Revealing tumor microenvironment communication through m6A single-cell analysis and elucidating immunotherapeutic potentials for cutaneous melanoma (CM) by Lun Liu, Maxwell Andriano Kishengere, Xueming Xu, Zhanghui Yue

“…Methods Using Non-negative matrix factorization (NMF) analysis on single-cell RNA-seq data (GSE215121) from three CM samples obtained from public databases, 26 m6A RNA methylation regulators were utilized to determine TME subclusters, their expression, and function. …”
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Comprehensive techno-environmental evaluation of an isolated PV/wind/biomass hybrid microgrid employing various battery technologies: A comparative analysis. by Mohammed Alqahtani, Saeed Alhajri, Ahmed S Menesy, Ali Maher Mohammed, Hamdy M Sultan, Muhammad Khalid

“…This paper presents a comprehensive techno-economic analysis of an off-grid PV/wind/biomass hybrid system. …”
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Genome-Wide Analysis of Wound-Induced Polypeptide Genes in <i>Glycine max</i> and Their Expression Dynamics During Cyst Nematode Infection by Wenshu Kang, Zicheng Sun, Jiayao Xu, Nawei Qi, Piao Lei

“…These findings suggest that <i>GmWIP</i> genes may act as regulators in the defense responses of susceptible soybean cultivars, providing a foundation for future functional studies.…”
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In silico modeling and analysis of squalene synthase-like 1 (SSL-1) enzyme from green microalga Botryococcus terribilis AICB 872 by Tiberiu SZÖKE-NAGY, Sebastian Alin PORAV, Nicolae DRAGOȘ

“…The present study aimed to investigate the structural features of SSL-1 enzyme involved in the biosynthesis of presqualene diphosphate from B. terribilis AICB 872, and its functional conservation by means of computational proteomics and molecular biology techniques. …”
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Prognostic analysis and mechanistic exploration of the autophagy-related exosome genes ITGA3, ITGB4, and PTK6 in pancreatic ductal adenocarcinoma by Lunbing Liang, Pengcheng Guo, Mingming Xiao, Fuqiang Zu, Guoliang Hao, Chaochun Zhang, Qingfeng Liu

“…Matrix files of 178 PC tissues (including 143 PDAC) and 167 healthy human pancreatic tissues and clinical data were downloaded from the UCSC Xena database, exosome database (exoRbase) and autophagy database (HADb), and the relevant mechanisms were explored by bioinformatics methods, including differential analysis, LASSO regression analysis, survival analysis, functional analysis, immune infiltration analysis and drug sensitivity analysis. …”
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Genome-Wide Identification, Characterization, and Expression Analysis of the NAC Transcription Factor Family in Sweet Cherry (<i>Prunus avium</i> L.) by Feng An, Xin Yin, Kaibire Jueraiti, Yuanyuan Yang, Zhuoyang Yan, Jie Li, Dongqian Shan

“…These findings provide valuable insights for future research on the functional characteristics of the <i>PaNAC</i> genes in the growth, development, and responses to abiotic stress in sweet cherry.…”
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Comparative transcriptome analysis of pectoralis major muscles affected by white striping, wooden breast and spaghetti meat in male and female broiler chickens by Marija Bošković Cabrol, Marianna Pauletto, Mery Giantin, Mauro Dacasto, Gerolamo Xiccato, Angela Trocino

“…This study aimed to compare transcriptomic profiles of normal and defective pectoralis major muscles to identify biological pathways underlying male and female myopathies. Transcriptomic analysis was performed on RNA-seq data from pectoralis major muscles of male and female Ross 308 broilers. …”
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Global transcriptome analysis of orange wheat blossom midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) to identify candidate transcripts regulating diapause. by Zhong-Jun Gong, Yu-Qing Wu, Jin Miao, Yun Duan, Yue-Li Jiang, Tong Li

