Spheroid Culture of Mesenchymal Stem Cells by Zoe Cesarz, Kenichi Tamama

“…Mesenchymal stem cells (MSCs) cultured in spheroids have enhanced anti-inflammatory, angiogenic, and tissue reparative/regenerative effects with improved cell survival after transplantation. …”
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Pharmacologic inhibition of CSF-1R suppresses intrinsic tumor cell growth in osteosarcoma with CSF-1R overexpression by Cheng Dai, Bin Shen, Shenyan Liu, Cong Li, Shuqun Yang, Jie Wang, Jie Zhang, Manqi Liu, Zhixuan Zhu, Wan Shi, Qi Zhang, Zhui Chen, Nannan Zhang

“…Immunohistochemistry (IHC) was utilized to analyze human tissue microarray samples of osteosarcoma. We then investigated the anti-tumor effect and the mechanisms of action of pharmacologic inhibition of CSF-1R activity by pimicotinib (ABSK021), a highly potent and selective small molecule inhibitor of CSF-1R, in osteosarcoma models both in vitro and in vivo. …”
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Prenatal Diagnosis of 17p13.1p13.3 Duplication by Kirsi Kiiski, Tiiu Roovere, Riina Zordania, Harriet von Koskull, Nina Horelli-Kuitunen

“…This was further defined as a chromosome 17p13.1p13.3 duplication by FISH and genomic microarray analysis (GMA). In addition Prenatal BACs-on-Beads (PN_BoBs) assay was performed, which detected the duplication clearly. …”
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Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue by Isis Campos Prado, João Pedro Rangel da Silva Carvalho, André Souza Araujo, Paloma Napoleão-Pêgo, Salvatore Giovanni De-Simone

“…An identified linear B-cell epitope (DENV/18) specific for DENV 1–4 serotypes recognized by IgG in patient sera was selected as a target molecule after a microarray of peptides using the SPOT-synthesis methodology. …”
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QKI-induced circ_0001766 inhibits colorectal cancer progression and rapamycin resistance by miR-1203/PPP1R3C/mTOR/Myc axis by Yulai Zhou, Yan Gao, Yinghui Peng, Changjing Cai, Ying Han, Yihong Chen, Gongping Deng, Yanhong Ouyang, Hong Shen, Shan Zeng, Yangfeng Du, Zemin Xiao

“…However, no circRNA-based therapies have yet entered clinical development, underscoring the need for comprehensive detection and mechanistic studies of circRNAs in CRC. …”
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Uncovering genetic contributors to developmental delay and intellectual disability: a focus on CNVs in pediatric patients by Yilun Tao, Yilun Tao, Hongzhi Guo, Dong Han, Miao Yang, Ting Lun, Lihong Wang, Wenxia Song, Haiwei Wang, Xiaoze Li

“…BackgroundDevelopmental delay (DD) and intellectual disability (ID) are prevalent in children and often have genetic causes, particularly copy number variations (CNVs). Chromosomal microarray analysis (CMA) and whole-exome sequencing (WES) are key diagnostic tools for identifying genetic contributions to these disorders. …”
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Identification of genes and pathways related to lymphovascular invasion in breast cancer patients: A bioinformatics analysis of gene expression profiles by Sukhontip Klahan, Henry Sung-Ching Wong, Shih-Hsin Tu, Wan-Hsuan Chou, Yan-Feng Zhang, Thien-Fiew Ho, Chih-Yi Liu, Shih-Ying Yih, Hsing Fang Lu, Sean Chun-Chang Chen, Chi-Cheng Huang, Wei-Chiao Chang

“…Surgery is the most effective treatment for breast cancer patients. However, some patients developed recurrence and distant metastasis after surgery. …”
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Mechanism-based screen for G1/S checkpoint activators identifies a selective activator of EIF2AK3/PERK signalling. by Simon R Stockwell, Georgina Platt, S Elaine Barrie, Georgia Zoumpoulidou, Robert H Te Poele, G Wynne Aherne, Stuart C Wilson, Peter Sheldrake, Edward McDonald, Mathilde Venet, Christelle Soudy, Frédéric Elustondo, Laurent Rigoreau, Julian Blagg, Paul Workman, Michelle D Garrett, Sibylle Mittnacht

“…Transcriptional profiling by cDNA microarray combined with reverse genetics revealed phosphorylation of the eukaryotic initiation factor 2-alpha (EIF2A) through the eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3/PERK) as the mechanism of action of this compound. …”
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Homeobox protein B6 and homeobox protein B8 control immune-cancer cell interactions in pancreatic cancer by Ludivine Bertonnier-Brouty, Sara Bsharat, Kavya Achanta, Jonas Andersson, Thanya Pranomphon, Tania Singh, Tuomas Kaprio, Jaana Hagström, Caj Haglund, Hanna Seppänen, Rashmi B. Prasad, Isabella Artner

