GrpEL1 overexpression mitigates hippocampal neuron damage via mitochondrial unfolded protein response after experimental status epilepticus by Minjia Xie, Xin Wu, Xi Liu, Longyuan Li, Feng Gu, Xinyu Tao, Bingyi Song, Lei Bai, Di Li, Haitao Shen, Zongqi Wang, Wei Gao

“…Neuronal survival was evaluated through Nissl staining. Mitochondrial function was evaluated aggresome formation, mitochondrial membrane potential (MMP) assays, mitochondrial oxygen consumption rate (OCR) measurements, and behavioral assessments using the Morris water maze. …”
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Generation of an inducible dCas9-SAM human PSC line for endogenous gene activation by Paolo Petazzi, Francisco Gutierrez-Agüera, Heleia Roca-Ho, Julio Castaño, Clara Bueno, Clara Bueno, Clara Bueno, Niuska Alvarez, Lesley M. Forrester, Ana Sevilla, Ana Sevilla, Antonella Fidanza, Antonella Fidanza, Pablo Menendez, Pablo Menendez, Pablo Menendez, Pablo Menendez, Pablo Menendez

“…The engineered H9-iCas9.SAM line provides a robust platform for investigating gene function and advancing next-generation cell-based therapies.…”
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BUILING THE INFLAMMATORY PROCESS IN BRONCHES AT THE PHASE OF PATIENTS REHABILITATION WITH PROFESSIONAL CHRONIC OBSTRUCTIVE LUNG DISEASE by Раиса Васильевна Гордеева, Ольга Васильевна Кузьменко, Сергей Николаевич Филимонов, Лиана Николаевна Киреева, Елена Викторовна Воеводина

“…Before and after the treatment, all the patients were examined for local immunity of the nasal mucosa and sputum: the concentrations of secretory immunoglobulin A (SIgA) and the activity of lysozyme (AL), cytograms of impression smears of nasal secretions and sputum with the definition of cellular composition (cylindrical epithelial (CE) and flat epithelial (FE) cells, alveolar macrophages (AM), neutrophils (N)), as well as functional activity of neutrophils (FAN), functional activity of alveolar macrophages (FAAM). …”
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Identification of TMEM100’s role in immune infiltration regulation and construction of an endogenous RNA network in prostate cancer through pan-cancer analysis by Yinfeng Jiang, Tingan Pan, Zhenglin Zhang

“…Furthermore, a TMEM100-related ceRNA network was constructed in PRAD, corroborating TMEM100’s role in PCa through functional experiments. Conclusion TMEM100 downregulation, through modulation of immune infiltration and epigenetic mechanisms, correlates with poor prognosis and tumor cell proliferation in PRAD.…”
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Macrophages as Multifaceted Orchestrators of Tissue Repair: Bridging Inflammation, Regeneration, and Therapeutic Innovation by Wang L, Yang K, Xie X, Wang S, Gan H, Wang X, Wei H

“…Recognizing this mechanoregulation broadens therapeutic avenues for precisely modulating macrophage behavior in regenerative medicine. …”
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Comparative microbiome analysis of paired mucosal and fecal samples in Korean colorectal cancer patients by Younggwang Kim, Min Ki Kim, Sanghun Lee, Sanghun Lee, Sanghun Lee

“…Combined microbial markers yielded an AUC of 0.841 for distinguishing pre- from post-surgical status. Functional predictions indicated upregulation of amino acid metabolism and lipopolysaccharide (LPS) biosynthesis pathways in pre-surgical samples. …”
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Unveiling the role of coagulation-related genes in acute myeloid leukemia prognosis and immune microenvironment through machine learning by Liyun Ji, Yanxia Yang, Siyue Ma

“…We further identified differentially expressed genes (DEGs) between groups and conducted functional enrichment analyses. Additionally, a prognostic model was constructed using machine learning techniques, and its prognostic ability was validated. …”
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GPx3 marks adipocyte lineage commitment in bone marrow stromal cells by Zhongxiang Wang, Yangyang Hu, Mao Li, Xiaojun Chen, Chengyu Zhou, Zhiyang Xu, Kai Chen, Lichuang Wu

“…Furthermore, re-analysis of single-cell RNA sequencing (scRNA-seq) data was employed to determine the cell type–specific expression of candidate genes within the bone marrow. Functional assays using RNA interference were carried out to investigate the role of glutathione peroxidase 3 (GPx3) in adipogenesis. …”
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Exosome Secretion Regulation in Mice with Acute Lung Injury by Jie HH, Lu WH

“…Exosomes secreted by lipopolysaccharide—treated rat type II alveolar epithelial cells stimulated the polarization of M1 macrophages, which resulted in morphological and functional changes.Conclusion: Exosome Secretion Regulation in Mice with Acute Lung Injury.Keywords: sepsis, C—C motif chemokine ligand 2, CCL2, exosome, lung macrophages, acute lung injury…”
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Brief exposure to obesogenic diet disrupts brain dopamine networks. by Robert L Barry, Nellie E Byun, Jason M Williams, Michael A Siuta, Mohammed N Tantawy, Nicole K Speed, Christine Saunders, Aurelio Galli, Kevin D Niswender, Malcolm J Avison