“…<h4>Conclusions/significance</h4>The OWBM transcriptome greatly improves our genetic understanding and provides a platform for functional genomics research of this species. The DGE profiling data provides comprehensive information at the transcriptional level that facilitates our understanding of the molecular mechanisms of various physiological aspects including development and diapause stages in OWBM. …”
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The Effectiveness of Conservative Management for Acute Whiplash Associated Disorder (WAD) II: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. by Taweewat Wiangkham, Joan Duda, Sayeed Haque, Mohammad Madi, Alison Rushton

“…<h4>Design</h4>Systematic review and meta-analysis of Randomised Controlled Trials (RCTs) using a pre-defined protocol. …”
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Mortality rates in physician staffed ground vs. air ambulance for severe trauma patients: retrospective analysis of the Japanese nationwide trauma registry by Tomohiro Akutsu, Akira Endo, Ryo Yamamoto, Kazuma Yamakawa, Hiraaki Okuzawa, Keisuke Suzuki, Hiromasa Hoshi, Yasuhiro Otomo, Koji Morishita

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Association between cyclin-dependent kinase 4/6 inhibitors and nephrotoxicity in patients with breast cancer: A Systematic Review and meta-analysis by Jiayong Cui, Jinquan Sun, Xueying Zhou, Yi Li, Jiuda Zhao, Guoshuang Shen

“…Subgroup analyses revealed varying risks among 4 different CDK 4/6 inhibitors. Furthermore, analysis of FDA Adverse Event Reporting System data corroborated these findings, emphasizing the occurrence of nephrotoxicity in real-world settings. …”
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Efficacy and safety of belimumab therapy for patients with lupus nephritis: A meta‐analysis and a propensity score‐matched case–control study by Kai Zhang, Tiening Qi, Donghua Guo, Youxia Liu

“…All participants were followed up for a duration exceeding 96 weeks to evaluate the outcomes of the treatments. Results Our meta‐analysis incorporated data from three articles involving a total of 666 patients. …”
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Towards the Real-World Analysis of Lumbar Spine Standing Posture in Individuals with Low Back Pain: A Cross-Sectional Observational Study by Elena Muñoz-Gómez, Frederick McClintock, Andrew Callaway, Carol Clark, Raee Alqhtani, Jonathan Williams

“…A three-stage design was used, (i) Verification of a bespoke algorithm characterising lumbar standing behaviour, (ii) Day-long assessment of standing behaviours of individuals with posture-related low back discomfort, and (iii) Case study application to individuals with clinical LBP. Analysis of standing posture across time included variability, fidgeting, and amplitude probability distribution function analysis. …”
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Model Validation and Sensitivity Analysis of Coupled Non‐Equilibrium Heat and Mass Transfer in Porous Media With Application to Evaporation From Bare Soils by Ashkan Talebi, Brent E. Sleep, Denis M. O'Carroll

“…Incorporating the atmospheric stability function accounting for real‐time atmospheric conditions did not improve the predictive capability for the simulated drying events compared to the case when only a neutral atmospheric condition was implemented. …”
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Combined analysis of embryonic gonadal development differences of mulard duck and muscovy duck using long non-coding RNAs and mRNAs by LI Li, ZHANG Linli, XIN Qingwu, MIAO Zhongwei, ZHU Zhiming, QIU Junzhi, HAO Xiaona, HUANG Qinlou, ZHENG Nenzhu

“…Three male embryonic gonadal tissues of the mulard duck (BF₁, BF₂, BF₃) and the muscovy duck (F₁, F₂, F₃) were collected to extract RNA and perform high-throughput sequencing, and differential genes and lncRNAs were screened to predict target genes and perform functional annotations. Finally, the sequencing data were verified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). …”
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Does the Ischemic Core Really Matter? An Updated Systematic Review and Meta‐Analysis of Large Core Trials After TESLA, TENSION, and LASTE by Mohammad AlMajali, Mahmoud Dibas, Malik Ghannam, Milagros Galecio‐Castillo, Abdullah Al Qudah, Farid Khasiyev, Juan Vivanco‐Suarez, Aaron Rodriguez‐Calienes, Mudassir Farooqui, Sophie L. Shogren, Fawaz AlMajali, Albert Yoo, Edgar Samaniego, Tudor Jovin, Amrou Sarraj, Santiago Ortega‐Gutierrez