“…Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer lacking effective drugs and therefore new treatment targets are needed. …”
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Upregulation of RIG‐I is Critical for Responsiveness to IFN‐α Plus Anti‐PD‐1 in Colorectal Cancer by Haihang Nie, Shilin Fang, Rui Zhou, Yifan Jia, Jingkai Zhou, Yumei Ning, Yali Yu, Yuntian Hong, Fei Xu, Qiu Zhao, Jiayan Nie, Fan Wang

“…ABSTRACT Backgrounds Immunotherapy is a promising and effective approach that has achieved significant curative effects in colorectal cancer (CRC). …”
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Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine by Guanguan Qiu, Ruoyang Zhang, Huifeng Qian, Ruoqiong Huang, Jie Xia, Ruoxi Zang, Zhenkai Le, Qiang Shu, Jianguo Xu, Guoping Zheng, Jiangmei Wang

“…COVID-19 vaccination is the most effective strategy for preventing severe disease and death. …”
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Mutation spectrum in epilepsy and seizure disorders in a group of Emirati patients: a retrospective cohort study and literature review by Aisha AlShamsi, Sema Koruturk

“…The genetic tests were performed based on the patient's clinical phenotypes and family history, and included chromosome microarray, targeted variant testing, next-generation sequencing, or whole-exome sequencing (solo or trio). …”
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USP5 Promotes Head and Neck Squamous Cell Carcinoma Progression via mTOR Signaling Pathway by Ni Xiong, Yue Wang, Junhong Jiang

“…Validation was conducted using tissue microarray (TMA) analysis and immunohistochemistry (IHC) to compare USP5 expression levels in HNSCC tissues versus adjacent normal tissues. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Methods We employed microarray and single-cell RNA sequencing (scRNA-seq) to investigate gene expression changes and immune cell dynamics in mice following crush injury at 3 and 7 days post-injury (dpi). …”
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Application of family whole-exome sequencing for prenatal diagnosis—an analysis of 357 cases by Yijun Ge, Huizhen Yuan, Yao Yu, Junfang Xiao, Danping Liu, Yongbao Peng, Ying Liu, Shuhui Huang, Bicheng Yang, Yongyi Zou, Yanqiu Liu

“…Using techniques such as amniotic fluid, chorionic villus, or umbilical cord blood sampling, intact fetal cells were extracted for cell culture and subsequently analyzed using chromosomal karyotyping and chromosomal microarray techniques. 2. With fully informed consent, fetuses and their parents whose genetic etiology could not be detected by karyotyping combined with chromosomal microarray technology had their cellular DNA subjected to whole-exome sequencing of the pedigree. 3. …”
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Hypoxia alters the response of ovarian cancer cells to the mitomycin C drug by Aleksandra Gawrylak, Aleksandra Gawrylak, Klaudia Brodaczewska, Roksana Iwanicka-Nowicka, Roksana Iwanicka-Nowicka, Marta Koblowska, Marta Koblowska, Agnieszka Synowiec, Lubomir Bodnar, Lubomir Bodnar, Cezary Szczylik, Bogdan Lesyng, Rafał Stec, Claudine Kieda, Claudine Kieda

“…In this study, we show that mitomycin C (MMC), an antineoplastic antibiotic, is rarely used in ovarian cancer (OC) treatment despite its potential efficacy; we use MMC as an example of a treatment that warrants reevaluation under microenvironmental conditions, particularly during in vitro testing.MethodsTo evaluate the effects of MMC and oxygen tension (pO2) on OC cells (SKOV3), HTA 2.0 microarrays were used, which demonstrated that hypoxia and MMC induced transcriptomic changes in OC cells. …”
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Robust modeling of differential gene expression data using normal/independent distributions: a Bayesian approach. by Mojtaba Ganjali, Taban Baghfalaki, Damon Berridge

“…In this paper, the problem of identifying differentially expressed genes under different conditions using gene expression microarray data, in the presence of outliers, is discussed. …”
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Yield of Genetic Testing in Children with Autism Spectrum Disorder – A Single-Center Experience by Kriti Arora, Minakshi Balwani, Akash Wakchaure, Gouri R Passi

“…Yield of whole exome sequencing (WES), 12/27 (44.49%), was more than that of chromosomal microarray (CMA) 1/30 (3.3%) (P < 0.05). Fragile-X gene mutation was detected in 0/14 patients tested. …”
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High-Resolution Genomic Profiling of Chromosomal Abnormalities in Human Stem Cells Using the 135 K StemArray by Aaron M. Elliott, Kristi A. Hohenstein Elliott, Anja Kammesheidt

“…Determining the copy number variant (CNV) profile of stem cell lines is critical since CNVs can have dramatic effects on gene expression and tumorigenic potential. …”
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Exploring the Functional Disorder and Corresponding Key Transcription Factors in Intraductal Papillary Mucinous Neoplasms Progression by Guiying Bai, Chenxuan Wu, Yingtang Gao, Guiming Shu

“…This study has analyzed the gene expression patterns of an IPMN microarray dataset including normal pancreatic ductal tissue (NT), intraductal papillary mucinous adenoma (IPMA), intraductal papillary mucinous carcinoma (IPMC), and invasive ductal carcinoma (IDC) samples. …”
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