Generation of the MCF-7 cell sublines with CRISPR/Cas9 mediated disruption of estrogen receptor alfa (ESR1) expression by L. O. Savinska, S. A. Kvitchenko, S. S. Palchevskyi, I. V. Kroupskaya, A. V. Mazov, O. M. Garifulin, V. V. Filonenko

“…By applying two distinct types of gRNA for gene editing, we established six MCF-7 cell sublines with either nearly complete or partial down-regulation of the ESR1 isoforms. Unexpectedly, the data obtained revealed no discernible impact of ESR1 down-regulation on EMT manifestation as Western blot and Real-Time qPCR analysis of selected clones revealed no changes in EMT markers expression. …”
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Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia by G. E. Saraceno, R. Castilla, G. E. Barreto, J. Gonzalez, R. A. Kölliker-Frers, F. Capani

“…Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works, we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long-term ubi-protein accumulation. …”
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GUEST EDITORS’ NOTE by Piero Amodio, Edward W. Legg, Ljerka Ostojić

“…This paper also illustrates how similar patterns may be observed in the timings of human behaviour and calls on researchers to better investigate the timing and organisation of behaviour – one part of which could potentially be achieved through secondary data analysis of timings in open behavioural data sets. …”
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Remote ischaemic conditioning for efficacy in patients with aneurysmal subarachnoid haemorrhage (REPAIR): protocol for a multicentre, randomised, double-blind, sham-controlled, par... by Yunjian Yin, Xunming Ji, Aihua Liu, Xin Feng, Hao Niu, Lan Liu, Wenbo Zhao, Tonghu Jin, Liuyu Xu, David C Hess

“…However, the neuroprotective effect of RIC on subarachnoid haemorrhage is still lacking convincing evidence and deserves further study.Aim To explore the efficacy and safety of RIC for aneurysmal subarachnoid haemorrhage.Methods and analysis An investigator-initiated, multicentre, randomised, double-blind, sham-controlled, parallel-group trial to evaluate the effect of RIC intervention compared to sham RIC intervention for the neurological function improvement of patients with aneurysmal subarachnoid haemorrhage.Ethics and dissemination The protocol was approved by the IRB of Beijing Tiantan Hospital, Capital Medical University (KY2024-080-03). …”
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Identification of CENPM as a key gene driving adrenocortical carcinoma metastasis via physical interaction with immune checkpoint ligand FGL1 by Cunru Zou, Yu Zhang, Chengyue Liu, Yaxin Li, Congjie Lin, Hao Chen, Jiangping Hou, Guojun Gao, Zheng Liu, Qiupeng Yan, Wenxia Su

“…Methods Weighted gene co‐expression network analysis (WGCNA) and functional enrichment analysis were conducted to identify ACC metastasis‐related genes. …”
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Genetic insights into MIS-C Post-COVID-19 in Kuwaiti children: investigating monogenic factors by Mohammed Dashti, Hessa AlKandari, Hessa AlKandari, Md Zubbair Malik, Rasheeba Nizam, Sumi Elsa John, Sindhu Jacob, Arshad Channanath, Fouzeyah Othman, Safa Al-Sayed, Osama Al-Hindi, Mona Al-Mutari, Thangavel Alphonse Thanaraj, Fahd Al-Mulla

“…Demographic and clinical data were collected from medical records, and exome sequencing was performed on the children and their parents to identify rare exonic variants. …”
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CD36 enrichment in HER2-positive mesenchymal stem cells drives therapy refractoriness in breast cancer by Lorenzo Castagnoli, Alma Franceschini, Valeria Cancila, Matteo Dugo, Martina Bigliardi, Claudia Chiodoni, Paolo Toneguzzo, Viola Regondi, Paola A. Corsetto, Filippo Pietrantonio, Serena Mazzucchelli, Fabio Corsi, Antonino Belfiore, Andrea Vingiani, Giancarlo Pruneri, Francesca Ligorio, Mario P. Colombo, Elda Tagliabue, Claudio Tripodo, Claudio Vernieri, Tiziana Triulzi, Serenella M. Pupa

“…Intratumor expression of CSC subsets was evaluated via multiplex immunostaining and immunolocalization analysis. Results Molecular data demonstrated that CD36 is significantly upmodulated on treatment in therapy resistant HER2 + BC patients and its expression levels in BC cells is correlated with FA uptake. …”
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Spatial distribution and long-term trend of wind energy in the Northwest Pacific Ocean by Kaishan Wang, Di Wu, Tao Zhang, Lei Yin, Kai Wu, Chongwei Zheng

“…By analyzing key indicators such as wind power density, effective wind speed occurrence, and energy level occurrence, climate statistics and empirical orthogonal function analysis (EOF) were used to examine the spatial distribution and long-term trend of offshore wind energy resources in the Northwest Pacific. …”
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Experimental Study on Concentration and Size Distribution Characteristics of Particulate Matter in Cold and Hot Rolling by Yanpeng Wu, Meitong Jin, Xiaoyu Li, Junkai Tian, Bin Yang, Junliang Liu, Shujun Zhou, Faming Wang

“…The Rosin–Rammler particle size distribution function was applied to characterize particle size distribution at emission sources. …”
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The role of immune checkpoints PD-1 and CTLA-4 in cardiovascular complications leading to heart failure by Shoulian Huang, Shoulian Huang, Yu Kang, Ting Liu, Yan Xiong, Zixuan Yang, Qing Zhang