“…<h4>Methods</h4>We examined the effects of a two-week HF diet on striatal DAT activity in rats, using AMPH as a probe in a functional magnetic resonance imaging (fMRI) assay, and mapped the disruption in AMPH-evoked functional connectivity between key dopaminergic targets and their projection areas using correlation and permutation analyses. …”
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MYST2 histone acetyltransferase promotes lung adenocarcinoma progression by regulating the p38 MAPK signaling pathway by Zhiang Huang, Wanru Zhang, Ping Wang, Mengyao Wu, Yipu Guo, Jingying Chen

“…Methods: An analysis using the TIMER 2.0 and TCGA databases was performed to compare the expression levels of MYST2 between lung adenocarcinoma tissues and normal tissues. Functional assays, including cell proliferation, migration, and invasion, were conducted to evaluate the effects of MYST2 overexpression and knockout in lung cancer cells. …”
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Effects of proteasome inhibitor MG-132 on the parasite Schistosoma mansoni. by Enyara R Morais, Katia C Oliveira, Renato G de Paula, Alice M M Ornelas, Érika B C Moreira, Fernanda Rafacho Badoco, Lizandra G Magalhães, Sergio Verjovski-Almeida, Vanderlei Rodrigues

“…Here, we used expression microarrays to measure the genome-wide changes in gene expression of S. mansoni adult worms exposed in vitro to MG-132, followed by in silico functional analyses of the affected genes using Ingenuity Pathway Analysis (IPA). …”
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The mechanism of Bletilla striata inhibiting glioma proliferation through the PI3K/AKT/mTOR signaling pathway based on network Pharmacology analysis by Fenglian Ren, Yihua Wang, Changyang He, Xi Zheng, Chunyang Zhou, Xianmei Zhong

“…A “drug-ingredient-target” network and protein–protein interaction (PPI) network were constructed, with core targets identified via topological analysis. Functional enrichment (GO/KEGG, DAVID), molecular docking, and experimental validations (MTT, scratch assay, RT-PCR, Western blot) were performed. …”
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Effect of electroacupuncture on restoration of traumatic vertebral compression fracture: two case studies and literature review by Ji-Sung Yeum, Ye-Rim Yun, No-Hyun Kim, Jong-Ho Choi, Do-Young Kim, Do-Young Kim

“…Vertebral compression fractures (VCFs) cause severe pain and functional impairments. Conventional treatments, including medication and vertebral augmentation, have limited efficacy and safety. …”
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Advances in the regulation of macrophage polarization by the tumor microenvironment by Minzhi Peng, Yuan Zhu, Yi Hu, Jianping Wen, Weiguo Huang

“…This review aims to systematically elucidate the characteristics and functional differences of macrophage polarization into M1 and M2 phenotypes within the TME. …”
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SNRPB2 facilitates esophageal squamous cell carcinoma oncogenesis and progression via E2F4 stabilization by Feng Xu, Chen-Cheng Zhu, Chen Lu, Guang-Yao Ning, Ren-Quan Zhang

“…Immune-related and functional analyses were performed using TIMER, TISIDB, TISCH, Gene Ontology (GO), and Gene Set Enrichment Analysis (GSEA). …”
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Environmental drivers of the resistome across the Baltic Sea by Joeselle M. Serrana, Francisco J. A. Nascimento, Benoît Dessirier, Elias Broman, Malte Posselt

“…In this study, we characterized the environmental resistome of the Baltic Sea and evaluated how environmental gradients and spatial variability, alongside its microbial communities and associated functional genes, influence resistome diversity and composition across geographic regions. …”
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The approaches to prevention and therapy of cardiovascular disorders in children with chronic bronchopulmonary pathology by G. O. Lezhenko, О. Ye. Pashkova

“…It has been established that there is a structural and functional left ventricular myocardium rearrangement on the background of left ventricular diastolic dysfunction development, sympathetic branch of the autonomic nervous system functional impairment, myocardium electrical instability and endothelium-depended vasodilation disturbance in children with chronic bronchopulmonary diseases. …”
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The double-edged role and therapeutic potential of TREM2 in atherosclerosis by Botao Zhu, Yuxuan Liu, Daoquan Peng

“…Understanding these dual roles could help in the development of new therapeutic strategies to modulate TREM2 activity and utilize its atheroprotective function while mitigating its deleterious effects. …”
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Learning epigenetic regulation from mycobacteria by Sanjeev Khosla, Garima Sharma, Imtiyaz Yaseen

“…We describe the identification of a methyltransferase from M. tuberculosis that functions to modulate the host epigenome by methylating a novel, non-canonical arginine, H3R42 in histone H3. …”
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