Genome Editing of the CYP1A1 Locus in iPSCs as a Platform to Map AHR Expression throughout Human Development by Brenden W. Smith, Elizabeth A. Stanford, David H. Sherr, George J. Murphy

“…This cell line faithfully reports on CYP1A1 expression, with luciferase levels as its functional readout, when treated with an endogenous AHR ligand (FICZ) at escalating doses. iPSC-derived fibroblast-like cells respond to acute exposure to environmental and endogenous AHR ligands, and iPSC-derived hepatocytes increase CYP1A1 in a similar manner to primary hepatocytes. …”
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Beyond biomedical frontiers: Multidimensional applications of silk and its derived proteins in energy, environment, and agri-food systems by Huanyan Ma, Zhaoyang Wang, Meiliang Pan, Fengyang Hu, Guangdong Sun, Xiuling He

“…Recent breakthroughs in genetic engineering (CRISPR-edited silkworms) and nanocomposite fabrication (carbon nanotube hybrids) are critically analyzed for their roles in overcoming historical limitations in batch consistency and functional integration. The application survey spans (1) flexible electronics utilizing silk’s dielectric tunability for epidermal sensors, (2) biophotonic devices exploiting humidity-responsive optical modulation, (3) environmental technologies for air/water purification, and (4) agricultural innovations through silkworm by-product valorization. …”
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Mitochondrial fission regulator 2 promotes cell proliferation, migration and invasion in hepatocellular carcinoma through regulating PI3K/AKT signaling pathway by Qiang Sun, Kaikun Liu, Fangjie Zheng, Xinwei Chen

“…Validation of MTFR2 expression levels in clinical samples and HCC cell lines was performed through qRT-PCR and western blot. Functional effects of MTFR2 overexpression and knockdown on HCC cell proliferation, migration, and invasion were assessed via CCK-8, colony formation, wound healing, and transwell assays. …”
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Extracellular vesicle-based drug overview: research landscape, quality control and nonclinical evaluation strategies by Gangling Xu, Jing Jin, Zhihao Fu, Guangming Wang, Xinhua Lei, Jun Xu, Junzhi Wang

“…Abstract Extracellular vesicles share lipid‒protein membranes with their parent cells, allowing for the targeted transfer of bioactive cargo to recipient cells for functional modulation. The biological features allow extracellular vesicles to serve both as intrinsic therapeutics and as engineered delivery vehicles for targeted molecule transport. …”
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Decoding mechanosensitive genes in cardiac fibroblasts via 3D hydrogel models of fibrosis by Heng Chen, Yuxiao Chen, Jinan Yang, Peng Yang, Hongqiang Cheng, Xiaogang Guo

“…Notably, the marked upregulation of this gene cluster in human dilated and hypertrophic cardiomyopathy samples underscores its clinical relevance. Functional assays confirmed the crucial roles of THBS2 and DCN in fibroblast activation. …”
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Microbial Crosstalk with Therapy: Pharmacomicrobiomics in AML—One Step Closer to Personalized Medicine by Aneta Nowicka, Hanna Tomczak, Edyta Szałek, Agnieszka Karbownik, Lidia Gil

“…Multi-omics technologies have provided qualitative and mechanistic insights into specific compositional and functional microbial signatures associated with the outcomes of AML therapy, but require a large-scale investigation to draw reliable conclusions. …”
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Momentum-locked spin between topological and defect states in 1D patterns on bilayer graphene by R. Guerrero-Avilés, A. Ayuela, Leonor Chico, W. Jaskólski, M. Pelc

“…In the calculations we consider electron-electron interaction effects using density functional theory. Our findings reveal the existence of topological states within a gap induced by the patterning without an applied gate voltage. …”
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Machine learning-based meta-analysis reveals gut microbiome alterations associated with Parkinson’s disease by Stefano Romano, Jakob Wirbel, Rebecca Ansorge, Christian Schudoma, Quinten Raymond Ducarmon, Arjan Narbad, Georg Zeller

“…These results align with epidemiological evidence that exposure to these molecules increases PD risk and raise the question of whether gut microbes modulate their toxicity. Here, we offer the most comprehensive overview to date about the PD gut microbiome and provide future reference for its diagnostic and functional potential.…”
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An IoT-enabled monitoring and control system for electrochromic smart windows by Hsien-Hern Koay, Gregory Soon How Thien, Benedict Wen-Cheun Au, Abdelrahman Hamed Ebrahem Abdelhamed, Ruthramurthy Balachandran, Zi-Neng Ng, Kah-Yoong Chan

“…This study also provided a functional design analysis for a Wi-Fi-enabled control system, presented insights into manual and automatic control modes under diverse environmental conditions, and improved user interaction through real-time data visualization using the IoT Blynk platform. …”
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Unveiling shared therapeutic targets and pathological pathways between coronary artery disease and major depressive disorder through bioinformatics analysis by Mengyun Hu, Rong Tan, Caihong Lu, Ting Zhou, Qin Wang, Tao Liu

