small non‐coding RNAs (ncRNAs) are functional molecules contained within extracellular vesicles (EVs) that modulate various physiological and pathological processes. …”
Get full text

Association studies of ERCC1 polymorphisms with lung cancer susceptibility: a systematic review and meta-analysis. by Jinhong Zhu, Rui-Xi Hua, Jing Jiang, Li-Qin Zhao, Xiuwei Sun, Jinwei Luan, Yaoguo Lang, Yanqi Sun, Kun Shang, Shiyun Peng, Jianqun Ma

“…<h4>Background</h4>Excision repair cross-complimentary group 1 (ERCC1) is an essential component of the nucleotide excision repair system that is responsible for repairing damaged DNA. Functional genetic variations in the ERCC1 gene may alter DNA repair capacity and modulate cancer risk. …”
Get full text

Depletion-restitution therapy of autoimmune rheumatic diseases. Part 2. Perspectives on bispecific antibodies by A. L. Maslyanskiy, D. A. Dibrov, A. M. Lila, A. V. Torgashina, E. G. Zotkin, M. Yu. Samsonov, G. A. Togizbayev

“…Recent years have seen attempts to repurpose bsAbs for the treatment of refractory, prognostically unfavorable forms of systemic autoimmune rheumatic diseases (SARDs), supported by theoretical rationale, experimental evidence, and parallels with successful CAR-T cell therapy.Beyond BiTEs, the bsAb platform also enables development of biologics with extended pharmacokinetics, multi-cytokine targeting potential for synergistic suppression of inflammation, and checkpoint-directed modulation of targeted cell functional activity.Advantages such as standardized manufacturing, off-the-shelf availability, predictable pharmacokinetics (with a known and limited half-life), flexible dosing regimens enabling slow escalation of the dose, the possibility of individualizing treatment duration and dosing frequency, the feasibility of repeated treatment cycles, the option to discontinue therapy in case of adverse events, and the significantly lower cost of short low-dose treatment cycles compared to CAR-T cell therapy – all these make bsAb-based strategies a highly attractive priority for next-generation depletion-restitution therapies for SARDs.…”
Get full text

Unveiling Prophage Diversity and Host Interactions in Liberibacter: Genomic Insights for Phage Therapy Against Citrus Huanglongbing by Hui Yin, Jiaxing Wan, Siyu Zhang, Zhuozhuo Wu, Wanshan Zhang, Yuxia Gao

“…Annotation showed that the genome sizes of NF1–NF13 ranged from 10,160 bp to 72,736 bp, with an average size of 27,616 bp, containing between 7 and 66 genes (average: 27 genes). Further functional enrichment of the sequences within 5 kb upstream and downstream of the integration site identified six main aspects, involving four core biological processes: genome structure modification, gene expression regulation, stress response activation, and metabolic pathway modulation. …”
Get full text

Research and progress of microRNA-136 in metastatic tumors by Chenwen Wang, Zixiong Chen, Wei Ni, Jiang Wang, Wei Zhou

“…This review summarizes current knowledge regarding miR-136’s molecular mechanisms, functional roles, and impact on chemotherapy in different human cancers.MethodsA literature search was conducted in PubMed and Web of Science using “miR-136” and “metastatic tumors” as English keywords, and in CNKI and Wanfang databases using the same terms in Chinese. …”
Get full text

Pathophysiological mechanisms in severe preeclampsia: role of upregulated proteins in blood pressure, extracellular matrix and immunity by Caroline Cristina Pinto-Souza, Julyane Natsumi Saito Kaihara, Bruno César Rossini, Ricardo de Carvalho Cavalli, Lucilene Delazari dos Santos, Valéria Cristina Sandrim

“…Multivariate statistical analyses, including PLS-DA and sPLS-DA, as well as VIP score evaluation and Volcano plot visualization, were conducted with MetaboAnalyst to assess group separation and identify key discriminative features. Functional enrichment and pathway analyses were carried out using Metascape. …”
Get full text

Low regulatory T-cells frequency is associated with graft rejection after small bowel transplantation: Clinical and experimental evidence. by Rodrigo Papa-Gobbi, Pablo Stringa, Maria Virginia Gentilini, Ivana Ivanoff, Mariana Machuca, Nidia Monserrat Arreola, Javier Serradilla, Karla Estefanía-Fernández, Paloma Talayero, María Velayos, Elena Sánchez-Zapardiel, Gabriel Gondolesi, Ane Andrés-Moreno, Martin Rumbo, Francisco Hernández-Oliveros

“…<h4>Results</h4>Blood samples from patients undergoing ACR exhibited a significant reduction in the Treg number compared to those with normo-functional grafts. Similarly, a diminished number of FoxP3+ cells was observed in mucosa samples with ACR. …”
Get full text

Causes and consequences of individual variation: Linking state‐dependent life histories to population performance by Marc A. Wiseman, Kevin L. Monteith, Ryan A. Long

“…Abstract Although classic ecological models often have assumed functional equivalence among individuals in a population, ecologists now recognize that individual variation can modulate ecological processes across levels of organization. …”
Get full text

Identification of therapeutic targets in lung adenocarcinoma using Mendelian randomization and multi-omics by Yue Li, Keru Ma, Hao Wang, Zongying Liu, Zhuying Li

“…The prognostic relevance of the identified candidate targets was assessed using transcriptomic data. Functional interactions were explored via protein–protein interaction network analysis, while single-cell analyses were employed to determine cell-specific expression patterns and differentiation trajectories. …”
Get full text