“…We conducted a comprehensive analysis using animal models and clinical data to assess the effects of immune checkpoint inhibition on cardiac function. …”
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Development and evaluations of equations to predict empty body weight in pre-weaned hair sheep lambs by C. Jardines-Pozos, R. Salazar-Cuytun, J. Oliva-Hernández, R.J. Leon, R.A. Garcia-Herrera, J.C. Escobar-España, A.L.C. Gurgel, J.C.S. Santana, L.C.V. Ítavo, T.P. Dias-Silva, A.J. Chay-Canul

“…Linear model with intercept, Linear model without intercept and exponential regression models were used for data analysis. The correlation coefficients among SBW and EBW was high (r = 0.89). …”
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Diagnosis and Treatment of Status Epilepticus in a Pediatric Renal Recipient by Gao Hongjun, Luo Xiangdong, Liang Taisheng, Lu Shangguang, Liang FangFang, Dong Yu, Tan Zhen, Wu Zhen

“…We elaborate on the retrospective analysis of clinical data on a patient afflicted with grand mal seizures following a kidney transplant. …”
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Endovascular therapy and medical management of large-core infarcts: prognostic determinants in a retrospective cohort by Yi Li, Yi Li, Hao Tao, Hao Tao, Huan Liu, Huan Liu, Xiang Fan, Xiang Fan, Meng-Yu Zhong, Meng-Yu Zhong, Jie Huang, Neng-Wei Yu, Neng-Wei Yu, Bing-Hu Li, Bing-Hu Li

“…In the EVT subgroup (n = 62), hyperglycemia remained significantly associated with poor outcomes (OR = 1.70, 95% CI: 1.04–2.78, p = 0.034).ConclusionEVT significantly improves functional outcomes in large-core infarcts. Preoperative hyperglycemia and elevated platelet count are independent predictors of poor outcomes. …”
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Development and validation of a polyfit approach for assessing alveolar bone loss using panoramic radiography by Erkang Tian, Jiawei Hong, Zihua Tang, Ruiting Ren, Shuoshun Li, Abbas Ahmed Abdulqader, Mingshan Li, Chaoran Xue, Xianglong Han, Juan Li

“…To evaluate the proportionate bone resorption for each tooth, we employed the PAN-POL model based on the polyfit function to quantify bone loss across six dental sextants and the entire dental arch. …”
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Improvement teaching-learning-based optimization algorithm for solar cell parameter extraction in photovoltaic systems by H. Khaterchi, M. H. Moulahi, A. Jeridi, R. Ben Messaoud, A. Zaafouri

“…We adopt an enhanced single-diode model, integrating modifications into the TLBO algorithm, including dynamic teaching factor adjustment, refined partner selection, and targeted local searches with the fmincon function. Comparative analysis with experimental data from four PV systems validates the model’s accuracy. …”
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Bangladesh Climate Research: The Role of IRBs by Md. Matiur Rahman, Mohammad Mahbub Ur Rahim, Md. Anup Rahman Chowdury, Md. Abdul Mazid, Md. Amirul Islam, Md. Kaoser Bin Siddique

“…This study systematically reviewed peer-reviewed journals, conference papers, institutional reports, and policy documents published within the last decade, focusing on ethical challenges in climate studies and the function of IRBs. Thematic analysis revealed key areas: the paramount importance of research ethics (including informed consent and privacy), the multifaceted impacts of climate change and adaptation strategies, the complexities and challenges faced by IRBs (especially in developing countries), the critical consideration of vulnerability in research participants, the issue of corruption in adaptation efforts, and the necessity of effective stakeholder engagement. …”
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Reliability Estimation for the Gull Alpha Power Pareto Model Under Progressive Type-I Censoring by Mahmoud M. Abdelwahab, Ahmed R. El-Saeed, M. A. Abdelkawy, Mohamed Khalifa Ahmed Issa, Hanem Mohamed

“…A comprehensive statistical analysis of the GAPP distribution was performed, including the estimation of its parameters and the reliability function. …”
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Fuzzy Modeling to Predict Severely Depressed Left Ventricular Ejection Fraction following Admission to the Intensive Care Unit Using Clinical Physiology by Rúben Duarte M. A. Pereira, Cátia M. Salgado, Andre Dejam, Shane R. Reti, Susana M. Vieira, João M. C. Sousa, Leo A. Celi, Stan N. Finkelstein

“…To the best of the authors’ knowledge, this is the first study predicting severely impaired LVEF using multivariate analysis of routinely collected data in the ICU. We recommend inclusion of these findings into triaged management plans that balance urgency with resources and clinical status, particularly for reducing the time of echocardiographic examination.…”
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A genome‐scale yeast library with inducible expression of individual genes by Yuko Arita, Griffin Kim, Zhijian Li, Helena Friesen, Gina Turco, Rebecca Y Wang, Dale Climie, Matej Usaj, Manuel Hotz, Emily H Stoops, Anastasia Baryshnikova, Charles Boone, David Botstein, Brenda J Andrews, R Scott McIsaac

“…We observed that strains with inducible essential genes that have low native expression can often grow without inducer. Analysis of data from eukaryotic and prokaryotic systems shows that native expression is a variable that can bias promoter‐perturbing screens, including CRISPRi. …”
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Associations between milk infrared-predicted plasma biomarkers of stress resilience and fertility in dairy cattle: Insights for enhancing breeding programs and herd management by Alessio Cecchinato, Hugo Toledo-Alvarado, Lucio Flavio Macedo Mota, Vittoria Bisutti, Erminio Trevisi, Riccardo Negrini, Sara Pegolo, Stefano Schiavon, Luigi Gallo, Giovanni Bittante, Diana Giannuzzi

“…The blood metabolites (15 blood biomarkers related to hepatic damage and function, oxidative stress, inflammation, and innate immunity) were predicted using milk Fourier-transform mid-infrared (MIR) spectroscopy. …”
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