High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform by Sascha D. Braun, Sascha D. Braun, Martin Reinicke, Martin Reinicke, Celia Diezel, Celia Diezel, Elke Müller, Elke Müller, Katrin Frankenfeld, Thomas Schumacher, Hugo Arends, Stefan Monecke, Stefan Monecke, Ralf Ehricht, Ralf Ehricht, Ralf Ehricht

“…Given the urgent clinical need for rapid diagnostics, immunochromatographic assays represent a promising and urgently needed approach for economic and available point-of-care detection. However, the development of such assays is often hindered by the time-consuming process of identifying high-affinity antibody pairs.MethodsTo accelerate this process, we evaluated a protein microarray platform as a high-throughput screening tool to identify optimal monoclonal antibody (mAb) pairs targeting the most clinically relevant carbapenemases. …”
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Identification of Critical Signature in Post‐Traumatic Stress Disorder Using Bioinformatics Analysis and in Vitro Analyses by Lifen Liu, Yang Liu, Rui Li, Yue Teng, Shuyang Zhao, Jinhong Chen, Changjiang Li, Xinyu Hu, Lin Sun

“…Current research is focused on identifying effective treatments for PTSD. Advances in bioinformatics provide opportunities to elucidate the underlying mechanisms of PTSD. …”
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Possibility of the Use of Public Microarray Database for Identifying Significant Genes Associated with Oral Squamous Cell Carcinoma by Ki-Yeol Kim, In-Ho Cha

“…Most studies include insufficient samples to apply statistical methods for detecting significant gene sets. This study combined two small microarray datasets from a public database and identified significant genes associated with the progress of oral squamous cell carcinoma. …”
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A High Phosphorus Diet Affects Lipid Metabolism in Rat Liver: A DNA Microarray Analysis. by Sunwoo Chun, Takeshi Bamba, Tatsuya Suyama, Tomoko Ishijima, Eiichiro Fukusaki, Keiko Abe, Yuji Nakai

“…In the present study, we performed DNA microarray analysis to investigate the effects of a HP diet on the liver, which plays a pivotal role in energy metabolism. …”
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Development of a microarray based telomerase binding assay reveals unusual binding of a cytochalasin derivative by Jia Li Ye, Lu Fan, Christian Bär, Thomas Thum, Oliver Plettenburg, Russell J. Cox, Carsten Zeilinger

“…In this study, we present a highly miniaturized, microarray-based assay designed to identify compounds that target telomerase. …”
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In Vitro Investigation of Statin Effects on Genes Associated with Severe COVID-19 in Cancerous and Non-Cancerous Cells by Adriana Kapustová, Patrik Macášek, Bibiána Baďurová, Jana Melegová, Silvie Rimpelová, Jan Kubovčiak, Jana Šáchová, Miluše Hradilová, Michal Kolář, Libor Vítek, Tomáš Ruml, Helena Gbelcová

“…However, statins exhibit additional pleiotropic effects. The present study aims to investigate the effects of all eight currently existing statins on the expression of genes whose products have been reported to be directly associated with complicated COVID-19 disease. …”
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Systematic spatial bias in DNA microarray hybridization is caused by probe spot position-dependent variability in lateral diffusion. by Doris Steger, David Berry, Susanne Haider, Matthias Horn, Michael Wagner, Roman Stocker, Alexander Loy

“…Numerical simulations reveal a strong influence of microarray well geometry on the spatial bias.<h4>Conclusions</h4>Reciprocal adjustment of the size of the microarray hybridization chamber to the area of surface-bound probes is a simple and effective measure to minimize or eliminate the diffusion-based bias, resulting in increased uniformity and accuracy of quantitative DNA microarray hybridization.…”
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Genetic findings of children with congenital heart diseases using chromosomal microarray and trio-based whole exome sequencing by Rui Guo, Chunhong Duan, Mehdi Zarrei, Miriam S. Reuter, Rui Dong, Guangye Zhang, Xiaomeng Yang, Haiyang Zhang, Ying Wang, Stephen W. Scherer, Yi Liu, Zhongtao Gai

“…In individuals with extracardiac features, the overall detection rate was significantly higher (61.5%) than in individuals with isolated CHD (17.3%) (P = 4.6 × 10− 3). …”
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Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy by Jens Dannull, Chunrui Tan, Christine Farrell, Cynthia Wang, Scott Pruitt, Smita K. Nair, Walter T. Lee

“…Dendritic cell- (DC-) tumor fusion cells stimulate effective in vivo antitumor responses. However, therapeutic approaches are dependent upon the coadministration of exogenous 3rd signals. …”
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Deciphering the prokaryotic community and metabolisms in South African deep-mine biofilms through antibody microarrays and graph theory. by Yolanda Blanco, Luis A Rivas, Antonio García-Moyano, Jacobo Aguirre, Patricia Cruz-Gil, Arantxa Palacín, Esta van Heerden, Víctor Parro

“…Both culture-independent techniques coincided in detecting features related to aerobic sulfur-oxidizers, aerobic chemoorganotrophic Alphaproteobacteria and metanotrophic Gammaproteobacteria. 16S rRNA gene sequencing detected phylotypes related to nitrate-reducers and anaerobic sulfur-oxidizers, whereas the EMChip66 detected immunological features from methanogens and sulfate-reducers. …”
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A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases by Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Karthik Krishna, Tianhao Wang, Kang Bei, Chithra Changalath, Shiny Matilda, Alex J. Rai, Renata Welc-Falęciak, Agnieszka Pawełczyk, Lucas S. Blanton, Aleš Chrdle, Andrea Fořtová, Daniel Růžek, Gheyath K. Nasrallah, Laith J. Abu-Raddadi, Duaa W. Al-Sadeq, Marah Abed Alhakim Abdallah, Daniele Lilleri, Chiara Fornara, Piera D’Angelo, Milena Furione, Maria Söderlund-Venermo, Klaus Hedman, Dimosthenis Chochlakis, Eirini Makridaki, Artemis Ntoula, Anna Psaroulaki, Angélica Escárcega-Ávila, John J. Rajasekaran