“…We aimed to study the efficacy and safety of EVT in patients with acute ischemic stroke with large core and stratify by several subgroups including core infarct at presentation, using a comprehensive meta‐analysis of aggregate data. Methods We executed a systematic search to identify randomized controlled trials that compared EVT to medical management (MM) for the treatment of patients with acute ischemic stroke with large core, defined as Alberta Stroke Program Early CT [Computed Tomography] Score ≤5 on noncontrast CT and/or estimated ischemic core ≥50 mL on CT‐perfusion/MR diffusion. …”
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PMAIP1-mediated glucose metabolism and its impact on the tumor microenvironment in breast cancer: Integration of multi-omics analysis and experimental validation by Yidong Zhang, Hang Xu, Xuedan Han, Qiyi Yu, Lufeng Zheng, Hua Xiao

“…Thus, this study aimed to develop a glucose metabolism–tumor microenvironment score to provide new perspectives on breast cancer treatment. Method: Data were acquired from the Gene Expression Omnibus and UCSC Xena databases, and glucose-metabolism-related genes were acquired from the Gene Set Enrichment Analysis database. …”
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DSA-based perfusion parameters versus TICI score after mechanical thrombectomy in acute ischaemic stroke patients: a congruence analysis by Sebastian R. Reder, Andrea Kronfeld, Sonja Gröschel, Arda Civelek, Klaus Gröschel, Marc A. Brockmann, Timo Uphaus, Marianne Hahn, Carolin Brockmann, Ahmed E. Othman

“…Methods From DSA image data (n = 90; 38 females; age 73.3 ± 13.1 years [mean ± standard deviation]), time-contrast agent (CA) concentration curves were acquired, and maximum slope (MS), time to peak (TTP), and maximum CA concentration (CAmax) were calculated using an arterial input function. …”
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Machine learning-based transcriptmics analysis reveals BMX, GRB10, and GADD45A as crucial biomarkers and therapeutic targets in sepsis by Yanwei Cheng, Haoran Peng, Qiao Chen, Lijun Xu, Lijie Qin

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Safety and Efficacy of Combination Therapy with Anti-Vascular Endothelial Growth Factor and Laser for Retinopathy of Prematurity: A Systematic Review and Meta-Analysis by Alamoudi A, Alnabihi AN, Alsudais AS, Almufarriji N, Batais WT, Aldahlawi AK, Altalhi A, Al-Dhibi HA, Alakeely AG

“…Anas Alamoudi,1 Ahmed N Alnabihi,2,3 Ali Saleh Alsudais,2,3 Naif Almufarriji,1 Waleed T Batais,2,3 Abdulaziz Khalid Aldahlawi,2,3 Abdullah Altalhi,4 Hassan A Al-Dhibi,5 Adel G Alakeely6 1Department of Ophthalmology, Jeddah Eye Hospital, Jeddah, Saudi Arabia; 2College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia; 3King Abdullah International Medical Research Center, Jeddah, Saudi Arabia; 4Division of Vitreoretinal Surgery, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia; 5Division of Vitreoretinal Surgery and Uveitis, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia; 6Magrabi Hospitals and Centers, Riyadh, Saudi ArabiaCorrespondence: Anas Alamoudi, Department of Ophthalmology, Jeddah Eye Hospital, Jeddah, Saudi Arabia, Email anasalamoudi99@gmail.comAim: This systematic review and meta-analysis aimed to evaluate the safety and efficacy of combined laser and anti-VEGF therapy for (retinopathy of prematurity ROP), focusing on both structural and functional outcomes.Methods: A comprehensive search was conducted in multiple databases to identify randomized controlled trials (RCTs) that investigated combination therapy for ROP. …”
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