“…However, the shared mechanisms and therapeutic targets for CAD and MDD remain incompletely comprehended. Functional enrichment analyses were conducted to investigate the pathways associated with the differentially expressed genes (DEGs) in the CAD and MDD datasets. …”
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ANGPTL4 suppresses progression and improves cisplatin sensitivity in cervical cancer by Waraporn Chan-on, Methawadee Turinthorn, Arkom Chaiwongkot, Teerasit Techawiwattanaboon, Prasong Khaenam, Chaniya Leepiyasakulchai

“…Our analysis revealed a decreased expression of ANGPTL4 in CC samples from the GSE dataset and in the CC cell lines examined. Functional assays demonstrated that ANGPTL4 overexpression suppressed CC cell proliferation, migration, and invasion. …”
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Identifying Hub Genes and miRNAs Associated with Alzheimer’s Disease: A Bioinformatics Pathway to Novel Therapeutic Strategies by Elisa Gascón, Ana Cristina Calvo, Nora Molina, Pilar Zaragoza, Rosario Osta

“…Therefore, in this study, by means of bioinformatics analysis, AD-affected brain tissue was analyzed using the GSE138260 dataset, identifying 612 differentially expressed genes (DEGs). Functional analysis revealed 388 upregulated DEGs associated with sensory perception and 224 downregulated DEGs linked to the regulation and modulation of synaptic processes. …”
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Large-scale CRISPRi screens link metabolic stress to glioblastoma chemoresistance by Xing Li, Wansong Zhang, Yitong Fang, Tianhu Sun, Jian Chen, Ruilin Tian

“…Molecular mechanisms were investigated through transcriptome analysis, metabolic profiling, and functional assays. Results We identified phosphoglycerate kinase 1 (PGK1) as a key determinant of TMZ sensitivity. …”
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Evaluation of dysphagia severity in patients with nasopharyngeal carcinoma undergoing radiotherapy by Mahmood Bahramizadeh, Rezvaneh NAMAZI Yousefi, Francesco Ciodaro

“…The aim of this study was to identify specific foods and feeding methods recommended for these patients and to evaluate the anatomical and functional structures most affected by radiotherapy doses. …”
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Melatonin Elicitation Differentially Enhances Flavanone and Its Endogenous Content in Lemon Tissues Through Preharvest and Postharvest Applications by Vicente Agulló, María Emma García-Pastor, Daniel Valero

“…The results of this study demonstrate the efficacy of melatonin elicitation, particularly in postharvest treatments, as a practical strategy to enhance the functional quality of lemons. This approach has the potential to facilitate the development of health-promoting foods and the valorisation of citrus byproducts. …”
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Orphan Nuclear Receptors TR2 and TR4 in Erythropoiesis: From Mechanisms to Therapies by Yunlong Liu, Helian Yang, Mengtian Ren, Qing Yu, Qingyang Xu, Xiuping Fu

“…These nuclear receptors not only engage in functional interactions with key erythroid transcription factors GATA1 and KLF1 to coregulate erythroid differentiation and maturation but also recruit epigenetic modifier complexes such as DNMT1 and LSD1 to modulate chromatin states dynamically. …”
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Mesenchymal stem cell‐derived exosomes: Shaping the next era of stroke treatment by Arshi Waseem, Saudamini, Rizwanul Haque, Miroslaw Janowski, Syed S. Raza

“…Their diverse effects include neuroprotection, angiogenesis stimulation, inflammatory response modulation, and cell death pathway attenuation, synergistically promoting neuronal survival, tissue regeneration, and functional recovery. …”
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Optical segmentation-based compressed readout of neuronal voltage dynamics by Seonghoon Kim, Jongmin Yoon, Gwanho Ko, Iksung Kang, He Tian, Linlin Z. Fan, Yixin Li, Guihua Xiao, Qi Zhang, Adam E. Cohen, Jiamin Wu, Qionghai Dai, Myunghwan Choi

“…Abstract Functional imaging of biological dynamics generally begins with acquiring time-series images, followed by quantifying spatially averaged intensity traces for the regions of interest (ROIs). …”
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Condition-controlled divergent trifluoroalkylation: a diversity-oriented synthesis strategy for efficient construction of CF3-decorated carbazole libraries by Peng Chen, Jin Yin, You-Hui Tang, Meng-Yuan Li, Yi-Jun Jiang

“…This work achieves the synthesis of a library of structurally diverse CF3-decorated carbazole derivatives from the same set of readily available substrates through systematic modulation of reaction conditions. The strategy not only provides a versatile platform for synthesizing fluorinated carbazole architectures but also inspires future exploration of condition-driven diversity-oriented synthesis (DOS) of carbazole derivatives with tailored functional groups for applications in medicinal chemistry and materials science.…”
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Emerging roles of tsRNAs in programmed cell death and disease therapeutics: challenges, opportunities, and future directions by Zhe Li, Bo Zhang, Yanru Pan, Qiuyan Weng, Kefeng Hu

“…Despite challenges in understanding tsRNA biogenesis and functional diversity, their roles in regulating PCD highlight their strong potential in advancing disease diagnostics, treatment strategies, and personalized medicine.…”
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