“…The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple pathogens simultaneously. …”
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No Amplifications of Hypoxia-Inducible Factor-1α Gene in Invasive Breast Cancer: A Tissue Microarray Study by Marije M. Vleugel, Reinhard Bos, Horst Buerger, Petra van der Groep, Outi R. Saramäki, Tapio Visakorpi, Elsken van der Wall, Paul J. van Diest

“…Methods: Protein and gene expression of HIF‐1α were analyzed on a tissue microarray of 94 breast cancers by immunohistochemistry and fluorescent in situ hybridization (FISH), respectively. …”
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Consistency and Stability in Feature Selection for High-Dimensional Microarray Survival Data in Diffuse Large B-Cell Lymphoma Cancer by Kazeem A. Dauda, Rasheed K. Lamidi

“…High-dimensional survival data, such as microarray datasets, present significant challenges in variable selection and model performance due to their complexity and dimensionality. …”
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PD-L1 quantification across tumor types using the reverse phase protein microarray: implications for precision medicine by Mariaelena Pierobon, Johann De Bono, Ruth Riisnaes, Giuseppe Giaccone, Matthew Blackburn, Lance Liotta, Vienna Ludovini, Neil J Shah, Giuseppina Improta, Antonella Ravaggi, Niven Mehra, Angelo Sidoni, Elisa Baldelli, K Alex Hodge, Guido Bellezza, Guido Gambara, Martina Mandarano, Chamodya Ruhunusiri, Bryant Dunetz, Maysa Abu-Khalaf, Julia Wulfkuhle, Rosa I Gallagher, Franco Odicino, Maria Isabella Sereni, Angela Zupa, Perry Demsko, Lucio Crino', Emanuel F Petricoin

“…Using a high throughput immunoassay, the reverse phase protein microarray (RPPA), coupled with a fluorescence-based detection system, this study compared the performance of six anti-PD-L1 antibody clones on 666 tumor samples.Methods PD-L1 expression was measured using five antibody clones (22C3, 28–8, CAL10, E1L3N and SP142) and the therapeutic antibody atezolizumab on 222 lung, 71 ovarian, 52 prostate and 267 breast cancers, and 54 metastatic lesions. …”
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Substantia nigra and blood gene signatures and biomarkers for Parkinson’s disease from integrated multicenter microarray-based transcriptomic analyses by Hui-Hui Fan, Na-Na Hou, Na-Na Hou, Dao-Lu Zhang, Dan-Ni Liu, Rong-Ting Tang, Hai-Tao Luo, Ya-Dan Song, Lei Cui, Xiong Zhang, Jian-Hong Zhu, Jian-Hong Zhu

“…PD diagnosis depends on clinical manifestation of symptoms but is lack of effective biomarker.MethodsAvailable human microarray-based transcriptomic datasets of the substantia nigra and blood were acquired for PD cases and controls. …”
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Critical evaluation of the reliability of DNA methylation probes on the Illumina MethylationEPIC v1.0 BeadChip microarrays by Wei Zhang, Juan I. Young, Lissette Gomez, Michael A. Schmidt, David Lukacsovich, Achintya Varma, X. Steven Chen, Brian Kunkle, Eden R. Martin, Lily Wang

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Local Mean Normalization of Microarray Element Signal Intensities across an Array Surface: Quality Control and Correction of Spatially Systematic Artifacts by Carlo Colantuoni, George Henry, Scott Zeger, Jonathan Pevsne

“…Here we present a methodology for the normalization of element signal intensities to a mean intensity calculated locally across the surface of a DNA microarray. These methods allow the detection and/or correction of spatially systematic artifacts in microarray data. …”
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CD14 and complement crosstalk and largely mediate the transcriptional response to Escherichia coli in human whole blood as revealed by DNA microarray. by Corinna Lau, Ståle Nygård, Hilde Fure, Ole Kristoffer Olstad, Marit Holden, Knut Tore Lappegård, Ole-Lars Brekke, Terje Espevik, Eivind Hovig, Tom Eirik Mollnes

“…Combined inhibition of a key complement component (C3 and C5) and TLR-co-receptor CD14 has been shown to attenuate certain systemic inflammatory responses. Using DNA microarray and gene annotation analyses, we aimed to decipher the effect of combined inhibition of C3 and CD14 on the transcriptional response to bacterial challenge in human whole blood. …”
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Prenatal diagnosis and molecular cytogenetic analysis of pure chromosome 10p15.3 microdeletion using chromosomal microarray analysis by Na Zhang, Nan Huang, Yu’e Chen, Xinying Chen, Jianlong Zhuang

“…Karyotyping and chromosomal microarray analysis (CMA) was conducted to assess chromosomal abnormalities and detect copy number variations (CNVs) within the families, respectively. …”
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A microarray assessment of the modulation of human oxidative stress gene expression by rooibos and honeybush tea extracts in UVB-irradiated keratinocytes by Lana Keet, Nashia Deepnarain, Danicke Willemse, Stefan Abel, Ann Louw, Mariska Lilly

“…Microarray was used to investigate the expression of 84 oxidative stress response genes in response to rooibos and honeybush tea extracts and the effect of pre-exposure to extracts prior to UVB exposure was assessed. …”
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